Photothermal therapy using nanoparticles is a promising new approach for the treatment of cancer. The principle is to utilise plasmonic nanoparticle light interaction for efficient heat conversion. ...However, there are many hurdles to overcome before it can be accepted in clinical practice. One issue is a current poor characterization of the thermal dose that is distributed over the tumour region and the surrounding normal tissue. Here, we use Monte Carlo simulations of photon radiative transfer through tissue and subsequent heat diffusion calculations, to model the spatial thermal dose in a skin cancer model. We validate our heat rise simulations against experimental data from the literature and estimate the concentration of nanorods in the tumor that are associated with the heat rise. We use the cumulative equivalent minutes at 43 °C (CEM43) metric to analyse the percentage cell kill across the tumour and the surrounding normal tissue. Overall, we show that computer simulations of photothermal therapy are an invaluable tool to fully characterize thermal dose within tumour and normal tissue.
Artificial light at night (ALAN), from streetlights and other sources, has a wide variety of impacts on the natural environment. A significant challenge remains, however, to predict at intermediate ...spatial extents (e.g. across a city) the ALAN that organisms experience under different lighting regimes. Here we use Monte Carlo radiative Transfer to model the three-dimensional lighting environment at, and just above, ground level, on the spatial scales at which animals and humans experience it. We show how this technique can be used to model a suite of both real and hypothetical lighting environments, mimicking the transition of public infrastructure between different lighting technologies. We then demonstrate how the behaviour of animals experiencing these simulated lighting environments can be emulated to probe the availability of darkness, and dark corridors, within them. Our simulations show that no single lighting technology provides an unmitigated alleviation of negative impacts within urban environments, and that holistic treatments of entire lighting environments should be employed when understanding how animals use and traverse them.
In this study, Monte Carlo simulations were created to investigate the distribution of Raman signals in tissue phantoms and to validate the arctk code that was used. The aim was to show our code is ...capable of replicating experimental results in order to use it to advise similar future studies and to predict the outcomes. The experiment performed to benchmark our code used large volume liquid tissue phantoms to simulate the scattering properties of human tissue. The scattering agent used was Intralipid (IL), of various concentrations, filling a small quartz tank. A thin sample of PTFE was made to act as a distinct layer in the tank; this was our Raman signal source. We studied experimentally, and then reproduced
via
simulations, the variation in Raman signal strength in a transmission geometry as a function of the optical properties of the scattering agent and the location of the Raman material in the volume. We have also found that a direct linear extrapolation of scattering coefficients between concentrations of Intralipid is an incorrect assumption at lower concentrations when determining the optical properties. By combining experimental and simulation results, we have calculated different estimates of these scattering coefficients. The results of this study give insight into light propagation and Raman transport in scattering media and show how the location of maximum Raman signal varies as the optical properties change. The success of arctk in reproducing observed experimental signal behaviour will allow us in future to inform the development of noninvasive cancer screening applications (such as breast and prostate cancers)
in vivo
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Monte Carlo modelling of Raman spectroscopy in tissue phantoms to validate code and provide insight into the optical properties of Intralipid.
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