Abstract Herbicides have been recognized as the main environmental factor associated with human neurodegenerative disorders such as Parkinson's disease(PD). Previous studies indicated that the ...exposure to glyphosate, a widely used herbicide, is possibly linked to Parkinsonism, however the underlying mechanism remains unclear. We investigated the neurotoxic effects of glyphosate in differentiated PC12 cells and discovered that it inhibited viability of differentiated PC12 cells in dose-and time-dependent manners. Furthermore, the results showed that glyphosate induced cell death via autophagy pathways in addition to activating apoptotic pathways. Interestingly, deactivation of Beclin-1 gene attenuated both apoptosis and autophagy in glyphosate treated differentiated PC12 cells, suggesting that Beclin-1 gene is involved in the crosstalk between the two mechanisms.
Ce0.8Sm0.1Nd0.1O2-δ (SNDC) is one of the most promising electrolyte materials for intermediate-temperature solid oxide fuel cells (IT-SOFCs). However, the adverse effect on the preparation of the ...electrolyte due to the high sintering temperature of SNDC limits the application of SNDC as an electrolyte material in IT-SOFCs. In this work, x mol % (x = 0, 0.5, 1.0, 1.5, 2.0, 2.5) Fe-doped SNDC (xFe-SNDC) powders were firstly fabricated by sol-gel synthesis, and then the corresponding bulks were prepared by cold-pressing and sintering at 1200∼1400 °C. The effects of Fe-doping on the sintering capability and electrical conductivity of SNDC were investigated. The results showed that Fe-doping leads to significant improvements in both the relative density and the electrical conductivity of SNDC. Doping Fe reduced the sintering temperature of SNDC from 1400 °C to 1200 °C and the relative density of 0.5Fe-SNDC sintered at 1200 °C reached 95.85%. Moreover, 0.5Fe-SNDC possessed the maximum conductivity of 0.0674 S cm−1 at 750 °C, which was attributed to the highest density and the highest conductivity of grain plus grain boundary.
•Fe2O3-doped Ce0.8Sm0.1Nd0.1O2-δ materials were synthesized by sol-gel and calcination.•The dopant content affects the structure and ionic conductivity of sintered bulks.•Doping Fe reduced the sintering temperature of SNDC from 1400 °C to 1200 °C.•0.5mol% Fe-doped Ce0.8Sm0.1Nd0.1O2-δ bulk exhibits the maximum ionic conductivity.
The uncontrollable migration of cancer cells is one of the reasons for the poor prognosis. The interaction between collagen and cellular processes is usually dominated by the full triple‐helix ...structure of collagen. However, it remains to be explored how the collagen configuration affects the migration behaviors of cancer cells. Michigan cancer foundation‐7 cells are chosen to study this relationship. Meanwhile, the grass carp is one of the representative fish species in Hubei and is virtually unaffected by zoonotic diseases. Therefore, grass carp‐derived collagen is prepared and used to modulate the migration behaviors. The collagen sample is pretreated via several procedures including the hydrothermal process and EDC/NHS, respectively. The underlying mechanisms affecting migration behavior are explored by tuning the degree of denaturation, assembly states, and cross‐linking conditions of collagen. Results demonstrate that moderate cross‐linking and assembly contribute to the migration processes, while denaturation significantly suppresses migration. Of particular note is the positive correlation of F‐actin expression levels with these phenomena. More importantly, the hydrophilic and hydrophobic properties of the collagen surface also affect the migration process. In summary, the study may provide insights to flexibly tune the migration behavior of cancer cells, which may be promising in inhibiting tumor metastasis.
The relationship between the configuration of grass crap‐derived collagen and migration behavior of cancerous cells is studied in this article. Results confirm that denaturation inhibits migration, while cross‐linking and assembly contribute to the migration of michigan cancer foundation‐7 cells. This study may provide an opportunity for exploring the utilization value of collagen‐based biomaterials for inhibiting tumor metastasis.
Background
This phase II study evaluated camrelizumab in different PD-L1 expression cohorts of patients with previously treated advanced/metastatic non-small cell lung cancer (NSCLC; NCT03085069, ...registered March 21, 2017).
Methods
Patients who progressed during/after chemotherapy were enrolled and divided into four cohorts based on PD-L1 tumor proportion score (TPS). Patients with
EGFR
/
ALK
alterations and PD-L1 TPS ≥ 50% were also eligible. All enrolled patients received camrelizumab at 200 mg IV Q2W. The primary endpoint was objective response rate.
