Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for ...causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch’s Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.
This study investigated heat shock-induced resistance (HSIR) against cucumber powdery mildew and confirmed the initial mechanism of HSIR prior to systemic acquired resistance. Heat shock treatment ...reduced the disease index of powdery mildew when the inoculation period was 24 h or less. HSIR increased the gene expression of Hsp70 12 h after the treatment, while the gene expression levels of POX and LOX6 increased 24 h after the treatment. Heat shock element (HSE) motifs were found in the promoter area of these target genes. The gene expression levels of HsfA2, HsfB1, and HsfB4 increased 6 h after heat shock. Therefore, upon heat stress, some heat shock transcription factors were activated to bind to HSEs and upregulate the transcription of Hsp70 and defense-related genes.
•Heat shock treatment induced cucumber resistance against powdery mildew by upregulating the expression of Hsfs.•HsfA2, HsfB1, and HsfB4 acted as inducible Hsf genes in cucumber in heat stress response at very early stages.•Hsfs induced the gene expression of Hsp70, LOX6, and POX, increasing the resistance of cucumbers against powdery mildew.•Heat shock element (HSE) binding motifs (“GAAnnTTC”) were discovered in promoter area of cucumber defense-related genes.•HSE motifs possibly led the direct binding of Hsfs to HSEs before the transcription of defense-related genes in cucumber.
Ultraviolet-B (UV-B) light is known to induce plant resistance in suppressing disease infection. Its ability to induce plant resistance has been widely used as an inducer of plant resistance. This ...study aims to determine the ability of UV-B light in suppressing shallot twisted disease, potency of plant resistance induction through accumulation of peroxidase enzymes, salicylic acid, chlorophyl content and to observe shallot growth after treatment. This study applied UV-B light with a duration of 65 mW/m2 for 4 hours each day for 3, 5 and 7 days, for inoculated and non-inoculated plants. Fungicide (Benomyl) was used as a comparison for inoculated plants. The results showed that UV-B irradiation of 4 hours/day for 7 days was able to reduce the incidence of shallot twisted disease, but the incubation period among treatments was not significantly different. UV-B irradiation of 4 hours/day, for 7 days was able to increase salicylic acid content, and maintain the same chlorophyll content as the negative control, however the peroxidase enzyme content was not significantly different. Treatment of UV-B 4 hours/day for 7 days irradiation did not inhibit the growth and production of shallot plants.
This study aimed to comprehend the diversity of the fungal community on Chokanan mango, a premium mango variety from Thailand which is widely cultivated in Indonesia, associated with stem end rot and ...anthracnose disease using high-throughput amplicon targeted metagenomics analysis by next-generation sequencing (NGS). Samples used in this study were freshly harvested healthy fruits at the age of 15-weeks (H15.ITS), healthy fruits after 2 weeks incubation (H17.ITS), 17-week old fruits (S17.ITS) with stem end rot symptoms, and 17-week old fruits (A17.ITS) with anthracnose symptoms. Results showed that the Basidiomycota phylum was dominant in the healthy fruits, while the Ascomycota phylum was found dominantly in sick fruits. Based on OTUs alignment of sequenced data, some species found to be dominantly associated with stem end rot disease in this study were Lasiodiplodia theobromae, Neofusicoccum cordaticola and N. mangiferae. Dominant species which were associated with mango anthracnose disease were Colletotrichum gloeosporioides, Botryosphaeria corticis, Volutella sp., and Pseudofusicoccum violacearum. These fungal genera were not found to be dominant in healthy fruits at the same age indicating that specific genera contributed to developing postharvest diseases on mango differently. The findings confirmed that the fungal community associated with stem end rot and anthracnose disease on mango was unique, and specific species contributed in particular disease development. Since mango is an important global commodity, these research findings will contribute significantly to global biosecurity.
Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. ...The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.
