► l-Cysteine stimulated proliferation and differentiation of NSCs. ► l-Cysteine-induced proliferation was associated with phosphorylation of ERK1/2. ► l-Cysteine-induced differentiation was due to ...the differentiation-related genes. ► AOAA or CBS siRNA attenuated the effects of l-cysteine on proliferation and differentiation of NSCs.
Growing evidence has suggested that hydrogen sulfide (H2S) acts as a novel neuro-modulator and neuroprotective agent; however, it remains to be investigated whether H2S has a direct effect on neural stem cells (NSCs). We report here that NSCs expressed cystathionine β synthase (CBS) and addition of exogenous H2S donor, l-cysteine, stimulated proliferation and increased the differentiation potential of NSCs to neurons and astroglia. Moreover, pre-treatment with aminooxyacetic acid, the inhibitor of CBS or knockdown of CBS in using siRNA, significantly attenuated the effects of l-cysteine on elevated H2S levels and the cell proliferation; it also effectively suppressed l-cysteine-induced neurogenesis and astrocytogenesis. Further analysis revealed that l-cysteine-induced proliferation was associated with phosphorylation of extracellular signal-regulated kinases 1/2 and differentiation with altered expression of differentiation-related genes. Taken together, the present data suggest that l-cysteine can enhance proliferation and differentiation of NSCs via the CBS/H2S pathway, which may serve as a useful inference for elucidating its role in regulating the fate of NSCs in physiological and pathological settings.
We report in situ observations of an electron jet generated by secondary reconnection within the outflow region of primary reconnection in the terrestrial magnetotail by the Magnetospheric Multiscale ...(MMS) mission. The MMS spacecraft first passed through the primary X-line and then crossed the electron jet in the outflow of primary reconnection. There are a series of small-scale flux ropes in the secondary reconnection region. Decoupling from the magnetic field for both ions and electrons, an intense out-of-plane current, unambiguous Hall currents, and a Hall electromagnetic field appear in the electron jet. Strong electron dissipation ( ), a nonzero electric field in the electron frame ( ), and electron crescent-like shaped distributions are detected in the center of the electron jet, implying that MMS spacecraft were likely passing through the electron diffusion region. The significant electron dissipation indicates that the electrons can be accelerated in the electron jet and the electron jet may be another important electron acceleration channel along with the electron diffusion region.
Kinetic‐size magnetic holes (KSMHs) in the turbulent magnetosheath are statistically investigated using high time resolution data from the Magnetospheric Multiscale mission. The KSMHs with short ...duration (i.e., <0.5 s) have their cross section smaller than the ion gyroradius. Superposed epoch analysis of all events reveals that an increase in the electron density and total temperature significantly increases (resp. decrease) the electron perpendicular (resp. parallel) temperature and an electron vortex inside KSMHs. Electron fluxes at ~90° pitch angles with selective energies increase in the KSMHs are trapped inside KSMHs and form the electron vortex due to their collective motion. All these features are consistent with the electron vortex magnetic holes obtained in 2‐D and 3‐D particle‐in‐cell simulations, indicating that the observed KSMHs seem to be best explained as electron vortex magnetic holes. It is furthermore shown that KSMHs are likely to heat and accelerate the electrons.
Key Points
Kinetic‐size magnetic holes are statistical investigated by MMS
Observed kinetic‐size magnetic holes seem to be best explained as electron vortex magnetic holes
Kinetic‐size magnetic holes are likely to heat and accelerate the electrons
Plain Language Summary
A nonlinear energy cascade in magnetized turbulent plasmas leads to the formation of different coherent structures which are thought to play an important role in dissipating energy and transporting particles. This study statistically investigate one new type of coherent structure, named electron vortex magnetic hole, used by Magnetospheric Multiscale data. It reveals the common features of this structure, including an increase in the electron density and total temperature, significantly increase (resp. decrease) the electron perpendicular (resp. parallel) temperature and an electron vortex inside these holes. The increase of electron temperature inside the holes indicates that these holes are likely to heat and accelerate the electrons. This gives new clue for energy dissipation in turbulent plasmas.
