The accurate calibration of photomultiplier tubes is critical in a wide variety of applications for which it is necessary to know the absolute number of detected photons or precisely determine the ...resolution of the signal. Conventional calibration methods rely on fitting the photomultiplier response to a low intensity light source with analytical approximations to the single photoelectron distribution. We show that this approach often leads to biased estimates due to an inability to model the full distribution accurately, especially at low charge values. We present a simple statistical method to extract the relevant single photoelectron calibration parameters (first two central moments) without making any assumptions about the underlying single photoelectron distribution. We illustrate the use of this method through the calibration of a Hamamatsu R11410 photomultiplier tube and study the accuracy and precision of the method using Monte Carlo simulations. The method is found to have significantly reduced bias compared to conventional methods and works under a wide range of light intensities, making it suitable for the simultaneous calibration of large arrays of photomultiplier tubes where uniform illumination may not be possible.
Consumer perception of organic cow milk is associated with the assumption that organic milk differs from conventionally produced milk. The value associated with this difference justifies the premium ...retail price for organic milk. It includes the perceptions that organic dairy farming is kinder to the environment, animals, and people; that organic milk products are produced without the use of antibiotics, added hormones, synthetic chemicals, and genetic modification; and that they may have potential benefits for human health. Controlled studies investigating whether differences exist between organic and conventionally produced milk have so far been largely equivocal due principally to the complexity of the research question and the number of factors that can influence milk composition. A main complication is that farming practices and their effects differ depending on country, region, year, and season between and within organic and conventional systems. Factors influencing milk composition (e.g., diet, breed, and stage of lactation) have been studied individually, whereas interactions between multiple factors have been largely ignored. Studies that fail to consider that factors other than the farming system (organic vs. conventional) could have caused or contributed to the reported differences in milk composition make it impossible to determine whether a system-related difference exists between organic and conventional milk. Milk fatty acid composition has been a central research area when comparing organic and conventional milk largely because the milk fatty acid profile responds rapidly and is very sensitive to changes in diet. Consequently, the effect of farming practices (high input vs. low input) rather than farming system (organic vs. conventional) determines milk fatty acid profile, and similar results are seen between low-input organic and low-input conventional milks. This confounds our ability to develop an analytical method to distinguish organic from conventionally produced milk and provide product verification. Lack of research on interactions between several influential factors and differences in trial complexity and consistency between studies (e.g., sampling period, sample size, reporting of experimental conditions) complicate data interpretation and prevent us from making unequivocal conclusions. The first part of this review provides a detailed summary of individual factors known to influence milk composition. The second part presents an overview of studies that have compared organic and conventional milk and discusses their findings within the framework of the various factors presented in part one.
DARk matter WImp search with liquid xenoN (DARWIN) will be an experiment for the direct detection of dark matter using a multi-ton liquid xenon time projection chamber at its core. Its primary goal ...will be to explore the experimentally accessible parameter space for Weakly Interacting Massive Particles (WIMPs) in a wide mass-range, until neutrino interactions with the target become an irreducible background. The prompt scintillation light and the charge signals induced by particle interactions in the xenon will be observed by VUV sensitive, ultra-low background photosensors. Besides its excellent sensitivity to WIMPs above a mass of 5 GeV/c2, such a detector with its large mass, low-energy threshold and ultra-low background level will also be sensitive to other rare interactions. It will search for solar axions, galactic axion-like particles and the neutrinoless double-beta decay of 136-Xe, as well as measure the low-energy solar neutrino flux with <1% precision, observe coherent neutrino-nucleus interactions, and detect galactic supernovae. We present the concept of the DARWIN detector and discuss its physics reach, the main sources of backgrounds and the ongoing detector design and R&D efforts.
The current study was carried out to measure the basal ileal endogenous amino acid (EAA) flow in male broilers (Ross 308) at different ages (d 7, 14, 21, 28, 35, and 42), following the feeding of a ...nitrogen-free diet. Titanium dioxide (5 g/kg) was included as an indigestible marker. The nitrogen-free diet was offered for four days prior to ileal digesta collection to 6 replicate cages housing 14 (d 3–7), 12 (d 10–14), 10 (d 17–21), 8 (d 24–28), 8 (d 31–35), and 6 (d 38–42) birds per cage. The basal EAA flow was calculated as g/kg DM intake. The amino acid (AA) profile of endogenous protein, expressed as g/100 g protein, was also calculated. The basal endogenous flow of nitrogen and all individual and total AA decreased quadratically (P < 0.05 to 0.001), with flows being higher on d 7, then decreasing on d 14, plateauing until d 35 and decreasing further on d 42. The concentrations of Trp, Cys, and Gly in the endogenous protein increased linearly (P < 0.01 to 0.001) with advancing age, whereas a linear decrease (P < 0.001) was noted for Lys. A quadratic influence (P < 0.05 to 0.001) was observed for the concentrations of Ile, Leu, Met, Val, and Asp. These changes in the endogenous protein profile may be attributed to variations in the contribution of endogenous sources with age but delineating the exact contribution of different sources is complicated. Overall, the current findings suggest that the basal ileal EAA flow is influenced by broiler age and age-specific EAA flows may need to be considered to standardize the AA digestibility.
