Recent results from the MAVEN Langmuir Probe and Waves instrument suggest higher than predicted electron temperatures (Te) in Mars' dayside ionosphere above ~180 km in altitude. Correspondingly, ...measurements from Neutral Gas and Ion Mass Spectrometer indicate significant abundances of O2+ up to ~500 km in altitude, suggesting that O2+ may be a principal ion loss mechanism of oxygen. In this article, we investigate the effects of the higher Te (which results from electron heating) and ion heating on ion outflow and loss. Numerical solutions show that plasma processes including ion heating and higher Te may greatly increase O2+ loss at Mars. In particular, enhanced Te in Mars' ionosphere just above the exobase creates a substantial ambipolar electric field with a potential (eΦ) of several kBTe, which draws ions out of the region allowing for enhanced escape. With active solar wind, electron, and ion heating, direct O2+ loss could match or exceed loss via dissociative recombination of O2+. These results suggest that direct loss of O2+ may have played a significant role in the loss of oxygen at Mars over time.
Key Points
O2+ outflow at Mars is greatly enhanced by ambipolar electric field
Enhanced electron temperatures result in a strong ambipolar electric field
In the past, direct loss of O2+ could have exceeded that of associative recombination
•Analyzed the radial and seasonal dependence of Saturn’s thermal plasma.•Showed the plasma between the edge of the main rings and Mimas is dynamic.•Found the region outside the main rings is a plasma ...sink.•Plasma quenching due to the presence of small grains.
A goal of Cassini’s extended mission is to examine the seasonal variations of Saturn’s magnetosphere, moons, and rings. Recently we showed that the thermal plasma between the main rings and Enceladus exhibited a time dependence that we attributed to a seasonally variable source of oxygen from the main rings (Elrod, M.K., Tseng, W.-L., Wilson, R.J., Johnson, R.E. 2012. J. Geophys. Res. 117, A03207. http://dx.doi.org/10.1029/2011JA017332). Such a temporal variation was subsequently seen in the energetic ion composition (Christon, S.P., Hamilton, D.C., DiFabio, R.D., Mitchel, D.G., Krimigis, S.M., Jontof-Hutter, D.S. 2013. J. Geophys. Res. 28. http://dx.doi.org/10.1002/jgra.50383; Christon, S.P., Hamilton, D.C., Mitchell, D.G., DiFabio, R.D., Krimigis, S.M. 2014. J. Geophys. Res., submitted for publication). Here we incorporate the most recent measurements by the Cassini Plasma Spectrometer (CAPS) into our earlier analysis (Elrod, M.K., Tseng, W.-L., Wilson, R.J., Johnson, R.E. 2012. J. Geophys. Res. 117, A03207. http://dx.doi.org/10.1029/2011JA017332) and our modeling (Tseng, W.-L., Johnson, R.E., Elrod, M.K. 2013a. Planet. Space Sci. 77, 126–135) of the thermal plasma in the region between the main rings and the orbit of Enceladus. Data taken in 2012, well past equinox for which the northern side of the main rings were illuminated, appear consistent with a seasonal variation. Although the thermal plasma in this region comes from two sources that have very different radial and temporal trends, the extended ring atmosphere and the Enceladus torus, the heavy ion density is found to exhibit a steep radial dependence that is similar for the years examined. Using our chemical model, we show that this dependence requires either a radial dependence for Enceladus torus that differs significantly from recent models or, as we suggest here, enhanced heavy ion quenching/neutralization with decreasing distance from the edge of the main rings. We examine the possible physical processes and suggest that the presence of small grains and the precipitation of the inward diffusing high-energy background radiation onto the edge of the main rings play important roles.
Aims
In an attempt to devise decontamination methods that are both effective and minimally detrimental to the environment, we evaluated germination induction as an enhancement to strategies for ...Bacillus anthracis spore decontamination. To determine an optimal method for the recovery of germinating spores from different matrices, it was critical to ensure that the sampling procedures did not negatively impact the viability of the germinating spores possibly confounding the results and downstream analyses of field trial data.
Methods and Results
Therefore, the two main objectives of this study were the following: (i) development of an effective processing protocol capable of recovering the maximum number of viable germinating or germinated spores from different surface materials; and (ii) using a model system of spore contamination, employ this protocol to evaluate the potential applicability of germination induction to wide‐area decontamination of B. anthracis spores. We examined parameters affecting the sampling efficiencies of B. anthracis and the surrogate species Bacillus thuringiensis on nonporous and porous materials.
