•A model is proposed to describe the water-heat coupling process during freezing.•Unfrozen water function for saline soil is determined and applied in modeling.•Frozen depth of the saline region is ...predicted based on the coupling analysis.•Finale ice formation and water dynamics are simulated and validated.•Temperature variation hysteresis in soil profile is proved and analysed.
Frozen depth has a great significance for the foundation engineering in cold regions, always showing a high correlation with some attendant engineering phenomena, including water aggregation, frost heave, and salt accumulation. To study the heat-water dynamics and frozen depth characteristics during the freezing process, soils in western Jilin Province of China, a typical seasonal frozen region, were selected for investigation. A coupled heat and water model was proposed to describe the water-heat coupling process during freezing, with full consideration of the unfrozen water variation, the ice layer formation, and the interaction among different elements. Then, the dynamics of the heat-water and frozen depth were simulated based on the boundary conditions of temperature variation with reference to the meteorological data. The in- situ monitoring data from the whole winter were used to analyse the model performance. The results show that water content and temperature data match the test data, and the Root Mean Square Error (RMSE) values of the temperatures (within 2 °C) at different depths were acceptable, indicating that the water-heat dynamics can predict the maximum frozen depth well. In addition, the temperature of the soil profile varies rapidly in the first 60 days of winter, and the frozen depth continues to increase even though the temperature starts to rise after freezing for 80 days. The moisture transfers upwards with the effect of heat flow, and the formation of ice occurred mainly at a depth of 1.5 m. Heat conduction plays an important role in modelling, predominantly leading to the hysteresis in the frozen depth variation during freezing. This new method can provide a reference for water-heat movement and the prediction of the frozen depth during freezing in the saline soil regions.
Motivated by the positive psychology movement in the English as Foreign Language (EFL), existing studies have demonstrated that subject-related enjoyment has a positive correlation with academic ...achievement. However, quite a few studieshave examined why academic enjoyment can predict positive academic achievement. This study aimed to investigate whether behavioral engagement, self-concept, and organizational strategy mediated relations between academic enjoyment and achievement in EFL setting. 528 Chinese secondary school students (Male: N = 280; Female: N = 248) participated in the survey and completed the questionnaires on EFL-related enjoyment, behavioral engagement, self-concept, organizational strategy, and academic performance. Structural equation model (SEM) analyses showed that students' academic enjoyment positively predicted their English achievement. Academic engagement, self-concept, and organizational strategy had parallel multiple mediating effects between academic enjoyment and English achievement. Multi-group SEM analysis demonstrated that the model had invariance across genders, indicating that the model is applicable to both male and female students. Limitations and implications are discussed.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
The control-value theory (CVT) of achievement emotions posits that achievement emotions are significantly associated with the key indicators of academic outcomes, including academic motivation, ...engagement, and performance. Existing studies have tested the theoretical hypothesis of the CVT in a variety of cultures, disciplines, and samples. However, evidence is limited for whether there are gender and grade differences in achievement emotions, especially in the context of English as a Foreign Language (EFL). 1,460 Chinese secondary school students (male N = 671; female N = 789; seventh-graders N = 731; eighth-graders N = 729) took part in the study. Confirmatory factor analyses and multi-group analyses were conducted to explore the possible gender and grade differences in EFL-related achievement emotions. Results indicated that there are gender or grade differences in EFL-related enjoyment, anxiety, and boredom, while hope and pride did not. Both limitations and implications are discussed.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Frost heave and thaw settlement are the main cause for damaging the engineering structures in the region of seasonally frozen soil. The soil freeze-thaw (FT) deformation is a typical complex result ...of multiple physical fields and involves the process of water transfer, heat transfer, ice-water phase transition and stress redistribution. While the theory and model of frost heave have been studied for decades, less attention has been paid to the simulation for soil deformation under FT cycles. In this study, coupled equations for water, heat and the deformation of frozen soil are derived based on previous theories and are realized in numerical software. The volumetric water content of the frozen soil in the experiment was tested by nuclear magnetic resonance (NMR) technology, and the obtained data were imported into the model. Experimental soil samples were arranged in improved model equipment, and the temperature and frost heave at different heights of the soil column varied with time. The model validity and applicability based on the hydro-thermal-mechanical coupled equations is verified by comparing the results of both the experiment and numerical simulation. In this research, from the curves of the soil temperature change, water content distribution, water flux occurred at the soil bottom surface, as well as the displacement of frost heave and thaw settlement, and the pore size distribution, the characteristics of the soil subjected to cyclic FT conditions was analyzed. The existed theoretical model cannot well model the soil FT deformation. Therefore, this study may provide references for developing more accurate theoretical models on the soil subjected to FT cycles.
