The Indian and Pacific Oceans surround the Maritime Continent (MC). Major modes of sea surface temperature variability in both oceans, including the Indian Ocean Dipole (IOD) and El Niño-Southern ...Oscillation (ENSO), can strongly affect precipitation on the MC. The prevalence of fires in the MC is closely associated with precipitation amount and terrestrial water storage in September and October. Precipitation and terrestrial water storage, which is a measurement of hydrological drought conditions, are significantly modulated by Indian Ocean Dipole (IOD) and El Niño events. We utilize long-term datasets to study the combined effects of ENSO and the IOD on MC precipitation during the past 100 years (1900-2019) and find that the reductions in MC precipitation and terrestrial water storage are more pronounced during years when El Niño and a positive phase of the IOD (pIOD) coincided. The combined negative effects are produced mainly through an enhanced reduction of upward motion over the MC. Coincident El Niño-pIOD events have occurred more frequently after 1965. However, climate models do not project a higher occurrence of coincident El Niño-pIOD events in a severely warming condition, implying that not the global warming but the natural variability might be the leading cause of this phenomenon.
Investigating the expression of RNAs that differ by short or single nucleotide sequences at a single-cell level in tissue has been limited by the sensitivity and specificity of in situ hybridization ...(ISH) techniques. Detection of short isoform-specific sequences requires RNA isolation for PCR analysis—an approach that loses the regional and cell-type-specific distribution of isoforms. Having the capability to distinguish the differential expression of RNA variants in tissue is critical because alterations in mRNA splicing and editing, as well as coding single nucleotide polymorphisms, have been associated with numerous cancers, neurological and psychiatric disorders. Here we introduce a novel highly sensitive single-probe colorimetric/fluorescent ISH approach that targets short exon/exon RNA splice junctions using single-pair oligonucleotide probes (~ 50 bp). We use this approach to investigate, with single-cell resolution, the expression of four transcripts encoding the neuregulin (NRG) receptor ErbB4 that differ by alternative splicing of exons encoding two juxtamembrane (JMa/JMb) and two cytoplasmic (CYT-1/CYT-2) domains that alter receptor stability and signaling modes, respectively. By comparing ErbB4 hybridization on sections from wild-type and ErbB4 knockout mice (missing exon 2), we initially demonstrate that single-pair probes provide the sensitivity and specificity to visualize and quantify the differential expression of ErbB4 isoforms. Using cell-type-specific GFP reporter mice, we go on to demonstrate that expression of ErbB4 isoforms differs between neurons and oligodendrocytes, and that this differential expression of ErbB4 isoforms is evolutionarily conserved to humans. This single-pair probe ISH approach, known as BaseScope, could serve as an invaluable diagnostic tool to detect alternative spliced isoforms, and potentially single base polymorphisms, associated with disease.
Genes encoding cell-surface proteins control nervous system development and are implicated in neurological disorders. These genes produce alternative mRNA isoforms which remain poorly characterized, ...impeding understanding of how disease-associated mutations cause pathology. Here we introduce a strategy to define complete portfolios of full-length isoforms encoded by individual genes. Applying this approach to neural cell-surface molecules, we identify thousands of unannotated isoforms expressed in retina and brain. By mass spectrometry we confirm expression of newly-discovered proteins on the cell surface in vivo. Remarkably, we discover that the major isoform of a retinal degeneration gene, CRB1, was previously overlooked. This CRB1 isoform is the only one expressed by photoreceptors, the affected cells in CRB1 disease. Using mouse mutants, we identify a function for this isoform at photoreceptor-glial junctions and demonstrate that loss of this isoform accelerates photoreceptor death. Therefore, our isoform identification strategy enables discovery of new gene functions relevant to disease.
