To achieve high-resolution selection and separation of mono-isotopic beams through projectile fragmentation or in-flight fission and to efficiently transport the secondary beams to the Rare-RI Ring ...(R3) for mass determination, the BigRIPS separator combined with the High-resolution Achromatic (HA) beam-line of SHARAQ is served as a two-stage separator. The first stage from F0 to F2 is used for separation of the nuclei of interest through a B
ρ
-ΔE-B
ρ
method and the second stage from F3 to S0 is employed for identification of the beam with a B
ρ
-ΔE-TOF method. With this scheme, all the secondary ions can be well separated on an event by event basis and be utilized for mass measurements by two complementary methods: the conventional magnetic-rigidity-time-of-flight (B
ρ
-TOF) method employing the BigRIPS-HA beam-line to measure exotic nuclides within 1
μ
s and the newly established isochronous mass spectrometry (IMS) approach via the R3 with a measurement duration less than 1 ms. This report presents the technique advances of the BigRIPS-HA-SHARAQ-R3 as a separator/spectrometer and give a brief description of its application to the complementary TOF methods for mass measurements at RIBF.
A 43-year-old man presented with gastrointestinal bleeding. A tumor with central ulceration was observed in the jejunum, with the use of a new enteroscopy system called "double-balloon enteroscopy". ...Bleeding after biopsy sampling of the tumor was controlled endoscopically by using electrocoagulation. Histological findings of the biopsy specimens were consistent with gastrointestinal stromal tumor, and this was surgically resected. Double-balloon enteroscopy was useful for the diagnosis as well as the control of bleeding in this patient.
Because some of the autoreactive T‐cell clones specific for human PDC‐E2 cross‐react to mimicry peptides having an EIExDK motif derived from nuclear antigens such as human gp210 and sp100, we studied ...the clinical significance of antinuclear antibodies (ANA) in primary biliary cirrhosis (PBC) patients registered to the National Hospital Organization Study Group for Liver Disease in Japan (NHOSLJ). We found that there are two different types of progression in PBC; one is a hepatic failure‐type progression which is represented by positive anti‐gp210 antibodies and the other is a portalhypertension‐type progression which is represented by positive anticentromere antibodies. We discuss the predictive role of these ANA in the long‐term outcome of PBC and the mechanisms by which two different PBC progression types occur based on molecular mimicry and aberrant expression of nuclear antigens.
The Japan Society for Occupational Health (JSOH) recommends the Occupational Exposure Limits (OELs) as reference values for preventing adverse health effects on workers caused by occupational ...exposure to chemical substances, continuous or intermittent noise, impulsive or impact noise, heat stress, cold stress, whole-body vibration, hand-arm vibration and time-varying electric, magnetic and electromagnetic fields and ultraviolet and ionizing radiation. Characteristics of OELs and Instructions for Users 1. OELs should be applied by individuals well-trained and experienced in occupational health. 2. OELs cannot be applied in cases where exposure duration or work intensity exceeds the prerequisite conditions for setting an OEL. 3. OELs are set based on various information obtained from experiences in industries and experiments on humans and animals. However, the quantity and quality of information used in setting OELs is not always the same. 4. Types of health effects considered in setting OELs depend on the substances involved ; an explicit health impairment provides the basis for OELs in certain substances, while health effects such as discomfort, irritation or CNS suppressive effects afford the basis in others.
We previously described a color-coded imaging model that can quantify the length of nascent blood vessels using Gelfoam registered implanted in nestin-driven green fluorescent protein (ND-GFP) nude ...mice. In ND-GFP mice, nascent blood vessels are labeled with GFP. We report here that osteosarcoma cells promote angiogenesis in the Gelfoam registered angiogenesis assay in ND-GFP mice. Gelfoam registered was initially transplanted subcutaneously in the flank of transgenic ND-GFP nude mice. Seven days after transplantation of Gelfoam registered , skin flaps were made and human 143B osteosarcoma cells expressing green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in cytoplasm were injected into the transplanted Gelfoam registered . The control-group mice had only implanted Gelfoam registered . Skin flaps were made at days 14, 21, and 28 after transplantation of the Gelfoam registered to allow imaging of vascularization in the Gelfoam registered using a variable-magnification small animal imaging system and confocal fluorescence microscopy. ND-GFP expressing nascent blood vessels penetrated and spread into the Gelfoam registered in a time-dependent manner in both control and osteosarcoma-implanted mice. ND-GFP expressing blood vessels in the Gelfoam registered of the osteosarcoma-implanted mice were associated with the cancer cells and larger and longer than in the Gelfoam registered -only implanted mice (P<0.01). The results presented in this report demonstrate strong angiogenesis induction by osteosarcoma cells and suggest this process is a potential therapeutic target for this disease. J. Cell. Biochem. 115: 1490-1494, 2014. copyright 2014 Wiley Periodicals, Inc.
The integrin family of proteins has been shown to be involved in the malignant behavior of cells. We report here development of a color-coded imaging model that can visualize the interaction between ...αv integrin linked to green fluorescent protein (GFP) in osteosarcoma cells and blood vessels in Gelfoam® vascularized after implantation in red fluorescent protein (RFP) transgenic nude mice. Human 143B osteosarcoma cells expressing αv integrin-GFP were generated by transfection with an αv integrin-GFP vector. Gelfoam® (5×5 mm) was transplanted subcutaneously in transgenic RFP nude mice. The implanted Gelfoam® became highly vascularized with RFP vessels within 14 days. Skin flaps were made at days 7, 14, 21, 28 after transplantation of Gelfoam® for observing vascularization of the Gelfoam® using fluorescence imaging. Gelfoam® is a useful tool to observe angiogenesis in vivo. 143B cells (5 × 10(5)) expressing αv integrin-GFP were injected into the Gelfoam® seven days after transplantation of Gelfoam®. Seven days after cancer-cell injection, cancer cells and blood vessels were observed in the Gelfoam® by color-coded confocal microscopy via the skin flap. The 143B cells expressing αv integrin-GFP proliferated into the Gelfoam®, which contained RFP-expressing blood vessels. Strong expression of αv integrin-GFP in 143B cells was observed near RFP vessels in the Gelfoam®. The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells.
Breast reconstruction is performed to recover the lost breast shape after mastectomy, but the quantitative evaluation for breast shape has not been established.We have developed a system to acquire ...three dimensional information of the breast shape before mastectomy and breast reconstruction and to compute the local volume difference. We have reported on the function implementation and evaluation of projection mapping derived shape difference information to the skin surface of the patient in the previous report.Therefore, this study is to shorten the time from three dimensional measurement during intraoperative to volume difference calculation, projection mapping, and to evaluate operability in trial experiments in the operating room.The mannequin simulating the patient was placed in a supine position on a 45-degree operation table, and the projection position error by the conventional method was compared with the operation time.
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