•A coupled hydro-thermal-mechanical analysis was conducted.•Freeze-thaw tests and mercury injection porosimetry test were carried out.•The applicability and usefulness of the model were discussed.•The deformation behavior of soil was analyzed from the micro-mechanism.
The technique of carbonated stabilization is an innovative method to improve the engineering performance of soft soils. However, the improvement in the engineering performance of stabilized soils ...with carbonized steel slag has not been studied. In addition, the effect of clay minerals on carbonation parameters and D-W cycle performance remains unknown. To this end, in this paper, three types of synthetic soils containing different clay minerals were prepared to be stabilized by carbonated steel slag. The strength performance and microstructural evolution of carbonated steel slag stabilized soils were investigated by drying-wetting (D-W) cycle tests, unconfined compression strength (UCS) tests, X-ray diffraction (XRD), thermogravimetry (TG), and scanning electron microscopy (SEM). The results showed that the compressive strength after and resistance to D-W cycles of carbonated steel slag stabilized soils were significantly improved due to carbonation treatment. The presence of clay minerals in stabilized soils led to the high values of optimum moisture content for carbonation (C-OMC). The C-OMC value of stabilized soils containing montmorillonite was higher than that of stabilized soils containing kaolinite. Montmorillonite had a higher negative effect on the D-W cycle performance of soils compared to kaolinite. This fact could be reflected by the fluctuation of UCS and mass change ratio (Rm). The main carbonation product by three types of carbonated steel slag stabilized soils was calcite, which remained stable after D-W cycles. After carbonation for 18 h, the generated calcite and carbonation degrees for three types of stabilized soils were obtained. In the microstructures of carbonated steel slag stabilized soils, the spindle-like clusters of the generated calcite appeared, which adhered to the surface of mineral particles. The aggregated degrees of the generated calcite in stabilized soils containing clay minerals tended to decrease after D-W cycles. In this work, a new type of carbonated steel slag stabilized soil was studied. The results indicated that the effect of clay minerals on the engineering characteristics of soil should be considered.
•Carbonated steel slag is used to stabilize three types of artificial soils.•Influence of clay minerals on performance and microstructure of carbonated steel slag stabilized soils is investigated.•The existence of clay minerals leads to increasing values of the optimum moisture content for carbonation.•The main carbonation product is calcite which remains to be stable after drying and wetting cycles.
The prevalence of type 2 diabetes (T2D) has been rising dramatically in many countries around the world. The main signatures of T2D are insulin resistance and dysfunction of β-cells. While there are ...several pharmaceutical therapies for T2D, no effective treatment is available for reversing the functional decline of pancreatic β-cells in T2D patients. It has been well recognized that glucagon-like peptide-1 (GLP-1), which is an incretin hormone secreted from intestinal L-cells, plays a vital role in regulating glycemic homeostasis via potentiating glucose-stimulated insulin secretion and promoting β-cell function. We found that genipin, a natural compound from Elli, can directly target intestinal L-cells, leading to the secretion of GLP-1. Incubation of the cells with genipin elicited a rapid increase in intracellular Ca
. Inhibition of PLC ablated genipin-stimulated Ca
increase and GLP-1 secretion, suggesting that genipin-induced GLP-1 release from cells is dependent on the PLC/Ca
pathway. In vivo, acute administration of genipin stimulated GLP-1 secretion in mice. Chronically, treatment with genipin via oral gavage at 50 mg/kg/day for 6 weeks reversed hyperglycemia and insulin resistance in high-fat-diet (HFD)-induced obese mice. Moreover, genipin alleviated the impaired lipid metabolism and decreased lipid accumulation in the liver of obese mice. These results suggest that naturally occurring genipin might potentially be a novel agent for the treatment of T2D and diet-induced fatty liver disease.
