Circular RNAs (circRNAs) contribute to the epigenetic modulation of pathological and physiological conditions. The understanding of the impact of circRNAs on generation of testicular inflammatory ...reactions is insufficient.
Our research adopted a poly I:C-triggered testicular inflammation murine model and cell assays.
Microarray data and quantitative evaluation revealed the elevation in the concentrations of Toll-like receptor 3 (TLR3), circRNA-9119, and retinoic acid inducible gene-I (RIG-I) and repression in the levels of miR-136 and miR-26a. Inhibition of circRNA-9119 expression impaired the inflammatory reactions in the separated Leydig and Sertoli cells subjected to poly I:C treatment. CircRNA-9119 suppressed the expression of miR-136 and miR-26a by acting as a microRNA sponge. miR-136 and miR-26a repressed the expression of RIG-I and TLR3 through the expected target region in Leydig and Sertoli cells in vitro. Inhibition of miR-136 and miR-26a expression, at least in part, restored the expression of inflammatory cytokines, which were inhibited upon circRNA-9119 expression silencing. Furthermore, the expression of circRNA-9119 was positively associated with RIG-I and TLR3 mRNA and protein levels. The expression of inflammatory genes triggered by poly I:C treatment was noticeably suppressed after RIG-I and TLR3 knockout.
Our results suggest that circRNA-9119 may serve as a competing endogenous RNA that insulated miR-136 and miR-26a and consequently defended RIG-I and TLR3 mRNAs against miR-26a/miR-136-mediated inhibition of testicular cells. Moreover, RIG-I and TLR3 contributed to the modulation of poly I:C-triggered inflammatory cytokine generation during orchitis in testicular cells.
This paper explores the characteristics and influencing factors of the spatial distribution of 889 national intangible cultural heritage sites in the Yellow River basin of China based on ArcGIS ...spatial analysis and Geodetector. The results show that the distribution of national intangible cultural heritage sites in the Yellow River basin is significantly different among provinces, and most of them are distributed in the central and western regions. From east to west, the density of intangible heritage in the Yellow River basin presents a triangular "one area and three points" pattern. All kinds of intangible cultural heritage in the Yellow River basin generally show the characteristic of aggregated distribution.Traditional skills, traditional medicine, traditional theatre, traditional music, and folklore have high levels of agglomeration, and traditional dance, Quyi, folk literature, and traditional fine arts have lower levels. The levels of traditional sports, entertainment and acrobatics have the lowest agglomeration. Among social and humanistic factors, highway density is the most influential indicator for the spatial distribution of national intangible cultural heritage in the Yellow River basin. GDP, population density and the urbanization level also have a great impact on the spatial distribution of national intangible cultural heritage in the Yellow River basin. Among geographical environmental factors, the river system and topography have a certain effect on the spatial distribution of national intangible cultural heritage in the Yellow River basin. Based on these facts, this paper finally discusses the specific path to protect and develop intangible cultural heritage in the Yellow River basin in the context of the newerato promote its creative protection and innovative development.
Reconstructing 3D face from sparse points is an ill-posed problem. While there already exits available solutions addressing this problem, to our knowledge, we propose a better-performed approach ...which can robustly reconstruct fine 3D face shape. Our method includes two modules: face model establishment based on probabilistic principal component analysis (PPCA) trained in an unsupervised manner to learn transformation between landmarks and point cloud in their low-dimensional representation, and 3D face reconstruction based on learned relation between them to reconstruct fine face shape. Overall, our method considers the probability of face shape and learns more useful information of 3D face shape. We compare our method with 3 typical and state-of-the-art methods on 2 datasets and the effectiveness of our method is demonstrated generally. Further experiments on datasets with noise of different intensities show the stability of our method.
The rapid development of the Internet has broadened the channels of dissemination of information, it has also led to the rapid and widespread propagation of rumors, which can have a serious negative ...impact socially. In this paper, an improved ISR-WV rumor propagation model integrating multichannels is proposed by considering the system’s time delay, and the influence of different channels of propagation on the dynamic process is further analyzed. Moreover, the basic reproduction number
R
0
, rumor-free equilibrium, and rumor-prevailing equilibrium, as well as their stability, are deduced. Then, an optimal control problem with pulse vaccination is designed. Finally, the validity of the model and theoretical results is verified by numerical simulations and a practical application. The results show that the rumor propagation threshold
R
0
is more sensitive to the rate of the propagation of the information base channel. The shorter the thinking time
τ
1
required for the ignorant to react after obtaining the information, the larger the final scale of propagation. Under this condition, the time delay
τ
2
spent by a spreader in producing a video is negatively related to the final scale of the propagation; conversely, a longer
τ
1
implies that the person tends to more cognizant, which can suppress the spread of rumors. Under this condition,
τ
2
has little effect on the final scale of propagation. In addition, the results also prove that timely implementation of the pulse vaccination control strategy of popular science education can effectively control the propagation of rumors and reduce their negative impact.
