A new general strategy based on the use of multiparameter fluorescence detection (MFD) to register and quantitatively analyse fluorescence images is introduced. Multiparameter fluorescence imaging ...(MFDi) uses pulsed excitation, time-correlated single-photon counting and a special pixel clock to simultaneously monitor the changes in the eight-dimensional fluorescence information (fundamental anisotropy, fluorescence lifetime, fluorescence intensity, time, excitation spectrum, fluorescence spectrum, fluorescence quantum yield, distance between fluorophores) in real time. The three spatial coordinates are also stored. The most statistically efficient techniques known from single-molecule spectroscopy are used to estimate fluorescence parameters of interest for all pixels, not just for the regions of interest. Their statistical significance is judged from a stack of two-dimensional histograms. In this way, specific pixels can be selected for subsequent pixel-based subensemble analysis in order to improve the statistical accuracy of the parameters estimated. MFDi avoids the need for sequential measurements, because the registered data allow one to perform many analysis techniques, such as fluorescence-intensity distribution analysis (FIDA) and fluorescence correlation spectroscopy (FCS), in an off-line mode. The limitations of FCS for counting molecules and monitoring dynamics are discussed. To demonstrate the ability of our technique, we analysed two systems: (i) interactions of the fluorescent dye Rhodamine 110 inside and outside of a glutathione sepharose bead, and (ii) microtubule dynamics in live yeast cells of Schizosaccharomyces pombe using a fusion protein of Green Fluorescent Protein (GFP) with Minichromosome Altered Loss Protein 3 (Mal3), which is involved in the dynamic cycle of polymerising and depolymerising microtubules.
•New 2,6-dimethylpyrazine - picric acid cocrystal was synthezed and investigated.•DFT calculation for solids state model was performed.•Low temperature phase transition was observed.•Full vibrational ...analysis was performed.•Activation energy for reorientation of CCH3bending vibration was obtained.
In the present paper the structural and dynamical properties of crystalline 2,6–dimethylpyrazine - picric acid cocrystal are described. The title cocrystal crystallizes in centrosymmetric monoclinic P21/c space group. The X-ray diffraction and NMR spectroscopy show the presence of protonated form of 2,6-dimethypyrazine and deprotonated form of picric acid molecules. The Hirshfeld surface analysis for determine of intermolecular contacts was performed. Low temperature phase transition was observed and described by temperature dependent investigations: X-ray diffraction, different scanning calorimetry (DSC) and IR spectroscopy. The vibrational properties of cocrystal were investigated by use of IR and Raman spectroscopies, as well as density functional theory (DFT) with periodic boundary conditions. Activation energy for reorientational motion of CH3 group was determined from the band shape analysis of CCH3 bending vibration.
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Results of structural, magnetic and specific heat investigations of the potassium erbium double tungstate, KEr(WO4)2, are presented. Potassium erbium double-tungstate KEr(WO4)2 single crystals have ...been grown by the top-seeded solution growth method (TSSG) and modified Czochralski techniques. It crystallizes in the monoclinic crystal structure (C 2/c space group). The unit cell contains four formula units and is described by parameters a = 10.615(2) A, b = 10.316(2) A, c = 7.534(2) A, beta = 130.73(3) deg . From the x-ray diffraction measurements the fractional atomic coordinates, displacement parameters and interatomic distances have been determined.
The PHOBOS detector at RHIC Back, B.B; Baker, M.D; Barton, D.S ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
03/2003, Letnik:
499, Številka:
2
Journal Article
Recenzirano
This manuscript contains a detailed description of the PHOBOS experiment as it is configured for the Year 2001 running period. It is capable of detecting charged particles over the full solid angle ...using a multiplicity detector and measuring identified charged particles near mid-rapidity in two spectrometer arms with opposite magnetic fields. Both of these components utilize silicon pad detectors for charged particle detection. The minimization of material between the collision vertex and the first layers of silicon detectors allows for the detection of charged particles with very low transverse momenta, which is a unique feature of the PHOBOS experiment. Additional detectors include a time-of-flight wall which extends the particle identification range for one spectrometer arm, as well as sets of scintillator paddle and Cherenkov detector arrays for event triggering and centrality selection.
In this article the results concerned influence of processing conditions of the wire electrical discharge machining and vibro‐abrasive machining on the surface layer and morphology of samples made of ...hard machinable materials used in aircraft industry like: Titanium 5553 β, Inconel 617, Hastelloy X and Magnesium AZ31 have been presented. For this purpose the cubic and cylindrical samples made of hard machinable alloys have been prepared using optimal electric parameters of wire‐cut electrical discharge machining and finally they have been polished using circular vibratory finishing technology and different ceramic shaped stones.
Translation
Im Artikel werden Untersuchungsergebnisse von schwer zu bearbeitenden Metalllegierungen aus der Flugzeugindustrie nach dem Einsatz von elektroerosivem Drahtschneiden und der Vibrationsschleiftechnik in Bezug auf Rauheit und Oberflächenstruktur dargestellt. Zu diesen gehören: Titan 5553 ß‐Legierung, Inconel 617, Hastelloy X und Magnesium AZ31‐Legierung. Zu diesem Zweck wurden Proben in Würfel‐ und Zylinderform mit elektroerosivem Drahtschneiden ausgeschnitten und mittels der Vibrationsschleiftechnik unter Anwendung von keramischen Schleifmitteln fertigbearbeitet.
1-phenyl-2H,6H-imidazo1,5-cquinazoline-3,5-dione reacts with ethyl bromoacetate under mild conditions to give 2-(ethoxycarbonylmethyl)-1-phenyl-6H-imidazo1,5-cquinazoline-3,5-dione (MEPIQ) and next ...2,6-bis(ethoxycarbonylmethyl)-1-phenylimidazo1,5-cquinazoline-3,5-dione (BEPIQ). The products were isolated at high yield and identified on the basis of IR,
1
H- and
13
C-NMR, UV spectroscopy, and X-ray crystallography. Diester (BEPIQ) can be presented by 16 possible pair of enantiomers. Only one pair of them is the most stable and crystallizes which is shown crystallographic research. Based on quantum-mechanical modeling, with the use of DFT method, which conformers of mono- and diester and why they were formed was explained. It was calculated that 99.93% of the monoester (MEPIQ) is formed at position No. 2 and one pair of the monoester conformers, from six possible, has the largest share (51.63%). These results afforded to limit the number of diester conformers to eight. Unfortunately, the quantum-mechanical calculations performed that their shares are similar. Further quantum-mechanical modeling showed that conformers are able to undergo mutual transformations. As a result only one pair of diester conformers forms crystals. These conformers have substituents in trans position and these substituents are located parallel to imidazoquinazoline ring. This allows for the denser packing of the molecules in the unit cell.
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