The long noncoding RNA TINCR shows aberrant expression in human squamous carcinomas. However, its expression and function in gastric cancer remain unclear. We report that TINCR is strongly ...upregulated in human gastric carcinoma (GC), where it was found to contribute to oncogenesis and cancer progression. We also revealed that TINCR overexpression is induced by nuclear transcription factor SP1. Silencing TINCR expression inhibited cell proliferation, colony formation, tumorigenicity and apoptosis promotion, whereas TINCR overexpression promoted cell growth, as documented in the SGC7901 and BGC823 cell lines. Mechanistic analyses indicated that TINCR could bind to STAU1 (staufen1) protein, and influence KLF2 mRNA stability and expression, then KLF2 regulated cyclin-dependent kinase genes CDKN1A/P21 and CDKN2B/P15 transcription and expression, thereby affecting the proliferation and apoptosis of GC cells. Together, our findings suggest that TINCR contributes to the oncogenic potential of GC and may constitute a potential therapeutic target in this disease.
To explore the characteristics of Helicobacter pylori resistance in China and the association between antibiotic resistance and several clinical factors.
H. pylori strains were collected from ...patients in 13 provinces or cities in China between 2010 and 2016. Demographic data including type of disease, geographic area, age, gender and isolation year were collected to analyse their association with antibiotic resistance. Antibiotic resistance was detected using the Etest test and the Kirby-Bauer disc diffusion method.
H. pylori were successfully cultured from 1117 patients. The prevalence of metronidazole, clarithromycin (CLA), azithromycin, levofloxacin (LEV), moxifloxacin, amoxicillin (AMO), tetracycline and rifampicin resistance was 78.2, 22.1, 23.3, 19.2, 17.2, 3.4, 1.9 and 1.5%, respectively. No resistance to furazolidone was observed. The resistance rates to LEV and moxifloxacin were higher in strains isolated from patients with gastritis compared to those with duodenal ulcer and among women. Compared to patients ≥40 years old, younger patients exhibited lower resistance rates to CLA, azithromycin, LEV and moxifloxacin. The resistance rates to CLA and AMO were higher in strains isolated more recently, and we also found that the prevalence of resistance to metronidazole, CLA, azithromycin and AMO were significantly different among different regions of China.
The resistance rates to metronidazole, CLA and LEV were high in China. Patient age, gender, disease and location were associated with the resistance of H. pylori to some antibiotics. Furazolidone, AMO and tetracycline are better choices for H. pylori treatment in China.
Abstract In this research we employed layered double hydroxide nanoparticles (LDHs) to simultaneously deliver an anticancer drug 5-fluorouracil (5-FU) and Allstars Cell Death siRNA (CD-siRNA) for ...effective cancer treatment. The strategy takes advantage of the LDH anion exchange capacity to intercalate 5-FU into its interlayer spacing and load siRNA on the surface of LDH nanoparticles. LDH nanoparticles have been previously demonstrated as an effective cellular delivery system for 5-FU and siRNA separately in various investigations. More excitedly, the combination of CD-siRNA and anticancer drug 5-FU with the same LDH particles significantly enhanced cytotoxicity to three cancer cell lines, e.g. MCF-7, U2OS and HCT-116, compared to the single treatment with either CD-siRNA or 5-FU. This enhancement is probably a result of coordinate mitochondrial damage process. Thus, the strategy to co-deliver siRNA and an anticancer drug by LDHs has great potential to overcome the drug resistance and enhance cancer treatment.
Inspired by the relationship between the well-ordered architecture of aragonite crystals and biopolymers found in natural nacre, we present a facile strategy to construct large-scale ...organic/inorganic nacre-mimetics with hierarchical structure
via
a water-evaporation driven self-assembly process. We connect LAPONITE®-nanoclay platelets with each other using carboxymethyl cellulose, a cellulose derivative, thus creating thin, flexible films with a local brick-and-mortar architecture. The dried films show a pronounced resistance against tensile forces allowing for stronger thin films than nacre. In terms of functionalities, we report excellent glass-like transparency along with exceptional shape-persistent flame shielding. We also demonstrate that through metal ion-coordination we can further strengthen the interactions between the polymers and the nanoclays, and thus enhanced mechanical, and thermal properties as well as resistance against swelling and dissolution in aqueous environments. We believe that our simple pathway to fabricate such versatile polymer/clay nanocomposites can open avenues for inexpensive production of environmentally friendly, biomimetic materials in aerospace, wearable electrical devices, and in the food packaging industry.
Inspired by the relationship between the ordered architecture of aragonite crystals and biopolymers found in natural nacre, we present a facile strategy to construct organic/inorganic nanocomposites with hierarchical structure
via
a water-evaporation driven self-assembly process.
