Long noncoding RNA (lncRNA) plays crucial roles in various biological processes of different cancers, especially acting as a competing endogenous RNA (ceRNA). However, the role of lncRNA-mediated ...ceRNA in Wilms tumor (WT), which is the most common malignant kidney cancer in children, remains unknown. In present study, RNA sequence profiles and clinical data of 125 patients with WT consisting of 119 tumor and 6 normal tissues from Therapeutically Applicable Research To Generate Effective Treatments database were analyzed. A total of 1833 lncRNAs, 156 microRNAs (miRNAs), and 3443 messenger RNAs (mRNAs) were identified as differentially expressed (DE) using “DESeq2” package. The lncRNA-miRNA-mRNA ceRNA regulatory network involving 748 DElncRNAs, 33 DEmiRNAs, and 189 DEmRNAs was constructed based on miRcode, Targetscan, miRTarBase, and miRDB database. Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that DEmRNAs were mainly enriched in cell proliferation-related processes and tumor-related pathways, respectively, and 13 hub genes were identified by a protein–protein interaction network. Survival analysis detected 48 lncRNAs, 7 miRNAs, and 16 mRNAs to have significant impact on the overall survival of patients with WT. Additionally, we found that 6 DElncRNAs with potential prognostic value were correlated with tumor stage (DENND5B-AS1) and histologic classification (TMPO-AS1, RP3-523K23.2, RP11-598F7.3, LAMP5-AS1, and AC013275.2) of patients with WT. Our research provides a great insight into understanding the molecular mechanism underlying occurrence and progression of WT, as well as the potential to develop targeted therapies and prognostic biomarkers.
Abstract Coronary collateral circulation is an alternative source of blood supply to the myocardium in the presence of chronic total coronary occlusion (CTO). C-X-C motif chemokine receptor 4 (CXCR4) ...signaling usually contributes to neovascularization. Here, we investigate the relationship between CXCR4 levels in peripheral blood CD34+ cells and the formation of angiographic coronary collaterals, and determine the risk factors that affect CXCR4 expression in patients with CTO. Demographic, biochemical, and angiographic variables were collected from 324 CTO patients and 90 negative controls. The presence and extent of collaterals were scored according to the Rentrop scoring system (Rentrop’s). CXCR4 levels and plasma biochemical factors were detected. Clinical outcomes were collected during a 12 month follow up. Results show that low (Rentrop’s 0 or 1) and high (Rentrop’s of 2 or 3) coronary collateralizations were detected in 183 and 141 patients, respectively. The Rentrop scores were positively correlated with CXCR4 levels in CTO patients. Patients with low CXCR4 expression exhibited worse clinical outcomes compared with those with high CXCR4 expression. Univariate correlation analysis revealed that age of ≥65 years, female, diabetes, increased plasma level of high-sensitivity C-reactive protein (hs-CRP) and N-terminal brain-typenatriuretic peptide (NT-proBNP) were associated with low CXCR4 levels. In conclusion, CXCR4 levels were positively correlated with the presence and extent of angiographic coronary collaterals in patients with CTO. Elder age, female, diabetes, increased plasma level of hs-CRP and NT-proBNP may be risk factors of CXCR4 expression.
Abstract Background and Purpose: Patients with small (< 5 mm) unruptured intracranial aneurysms (UIAs) are at risk of subarachnoid hemorrhage, but risk assessment of them remains controversy in daily ...clinical practice. We aimed to identify the risk factors of aneurysmal rupture in these patients. Methods We retrospectively analyzed consecutive patients with small UIAs who were admitted to our center between February 2009 and December 2014. The enrolled patients were divided into the ruptured and unruptured groups. The risk factors for aneurysmal rupture were determined using multivariate logistic regression analysis. Results A total of 548 patients with 618 small intracranial aneurysms (267 ruptured and 351 unruptured) were included. Univariate analysis revealed that rupture of small aneurysms was related to sex, age, smoking, hypertension, aspect ratio, size ratio, irregular shape, aneurysm width, height, and neck diameter, and location at bifurcation or posterior circulation. Multivariate logistic regression revealed that rupture was associated with bifurcation location (odds ratio OR, 5.409, 95% confidence interval CI, 3.656-8.001, P < 0.001), size ratio (OR, 3.092, 95% CI, 2.002-4.774, P < 0.001), location (OR, 2.624, 95% CI, 1.428-4.824, P = 0.002), hypertension (OR, 1.698, 95% CI, 1.1140-2.527, P = 0.009), and age at diagnosis of UIA (OR, 1.826, 95% CI, 1.225-2.723, P = 0.003). Conclusion This study revealed that 70.4% of small ruptured intracranial aneurysms (<5 mm) located at parent artery bifurcations, and bifurcation location was a significant independent factor for the risk of rupture of small UIAs (<5 mm) . Prophylactic treatment may should be recommended for small UIAs in this location.