During the past two decades, the Maritime Continent (MC) has experienced increased deforestation. Here we show, with ensemble idealized deforestation experiments, that the MC deforestation could ...potentially alter the complexity (i.e., event‐to‐event differences) of the El Niño‐Southern Oscillation (ENSO) in terms of its spatial pattern and temporal evolution. The deforestation model run increases the occurrences of the Central Pacific and multi‐year types of ENSO compared to the control experiments. This change in ENSO complexity can be attributed to MC's intensification of the subtropical ENSO dynamics, commonly known as the seasonal footprinting mechanism. The deforestation amplifies the mean state of the subtropical high over the northeastern Pacific, leading to an increased dominance of subtropical ENSO dynamics in determining the ENSO pattern and evolution. This idealized coupled climate modeling study suggests that MC deforestation has a potential to alter ENSO's complexity, making El Niño more complex and less predictable.
Plain Language Summary
This study examines how the deforestation in the maritime continent (MC) could induce a teleconnection that further alter the characteristics of El Niño‐Southern Oscillation (ENSO). Using the fully‐coupled Community Earth System Model, the researchers found that the sea level pressure over the North Pacific was strengthened in the idealized deforestation experiments. The anomalous high enhanced the air‐sea coupling in the subtropical northeastern Pacific which can spread into the tropical Pacific to affect ENSO properties. Climate model simulations indicate that deforestation has the potential to increases the occurrence of Central Pacific (CP) and multi‐year types of ENSO events compared to the control experiment. More CP and multi‐year ENSO events increase the challenge of predicting the characteristics of ENSO and its global impacts. This study unveils that the potential of MC deforestation in alter ENSO properties, which could have potentially serious implications for society.
Key Points
Maritime Continent deforestation has a potential to alter El Niño and La Niña complexities in spatial pattern and temporal evolutions
Deforestation can strengthen subtropical El Niño‐Southern Oscillation (ENSO) dynamics causing more Central Pacific and multi‐year ENSOs
The strengthened subtropical ENSO dynamics results from a deforestation‐induced intensification of the mean northeastern Pacific high
Aspirin is a prototypic cyclooxygenase inhibitor with a variety of beneficial effects on human health. It prevents age-related diseases and delays the aging process. Previous research has shown that ...aspirin might act through a dietary restriction-like mechanism to extend lifespan. To explore the mechanism of action of aspirin on aging, we determined the whole-genome expression profile of Caenorhabditis elegans treated with aspirin. Transcriptome analysis revealed the RNA levels of genes involved in metabolism were primarily increased. Reproduction has been reported to be associated with metabolism. We found that aspirin did not extend the lifespan or improve the heat stress resistance of germline mutants of glp-1. Furthermore, Oil Red O staining showed that aspirin treatment decreased lipid deposition and increased expression of lipid hydrolysis and fatty acid β-oxidation-related genes. The effect of germline ablation on lifespan was mainly mediated by DAF-12 and DAF-16. Next, we performed genetic analysis with a series of worm mutants and found that aspirin did not further extend the lifespans of daf-12 and daf-16 single mutants, glp-1;daf-12 and glp-1;daf-16 double mutants, or glp-1;daf-12;daf-16 triple mutants. The results suggest that aspirin increase metabolism and regulate germline signalling to activate downstream DAF-12 and DAF-16 to extend lifespan.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Stress disturbs the balance of the gut microbiota and stimulates inflammation-to-brain mechanisms. Moreover, stress leads to anxiety and depressive disorders.
displays distinct anti-inflammatory ...effects. However, no report has focused on the anxiolytic and antidepressant effects of
related to the gut microbiome and the inflammation on chronic restraint stress (CRS) in mice. We found that pretreatment with
increased the time spent in the center of the open field apparatus, increased the percentage of entries into the open arms of the elevated plus-maze (EPM) and the percentage of time spent in the open arms of the EPM, and decreased the immobility duration in the tail suspension test as well as the forced swimming test (FST). Moreover,
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markedly reduced the protein expression of interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), p-nuclear factor-kappa B (NF-κB) p65 and Iba1 and elevated brain derived neurotrophic factor (BDNF) expression in the hippocampus. We conclude that the anxiolytic and antidepressant effects of
are related to reducing inflammatory cytokines and rebalancing the gut microbiota.