Phytophthora and Pythium are a group of Oomycetes that are widely associated with diseases in potato plants. Therefore, this study was conducted to identify the Oomycetes associated with the ...rhizosphere of infected potato plants showing leaf blight. Four isolates were collected from four regions in Central Java (UGM_St_TM, UGM_St_BK, UGM_St_BNJ, and UGM_St_KJ, the isolate from Temanggung, Bakal, Banjarnegara, Kejajar respectively), and UGM_St_NG isolate as culture collection from Laboratory of Plant Pathology. Molecular identification of all isolates was carried out using the internal transcribed spacer (ITS1/ITS4), nuclear large-ribosomal subunit (LSU), and cytochrome C oxidase subunit 1 (COX1) gene markers. Based on the results, the isolates UGM_St_TM, UGM_St_BK, and UGM_St_BNJ were identified as Phythium ultimum while UGM_St_KJ and UGM_St_NG isolate were identified as Phytopythium vexans.
Salah satu kendala utama dalam budi daya tanaman stroberi di Indonesia adalah banyaknya penyakit tanaman. Penelitian ini merupakan lanjutan dari laporan tentang penyakit penting pada buah stroberi ...(Fragaria ananassa) di Kabupaten Purbalingga, Jawa Tengah untuk mengidentifikasi penyakit oleh cendawan pada bagian tanaman selain buah. Sampel berupa daun atau tanaman bergejala, diidentifikasi secara morfologi atau molekuler apabila identifikasi morfologi tidak memungkinkan. Konfirmasi gejala penyakit yang disebabkan oleh cendawan dilakukan dengan mengikuti postulat Koch, yaitu menginokulasikan cendawan patogen hasil isolasi pada tanaman stroberi sehat. Hasil penelitian menunjukkan bahwa penyakit penting yang disebabkan oleh cendawan pada tanaman stroberi adalah penyakit hawar daun yang disebabkan oleh Phomopsis sp. dan Pestalotia sp.; penyakit gosong daun yang disebabkan oleh Curvularia sp. dan Diplocarpon sp.; penyakit bercak daun yang disebabkan oleh Cercospora sp., Phoma sp., dan Gnomonia sp.; serta penyakit layu yang disebabkan oleh Verticillium sp. dan Fusarium sp. Selain laporan tentang penyakit penting tanaman stroberi, ini juga merupakan laporan pertama tentang penyakit bercak Gnomonia pada tanaman stroberi di Indonesia.
Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. ...The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.
Pisang abaka (Musa textilis) merupakan penghasil serat alam berkualitas tinggi dengan permasalahan utama penyakit layu fusarium oleh Fusarium oxysporum f. sp. cubense TR4. Penelitian ini bertujuan ...memperoleh bakteri pelarut silika (BPS), menentukan pengaruh aplikasi BPS dan pupuk silika untuk menghambat serangan fusarium serta memacu pertumbuhan tanaman pisang. Sebanyak enam isolat BPS diperoleh dari tanah rizosfer pisang abaka sehat, tiga di antaranya memiliki aktivitas antagonis terhadap F. oxysporum f. sp. cubense TR4. Empat perlakuan yang dicobakan menunjukkan keparahan penyakit layu fusarium yang tidak berbeda. Demikian juga keempat perlakuan tidak menunjukkan perbedaan pada peubah pertumbuhan yang diamati. Bakteri pelarut silika dan pupuk silika belum mampu mengendalikan F. oxysporum f. sp. cubense TR4. Oleh karena itu, pisang abaka terbukti rentan terhadap F. oxysporum f. sp. cubense TR4.
Fruit set is one of the main problems that arise in tomato plants under heat-stress conditions, which disrupt pollen development, resulting in decreased pollen fertility. Parthenocarpic tomatoes can ...be used to increase plant productivity during failure of the fertilisation process under heat-stress conditions. The aim of this study were to identify the plant adaptability and fruiting capability of ?
and
tomato mutants under heat-stress conditions. The
and
and wild-type Micro-Tom (WT-MT) plants were cultivated under two temperature conditions: normal and heat-stress conditions during plant growth. The results showed that under the heat-stress condition,
and
showed delayed flowering time, increased number of flowers, and increased fruit set and produced normal-sized fruit. However, WT-MT cannot produce fruits under heat stress. The mutants can grow under heat-stress conditions, as indicated by the lower electrolyte leakage and H
O
concentration and higher antioxidant activities compared with WT-MT under heat-stress conditions. These results suggest that
and
can be valuable genetic resources for the development of tomatoes in high-temperature environmental conditions.
