Biosurfactants (BS) are amphipathic compounds produced by diverse groups of microorganisms exhibiting various biological activities. The current study aimed to assess antimicrobial, anti-adhesive and ...anti-biofilm activities of BS isolated from lactic acid bacteria (LAB), including Pediococcus acidilactici and Lactobacillus plantarum against Staphylococcus aureus CMCC 26003 in vitro. Cell-bound BS from both Pediococcus acidilactici and Lactobacillus plantarum were extracted, and their surface activities were evaluated by oil spreading assay. As quantified by crystal violet method, BS inhibited adhesion and biofilm formation of Staphylococcus aureus in a dose-dependent manner. The above findings were further supported by results of scanning electron microscopy. These two kinds of BS affect expressions of biofilm-related genes (cidA, icaA, dltB, agrA, sortaseA and sarA) and interfere with the release of signaling molecules (AI-2) in quorum sensing systems. Biological activities observed for BS produced by tested LAB suggest prospects for their use against Staphylococcus aureus biofilm-related infections.
•Crude BS produced by L. plantarum and P. acidilactici has good anti-adhesion and anti-biofilm activity against S. aureus.•The expression of genes related to biofilm formation (cidA, icaA, dltB, agrA, sortaseA and sarA) is affected by crude BS.•Crude BS interfere with the release of signaling molecules (AI-2) in quorum sensing systems.•BS can be used as special anti-bacterial biofilm biologicals, but has minimal effect on bacterial growth.
Self-serving bias suggests that people tend to attribute success to internal factors and attribute failure to external factors (Bradley, J Pers Soc Psychol 36:56–71,1978; Miller and Ross, Psychol ...Bull 82:213–225,1975). However, the results of the attribution of failure are not always consistent. Some studies have found that people attribute failure to external factors (Snyder, Stephan, & Rosenfileld, 1976) and others suggest that people attribute failure to internal factors (Ross et al., J Pers Soc Psychol 29:609–618,1974). I tested self-serving bias in two different contexts in mainland China: in one, test results were public (students had access to each other’s test results) and in the other, test results were private (students only had access to his/her own results). When a context triggers individuals to compare themselves to others, individuals may alter their attribution of failure in order to preserve their self-image and selfesteem. Data were analyzed by repeated measure ANOVA, and the results show that in a public context people tend to attribute failure more to external factors than to themselves. Also, results suggest that people attribute failure less to themselves in a public context than in a private context.
Climate change has substantial impacts on regional hydrology in the major river basins. To figure out such latent hydrological impacts of changing climate, more reliable hydrological simulations are ...imperative. In this study, we evaluated the impacts of climate change on hydrological regime in the Upper Yangtze River Basin based on four downscaled and bias-corrected Global Climate Model outputs from Coupled Model Intercomparison Project Phase 5 under four Representative Concentration Pathways (RCP2.6, RCP4.5, RCP6.0, and RCP8.5) driving three hydrological models. Two model evaluation approaches were applied: simple and comprehensive. The comprehensive approach was used to evaluate models in the historical period, optimizing objective function at four gauges, and hydrological models were weighted for impact assessment based on their performance. In such a way, projected streamflow time series are obtained under different emission scenarios. Results show that the annual average discharge is projected to increase by 4.1–10.5% under the RCP scenarios at the end of twenty-first century relative to the reference period (1970–1999). Moreover, the high flow is projected to increase and the low flow to decrease indicating a higher probability of flood and drought occurrence in the basin. The severity of floods and droughts may increase. In comparison with the simple one-site model evaluation approach, the comprehensive method reveals that the anticipated extreme flow events would be less severe, and annual mean discharge slightly lower. The projected results imply that application of the comprehensive model evaluation approach could narrow the simulated spreads of projections significantly, and might provide more credible results.
