There is increasing evidence that deep sequencing-based T cell repertoire can sever as a biomarker of immune response in cancer patients; however, the characteristics of T cell repertoire including ...diversity and similarity, as well as its prognostic significance in patients with cervical cancer (CC) remain unknown. In this study, we applied a high throughput T cell receptor (TCR) sequencing method to characterize the T cell repertoires of peripheral blood samples from 25 CC patients, 30 cervical intraepithelial neoplasia (CIN) patients and 20 healthy women for understanding the immune alterations during the cervix carcinogenesis. In addition, we also explored the signatures of TCR repertoires in the cervical tumor tissues and paired sentinel lymph nodes from 16 CC patients and their potential value in predicting the prognosis of patients. Our results revealed that the diversity of circulating TCR repertoire gradually decreased during the cervix carcinogenesis and progression, but the circulating TCR repertoires in CC patients were more similar to CIN patients than healthy women. Interestingly, several clonotypes uniquely detected in CC patients tended to share similar CDR3 motifs, which differed from those observed in CIN patients. In addition, the TCR repertoire diversity in sentinel lymphatic nodes from CC patients was higher than in tumor tissues. More importantly, less clonotypes in TCR repertoire of sentinel lymphatic node was associated with the poor prognosis of the patients. Overall, our findings suggested that TCR repertoire might be a potential indicator of immune monitoring and a biomarker for predicting the prognosis of CC patients. Although functional studies of T cell populations are clearly required, this study have expanded our understanding of T cell immunity during the development of CC and provided an experimental basis for further studies on its pathogenesis and immunotherapy.
Trace residues of organochlorine pesticides (OCPs) in estuarine surface sediments were investigated at three protected wetlands in southern Taiwan using high‐resolution gas ...chromatography/high‐resolution mass spectrometry (HRGC/HRMS) and gas chromatography‐electron capture detector (GC‐ECD). This study facilitates the development of new strategies for investigating OCPs, particularly at background levels. Linear relationships were obtained between total OCP concentrations (∑OCP), as analyzed by HRGC/HRMS, and the sediment's total organic carbon (TOC) and water content. It contrasted with the results acquired by GC‐ECD, where no significant relationship was found. GC‐ECD is a rugged option for daily routine practice, particularly in cases of patterns yielded by GC‐ECD is clear; the HRGC/HRMS method provides more reliable qualitative and quantitative capabilities and is highly recommended for studying the fate of OCPs and carrying out risk assessments. The average ∑OCP of these three wetlands by HRGC/HRMS was found in a range of 0.214 to 1.049 ng/g dry weight. The highest OCP level might be attributed to associated irrigation systems receiving massive discharges of domestic sewage from an urban area upstream of the wetland. The ratio of dichlorodiphenyltrichloroethane (DDT) to its metabolites indicated that the DDT residue in these areas was from aged input. According to sediment quality guidelines, adverse ecotoxicological effects of OCPs upon sediments were not expected in these protected wetlands.
Organochlorine pesticide (OCP) residues in estuarine surface sediments of protected wetlands in southern Taiwan were investigated using high‐resolution gas chromatography/high‐resolution mass spectrometry (HRGC/HRMS) and gas chromatography‐electron capture detector (GC‐ECD). This study suggests that GC‐ECD is a rugged option for daily routine practice. On the other hand, HRGC/HRMS provides a more reliable quantitative capability for a background‐level survey and risk assessment.
Given the importance of both the CF3 group and the alkyne moiety in synthetic/medicinal chemistry, we report here the first example of efficient synthesis of 2-pyrazolines with a CF3- and ...alkyne-substituted quaternary carbon center. This methodology has the advantages of high functional group compatibility, the avoidance of base and open-flask conditions, easily available and easy to handle reagent, and broad substrate scope. Notably, this protocol allows for the late-stage functionalization of biologically active molecules and the gram-scale synthesis.
