A look at the antebellum history and architecture of the little-known sugar industry of East Florida .   From the late eighteenth century to early 1836, the heart of the Florida sugar industry ...was concentrated in East Florida, between the St. Johns River and the Atlantic Ocean. Producing the sweetest sugar, molasses, and rum, at least 22 sugar plantations dotted the coastline by the 1830s. This industry brought prosperity to the region—employing farm hands, slaves, architects, stone masons, riverboats and their crews, shop keepers, and merchant traders. But by January 1836, Native American attacks of the Second Seminole War, intending to rid the Florida frontier of settlers, devastated the whole sugar industry.   Although sugar works again sprang up in other Florida regions just prior to the Civil War, the competition from Louisiana and the Caribbean blocked a resurgence of sugar production for the area. The sugar industry would never regain its importance in East Florida—only two of the original sugar works were ever rebuilt. Today, remains of this once thriving industry are visible in a few parks. Some are accessible but others lie hidden, slowly disintegrating and almost forgotten. Archaeological, historical, and architectural research in the last decade has returned these works to their once prominent place in Florida’s history, revealing the beauty, efficiency of design, as well as early industrial engineering. Equally important is what can be learned of the lives of those associated with the sugar works and the early plantation days along the East Florida frontier.
NER1006 is the first 32 fluid ounce (1 L) polyethylene glycol-based bowel preparation. This randomized, multicenter, colonoscopist/central reader-blinded phase 3 non-inferiority trial assessed the ...efficacy, safety, and tolerability of NER1006 versus trisulfate for bowel cleansing.
Patients undergoing colonoscopy were randomized (1:1) to receive NER1006 or trisulfate, using evening/morning split-dosing administration. Blinded central readers used the validated Harefield Cleansing Scale to evaluate 2 alternative primary endpoints: overall bowel-cleansing success and high-quality cleansing of the ascending colon/cecum. Secondary endpoints included lesion detection, Boston Bowel Preparation Scale (BBPS) assessment, and adherence. The non-inferiority margin was 10% and the significance threshold was P < .025.
Of 621 patients randomized (NER1006, n=310; trisulfate, n=311), 556 were evaluated for efficacy (NER1006, n=276; trisulfate, n=280). NER1006 achieved non-inferiority versus trisulfate for both primary endpoints of overall bowel-cleansing success (85.1% vs 85.0%; difference, 0.14%; one-sided 97.5% lower confidence limit LCL, −8.15%; P = .528) and high-quality cleansing of the ascending colon/cecum (35.9% versus 29.3%; difference, 6.58%; LCL, −1.69%; P = .059). BBPS assessments supported overall bowel-cleansing success rates. Lesion detection rates were similar between the groups. The percentage of patients with treatment-related adverse events was 14.9% with NER1006 and 9.4% with trisulfate. Both bowel preparations showed similar overall tolerability and safety profiles. Adherence was very high in both arms.
With evening/morning split dosing, NER1006 was as effective as trisulfate for overall bowel and right-sided colon cleansing. Adverse event rates were slightly higher with NER1006 than trisulfate, but did not compromise tolerability, adherence, or efficacy. (Clinical trial registration number: NCT02254486.)
Display omitted
Summary
It is hypothesized that the virulence of phytopathogenic fungi is mediated through the secretion of small effector proteins that interfere with the defence responses of the host plant. In ...Fusarium oxysporum, one family of effectors, the Secreted In Xylem (SIX) genes, has been identified. We sought to characterize the diversity and evolution of the SIX genes in the banana‐infecting lineages of F. oxysporum f. sp. cubense (Foc). Whole‐genome sequencing data were generated for the 23 genetic lineages of Foc, which were subsequently queried for the 14 known SIX genes (SIX1–SIX14). The sequences of the identified SIX genes were confirmed in a larger collection of Foc isolates. Genealogies were generated for each of the SIX genes identified in Foc to further investigate the evolution of the SIX genes in Foc. Within Foc, variation of the SIX gene profile, including the presence of specific SIX homologues, correlated with the pathogenic race structure of Foc. Furthermore, the topologies of the SIX gene trees were discordant with the topology of an infraspecies phylogeny inferred from EF‐1α/RPB1/RPB2 (translation elongation factor‐1α/RNA polymerase II subunit I/RNA polymerase II subunit II). A series of topological constraint models provided strong evidence for the horizontal transmission of SIX genes in Foc. The horizontal inheritance of pathogenicity genes in Foc counters previous assumptions that convergent evolution has driven the polyphyletic phylogeny of Foc. This work has significant implications for the management of Foc, including the improvement of diagnostics and breeding programmes.
PDF
