Epidemiologic and preclinical studieshave shown that marine n‐3 polyunsaturated fatty acids (n‐3 PUFAs) elicit promising chemoprevention against breast cancer. Docosahexaenoic acid monoglyceride ...(MAG‐DHA), a docosahexaenoic acid sn‐1‐monoacylglycerol does not required pancreatic lipase to be absorbed, eliciting a better bioavailability when compared with other formulations such as DHA‐free fatty acid, DHA‐triglycerol, or DHA‐ethyl ester. However, the anticancer actions and underlying mechanisms of MAG‐DHA on breast cancer remain to be assessed. In this study, MAG‐DHA induced significant growth inhibition in MCF‐7 and MDA‐MB‐231 breast cancer cells in a dose‐dependent manner. MAG‐DHA treatment (80 µM) led to 83.8 and 94.3% growth inhibition between MCF‐7 and MDA‐MB‐231 cells, respectively. MAG‐DHA‐induced growth inhibition was tightly associated with apoptosis, as evidenced by increased active forms of caspase‐3, poly (ADP‐ribose) polymerase (PARP) and caspase‐12. In particular, MAG‐DHA‐induced apoptosis was triggered by oxidative stress‐mediated endoplasmic reticulum (ER) stress, as evidenced by activation of the PERK‐eIF2α pathway in ER. MAG‐DHA treatment also strongly suppressed the growth of E0771 murine breast cancer xenografts, significant differences of tumor volume were found between MAG‐DHA group (0.271 cm3) and control group (0.875 cm3) after 15 daily MAG‐DHA treatments. The in vitro antibreast cancer mechanism of MAG‐DHA was supported by the in vivo xenograft model. In addition, MAG‐DHA‐induced ER stress concomitantly triggered autophagy in these cancer cells, and the induction of autophagy suppressed its ability to induce apoptotic cell death. Our data suggested that MAG‐DHA as dietary supplement, in combination with autophagy inhibitors may be a useful therapeutic strategy in treating breast cancer.
We report here the purification of a novel metal-binding protein from
(
MT-1) by gel and ion-exchange chromatography. SDS-PAGE and MALDI-TOF analyses demonstrated that isolated
MT-1 was of high ...purity with a molecular weight of 12.4 kDa. The fluorescence response to SBD-F derivatives revealed that
MT-1 contained a large number of sulfhydryl groups, which is a general property of metallothioneins. Zn and Cu metal stoichiometries for
MT-1 were 3.97:1 and 0.55:1, respectively. The proportion of cysteine (Cys) residues in the amino acid composition was 32.69%, and aromatic amino acids were absent. The peptide sequence coverage with
calmodulin (accession AOA3S8FSK5) was 60%. Infrared spectroscopy of
MT-1 revealed two obvious peaks at absorption frequencies for the amide I band and the amide II band. CD spectra revealed that the secondary structure was mainly composed of random coil (57.6%) and β-sheet (39.9%). An evaluation of in vitro antioxidant activity revealed that isolated
MT-1 has strong reducing activities, exhibiting scavenging rates for DPPH and OH of 77.8% and 75.8%, respectively (IC
values 0.57 mg/mL and 1.1 mg/mL).
MT-1 may be used as a functional additive in cosmetics, health foods, and medical products, as well as a reference material for quantitative analysis of metallothionein in such products.
We perform a series of high-pressure synchrotron x-ray diffraction （XRD） and resistance measurements on the Weyl semimetal NbAs. The crystal structure remains stable up to 26 GPa according to the ...powder XRD data. The resistance of NbAs single crystal increases monotonically with pressure at low temperature. Up to 20 GPa, no superconducting transition is observed down to 0.3 K. These results show that the Weyl semimetal phase is robust in NbAs, and applying pressure may not be a good way to obtain a topological superconductor from Weyl semimetal NbAs.
In this paper, a novel fifth-degree cubature Kalman filter, which approaches the lower bound on the number of cubature points, is proposed to reduce the computational complexity while maintaining the ...fifth-degree filtering accuracy. The Gaussian weighted integral of a nonlinear function is approximated using a numerical cubature rule, whose number of cubature points needed is only one more than the theoretical lower bound, and the filter is deduced under the Bayesian filtering framework by this rule. Furthermore, a square-root version of the proposed fifth-degree cubature Kalman filter is given, and it acquires higher computational efficiency and ensures the numerical stability of the filter. Three numerical simulations are taken, and the results show that the proposed filters maintain the fifth-degree filtering accuracy, while needing the least amount of computation and achieving the best real-time performance.
Abstract
We investigate the evolution of superconductivity and structure with pressure for the new superconductor FeS (
T
c
≈ 4.5 K), a sulfide counterpart of FeSe. A rapid suppression of
T
c
and ...vanishing of superconductivity at 4.0 GPa are observed, followed by a second superconducting dome from 5.0 to 22.3 GPa with a 30% enhancement in maximum
T
c
. An onsite tetragonal to hexagonal phase transition occurs around 7.0 GPa, followed by a broad pressure range of phase coexistence. The residual deformed tetragonal phase is considered as the source of second superconducting dome. The observation of two superconducting domes in iron-based superconductors poses great challenges for understanding their pairing mechanism.
In the title compound, C(20)H(17)Cl(2)F(2)N(3)O(2), the triazole ring makes dihedral angles of 28.0 (3) and 72.5 (2)° with the 2,4-dichloro-pheny and 2,4-difluoro-phenyl rings, respectively, and the ...mol-ecule adopts a Z-conformation about the C=C double bond. In the crystal, C-H⋯O and C-H⋯N hydrogen bonds link the mol-ecules.
In the title compound, C(23)H(25)ClF(2)N(3)O(2) (+)·NO(3) (-), the triazole ring makes dihedral angles of 60.9 (4) and 25.0 (3)° with the 6-chloro-phenyl and 2,4-difluoro-phenyl rings, respectively. ...The mol-ecule adopts a Z configuration about the C=C double bond. In the crystal, the cations and anions are linked by N-H⋯O hydrogen bonds and weak C-H⋯O inter-actions.
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