The thyroid hormone triiodothyronine (T
3
) activates thermogenesis by uncoupling electron transport from ATP synthesis in brown adipose tissue (BAT) mitochondria. Although T
3
can induce ...thermogenesis by sympathetic innervation, little is known about its cell autonomous effects on BAT mitochondria. We thus examined effects of T
3
on mitochondrial activity, autophagy, and metabolism in primary brown adipocytes and BAT and found that T
3
increased fatty acid oxidation and mitochondrial respiration as well as autophagic flux, mitophagy, and mitochondrial biogenesis. Interestingly, there was no significant induction of intracellular reactive oxygen species (ROS) despite high mitochondrial respiration and UCP1 induction by T
3
. However, when cells were treated with Atg5 siRNA to block autophagy, induction of mitochondrial respiration by T
3
decreased, and was accompanied by ROS accumulation, demonstrating a critical role for autophagic mitochondrial turnover. We next generated an Atg5 conditional knockout mouse model (Atg5 cKO) by injecting Ucp1 promoter-driven Cre-expressing adenovirus into Atg5
Flox/Flox
mice to examine effects of BAT-specific autophagy on thermogenesis in vivo. Hyperthyroid Atg5 cKO mice exhibited lower body temperature than hyperthyroid or euthyroid control mice. Metabolomic analysis showed that T
3
increased short and long chain acylcarnitines in BAT, consistent with increased β-oxidation. T
3
also decreased amino acid levels, and in conjunction with SIRT1 activation, decreased MTOR activity to stimulate autophagy. In summary, T
3
has direct effects on mitochondrial autophagy, activity, and turnover in BAT that are essential for thermogenesis. Stimulation of BAT activity by thyroid hormone or its analogs may represent a potential therapeutic strategy for obesity and metabolic diseases.
Abbreviations: ACACA: acetyl-Coenzyme A carboxylase alpha; AMPK: AMP-activated protein kinase; Acsl1: acyl-CoA synthetase long-chain family member 1; ATG5: autophagy related 5; ATG7: autophagy related 7; ATP: adenosine triphosphate; BAT: brown adipose tissue; cKO: conditional knockout; COX4I1: cytochrome c oxidase subunit 4I1; Cpt1b: carnitine palmitoyltransferase 1b, muscle; CQ: chloroquine; DAPI: 4ʹ,6-diamidino-2-phenylindole; DIO2: deiodinase, iodothyronine, type 2; DMEM: Dulbecco's modified Eagle's medium; EIF4EBP1: eukaryotic translation initiation factor 4E binding protein 1; Fabp4: fatty acid binding protein 4, adipocyte; FBS: fetal bovine serum; FCCP: carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone; FGF: fibroblast growth factor; FOXO1: forkhead box O1; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; Gpx1: glutathione peroxidase 1; Lipe: lipase, hormone sensitive; MAP1LC3B: microtubule-associated protein 1 light chain 3; mRNA: messenger RNA; MTORC1: mechanistic target of rapamycin kinase complex 1; NAD: nicotinamide adenine dinucleotide; Nrf1: nuclear respiratory factor 1; OCR: oxygen consumption rate; PBS: phosphate-buffered saline; PCR: polymerase chain reaction; PPARGC1A: peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Pnpla2: patatin-like phospholipase domain containing 2; Prdm16: PR domain containing 16; PRKA: protein kinase, AMP-activated; RPS6KB: ribosomal protein S6 kinase; RFP: red fluorescent protein; ROS: reactive oxygen species; SD: standard deviation; SEM: standard error of the mean; siRNA: small interfering RNA; SIRT1: sirtuin 1; Sod1: superoxide dismutase 1, soluble; Sod2: superoxide dismutase 2, mitochondrial; SQSTM1: sequestosome 1; T
3
: 3,5,3ʹ-triiodothyronine; TFEB: transcription factor EB; TOMM20: translocase of outer mitochondrial membrane 20; UCP1: uncoupling protein 1 (mitochondrial, proton carrier); ULK1: unc-51 like kinase 1; VDAC1: voltage-dependent anion channel 1; WAT: white adipose tissue
