To evaluate the dynamic range of tissue imaged by elastography, the mechanical behavior of breast and prostate tissue samples subject to compression loading has been investigated. A model for the ...loading was validated and used to guide the experimental design for data collection. The model allowed the use of small samples that could be considered homogeneous; this assumption was confirmed by histological analysis. The samples were tested at three strain rates to evaluate the viscoelastic nature of the material and determine the validity of modeling the tissue as an elastic material for the strain rates of interest. For loading frequencies above 1 Hz, the storage modulus accounted for over 93 percent of the complex modulus. The data show that breast fat tissue has a constant modulus over the strain range tested while the other tissues have a modulus that is dependent on the strain level. The fibrous tissue samples from the breast were found to be 1 to 2 orders of magnitude stiffer than fat tissue. Normal glandular breast tissue was found to have an elastic modulus similar to that of fat at low strain levels, but the modulus of the glandular tissue increased by an order of magnitude above fat at high strain levels. Carcinomas from the breast were stiffer than the other tissues at the higher strain level; intraductal in situ carcinomas were like fat at the low strain level and much stiffer than glandular tissue at the high strain level. Infiltrating ductal carcinomas were much stiffer than any of the other breast tissues. Normal prostate tissue has a modulus that is lower than the modulus of the prostate cancers tested. Tissue from prostate with benign prostatic hyperplasia (BPH) had modulus values significantly lower than normal tissue. There was a constant but not significant difference in the modulus of tissues taken from the anterior and posterior portions of the gland.
Sepsis is associated with a mortality rate of 30 to 50 percent and with substantial morbidity.
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The relative contributions of the inflammatory response and infection to these adverse outcomes are ...unknown.
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In animal models of sepsis, treatment with nonsteroidal antiinflammatory drugs improves survival and reduces physiologic abnormalities.
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The synthesis of prostaglandin and thromboxane has been linked with abnormalities of airway mechanics, pulmonary hypertension, hypoxemia, cardiovascular collapse, and multiple organ failure in animals and in humans with the sepsis syndrome.
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Ibuprofen has been shown to have effects on sepsis in humans, but because of their small samples (fewer . . .
Background Poor hand hygiene on college campuses can be improved by promoting handwashing behavior. This observational study was conducted to evaluate gender and race/ethnic differences in hand ...hygiene practices among college students. Methods Hand hygiene practices in college students were evaluated in 4 settings (soap and water; soap and water and visual prompts; soap and water and hand sanitizers; and soap and water, hand sanitizers, and visual prompts). The degree of hand hygiene (ie, adequate handwashing time, use of hand sanitizer, and hand-drying method) also was evaluated at various locations on campus. Results Overall, 72.9% of students washed their hands, 58.3% practiced hand hygiene (using either soap or hand sanitizer), and 26.1% washed their hands adequately. Hand sanitizer use was low when students were given the option, and paper towel was the most common hand-drying method. In general, hand hygiene practices were better in academic buildings than in the student recreation center. Visual prompts improved handwashing behavior only among students in the “other” ethnic category, but not by gender. Conclusions Handwashing is the most effective way of preventing the spread of infectious diseases, and our findings have implications for the design of effective hand hygiene education programs in college students.
Agriculture in the Central Valley of California, one of the USA’s main sources of fruits, nuts, and vegetables, is highly vulnerable to climate change impacts in the next 50 years. This ...interdisciplinary case study in Yolo County shows the urgency for building adaptation strategies to climate change. Climate change and the effects of greenhouse gas emissions are complex, and several of the county’s current crops will be less viable in 2050. The study uses a variety of methods to assemble information relevant to Yolo County’s agriculture, including literature reviews, models, geographic information system analysis, interviews with agency personnel, and a survey of farmers. Potential adaptation and mitigation responses by growers include changes in crop taxa, irrigation methods, fertilization practices, tillage practices, and land use. On a regional basis, planning must consider the vulnerability of agricultural production and the tradeoffs associated with diversified farmlands, drought, flooding of cropland, loss of habitat for wild species of concern, and urbanization.
Continuous ECG characteristics were compared using Student t tests and dichotomous variables using chi-square tests. Because of differences in age, a secondary analysis with only HCM patients under ...40 years of age was performed. Compared to athletes, patients with HCM had a higher resting heart rate (71 vs. 62 beats/min; p < 0.001), were more likely to have QRS duration >140 ms (12.5% vs. 0.09%; p < 0.001), a QTc interval >480 ms (14.6% vs. 0.4%; p < 0.001), left or right axis deviation, right bundle branch block, and right or left atrial abnormality.
Medicare reimbursement cuts have been associated with declining gonadotropin-releasing hormone (GnRH) agonist overuse in localized prostate cancer. Medical school affiliation and foreign training ...have been associated with persistent overuse. However, physician-level prescribing changes and the practice type of persistent overusers have not been examined. We sought to describe physician-level changes in GnRH agonist overuse and test the association of time in practice and solo practice type with GnRH agonist overuse.
We matched American Medical Association physician data for 2138 urologists to Surveillance, Epidemiology and End Result-Medicare data for 12,943 men diagnosed with early-stage and lower-grade adenocarcinoma of the prostate between 2000 and 2007. We conducted a population-based, retrospective study using multilevel modeling to control for patient and provider characteristics.
