Indiscriminate use of chemical fertilizers in the agricultural production systems to keep pace with the food and nutritional demand of the galloping population had an adverse impact on ecosystem ...services and environmental quality. Hence, an alternative mechanism is to be developed to enhance farm production and environmental sustainability. A nanohybrid construct like nanofertilizers (NFs) is an excellent alternative to overcome the negative impact of traditional chemical fertilizers. The NFs provide smart nutrient delivery to the plants and proves their efficacy in terms of crop productivity and environmental sustainability over bulky chemical fertilizers. Plants can absorb NFs by foliage or roots depending upon the application methods and properties of the particles. NFs enhance the biotic and abiotic stresses tolerance in plants. It reduces the production cost and mitigates the environmental footprint. Multitude benefits of the NFs open new vistas towards sustainable agriculture and climate change mitigation. Although supra-optimal doses of NFs have a detrimental effect on crop growth, soil health, and environmental outcomes. The extensive release of NFs into the environment and food chain may pose a risk to human health, hence, need careful assessment. Thus, a thorough review on the role of different NFs and their impact on crop growth, productivity, soil, and environmental quality is required, which would be helpful for the research of sustainable agriculture.
Display omitted
•Nanofertilizers (NFs) are the best alternative to traditional chemical fertilizers.•Nutrients use efficiency of NFs is higher than the conventional chemical fertilizers.•NFs can increase the tolerance of plants against biotic and abiotic stresses.•Supra optimal dose of NFs had a negative impact on crops, soil, and the environment.
Oxidative stress is a normal phenomenon in the body. Under normal conditions, the physiologically important intracellular levels of reactive oxygen species (ROS) are maintained at low levels by ...various enzyme systems participating in the in vivo redox homeostasis. Therefore, oxidative stress can also be viewed as an imbalance between the prooxidants and antioxidants in the body. For the last two decades, oxidative stress has been one of the most burning topics among the biological researchers all over the world. Several reasons can be assigned to justify its importance: knowledge about reactive oxygen and nitrogen species production and metabolism; identification of biomarkers for oxidative damage; evidence relating manifestation of chronic and some acute health problems to oxidative stress; identification of various dietary antioxidants present in plant foods as bioactive molecules; and so on. This review discusses the importance of oxidative stress in the body growth and development as well as proteomic and genomic evidences of its relationship with disease development, incidence of malignancies and autoimmune disorders, increased susceptibility to bacterial, viral, and parasitic diseases, and an interplay with prooxidants and antioxidants for maintaining a sound health, which would be helpful in enhancing the knowledge of any biochemist, pathophysiologist, or medical personnel regarding this important issue.
Environmental biotechnology is the use of biotechnology to develop and regulate biological systems for the remediation of environmental contamination. Nature has gifted ample material for remediation ...of its resources, among which chitosan is one of the most important and largely available biomaterial globally. Chitosan is a biopolymer obtained by deacetylation of chitin extracted from marine waste and its applications from drug delivery to food additives are broadly available. Chitosan exhibit several properties such as availability, low cost, high biocompatibility, and biodegradability. These properties make it biologically and chemically acceptable for use in various fields. Due to some limitations of pure chitosan, there has been a growing interest in modifying the chitosan in order to improve the original properties and widen the applications of pure phase chitosan. Various modified forms of chitosan and their associated applications are reviewed here with emphasis on their use in environmental remediation. The demand of chitosan in the global industrial market is growing which is briefly explained in this paper. Chitosan is used for water purification since a long time and still progress is going on for making it more efficient in the removal process. It can be used as a flocculent and coagulant, as an adsorbent for removing the contaminants like heavy metals, dyes, pesticides, antibiotics, biological contaminants from wastewater. Soil remediation using chitosan material is explained in this review. Various other applications such as drug delivery, food additives, tissue engineering are thoroughly reviewed.
•Chitosan exhibit numerous properties which are used in ample of applications.•Biotechnology develops natural systems for environmental remediation.•Hydroxyl and amine groups on chitosan responsible for different modifications.•Application of chitosan for environmental remediation has been compiled here.
B-cell receptor (BCR) signals play a critical role in the pathogenesis of chronic lymphocytic leukemia (CLL), but their role in regulating CLL cell proliferation has still not been firmly ...established. Unlike normal B cells, CLL cells do not proliferate in vitro upon engagement of the BCR, suggesting that CLL cell proliferation is regulated by other signals from the microenvironment, such as those provided by Toll-like receptors or T cells. Here, we report that BCR engagement of human and murine CLL cells induces several positive regulators of the cell cycle, but simultaneously induces the negative regulators CDKN1A, CDKN2A, and CDKN2B, which block cell-cycle progression. We further show that introduction of genetic lesions that downregulate these cell-cycle inhibitors, such as inactivating lesions in CDKN2A, CDKN2B, and the CDKN1A regulator TP53, leads to more aggressive disease in a murine in vivo CLL model and spontaneous proliferation in vitro that is BCR dependent but independent of costimulatory signals. Importantly, inactivating lesions in CDKN2A, CDKN2B, and TP53 frequently co-occur in Richter syndrome (RS), and BCR stimulation of human RS cells with such lesions is sufficient to induce proliferation. We also show that tumor cells with combined TP53 and CDKN2A/2B abnormalities remain sensitive to BCR-inhibitor treatment and are synergistically sensitive to the combination of a BCR and cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitor both in vitro and in vivo. These data provide evidence that BCR signals are directly involved in driving CLL cell proliferation and reveal a novel mechanism of Richter transformation.