This study validated the school psychological capital (PsyCap) scale in the Chinese context and examined the predictive effect of PsyCap resources on academic engagement and achievement emotions. ...Self-report data for PsyCap resources, student engagement, enjoyment, anxiety, and boredom toward English learning were collected from 1,000 sophomores. Item-level analyses and confirmatory factor analysis were used to verify the validity of the school PsyCap scale, and structural equation modeling was applied to reveal the predictive effect of school PsyCap resources on academic engagement and achievement emotions. Results showed that the school PsyCap scale retained superior psychometric properties. Besides, PsyCap resources were demonstrated to have a positive relationship to academic engagement and enjoyment, and a negative relationship to anxiety and boredom. The effectiveness of the school PsyCap scale was verified among Chinese college students, and besides the traditional predictors, school PsyCap is also critically important for students’ academic engagement and achievement emotions. Limitations and implications are discussed.
Psychological Capital (PsyCap) has received much attention in industrial-organizational research because it is linked to a broad spectrum of adaptive work-related outcomes. However, few studies have ...been conducted on PsyCap in non-Western school contexts, especially in the ethnic minority areas of Mainland China. The present study aims to validate the school PsyCap scale, examine the latent mean differences of school PsyCap across Dai and Han groups, and verify the correlation between school PsyCap and academic engagement, achievement emotions, and academic achievement in a sample of 769 Chinese school children (Han students = 249, Dai students = 520) in the seventh and eighth grades. Results demonstrated that the Chinese version of the school PsyCap scale had excellent psychometric properties. Besides, there were no significant differences in the mean level of school PsyCap between the two cultural groups of Chinese Dai and Han. However, the multi-group second-order CFA model showed that the mean level of Dai students’ resilience was lower than that of Chinese Han. The findings also showed that school PsyCap has positive predictive effects on optimal academic outcomes (i.e., academic engagement, enjoyment, and academic achievement) but negatively affect boredom. This study enriches the understanding of school PsyCap from a cross-cultural perspective. Both theoretical and practical implications are discussed.
Recently developed approaches for highly multiplexed imaging have revealed complex patterns of cellular positioning and cell-cell interactions with important roles in both cellular- and tissue-level ...physiology. However, tools to quantitatively study cellular patterning and tissue architecture are currently lacking. Here, we develop a spatial analysis toolbox, the histo-cytometric multidimensional analysis pipeline (CytoMAP), which incorporates data clustering, positional correlation, dimensionality reduction, and 2D/3D region reconstruction to identify localized cellular networks and reveal features of tissue organization. We apply CytoMAP to study the microanatomy of innate immune subsets in murine lymph nodes (LNs) and reveal mutually exclusive segregation of migratory dendritic cells (DCs), regionalized compartmentalization of SIRPα− dermal DCs, and preferential association of resident DCs with select LN vasculature. The findings provide insights into the organization of myeloid cells in LNs and demonstrate that CytoMAP is a comprehensive analytics toolbox for revealing features of tissue organization in imaging datasets.
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•CytoMAP is a user-friendly, comprehensive platform for spatial analysis of tissues•Allows quantification of cellular positioning and global tissue structure•Enables exploration of cellular and tissue microenvironment heterogeneity•CytoMAP reveals fundamental features of myeloid cell organization in lymph nodes
Stoltzfus et al. present CytoMAP, a spatial analytics platform that incorporates diverse statistical and visualization modules for analysis of cellular positioning, cell-cell interactions, global tissue structure, and heterogeneity of tissue microenvironments. Exploration of myeloid cell localization in lymph nodes reveals fundamental positional relationships between dendritic cell subsets and local vasculature.
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