Background Isolated dissecting aneurysms of the posterior inferior cerebellar artery (PICA) are rare lesions, which carry high risk of rebleeding and mortality. However, the existing literature ...concerning predictors of outcome after endovascular treatment is limited and controversial. Our present study retrospectively reviewed and analyzed the clinical outcome of endovascular treatment–ruptured PICA-dissecting aneurysms and explored the predictors of outcome. Methods We retrospectively reviewed 17 consecutive patients with ruptured PICA dissecting aneurysms that underwent endovascular treatment from January 2003 to January 2014. Nine patients underwent selective coiling, whereas 7 patients underwent parent artery occlusion and 1 patient underwent stent-assisted coiling. Follow-up outcomes were evaluated using the modified Rankin Scale. The clinical outcomes of patients were categorized as favorable (modified Rankin Scale mRS score 0-1) or unfavorable (mRS score 2-6). Results Favorable outcomes (mRS score 0-1) were obtained in 13 of 17 patients. Post-treatment recurrence occurred in 1 patient with selective coiling in the 15-month follow-up, and the patient received stent-assisted coiling. The only patients with stent-assisted coiling developed PICA occlusion during follow-up. Aneurysm located in distal segment usually presented with intraventricular hemorrhage ( P = .015). Hypertension, coexisting hydrocephalus, and time to operation (latter than 2 weeks) were associated with unfavorable outcome. Conclusions Endovascular treatment of isolated dissecting aneurysm of PICA had excellent clinical outcomes, hypertension, coexisting hydrocephalus, and time to operation (latter than 2 weeks), which were associated with unfavorable outcome. Long-term follow-ups are necessary to provide stronger conclusions.
Background: Recently, considerable evidence pointed out monocyte to high-density lipoprotein ratio (MHR) is highly related to inflammatory related diseases. We aim to explore the level of MHR in ...acute aortic dissection (AAD) patients and determine whether MHR can be a novel diagnostic marker of AAD.
Research design and methods: A total of 228 subjects including 128 AAD patients and 110 healthy control were enrolled. MHR levels and other serum samples were obtained at admission.
Results: The baseline MHR levels were significantly higher in patients with AAD (p < 0.0001). A cutoff value of MHR >0.37 was associated with a sensitivity of 86.70% and a specificity of 93.60% for AAD. MHR levels were positively correlated with the time from symptom onset (R
2
= 0.0318, p = 0.0003). Additionally, the area under the curve (AUC) was increased to 0.979 in patients whose time from onset of symptoms >24 h, with a sensitivity of 98.04% and a specificity of 93.64%. Multivariate logistic regression demonstrated that MHR levels, history of hypertension, and coronary artery disease (CHD) emerged as independent predictors of AAD.
Expert Opinion: MHR has a high diagnostic value in AAD patients, especially in those whose time from onset of symptoms >24 h.
Background Management of intracranial basilar dissecting aneurysms has been controversial and challenging, and surgical and conservative treatments usually have a bad prognosis. Our study aimed at ...evaluating the outcomes of endovascular treatment for these lesions and exploring the predictors of favorable outcome. Methods We retrospectively reviewed 50 consecutive patients with basilar dissecting aneurysms from January 2006 to January 2013. Twenty-four patients underwent stent-assisted coiling whereas 26 patients underwent conservative treatment. Follow-up outcomes were evaluated using modified Rankin Scale (mRS) score. Results Of the patients treated with stent-assisted coiling, 20 patients had a favorable outcome (mRS score, 0-1), post-treatment recurrence occurred in 3 patients, 1 had rebleeding, and 2 had no rebleeding. Of the patients treated with conservative therapy (observation or anticoagulation), 10 patients had an unfavorable outcome, 2 patients with ruptured aneurysms developed rebleeding, and 8 patients had poor outcome because of infarct progression. Stent-assisted coiling group had a more favorable outcome than the conservatively treated group (83.3% versus 55.2%, P = .019). Initial complete obliteration was related to the favorable outcome in endovascular-treated group ( P = .042). Stent placement was the only independent predictor of favorable outcome in the logistic regression analysis ( P = .030; odds ratio = 5.828; 95% confidence interval, 1.192-28.503). Conclusions Patients with basilar artery dissecting aneurysms treated with stent-assisted coiling had a more favorable outcome than the conservatively treated patients. Stent placement and initial complete occlusion were the favorable factors in patients with basilar dissecting aneurysm.
Abstract Objective Unruptured paraclinoid aneurysms are often asymptomatic, and endovascular coiling is the main treatment. However, endovascular treatment of these lesions still leads to ...neurological complications. We aimed to identify predictors of neurological complications in these lesions. Methods We retrospectively analyzed patients with unruptured paraclinoid aneurysms who were treated with endovascular coiling between January 2014 and December 2015. A neurological complication was defined as any transient or permanent increase in the modified Rankin Scale score after aneurysm embolization. Univariate and mulitivariate logistic regression analyses were performed to assess the risk factors of neurological complications. Results Of the 443 unruptured paraclinoid aneurysms that were included in this study, the incidence of neurological complications was 5.2%. Neurological complications were highly correlated with hypertension (odds ratio OR, 3.147; 95% confidence interval CI, 1.217-8.138; P =0.018), cerebral ischemic comorbidities (OR, 3.396; 95% CI, 1.378-8.374; P =0.008), and aneurysm size (OR, 7.714; 95% CI, 1.784-31.635; P <0.001), and irregular shape (OR, 3.157; 95% CI, 1.239-8.043; P= 0.016) in the univariate analysis. Cerebral ischemic comorbidities (OR, 2.837, 95% CI, 1.070-7.523; P =0.036) and aneurysm size as dichotomous variables (OR, 7.557; 95% CI, 2.975-19.198; P <0.001) were strongly correlated with neurological complications in the final adjusted multivariate logistic analysis. Conclusions Unruptured paraclinoid aneurysms after endovascular treatments had 5.2% of neurological complications. Cerebral ischemic comorbidities and aneurysm size were predictors of neurological complications.