Conclusions
The most efficient extraction from all matrices was observed using PBS with 0·01% Tween 80 extraction buffer. The addition of a sonication and/or extended vortex treatment did not yield significant increases in spore or germinated spore recovery.
Significance and Impact of the Study
Our data demonstrate that previous germination‐induction experiments performed in suspension can be reproduced when Bacillus spores are deposited onto reference surfaces materials. Our proof of concept experiment illustrated that a germination pretreatment step significantly improves conventional secondary decontamination strategies and remediation plans.
We investigate the complex interaction between Saturn's magnetosphere and Titan's upper ionosphere using ion data acquired by the Cassini Plasma Spectrometer (CAPS) during the T40 encounter. Bounds ...on ion‐group abundances at altitudes between ~2733 and ~12,541 km are determined by fitting mass spectra with model functions derived from instrument calibration data. The spectra are dominated by H+, H2+, H3+, and two hydrocarbon groups with mass ranges 12–19 and 24–32 amu, respectively. Notably, this constitutes the first reported observation of H3+ in Titan's exosphere. These measurements are discussed in the context of data from the CAPS electron spectrometer (ELS) and the Ion and Neutral Mass Spectrometer (INMS), which fortuitously sampled the ionospheric outflow during the T40 encounter at altitudes between ~2225 and ~3034 km. The CAPS data reveal a composition that is constitutively similar to that sampled by INMS, with hydrocarbon ions first observed as far as ~11,000 km from Titan and increasing in density by more than an order of magnitude along Cassini's inbound trajectory. In addition, we juxtapose the CAPS ion data with numerical results from three different interaction models and show that it is consistent with the location of the field‐draping boundary described by Ulusen et al. (2012) and the Saturnward ion tail predicted by Sillanpää et al. (2006).
Key Points
Ion densities extracted from CAPS IMS data acquired during T40 Titan encounter
IMS abundances consistent with concurrent CAPS ELS and INMS density measurements
Data imply presence of Saturnward ion tail in agreement with MHD simulations
Sea breezes often have significant impacts on nearshore physical and biological processes. We document the effects of a diurnal sea breeze on the nearshore thermal structure and circulation of ...northern Monterey Bay, California, using an array of moorings during the summer upwelling season in 2006. Moorings were equipped with thermistors and Acoustic Doppler Current Profilers (ADCPs) to measure temperature and currents along the inner shelf in the bay. Temperature and current data were characteristic of traditional regional scale upwelling conditions along the central California coast during the study period. However, large diurnal fluctuations in temperature (up to 5
°C) were observed at all moorings inshore of the 60-m isobath. Examination of tidal, current, temperature, and wind records revealed that the observed temperature fluctuations were the result of local diurnal upwelling, and not a result of nearshore mixing events. Westerly diurnal sea breezes led to offshore Ekman transport of surface waters. Resulting currents in the upper mixed layer were up to 0.10
m
s
−1 directed offshore during the afternoon upwelling period. Surface water temperatures rapidly decreased in response to offshore advection of surface waters and upwelling of cold, subsurface water, despite occurring in the mid-afternoon during the period of highest solar heat flux. Surface waters then warmed again during the night and early morning as winds relaxed and the upwelling shadow moved back to shore due to an unbalanced onshore pressure gradient. Examination of season-long, moored time series showed that local diurnal upwelling is a common, persistent feature in this location. Local diurnal upwelling may supply nutrients to nearshore kelp beds, and transport larvae to nearshore habitats.
In the absence of direct atmospheric temperature measurements, density data along spacecraft trajectories are often used to extract temperature profiles, a process that can fail in the presence of ...wave activity (Leclercq et al., 2020). Because spacecraft data are often not sufficient to uniquely characterize wave-like perturbations, we examine the difference between predicted and extracted temperature profiles based on linearized solutions to the wave equations and briefly discuss the relevance to wave activity in the upper atmospheres of Mars and Titan. We find that the extracted temperatures are significantly overestimated for the full range of acoustic gravity wave parameters but are somewhat underestimated for a range of internal gravity wave parameters, and the density amplitudes can serve as a proxy for the extracted temperature amplitudes when estimating the heating rates.