Hexaploid bread wheat (Triticum aestivum L., genome BBAADD) is generally more salt tolerant than its tetraploid wheat progenitor (Triticum turgidum L.). However, little is known about the ...physiological basis of this trait or about the relative contributions of allohexaploidization and subsequent evolutionary genetic changes on the trait development. Here, we compared the salt tolerance of a synthetic allohexaploid wheat (neo-6 x) with its tetraploid (T. turgidum ; BBAA) and diploid (Aegilops tauschii; DD) parents, as well as a natural hexaploid bread wheat (nat-6 x). We studied 92 morphophysiological traits and analyzed homeologous gene expression of a major salt-tolerance gene High-Affinity K ⁺ Transporter 1;5 (HKT1;5). We observed that under salt stress, neo-6 x exhibited higher fitness than both of its parental genotypes due to inheritance of favorable traits like higher germination rate from the 4 x parent and the stronger root Na ⁺ retention capacity from the 2 x parent. Moreover, expression of the D-subgenome HKT1;5 homeolog, which is responsible for Na ⁺ removal from the xylem vessels, showed an immediate transcriptional reprogramming following allohexaploidization, i.e., from constitutive high basal expression in Ae. tauschii (2 x) to salt-induced expression in neo-6 x . This phenomenon was also witnessed in the nat-6 x . An integrated analysis of 92 traits showed that, under salt-stress conditions, neo-6 x resembled more closely the 2 x than the 4 x parent, suggesting that the salt stress induces enhanced expressivity of the D-subgenome homeologs in the synthetic hexaploid wheat. Collectively, the results suggest that condition-dependent functionalization of the subgenomes might have contributed to the wide-ranging adaptability of natural hexaploid wheat.
In service contexts, leaders' efforts to maintain and enhance employees' service quality are vital to organizational performance. However, few studies have investigated the mechanisms underlying the ...relationship between transformational leadership (TFL) and employees' service quality across levels. Based on the theory of planned behavior, this study investigated the multilevel impacts of TFL on employees' service quality and the multilevel mechanisms mediating this relationship. Data were collected from 208 managers and 1,431 employees at 223 branches (chain restaurants) of a large catering corporation in southern China. The results showed that stronger TFL significantly improved employees' service quality at both individual and branch level. Affective commitment and psychological empowerment partially mediated the relationship between individual-level TFL and employees' service quality. Branch-level service climate fully mediated the relationship between branch-level TFL and employees' service quality.
This study aimed to assess the performance of 35 global climate models included in NEX-GDDP-CMIP6, derived from downscaling CMIP6 data to high spatial (25 km) and temporal (daily) resolutions, in ...reproducing extreme precipitation events over the Huai River Basin. Eight widely used extreme precipitation indices were employed to quantitatively describe the models’ capability of simulation. Results indicate that the majority of models can reasonably capture trends, with UKESM1-0-LL performing the best among all considered models. All models demonstrate high accuracy in simulating climatological means, especially for the total precipitation (PRCPTOT), displaying a spatial correlation coefficient exceeding 0.8 when compared to the observed data. NorESM2-MM and MRI-ESM2-0 can accurately simulate the frequency and intensity of extreme precipitation, respectively. In general, UKESM1-0-LL, CESM2, MIROC6, MRI-ESM2-0, CMCC-CM2-SR5, and MPI-ESM-2-LR exhibit superior simulation capabilities in terms of capturing both the trends and climatology of extreme precipitation. The aforementioned findings provide guidance for future studies on the regional impacts of climate change using NEX model data, and therefore hold great importance in comprehending the regional impacts of, and the adaptability to, climate change, as well as the development of adaptation strategies.
The 12-oxo-phytodienoic acid reductases (OPRs), which belong to the old yellow enzyme (OYE) family, are flavin mononucleotide (FMN)-dependent oxidoreductases with critical functions in plants. ...Despite the clear characteristics of growth and development, as well as the defense responses in
, tomato, rice, and maize, the potential roles of OPRs in wheat are not fully understood. Here, forty-eight putative
genes were found and classified into five subfamilies, with 6 in sub. I, 4 in sub. II, 33 in sub. III, 3 in sub. IV, and 2 in sub. V. Similar gene structures and conserved protein motifs of TaOPRs in wheat were identified in the same subfamilies. An analysis of cis-acting elements in promoters revealed that the functions of OPRs in wheat were mostly related to growth, development, hormones, biotic, and abiotic stresses. A total of 14 wheat
genes were identified as tandem duplicated genes, while 37
genes were segmentally duplicated genes. The expression patterns of TaOPRs were tissue- and stress-specific, and the expression of TaOPRs could be regulated or induced by phytohormones and various stresses. Therefore, there were multiple wheat
genes, classified into five subfamilies, with functional diversification and specific expression patterns, and to our knowledge, this was the first study to systematically investigate the wheat
gene family. The findings not only provide a scientific foundation for the comprehensive understanding of the wheat
gene family, but could also be helpful for screening more candidate genes and breeding new varieties of wheat, with a high yield and stress resistance.