At different observation intervals of 1, 5, and 10 days during a trial period of 30 days, the mortality rates of
were 100, 95, and 55%, and the fecundity rates were 0.55, 8.45, and 19.35 eggs/female, ...respectively. At temperatures of 18, 21, 24, and 27 °C, the development time of the immature stage of
was significantly shortened with increasing temperature. Furthermore, the lower developmental threshold (
) and thermal summation (
) of the immature stage were 8.91 °C and 485.44 degree-days, respectively. The greatest longevity of female and male adults reached 115.77 and 26.50 days, respectively, at 18 °C. The highest fecundity was 29.00 eggs/female at 24 °C. The population parameters of
were analyzed on the basis of the age-stage, two-sex life table theory. According to the data, the parameters were significantly affected by temperature. The highest net reproductive rate (
) was 13.32 eggs/individual at 24 °C. The highest intrinsic rate of increase (
) and finite rate of increase (
) were calculated as 0.0401 and 1.0409 day
, respectively, at a temperature of 27 °C. The shortest mean generation time (
) was 51.34 days at 27 °C. Overall, we provide a discussion on comprehensive biological information regarding
, thus providing basic knowledge for further research on this pest.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
Injury to the internal carotid artery (ICA) is the most critical complication of endoscopic endonasal skull base surgery. Packing with a crushed muscle graft at the injury site has been an effective ...management technique to control bleeding without ICA sacrifice. Obtaining the muscle graft has typically required access to another surgical site, however. To address this concern, the authors investigated the application of an endonasally harvested longus capitis muscle patch for the management of ICA injury.
One colored silicone-injected anatomical specimen was dissected to replicate the surgical access to the nasopharynx and the stepwise dissection of the longus capitis muscle in the nasopharynx. Two representative cases were selected to illustrate the application of the longus capitis muscle patch and the relevance of clinical considerations.
A suitable muscle graft from the longus capitis muscle could be easily and quickly harvested during endoscopic endonasal skull base surgery. In the illustrative cases, the longus capitis muscle patch was successfully used for secondary prevention of pseudoaneurysm formation following primary bleeding control on the site of ICA injury.
Nasopharyngeal harvest of a longus capitis muscle graft is a safe and practical method to manage ICA injury during endoscopic endonasal surgery.
Background Application of autologous adipose-derived stem cells (ASC) for diabetic chronic wounds has become an emerging treatment option. However, ASCs from diabetic individuals showed impaired cell ...function and suboptimal wound healing effects. We proposed that adopting a low-glucose level in the culture medium for diabetic ASCs may restore their pro-healing capabilities. Methods ASCs from diabetic humans and mice were retrieved and cultured in high-glucose (HG, 4.5 g/L) or low-glucose (LG, 1.0 g/L) conditions. Cell characteristics and functions were investigated in vitro. Moreover, we applied diabetic murine ASCs cultured in HG or LG condition to a wound healing model in diabetic mice to compare their healing capabilities in vivo. Results Human ASCs exhibited decreased cell proliferation and migration with enhanced senescence when cultured in HG condition in vitro. Similar findings were noted in ASCs derived from diabetic mice. The inferior cellular functions could be partially recovered when they were cultured in LG condition. In the animal study, wounds healed faster when treated with HG- or LG-cultured diabetic ASCs relative to the control group. Moreover, higher collagen density, more angiogenesis and cellular retention of applied ASCs were found in wound tissues treated with diabetic ASCs cultured in LG condition. Conclusions In line with the literature, our study showed that a diabetic milieu exerts an adverse effect on ASCs. Adopting LG culture condition is a simple and effective approach to enhance the wound healing capabilities of diabetic ASCs, which is valuable for the clinical application of autologous ASCs from diabetic patients. Keywords: Stem cell culture, Diabetes, Adipose-derived stem cell, Stem cell transplantation, Tissue regeneration
: The efficacy of remdesivir in reducing disease severity among COVID-19-infected patients has been established, but concerns have emerged regarding the potential side effects of bradycardia. The aim ...of this study was to investigate the association between remdesivir-induced bradycardia and mortality, while also identifying the related risk factors.
: The PubMed/Medline, Cochrane Central and ClinicalTrials.gov databases were searched. Randomized controlled trials and prospective or retrospective cohort studies were included (through 14 July 2023). The random-effects model was implemented using Comprehensive Meta-Analysis software version 3.0 to examine the outcomes.
: A total of 12 prospective or retrospective studies involving 7674 patients were analyzed. The primary outcomes revealed a significant association between remdesivir administration and bradycardia development (Odds ratio = 2.556, 95% CI = 2.049-3.188,
< 0.001). However, no statistically significant increase in the mortality rate was observed among patients with bradycardia during remdesivir treatment (Odds ratio = 0.872, 95% CI = 0.483-1.576,
= 0.651). The secondary outcome demonstrated a significant association between chronic kidney disease (CKD) and remdesivir-induced bradycardia (OR: 1.251, 95% CI: 1.003-1.561,
= 0.047). Moreover, patients with obesity (OR = 1.347, 95% CI = 1.098-1.652,
= 0.004) were more likely to experience remdesivir-induced bradycardia.