The influence of supplemental carbohydrase (Carb) and conditioning temperature (CT) on growth performance, nutrient utilization and intestinal morphometry of broilers (d 1–21) fed barley-based diets ...was examined in a 2 × 3 factorial arrangement, evaluating 2 levels of Carb (0 and 150 g/tonne of feed) and three CT (60, 74, and 88°C). A total of 288, 1-day-old male broilers (8 birds/cage; 6 cages/treatment) were used. The activities of endo-1,4-β- glucanase, endo-1,3 (4)-β-glucanase and endo-1,4-β-xylanase in the Carb were 800 BGU/g, 700 BGU/g and 2,700 XU/g, respectively. On d 21, ileal digesta was collected for the determination of nutrient digestibility. There was no significant interaction between Carb and CT for any tested parameter. Supplemental Carb, regardless of CT, increased weight gain (WG; P < 0.05) and reduced feed per gain (F/G; P < 0.001) by 30 g/bird and 6.5 points, respectively. Increasing CT to 88°C reduced (P < 0.05) WG, but increased (P < 0.05) F/G compared to the diets conditioned at 60° and 74°C. Regardless of CT, Carb enhanced (P < 0.05) the digestibility of starch and AMEn by 1.15% and 32 kcal/kg, respectively. Compared to the diets conditioned at 60° and 74°C, CT at 88°C reduced (P < 0.05) digestibility of dry matter, nitrogen, phosphorus, gross energy, and AMEn. Birds fed diets conditioned at 88°C showed lower (P < 0.05) starch digestibility compared to those fed diets conditioned at 60°C. Conditioning at 88°C increased (P < 0.05) jejunal digesta viscosity by 10.2% compared to diets conditioned at 60° and 74°C. Overall, Carb supplementation improved WG, F/G, starch digestibility and AMEn in broilers fed barley-based diets, irrespective of CT applied. Conditioning barley-based diets at 88°C impaired the ability of birds to utilize nitrogen, starch, phosphorus and energy, and consequently deteriorated WG and F/G. The lack of significant interactions between Carb and CT indicated that negative impacts caused by high CT on bird performance and nutrient utilization occurred regardless of Carb enzyme supplementation. Supplemental Carb per se could not remedy the adverse effects of high CT.
Impaired intestinal microcirculation seems to play an important role in the pathogenesis of necrotizing enterocolitis (NEC). A previous study showed that a SrSO
< 30% is associated with an increased ...risk of developing of NEC. We aimed to determine the clinical usefulness of the cut off < 30% for SrSO
in predicting NEC in extremely preterm neonates.
This is a combined cohort observational study. We added a second cohort from another university hospital to the previous cohort of extremely preterm infants. SrSO
was measured for 1-2 h at days 2-6 after birth. To determine clinical usefulness we assessed sensitivity, specificity, positive and negative predictive values for mean SrSO
< 30. Odds ratio to develop NEC was assessed with generalized linear model analysis, adjusting for center.
We included 86 extremely preterm infants, median gestational age 26.3 weeks (range 23.0-27.9). Seventeen infants developed NEC. A mean SrSO
< 30% was found in 70.5% of infants who developed NEC compared to 33.3% of those who did not (p = 0.01). Positive and negative predictive values were 0.33 CI (0.24-0.44) and 0.90 CI (0.83-0.96), respectively. The odds of developing NEC were 4.5 (95% CI 1.4-14.3) times higher in infants with SrSO2 < 30% compared to those with SrSO2 ≥ 30%.
A mean SrSO
cut off ≥ 30% in extremely preterm infants between days 2-6 after birth may be useful in identifying infants who will not develop NEC.
The design of rotor blades is based on information about aerodynamic phenomena. An important one is fluid-structure interaction (FSI) which describes the interaction between a flexible object (rotor ...blade) and the surrounding fluid (wind). However, the acquisition of FSI is complex, and only a few practical concepts are known. This paper presents a measurement setup to acquire real information about the FSI of rotating wind turbines in wind tunnel experiments. The setup consists of two optical measurement systems to simultaneously record fluid (PIV system) and deformation (photogrammetry system) information in one global coordinate system. Techniques to combine both systems temporally and spatially are discussed in this paper. Furthermore, the successful application is shown by several experiments. Here, different wind conditions are applied. The experiments show that the new setup can acquire high-quality area-based information about fluid and deformation.