In this paper, we study the existence of positive solution for the
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Energetic materials are a special and important kind of substance. Impact sensitivity, which refers to the vulnerability to explosion under external stimuli, measures the safety and reliability of an ...energetic material and is a critical property. Various efforts have been made to rationalize the impact sensitivity of different types of energetic materials. Since a chemical explosion is a chemical reaction dominated phenomenon, a comprehensive understanding of such explosive processes requires detailed information of chemical bonding and molecular interaction. Quantum chemistry provides a modern theory of chemical bonding and computational quantum chemistry is a powerful tool to investigate chemical phenomena. Even at the very beginning of computational quantum chemistry, researchers in the field of energetic materials have begun to apply quantum chemistry to explosive properties. In this paper we review the quantum chemistry studies on impact sensitivity and examine various quantum chemistry derived parameters used to rationalize the impact sensitivity ordering of various energetic materials.
PGC1α is a pleiotropic co-factor that affects angiogenesis, mitochondrial biogenesis, and oxidative muscle remodeling via its association with multiple transcription factors, including the master ...oxidative nuclear receptor ERRγ. To decipher their epistatic relationship, we explored ERRγ gain of function in muscle-specific PGC1α/β double-knockout (PKO) mice. ERRγ-driven transcriptional reprogramming largely rescues muscle damage and improves muscle function in PKO mice, inducing mitochondrial biogenesis, antioxidant defense, angiogenesis, and a glycolytic-to-oxidative fiber-type transformation independent of PGC1α/β. Furthermore, in combination with voluntary exercise, ERRγ gain of function largely restores mitochondrial energetic deficits in PKO muscle, resulting in a 5-fold increase in running performance. Thus, while PGC1s can interact with multiple transcription factors, these findings implicate ERRs as the major molecular target through which PGC1α/β regulates both innate and adaptive energy metabolism.
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•ERRγ rescues muscle damage in PGC1α/β-deficient muscle•Mitochondrial energetic defects in PGC1α/β-KO muscle are improved by ERRγ•ERRγ induces oxidative muscle remodeling and angiogenesis in PGC1α/β knockout mice•Exercise and ERRγ individually and cooperatively alleviate defects in PGC1 KO muscle
Fan et al. demonstrate that ERRγ improves mitochondrial energy metabolism in PGC1α/β-deficient muscle through its direct activation of target genes. Such ERRγ-induced effects are further boosted in combination with exercise training, suggesting ERRs are the major transcriptional modulator through which PGC1α/β regulates both innate and adaptive energy metabolism.
Sponge gourd is a versatile vegetable that can be used as a household cleaning product, industrial material and medicine, and possesses anti-human immunodeficiency virus activity. In the current ...study, a large dataset composed of 77,858 unigenes derived from the sponge gourd transcriptome was assembled. Of all unigenes, 52,113 (66.93 %) had significant similarity to known proteins from NCBI non-redundant, Swiss-Prot, Clusters of Orthologous Group and Kyoto Encyclopedia of Genes and Genomes databases. Based on these generated sequences, we identified 12,932 putative simple sequence repeats (SSRs) and successfully designed 8,523 high-quality SSR primer pairs. Six hundred and forty-one primer pairs were randomly selected to be verified among S1174 Luffa acutangula (L.) Roxb., 93075 L. cylindrica (L.) Roem. and their hybrid F1. The result showed that 494 (77.07 %) exhibited successful amplification, of which 201 (40.69 %) revealed polymorphism between S1174 and 93075. Fifty polymorphic expressed sequence tag (EST)-SSRs were used to genotype 60 sponge gourd accessions. Cluster analysis revealed two major clusters, one comprising 42 accessions of L. acutangula and the other 18 accessions belonging to L. cylindrica. Transferability of the 77 polymorphic EST-SSR markers was investigated in six other cucurbits: pumpkin, cucumber, wax gourd, bitter gourd, bottle gourd and chieh-qua, and the percentage of cross-genus amplification was 85.90, 83.33, 83.33, 79.49, 75.64 and 74.36 %, respectively. This indicated that EST-SSRs in sponge gourd could well be applied to other cucurbits. These transcriptome shotgun assembly sequences and EST-SSR data are of great value for the discovery of novel genes and for marker-assisted selection in sponge gourd.
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