Abstract Background Recent studies have reported the use of stent-assisted coiling (SAC) for wide-necked aneurysms in patients with acute subarachnoid hemorrhage (SAH). This study attempted to ...determine whether it is safe and efficient to use SAC for wide-necked aneurysms during post-SAH days 4-10. Methods We reviewed 126 consecutive patients with ruptured wide-necked aneurysms who underwent SAC. The patients were classified into the early cohort (SAC was performed within post-SAH days 0-3) and late cohort (SAC was performed within post-SAH days 4-10). Intergroup differences in patients’ demographics, aneurysm features, angiographic vasospasm, periprocedural complications, and clinical and angiographic outcomes were analyzed. Results Of the 126 study patients, there were 70 (55.6%) in the early cohort and 56 (44.4%) in the late cohort. Angiographic vasospasm was significantly more likely to occur in patients treated in the late cohort (p<0.05). No difference (p>0.05) in age, gender, hypertension, dichotomized Fisher grade, dichotomized Hunt-Hess score, aneurysm features, aneurysm location, or periprocedural complications was found between the cohorts. We followed up 112 patients, in whom clinical and angiographic results showed no statistical significance (p>0.05) between the two cohorts. When the patients were divided based on the dichotomized modified Rankin Scale into the “good outcome” and “poor outcome” groups at 6 months follow-up, higher Hunt-Hess scores (p<0.001) were the only independent risk factors for poor outcome on multivariate logistic regression analysis. Conclusion Patients with ruptured wide-necked aneurysms treated on post-hemorrhage days 4-10 did not appear to have worse outcomes compared to patients treated on post-hemorrhage days 0-3.
Lung cancer is a common cause of cancer-related death. The link between risk of lung cancer susceptibility and genetic polymorphisms in metabolic enzymes is well documented. In this study, the ...relationships between lung cancer susceptibility and polymorphisms in the phase I metabolic enzyme genes
CYP1A1
,
CYP2D6,
and
CYP2A6
were investigated. Genomic DNA was isolated from the peripheral blood of 201 healthy controls and 168 lung carcinoma patients from the Han ethnic group of Hunan Province in Central South China. Polymorphisms of the investigated genes were analyzed using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and two-step allelic-specific PCR assays. No significant differences were found between the frequencies in cases and controls for the genotypes wild-type (WW), heterozygous mutant, or homozygous mutant; for
CYP1A1
or
CYP2D6
; or for the genotypes WW, heterozygous deletion, or null genotype for
CYP2A6
. The three-locus model (
CYP2A6/CYP1A1/CYP2D6
) had a maximum test sample accuracy that was significant (
P
< 0.001) with a cross-validation consistency of 10. These results indicated that the three-order interaction of
CYP2A6
,
CYP1A1,
and
CYP2D6
polymorphisms might increase genetic susceptibility to lung cancer. We report the involvement of a three-order interaction between
CYP1A1, CYP2A6,
and
CYP2D6
polymorphisms in lung cancer risk in people in Central South China, although no relationship between lung cancer risk and individual gene polymorphisms was found.
This study was designed to investigate the effects of local delivery of bone marrow mesenchymal stem cells (BMMSCs) with or without osterix (OSX) gene transfected on bone regeneration in the ...distracted zone using a rabbit model of mandibular lengthening. Fifty-four New Zealand white rabbits underwent osteodistraction of the left mandible and were then randomly divided into group A, group B, and group C (n = 18 for each group). At the end of distraction BMMSCs transfected with OSX, autologous BMMSCs and physiological saline were injected into the distraction gaps in groups A, B, and C, respectively. Nine animals from each group were humanely killed at 2 and 6 weeks after completion of distraction. The distracted mandibles were harvested and processed for radiographic, histological, and immunohistochemical examination. Excellent bone formation in the distracted callus was observed in group A and group B; the former showed better bone formation and highest bone mineral density (BMD), thickness of new trabeculae (TNT, mm) and volumes of the newly formed bone area (NBV) in the distraction zones. Group C animals showed poor bone formation in the distracted callus when compared with groups A and B. Positive immunostaining of bone sialoprotein (BSP) was observed in the distracted callus in all groups; however, BSP expression was much stronger in group A than in groups B and C. The results of this study suggest transplantation of BMMSCs can promote bone formation in DO; OSX-mediated ex vivo gene therapy was more effective during bone deposition and callus formation in distraction osteogenesis.
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