•The local temperatures in the upper atmospheres of planets extracted from density data using the hydrostatic approximation can be incorrect in the presence of wave activity•Using a 2D linear model we examine when that method fails in the presence of significant wave activity in the upper atmospheres of Mars and Titan
To determine clinical and immunologic responses to a multipeptide melanoma vaccine regimen, a randomized phase II trial was performed.
Twenty-six patients with advanced melanoma were randomly ...assigned to vaccination with a mixture of four gp100 and tyrosinase peptides restricted by HLA-A1, HLA-A2, and HLA-A3, plus a tetanus helper peptide, either in an emulsion with granulocyte-macrophage colony-stimulating factor (GM-CSF) and Montanide ISA-51 adjuvant (Seppic Inc, Fairfield, NJ), or pulsed on monocyte-derived dendritic cells (DCs). Systemic low-dose interleukin-2 (Chiron, Emeryville, CA) was given to both groups. T-lymphocyte responses were assessed, by interferon gamma ELIspot assay (Chiron, Emeryville, CA), in peripheral-blood lymphocytes (PBLs) and in a lymph node draining a vaccine site (sentinel immunized node SIN).
In patients vaccinated with GM-CSF in adjuvant, T-cell responses to melanoma peptides were observed in 42% of PBLs and 80% of SINs, but in patients vaccinated with DCs, they were observed in only 11% and 13%, respectively. The overall immune response was greater in the GM-CSF arm (P <.02). Vitiligo developed in two of 13 patients in the GM-CSF arm but in no patients in the DC arm. Helper T-cell responses to the tetanus peptide were detected in PBLs after vaccination and correlated with T-cell reactivity to the melanoma peptides. Objective clinical responses were observed in two patients in the GM-CSF arm and one patient in the DC arm. Stable disease was observed in two patients in the GM-CSF arm and one patient in the DC arm.
The high frequency of cytotoxic T-lymphocyte responses and the occurrence of clinical tumor regressions support continued investigation of multipeptide vaccines administered with GM-CSF in adjuvant.
A phase II trial was performed to test whether systemic low-dose interleukin-2 (IL-2) augments T-cell immune responses to a multipeptide melanoma vaccine. Forty patients with resected stage IIB-IV ...melanoma were randomly assigned to vaccination with four gp100- and tyrosinase-derived peptides restricted by human leukocyte antigen (HLA) -A1, HLA-A2, and HLA-A3, and a tetanus helper peptide plus IL-2 administered daily either beginning day 7 (group 1), or beginning day 28 (group 2).
T-cell responses were assessed by an interferon gamma ELIspot assay in peripheral blood lymphocytes (PBL) and in a lymph node draining a vaccination site (sentinel immunized node SIN). Patients were followed for disease-free and overall survival.
T-cell responses to the melanoma peptides were observed in 37% of PBL and 38% of SINs in group 1, and in 53% of PBL and 83% of SINs in group 2. The magnitude of T-cell response was higher in group 2. The tyrosinase peptides DAEKSDICTDEY and YMDGTMSQV were more immunogenic than the gp100 peptides YLEPGPVTA and ALLAVGATK. T-cell responses were detected in the SINs more frequently, and with higher magnitude, than responses in the PBL. Disease-free survival estimates at 2 years were 39% (95% CI, 18% to 61%) for group 1, and 50% (95% CI, 28% to 72%) for group 2 (P = .32).
The results of this study support the safety and immunogenicity of a vaccine composed of four peptides derived from gp100 and tyrosinase. The low-dose IL-2 regimen used for group 1 paradoxically diminishes the magnitude and frequency of cytotoxic T lymphocyte responses to these peptides.
We describe characterization of backscatter from tumor tissue targeted with a nanoparticle-based ultrasound contrast agent in vivo using analogs of thermodynamic quantities. We apply these waveform ...characteristics to detection of tumor neovasculature in tumors implanted in athymic nude mice, which were imaged using a research ultrasound scanner over a 2-hour period after injection of alpha nu beta 3 -targeted perfluorocarbon nanoparticles. Images were constructed from backscattered ultrasound using two different approaches: fundamental B-mode imaging and a signal receiver based on a thermodynamic analog (H c ). The study shows that the thermodynamic analog is capable of detecting differences in backscattered signals that are not apparent with the B-mode approach
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