Three distinct patterns of GnRH agonist overuse were observed. Urologists' time in practice was not associated with GnRH agonist overuse (odds ratio (OR) 0.89; 95% confidence interval (CI): 0.75-1.05). However, solo practice type (OR 1.65; 95% CI: 1.34-2.02), medical school affiliation (OR 0.65; 95% CI: 0.55-0.77) and patient race were. Compared with non-Hispanic whites, non-Hispanic blacks (OR 1.76; 95% CI: 1.37-2.27), Hispanics (OR 1.41; 95% CI: 1.12-1.79) and men of 'other' race (OR 1.44; 95% CI: 1.04-1.99) had greater odds of receiving unnecessary GnRH agonists.
GnRH agonist overuse remains high among some urologists who may be professionally isolated and difficult to reach. These urologists treat more vulnerable populations, which may contribute to health disparities in prostate cancer treatment quality. Nonetheless, these findings provide guidance to develop interventions to address overuse in prostate cancer.
Supernova (SN) 2009ig was discovered 17 hr after explosion by the Lick Observatory Supernova Search, promptly classified as a normal Type Ia SN (SN Ia), peaked at V = 13.5 mag, and was equatorial, ...making it one of the foremost SNe for intensive study in the last decade. Here, we present ultraviolet (UV) and optical observations of SN 2009ig, starting about 1 day after explosion until around maximum brightness. Our data include excellent UV and optical light curves, 25 premaximum optical spectra, and 8 UV spectra, including the earliest UV spectrum ever obtained of an SN Ia. SN 2009ig is a relatively normal SN Ia, but does display high-velocity ejecta--the ejecta velocity measured in our earliest spectra (v --2,000 km s--1 for Si II Delta *l6355) is the highest yet measured in an SN Ia. The spectral evolution is very dramatic at times earlier than 12 days before maximum brightness, but slows after that time. The early-time data provide a precise measurement of 17.13 ? 0.07 days for the SN rise time. The optical color curves and early-time spectra are significantly different from template light curves and spectra used for light-curve fitting and K-corrections, indicating that the template light curves and spectra do not properly represent all SNe Ia at very early times. In the age of wide-angle sky surveys, SNe like SN 2009ig that are nearby, bright, well positioned, and promptly discovered will still be rare. As shown with SN 2009ig, detailed studies of single events can provide significantly more information for testing systematic uncertainties related to SN Ia distance estimates and constraining progenitor and explosion models than large samples of more distant SNe.
Prion protein consists of an ensemble of glycosylated variants or glycoforms. The enzymes that direct oligosaccharide processing, and hence control the glycan profile for any given glycoprotein, are ...often exquisitely sensitive to other events taking place within the cell in which the glycoprotein is expressed. Alterations in the populations of sugars attached to proteins can reflect changes caused, for example, by developmental processes or by disease. Here we report that normal (PrPC) and pathogenic (PrPSc) prion proteins (PrP) from Syrian hamsters contain the same set of at least 52 bi-, tri-, and tetraantennary N-linked oligosaccharides, although the relative proportions of individual glycans differ. This conservation of structure suggests that the conversion of PrPC into PrPSc is not confined to a subset of PrPs that contain specific sugars. Compared with PrPC, PrPSc contains decreased levels of glycans with bisecting GlcNAc residues and increased levels of tri- and tetraantennary sugars. This change is consistent with a decrease in the activity of N-acetylglucosaminyltransferase III (GnTIII) toward PrPC in cells where PrPSc is formed and argues that, in at least some cells forming PrPSc the glycosylation machinery has been perturbed. The reduction in GnTIII activity is intriguing both with respect to the pathogenesis of the prion disease and the replication pathway for prions.
15-Lipoxygenase (15-LOX)-2 is expressed in benign prostate secretory cells and benign prostate produces 15S-hydroxyeicosatetraenoic acid (15S-HETE) from exogenous arachidonic acid (AA). In contrast, ...15S-LOX-2 and 15S-HETE formation are reduced in prostate carcinoma (Pca). The mechanisms whereby reduced 15-LOX-2 may contribute to Pca development or progression are not known. We investigated the expression of peroxisome proliferator-activated receptor (PPAR) gamma in benign and malignant prostate tissues and the ability of 15S-HETE to activate PPARgamma-dependent transcription and modulate proliferation of the Pca cell line PC3. In contrast to benign prostate and similar to most Pca tissues, 15-LOX-2 mRNA was not detected in PC3 cells, and they did not produce detectable 15-HETE from 14CAA. By reverse transcription-PCR, PPARgamma mRNA was present in 18 of 18 benign and 9 of 9 tumor specimens. The PPARgamma ligand BRL 49653 and 15S-HETE caused a dose-dependent inhibition of PC3 proliferation in a 14-day soft agar colony-forming assay (IC50 of 3 and 30 microM, respectively). 15S-HETE (10 microM) caused greater inhibition than 10 microM 15R-HETE. At 3 days, BRL 49653 and 15S-HETE caused a slight increase in cells in G0-G1 and a corresponding decrease in cells in S phase. In PC3 cells transiently transfected with a luciferase reporter linked to a PPAR response element, 1 microM BRL 49653 and 10 microM 15S-HETE caused approximately threefold and greater than twofold induction of PPAR-dependent transcription, respectively. By quantitative real-time reverse transcription-PCR and Northern analysis, 3-day treatment with BRL 49653 and 15S-HETE caused a reduction of PPARgamma expression but a marked up-regulation of the PPAR response element containing adipocyte type fatty acid binding protein. These results support the hypothesis that 15-LOX-2-derived 15S-HETE may constitute an endogenous ligand for PPARgamma in the prostate and that loss of this pathway by reduced expression of 15-LOX-2 may contribute to increased proliferation and reduced differentiation in prostate carcinoma.
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