Results
A total of 146 patients were enrolled. As of data cutoff on Aug 20, 2020, the median follow-up was 29.5 months (95% CI 27.4–30.8). Objective response rate was 17.8% (95% CI 12.0–25.0) and improved with the increasing PD-L1 TPS (TPS < 1%, 12.2% 95% CI 5.7–21.8; ≥ 1–< 25%, 19.4% 95% CI 7.5–37.5; ≥ 25–< 50%, 36.4% 95% CI 10.9–69.2; ≥ 50%, 23.3% 95% CI 9.9–42.3). No response was observed in the five patients harboring
EGFR
mutations. Median progression-free survival was 3.2 months (95% CI 2.0–3.4), and patients with positive PD-L1 TPS had longer progression-free survival. Median overall survival was 14.8 months (95% CI 10.2–18.7). Treatment-related adverse events (TRAEs) of any grade occurred in 87.7% of patients, and 21.2% had grade ≥ 3 TRAEs.
Conclusion
Camrelizumab showed improved efficacy compared with historical data of the second-line chemotherapy in pre-treated advanced/metastatic NSCLC. Patients with positive PD-L1 expression derived greater benefit from camrelizumab. Camrelizumab has a manageable safety profile.
Purpose
To assess associations between the amount of physical activity and the prevalence of ocular diseases.
Methods
The participants of the population‐based Beijing Eye Study underwent a detailed ...ophthalmological examination and an interview. Physical activity was assessed in a standardized questionnaire.
Results
Out of 3468 study participants, information on their physical activity was available for 3031 (87.4%) individuals (age: 64.6 ± 9.7 years; range: 50–93 years). In multivariate analysis (regression coefficient r: 0.41), higher physical activity was associated with a lower prevalence of diabetic retinopathy p = 0.009; standardized regression coefficient beta: −0.05; non‐standardized regression coefficient B: −15.7; 95% confidence interval (CI): −27.6, −3.90 after adjusting for younger age, rural region of habitation, lower level of education, lower blood concentrations of triglycerides and low‐density lipoproteins and higher blood concentrations of high‐density lipoproteins, higher systolic blood pressure, lower body mass index and lower depression score. Other major ocular diseases such as open‐angle glaucoma (p = 0.25), angle‐closure glaucoma (p = 0.59), nuclear cataract (p = 0.78), cortical cataract (p = 0.54), posterior subcapsular cataract (p = 0.96), retinal vein occlusions (p = 0.93) and central serous choroidopathy (p = 0.39) were not statistically associated with physical activity in that model. The association between higher physical activity and prevalence of age‐related macular degeneration (p = 0.04; β: 0.04; B: 4.87; 95% CI: 0.25, 9.50) was marginally significant.
Conclusions
Higher physical activity and less sedentary lifestyle were associated with a lower prevalence of diabetic retinopathy, while the occurrence of other major ocular diseases such as any type of cataract and of glaucoma, retinal vein occlusions and central serous choroidopathy was statistically independent of physical activity or a more sedentary lifestyle.
Background: In the period of the COVID-19 pandemic, the level of college students’ physical exercise, the detection rate of negative emotions, and their correlation should attract extensive ...attention. Therefore, this study aimed to explore the correlation between college students’ physical exercise and negative emotions. Methods: Data were collected via a web-based cross-sectional survey. A questionnaire survey was conducted among 3118 college students from five universities in Shanghai in March 2022. In addition to sociodemographic information, measures included Physical Activity Rating Scale (PARS-3) and Depression Anxiety Stress Scale (DASS). The chi-squared test and logistic regression were used to analyze the differences and test the relative risk of negative emotions caused by different amounts of physical exercise. Results: Most students (66.1%) performed a small amount of physical exercise. Male students’ physical-exercise level was higher than female students’, and the detection rate of negative emotions was lower than that of female students. Moderate and low physical-exercise levels were associated with a higher risk of depression (beta of 0.289 and 0.345, respectively) and anxiety (beta of 0.301 and 0.418) symptoms than high physical-exercise level. Conclusions: The anxiety symptoms of college students were significant during the COVID-19 pandemic period. The physical-exercise behavior of college students was closely related to negative emotions, and the weakening of physical-exercise behavior was one of the factors that induced negative emotions in college students.