Thyroid eye disease (TED) is a rare disease that can lead to decreased quality of life, permanent disfigurement, and vision loss. Clinically, TED presents with exophthalmos, periorbital edema, ...extraocular muscle dysfunction, and eyelid retraction, and can lead to vision-threatening complications such as exposure to keratopathy and dysthyroid optic neuropathy (DON). Over the last several years, significant advancements have been made in the understanding of its pathophysiology as well as optimal management. Ethnic variations in the prevalence, clinical presentation, and risk of vision-threatening complications of TED are summarized, and risk factors associated with TED are discussed. Additionally, significant advances have been made in the management of TED. The management of TED traditionally included anti-inflammatory medications, orbital radiation therapy, orbital surgical decompression, and biologic therapies. Most recently, targeted therapies such as teprotumumab, an insulin-like growth factor-1 receptor antagonist, have been studied in the context of TED, with promising initial data. In this review, updates in the understanding and management of TED are presented with a focus on the international variations in presentation and management.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Thyroid eye disease (TED) is a complex disease associated with myriad clinical presentations, including facial disfigurement, vision loss, and decreased quality of life. Traditionally, steroid ...therapy and/or radiation therapy were commonly used in the treatment of active TED. While these therapies can help reduce inflammation, they often do not have a sustainable, significant long-term effect on disease outcomes, including proptosis and diplopia. Recent advances in our understanding of the pathophysiology of TED have shifted the focus of treatment toward targeted biologic therapies. Biologics have the advantage of precise immune modulation, which can have better safety profiles and greater efficacy compared to traditional approaches. For instance, the insulin-like growth factor-1 receptor (IGF-1R) has been found to be upregulated in TED patients and to colocalize with the thyroid-stimulating hormone receptor (TSHR), forming a signaling complex. Teprotumumab is an antibody targeted against IGF-1R. By inhibiting the IGF-1R/TSHR signaling pathway, teprotumumab may reduce the production of proinflammatory cytokines, hyaluronan secretion, and orbital fibroblast activation in patients with TED. Due to promising phase II and III clinical trial results, teprotumumab has become the first biologic US Food and Drug Administration (FDA)-approved for the treatment of TED. In addition, there are currently ongoing studies looking at the use of antibodies targeting the neonatal Fc receptor (FcRn) in various autoimmune diseases, including TED. FcRn functions to transport immunoglobulin G (IgG) and prevent their lysosomal degradation. By blocking the recycling of IgG, this approach may dampen the body’s immune response, in particular the pathogenic IgG implicated in some autoimmune diseases. Advances in our understanding of the pathophysiology of TED, therefore, are leading to more targeted therapeutic options, and we are entering an exciting new phase in the management of TED. This review will cover recent insights into the understanding of TED pathophysiology and novel treatment options as well as ongoing studies of new potential treatment options for TED.
Standardized ileal digestibility coefficients (SIDC) of nitrogen (N) and amino acids (AA) in wheat and sorghum at 6 different ages (d 7, 14, 21, 28, 35, and 42) of broilers were determined. Two assay ...diets were formulated to contain 93.8% of each grain as the sole source of AA in the diet. Titanium dioxide (0.5%) was added as an indigestible marker. Each assay diet was fed to 6 replicate cages housing 14 (d 7), 12 (d 14), 10 (d 21), 8 (d 28), 8 (d 35), and 6 (d 42) birds per cage for 4 d prior to ileal digesta collection. The apparent ileal digestibility coefficients (AIDC) were standardized by using the age-appropriate basal endogenous AA losses. In the case of wheat, AIDC of N and all AA increased (linear or quadratic, P < 0.05 to 0.001) with advancing age. No age effect was noticed on the SIDC of N, average of indispensable (IAA) and dispensable AA (DAA), though the average of total AA (TAA) tended (linear, P = 0.09) to increase as birds grew older. In sorghum, the AIDC of N, average of IAA and DAA were unaffected (P > 0.05) by age. The SIDC of N, average SIDC of IAA, DAA and TAA were higher at d 7, reduced at d 14 and then plateaued. Among the IAA, the SIDC of Arg, His, Ile, Leu, Lys, Thr, Val, and the SIDC of all individual DAA (except Cys) decreased with age (linear or quadratic, P < 0.05 to 0.001) with higher values at d 7. The higher SIDC values determined at d 7 were due to higher EAA losses during wk 1. The results showed that broiler age influences AA digestibility and this may need be considered in practical feed formulations. The age effect is variable depending on the grain type and specific AA.
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