Enterotoxigenic Escherichia coli (ETEC) is a significant contributor to diarrhea. To determine whether ETEC-catecholamine hormone interactions contribute to the development of diarrhea, we tested the ...effects of catecholamine hormones acting on ETEC in vitro. The results showed that in the presence of norepinephrine (NE) and epinephrine (Epi), the growth of 9 out of 10 ETEC isolates was promoted, the MICs of more than 60% of the isolates to 6 antibiotics significantly increased, and the biofilm formation ability of 10 ETEC isolates was also promoted. In addition, NE and Epi also significantly upregulated the expression of the virulence genes feaG, estA, estB, and elt. Transcriptome analysis revealed that the expression of 290 genes was affected by NE. These data demonstrated that catecholamine hormones may augment the diarrhea caused by ETEC.
Extensive studies have revealed the function and mechanism of lncRNAs in development and differentiation, but the majority have focused on those lncRNAs adjacent to protein-coding genes. In contrast, ...lncRNAs located in gene deserts are rarely explored. Here, we utilize multiple differentiation systems to dissect the role of a desert lncRNA, HIDEN (human IMP1-associated "desert" definitive endoderm lncRNA), in definitive endoderm differentiation from human pluripotent stem cells.
We show that desert lncRNAs are highly expressed with cell-stage-specific patterns and conserved subcellular localization during stem cell differentiation. We then focus on the desert lncRNA HIDEN which is upregulated and plays a vital role during human endoderm differentiation. We find depletion of HIDEN by either shRNA or promoter deletion significantly impairs human endoderm differentiation. HIDEN functionally interacts with RNA-binding protein IMP1 (IGF2BP1), which is also required for endoderm differentiation. Loss of HIDEN or IMP1 results in reduced WNT activity, and WNT agonist rescues endoderm differentiation deficiency caused by the depletion of HIDEN or IMP1. Moreover, HIDEN depletion reduces the interaction between IMP1 protein and FZD5 mRNA and causes the destabilization of FZD5 mRNA, which is a WNT receptor and necessary for definitive endoderm differentiation.
These data suggest that desert lncRNA HIDEN facilitates the interaction between IMP1 and FZD5 mRNA, stabilizing FZD5 mRNA which activates WNT signaling and promotes human definitive endoderm differentiation.
To understand the molecular changes taking place during the early grain development in common wheat, we profiled transcriptome and proteome of two cultivars, “P271” and “Chinese Spring” (CS) with ...large and small grains, respectively. More than 85,000 genes and 7500 proteins were identified to express during early grain development in two wheat cultivars. We observed enrichment in the number of genes falling in the functional categories—carbohydrate metabolism, amino acid metabolism, lipid metabolism, and cofactor as well as vitamin metabolism with progression in grain development, which indicates towards the importance of these metabolic pathways during grain maturation. Many genes showed inconsistency between transcription and translation, which suggested a role of post-transcriptional events that determine the fate of nascent transcript/protein, in the early grain development. In silico localization of differentially expressed genes/proteins between CS and P271 to wheat chromosomes, exhibited a biased genomic distribution with chromosomes 1A, 4B, and 5B contributing primarily to it. These results corroborated the earlier findings, where chromosomes 1A, 4B, and 5B were reported to harbor genes/QTLs for yield contributing traits such as grain length and thickness. Collectively, this study reveals the molecular changes taking place during early grain development, through light on the regulation of these processes, and allows identification of the gene candidates contributing to the contrasting grain characteristics of CS and P721. This information has implications in the future wheat breeding for the enhanced grain yield.
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