Hepatic steatosis reflects the miRNA-related pathological disorder with triglyceride accumulation and lipid peroxidation, which leads to nonalcoholic steatohepatitis, liver fibrosis/cirrhosis, and ...even hepatocellular carcinoma. Circular RNA (circRNA)/miRNA interaction reveals a novel layer of epigenetic regulation, yet the miRNA-targeting circRNA remains uncertain in hepatic steatosis. Here, we uncover circRNA_0046367 to be endogenous modulator of miR-34a that underlies hepatic steatosis. In contrast to its expression loss during the hepatocellular steatosis in vivo and in vitro, circRNA_0046367 normalization abolished miR-34a’s inhibitory effect on peroxisome proliferator-activated receptor α (PPARα) via blocking the miRNA/mRNA interaction with miRNA response elements (MREs). PPARα restoration led to the transcriptional activation of genes associated with lipid metabolism, including carnitine palmitoyltransferase 2 (CPT2) and acyl-CoA binding domain containing 3 (ACBD3), and then resulted in the steatosis resolution. Hepatotoxicity of steatosis-related lipid peroxidation, being characterized by mitochondrial dysfunction, growth arrest, and apoptosis, is resultantly prevented after the circRNA_0046367 administration. These findings indicate a circRNA_0046367/miR-34a/PPARα regulatory system underlying hepatic steatosis. Normalized expression of circRNA_0046367 may ameliorate the lipoxidative stress on the basis of steatosis attenuation. circRNA_0046367, therefore, is suggested to be potential approach to the therapy of lipid peroxidative damage.
Background
Self-efficacy is an important component of the mental well-being of college students. This study aimed to evaluate the development and the correlation between physical fitness (PF), ...exercise behavior, and self-efficacy in college students. To examine whether PF in individual college students can predict self-efficacy, and whether exercise behavior mediates this relationship.
Methods
This was an observational study of 1923 randomly selected college students (50.5% girl). Measures included the Physical Activity Rating Scale, the Self-Efficacy Scale, and PF testing.
Results
Self-efficacy was weakly correlated to both PF (
r
= 0.240) and exercise behavior (
r
= 0.248). In regression analysis, PF explained 24.7% of the variance in self-efficacy, increasing to 29.4% when exercise behavior was considered. Therefore, the predictive effect of PF on self-efficacy is partially realized through healthy exercise behavior.
Conclusion
Physical fitness can predict self-efficacy among college students, with exercise behavior being an important mediation of this relationship. Strategies to improve positive exercise behaviors and PF could improve students’ self-efficacy and overall mental health.
Strains of
, thought to play vital roles in the environment for their high enzyme production capacity during decomposition of polysaccharides, are ubiquitous in hypersaline environments. A ...Gram-negative, non-spore forming, gliding, aerobic bacterial strain, designated LN3S3
, was isolated from alkaline saline soil sampled in Tumd Right Banner, Inner Mongolia, northern PR China. Strain LN3S3
grew at 10-40 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 8.0) and with 0-12.5 % NaCl (optimum, 2.0 %). A phylogenetic tree based on the 16S rRNA gene sequences showed that strain LN3S3
clustered with
JL3085
and
MEBiC08714
, sharing 97.0, 96.7 and <96.50 % of 16S rRNA gene sequence similarities to
JL3085
,
MEBiC08714
and all other type strains. MK-7 was the major respiratory quinone, while phosphatidylethanolamine, two unidentified phospholipids, an unidentified aminophospholipid, an unidentified lipid and two unidentified aminolipids were the major polar lipids. Its major cellular fatty acids were iso-C
, anteiso-C
and summed feature 3 (C
ω7
and/or C
ω6
). The genome consisted of a circular 5 550 304 bp long chromosome with a DNA G
C content of 44.0 mol%. The average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values of strain LN3S3
to
JL3085
and
MEBiC08714
were 82.5 and 81.5 %, 87.5 and 86.0 %, and 39.1 and 35.1 %, respectively. Based on physiological, genotypic and phylogenetic analyses, strain LN3S3
could be discriminated from its phylogenetic relatives.
sp. nov. is therefore proposed with strain LN3S3
(=CGMCC 1.17081
=KCTC 72458
) as the type strain.
PDF