•Biallelic inactivation of TP53 and CDKN2A/CDKN2B allows for BCR-dependent/costimulatory signal-independent proliferation of CLL cells.•BCR and CDK4/6 inhibitors display synergistic activity against RS models with genetic lesions in TP53 and CDKN2A/CDKN2B.
Display omitted
Soil – water pollution resulting from anthropogenic activities is a growing concern internationally. Effective monitoring techniques play a crucial role in the detection, prevention, and remediation ...of polluted sites. Current pollution monitoring practices in many geographical locations are primarily based on physico–chemical assessments which do not always reflect the potential toxicity of contaminant ‘cocktails’ and harmful chemicals not screened for routinely. Biomonitoring provides a range of sensitive techniques to characterise the eco–toxicological effects of chemical contamination. The bioavailability of contaminants, in addition to their effects on organisms at the molecular, cellular, individual, and community level allows the characterisation of the overall health status of polluted sites and ecosystems. Quantifying bioaccumulation, changes to community structure, faunal morphology, behavioural, and biochemical responses are standard procedures employed in biomonitoring studies in many High-Income Countries (HICs). This review highlights the need to integrate biomonitoring tools alongside physico–chemical monitoring techniques by using ‘effect–based’ tools to provide more holistic information on the ecological impairment of soil-water systems. This paper considers the wider implementation of biomonitoring methods in Low to Middle Income Countries (LMICs) and their significance in pollution investigations and proposes an integrated monitoring framework that can identify toxicity drivers by utilising ‘effect–based’ and ‘risk–based’ monitoring approaches.
Display omitted
•Low to Middle Income Countries require robust and cost-effective monitoring methods.•Pollution monitoring practises can be improved by incorporating biomonitoring.•Biomonitoring bridges information between chemical analysis and ecosystem health.•Biomonitoring allows the comparison of effects in various ecosystem compartments.•A framework for implementation of integrated pollution monitoring is proposed.
Earlier we have reported mercury-induced alterations in functional dynamics of buck spermatozoa through free radicals-mediated oxidative stress and spontaneous acrosome reaction. Based on our earlier ...findings, we aimed to investigate the effect of mercury exposure on motility, kinematic patterns, DNA damage, apoptosis and ultra-structural alterations in goat spermatozoa following in vitro exposure to different concentrations (0.031-1.25 µg/ml) of mercuric chloride for 15 min and 3 h. Following exposure of sperm cells to 0.031 µg/ml of mercuric chloride for 3 h, livability and motility of sperms was significantly reduced along with altered kinematic patterns, significant increase in per cent necrotic sperm cells and number of cells showing DNA damage; and this effect was dose- and time-dependent. Contrary to up-regulation of Bax gene after 3 h in control group, there was significant increase in expression of Bcl-2 in mercury-treated groups. Transmission electron microscopy studies revealed rifts and nicks in plasma and acrosomal membrane, mitochondrial sheath, and collapsed mitochondria with loss of helical organization of mitochondria in the middle piece of spermatozoa. Our findings evidently suggest that mercury induces necrosis instead of apoptosis and targets the membrane, acrosome, mid piece of sperms; and the damage to mitochondria seems to be responsible for alterations in functional and kinematic attributes of spermatozoa.
The glucosinolate breakdown product indole‐3‐carbinol functions in cruciferous vegetables as a protective agent against foraging insects. While the toxic and deterrent effects of glucosinolate ...breakdown on herbivores and pathogens have been studied extensively, the secondary responses that are induced in the plant by indole‐3‐carbinol remain relatively uninvestigated. Here we examined the hypothesis that indole‐3‐carbinol plays a role in influencing plant growth and development by manipulating auxin signaling. We show that indole‐3‐carbinol rapidly and reversibly inhibits root elongation in a dose‐dependent manner, and that this inhibition is accompanied by a loss of auxin activity in the root meristem. A direct interaction between indole‐3‐carbinol and the auxin perception machinery was suggested, as application of indole‐3‐carbinol rescues auxin‐induced root phenotypes. In vitro and yeast‐based protein interaction studies showed that indole‐3‐carbinol perturbs the auxin‐dependent interaction of Transport Inhibitor Response (TIR1) with auxin/3‐indoleacetic acid (Aux/IAAs) proteins, further supporting the possibility that indole‐3‐carbinol acts as an auxin antagonist. The results indicate that chemicals whose production is induced by herbivory, such as indole‐3‐carbinol, function not only to repel herbivores, but also as signaling molecules that directly compete with auxin to fine tune plant growth and development.
PDF