Activated osteoclasts cause excessive bone resorption, and disrupt bone homeostasis, leading to osteoporosis. The extracellular signal-regulated kinase (ERK) signaling is the classical pathway ...related to osteoclast differentiation, and mitochondrial reactive oxygen species are closely associated with the differentiation of osteoclasts. Myrislignan (MRL), a natural product derived from nutmeg, has multiple pharmacological activities; however, its therapeutic effect on osteoporosis is unclear. Here, we investigated whether MRL could inhibit osteoclastogenesis and bone mass loss in an ovariectomy mouse model by suppressing mitochondrial function and ERK signaling.
Tartrate-resistant and phosphatase (TRAP) and bone resorption assays were performed to observe the effect of MRL on osteoclastogenesis of bone marrow macrophages. MitoSOX RED and tetramethyl rhodamine methyl ester (TMRM) staining was performed to evaluate the inhibitory effect of MRL on mitochondria. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was performed to detect whether MRL suppressed the expression of osteoclast-specific genes. The impact of MRL on the protein involved in the mitogen-activated protein kinase (MAPK) and nuclear factor-κB pathways was evaluated using western blotting. In addition, a specific ERK agonist LM22B-10, was used to revalidate the inhibitory effect of MRL on ERK. Finally, we established an ovariectomy mouse model to assess the therapeutic effect of MRL on osteoporosis in vivo.
MRL inhibited osteoclast differentiation and the associated bone resorption, by significantly decreasing osteoclastic gene expression. Mechanistically, MRL inhibited the phosphorylation of ERK by suppressing the mitochondrial function, thereby downregulating the nuclear factor of activated T cells 1 (NFATc1) signaling. LM22B-10 treatment further verified the targeted inhibition effect of MRL on ERK. Microscopic computed tomographic and histologic analyses of the tibial tissue sections indicated that ovariectomized mice had lower bone mass and higher expression of ERK compared with normal controls. However, MRL treatment significantly reversed these effects, indicating the anti-osteoporosis effect of MRL.
We report for the first time that MRL inhibits ERK signaling by suppressing mitochondrial function, thereby ameliorating ovariectomy-induced osteoporosis. Our findings can provide a basis for the development of a novel therapeutic strategy for osteoporosis.
Gut microbiota is a primary driver of inflammation in the colon and is linked to early colorectal cancer (CRC) development. Thus, a novel and noninvasive microbiome-based model could promote ...screening in patients at average risk for CRC. Nevertheless, the relevance and effectiveness of microbial biomarkers for noninvasive CRC screening remains unclear, and researchers lack the data to distinguish CRC-related gut microbiome biomarkers from those of other common gastrointestinal (GI) diseases. Microbiome-based classification distinguishes patients with CRC from normal participants and excludes other CRC-relevant diseases (e.g., GI bleed, adenoma, bowel diseases, and postoperative). The area under the receiver operator characteristic curve (AUC) was 92.2%. Known associations with oral pathogenic features, benefits-generated features, and functional features of CRC were confirmed using the model. Our optimised prediction model was established using large-scale experimental population-based data and other sequence-based faecal microbial community data. This model can be used to identify the high-risk groups and has the potential to become a novel screening method for CRC biomarkers because of its low false-positive rate (FPR) and good stability.
Key points
•
A total of 5744 CRC and non-CRC large-scale faecal samples were sequenced, and a model was constructed for CRC discrimination on the basis of the relative abundance of taxonomic and functional features.
•
This model could identify high-risk groups and become a novel screening method for CRC biomarkers because of its low FPR and good stability.
•
The association relationship of oral pathogenic features, benefits-generated features, and functional features in CRC was confirmed by the study.
ObjectiveTo investigate whether and how simvastatin mediates protection from lethal sepsis, using a mouse model.MethodsSixty C57BL/6 mice were selected and divided into three groups (“control,” ...“model,” and “observation”; n = 20 mice per group). Mice in the model and observation groups underwent cecal ligation and puncture; mice in the observation group received simvastatin. After 24 hours of induced sepsis, serum concentrations of IL-6, TNF-α, IL-1, and IL-10 were measured by ELISA. Serum malondialdehyde (MDA) concentrations and serum superoxide dismutase (SOD) activities were quantified by radioimmunoassay.ResultsThe mean duration of survival of mice in the observation group was significantly longer than that of the model group. The serum concentrations of IL-6, TNF-α, IL-1, IL-10, and MDA were significantly higher in the observation group than in the control group. Serum SOD activities were significantly lower in the observation group than in the control group.ConclusionsSimvastatin can alleviate symptoms of sepsis in mice and improve their rates of survival. The mechanism of action of simvastatin may be mediated by inhibition of the systemic inflammatory response and oxidative stress.
With the development of science and technology, products are being updated more and more quickly. Therefore, the diffusion of product information can make people better choose products. It is very ...meaningful to study the competition and diffusion of multiple product information. In this paper, the dual product information diffusion model with preference was proposed based on the mean-field equation in complex networks. The dynamic of the model was analyzed by the analysis of Markov chains. According to the Monte Carlo simulation mechanism, the critical threshold of product information was obtained. The accuracy of the theory and model results is verified by computer simulation, and the scenarios in which the two products information are mutually promoted and mutually suppressed are simulated.
PDF