: Although a higher risk of bradycardia occurred during remdesivir treatment, the occurrence of remdesivir-induced bradycardia did not lead to higher mortality. Our study also identified patients with obesity and CKD as high-risk subgroups for experiencing bradycardia during remdesivir treatment.
The development of gas sensors with high selectivity to volatile ketones at low temperature has recently been a hot topic. However, the reported good response to butanone is usually achieved above ...200 °C. Herein, based on the conception of biomimicry, we chose waste phoenix tree leaves (PTL) as template and simply immersed them in iron nitrate solution. Subsequently, biomorphic Fe2O3 nanosheets were controllably replicated by calcining immersed PTL precursor in air. Thereinto, Fe2O3-600 nanosheets obtained by calcining precursor at 600 °C are cross-linked by nanoparticles and have broad pore structure and large surface area. Such unique microstructure induced by bio-template imprinting not only facilitates the fast gas diffusion, but also exposes more active sites to accelerate gas adsorption and its chemical reaction with adsorbed oxygen species. In this sense, it firstly realizes the highly selective detection of Fe2O3-based material to trace butanone. At 170 °C, Fe2O3-600 sensor shows high sensing response (S = 25.8) and short response time (Tres = 5 s) to 100 ppm butanone vapor. This sensor still possesses small detection limit (100 ppb), good reversibility and anti-humidity. In addition, we explored the sensing mechanism of Fe2O3-600 nanosheets to butanone by O2-TPD, XPS and GC-MS techniques.
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•Fe2O3 nanosheets were simply synthesized using phoenix tree leaves as bio-template.•Fe2O3-600 sensor shows high sensing ability to butanone at low working temperature.•The low-cost PTL-template method provides a new vision for preparing metal oxides.
To study the basic mechanical behavior and the reloading reinforcement characteristics of fractured coal, conventional triaxial loading tests with different fissure angle were first carried out. On ...this basis, conventional triaxial loading and unloading tests were conducted to investigate the reloading reinforcement characteristics of fractured coal. The results reveal that when the fissure angle was small, the stress-strain curve exhibited the multi-peak phenomena. As the fissure angle increased, the stress drop phenomenon in the peak region was weakened. With the increase of the fissure angle, the peak stress of the specimens increased and then decreased, while the elastic modulus showed an overall increasing trend, demonstrating the controlling effect of the crack angle. Meanwhile, the cyclic loading exhibited a certain enhancement effect on the strength of the fractured coals when the specimens was unloaded near the crack closure stress. The findings can provide a better understanding of the failure mechanism and reloading reinforcement characteristics of fractured coal.
The T-cell immune responses in nasopharyngeal carcinoma patients have been extensively investigated recently for designing adoptive immunotherapy or immune checkpoint blockade therapy. However, the ...distribution characteristics of T cells associated with NPC pathogenesis are largely unknown. We performed deep sequencing for TCR repertoire profiling on matched tumor/adjacent normal tissue from 15 NPC patients and peripheral blood from 39 NPC patients, 39 patients with other nasopharyngeal diseases, and 33 healthy controls. We found that a lower diversity of TCR repertoire in tumors than paired tissues or a low similarity between the paired tissues was associated with a poor prognosis in NPC. A more diverse TCR repertoire was identified in the peripheral blood of NPC patients relative to the controls; this was related to a significant decrease in the proportion of high-frequency TCR clones in NPC. Higher diversity in peripheral blood of NPC patients was associated with a worse prognosis. Due to the peculiarity of the Vβ gene usage patterns in the peripheral blood of NPC patients, 15 Vβ genes were selected to distinguish NPC patients from controls by the least absolute shrinkage and selection operator analysis. We identified 11 clonotypes shared by tumors and peripheral blood samples from different NPC patients, defined as “NPC-associated” that might have value in adoptive immunotherapy. In conclusion, we here report the systematic and overall characteristics of the TCR repertoire in tumors, adjacent normal tissues, and peripheral blood of NPC patients. The data obtained may be relevant to future clinical studies in the setting of immunotherapy for NPC patients.
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