In this paper, a novel pulsating carrier signal injection strategy utilizing zero-sequence voltage is proposed for sensorless control of permanent-magnet synchronous machine (PMSM) drives. Different ...from the conventional zero-sequence carrier voltage sensing method using rotating signal injection in the stationary reference frame, the proposed pulsating injection is based on the estimated reference frame, which rotates anticlockwise at twice estimated rotor electrical angular speed. Then, the rotor position estimation is fulfilled via enabling the zero-sequence carrier voltage to zero. Compared with conventional rotating injection using zero-sequence voltage, the proposed strategy is simpler for signal demodulation and more robust to signal processing delays due to the fact that it is amplitude modulated by machine saliency and phase shifts of saliency position due to signal processing delays are intrinsically cancelled, which is the same as the classical pulsating injection in the estimated synchronous reference frame with carrier current sensing. Therefore, the proposed method can combine the synergies of zero-sequence method (i.e., high bandwidth and stability) and pulsating injection (i.e., increased accuracy and fast dynamic response). Furthermore, the cross-coupling magnetic saturation effects on zero-sequence voltage sensing-based sensorless control are discussed in detail. All the theoretical analyses are validated by experiments on a laboratory surface-mounted PM (SPM) machine.
A consequent tendency toward high-performance quantum information processing is to develop the fully integrated photonic chip. Here, we report the on-chip generation and manipulation of entangled ...photons based on reconfigurable lithium-niobate waveguide circuits. By introducing a periodically poled structure into the waveguide circuits, two individual photon-pair sources with a controllable electro-optic phase shift are produced within a Hong-Ou-Mandel interferometer, resulting in a deterministically separated identical photon pair. The state is characterized by 92.9±0.9% visibility Hong-Ou-Mandel interference. The photon flux reaches ∼1.4×10(7) pairs nm-1 mW-1. The whole chip is designed to contain nine similar units to produce identical photon pairs spanning the telecom C and L band by the flexible engineering of nonlinearity. Our work presents a scenario for on-chip engineering of different photon sources and paves the way to fully integrated quantum technologies.
In this paper, a novel square-wave-type carrier signal injection method using zero-sequence voltage is proposed for sensorless control of permanent-magnet (PM) synchronous machine drives. Different ...from the conventional square-wave-type injection methods employed in the stationary reference frame and estimated synchronous reference frame, the proposed square-wave injection is performed on the estimated reference frame, which rotates anticlockwise at twice estimated rotor electrical angular speed. Compared to the conventional square-wave methods with carrier current sensing, the proposed strategy using zero-sequence voltage has two main advantages, 1) the amplitude of the resultant carrier response is not related to the injection frequency, and 2) the carrier response does not require differentiation calculation for rotor position estimation. Therefore, the proposed method is more robust and also much simpler for signal demodulation, which can combine the synergies of zero-sequence method and square-wave injection (i.e., increased accuracy, bandwidths, and stability, etc.). Finally, all the theoretical analyses are validated by experiments on a laboratory PM machine.
H
2
O and O
2
are believed to be key factors that influence the structural stability of black phosphorus (BP) in ambient conditions. In this work, the interactions of H
2
O and/or O
2
with BP are ...investigated using first-principles calculations. The results indicate that water molecules prefer to adsorb on the BP surface and form a six-member water ring. The dissociation barrier of O
2
is significantly reduced in the presence of H
2
O, which dramatically promotes the degradation of BP. Moreover, the introduction of O
2
also facilitates the adsorption of water on the surface. The effects of H
2
O and/or O
2
on the quasiparticle band gap and exciton binding energy of BP are also investigated. The results suggest that water adsorption has only a slight influence on the electronic properties and exciton binding energy, while O
2
adsorption causes obvious changes in the properties of BP, which results in a direct-to-indirect band gap transition in BP.
The synergetic effect of O
2
and H
2
O during the oxidation of black phosphorus (BP) at the atomic level is revealed, and the effects of H
2
O and/or O
2
on the properties of BP are also investigated.
Prior data indicate similar outcomes after transplants from human leukocyte antigen (HLA)-haplotype-matched relatives, HLA-identical siblings and HLA-matched unrelated donors. We used our prospective ...data set to answer a clinically important question: who is the best donor for a person with acute leukaemia transplanted in first complete remission. Patients were randomly divided into training (n=611) and validation (n=588) sets. A total of 1199 consecutive subjects received a transplant from an HLA-haplotype-matched relative using granulocyte colony-stimulating factor and anti-thymocyte globulin (n=685) or an HLA-identical sibling (n=514); 3-year leukaemia-free survivals (LFSs) were 75 and 74% (P=0.95), respectively. The multivariate model identified three major risk factors for transplant-related mortality (TRM): older donor/recipient age, female-to-male transplants and donor-recipient ABO major-mismatch transplants. A risk score was developed based on these three features. TRMs were 8%, 15% and 31% for subjects with scores of 0-1, 2 and 3, respectively, (P<0.001). Three-year LFSs were 78%, 74% and 58%, respectively, (P=0.003). The risk score was validated in an independent cohort. In conclusion, our data confirm donor source is not significantly correlated with transplant outcomes. Selection of the best donor needs to consider donor-recipient age, matching for gender and ABO incompatibility among persons with acute leukaemia receiving related transplants under our transplant modality.
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