Turbulence is ubiquitous in space and astrophysical plasmas, such as the solar wind, planetary magnetospheres, and the interstellar medium. It plays a key role in converting electric and magnetic ...energies into kinetic energy of the plasma particles. Here, the properties of MHD and kinetic-scale magnetic fluctuations in the Mercury environment are investigated using data collected by the MESSENGER spacecraft from 2011 March 23 to 2015 April 28. It is found that spectral indices at MHD scales vary from ∼−5/3 in the near-Planet solar wind (possibly the foreshock) to ∼−1.3 within the magnetosheath close to bow shock. The spectra steepen further in the magnetosheath close to magnetopause, and reach ∼−2.2 within the magnetosphere. Only 15% of events were found to have the Kolmogorov scaling ∼−5/3 in the magnetosheath. The high variability of the spectral indices implies that the scaling of turbulent fluctuations in the magnetosheath is not universal, and it emphasizes the role of the bow shock on the turbulence dynamics, at least at the largest scales. Analysis of the magnetic compressibility shows that only ∼30% of events with Kolmogorov inertial range in the magnetosheath are dominated by (shear) Alfvénic fluctuations, which contrasts with well-known features of solar wind turbulence. At kinetic scales, the steepest spectra (slopes ∼−2.8) occur in the solar wind, before flattening to ∼−2 near the bow shock, then steepening again to ∼−2.8 in the magnetosheath. The spectral indices at kinetic scales are close to the ones at large scales in the magnetosphere, which may be caused by the presence of heavy ions in the latter. The statistical results are compared with previous observations reported in other planetary plasma environments.
Because of the lack of long-term pulsed emission in quiescence and the strong timing noise, it is impossible to directly measure the braking index n of a magnetar. Based on the estimated ages of ...their potentially associated supernova remnants (SNRs), we estimate the values of the mean braking indices of eight magnetars with SNRs, and find that they cluster in the range of 1–42. Five magnetars have smaller mean braking indices of 1 < n < 3, and we interpret them within a combination of magneto-dipole radiation and wind-aided braking. The larger mean braking indices of n > 3 for the other three magnetars are attributed to the decay of external braking torque, which might be caused by magnetic field decay. We estimate the possible wind luminosities for the magnetars with 1 < n < 3, and the dipolar magnetic field decay rates for the magnetars with n > 3, within the updated magneto-thermal evolution models. Although the constrained range of the magnetars’ braking indices is tentative, as a result of the uncertainties in the SNR ages due to distance uncertainties and the unknown conditions of the expanding shells, our method provides an effective way to constrain the magnetars’ braking indices if the measurements of the SNR ages are reliable, which can be improved by future observations.
Previous mass screening studies have shown that IgA antibodies against Epstein–Barr Virus (EBV) can facilitate early detection of nasopharyngeal carcinoma (NPC), but the impact of EBV-antibody ...screening for NPC-specific mortality remains unknown.
A prospective, cluster randomized, controlled trial for NPC screening (PRO-NPC-001) was conducted in 3 selected towns of Zhongshan City and 13 selected towns of Sihui City in southern China beginning in 2008. Serum samples of the screening group were tested for two previously selected anti-EBV antibodies. Subjects with serological medium risk were subsequently retested annually for 3 years, and those with serological high risk were referred to otorhinolaryngologists for diagnostic check-up. An interim analysis was carried out to evaluate the primary end points of the NPC-specific mortality and the early diagnostic rate, and the secondary end point of the NPC incidence, through linkage with the database of Zhongshan City.
Among 70 296 total subjects, 29 413 screened participants (41.8% of the total subjects) in the screening group and 50 636 in the control group, 153 (43.3 per 100 000 person-year), 62 (55.3 per 100 000 person-year) and 99 (33.1 per 100 000 person-year) NPC cases were identified. The early diagnostic rates of NPC were significantly higher in the participants (79.0%, P < 0.0001) and the screening group (45.9%, P < 0.0001) compared with the control group (20.6%). Although no differences were found between NPC-specific mortality of the screening group and the control group relative risk (RR)= 0.82, 95% confidence interval (CI) 0.37–1.79, lower NPC-specific mortality was noticed among participants from the screening group versus the control group (RR = 0.22, 95% CI 0.09–0.49).
IgA antibodies against EBV can identify high-risk population and was effective in screening for early asymptomatic NPC. Although the mortality reduction was not significant in the primary end point, we noted encouraging evidence of a mortality reduction in screening participants in this interim analysis.
NCT00941538.
We previously demonstrated that pretreatment quantitative anti–hepatitis B core protein (qAnti-HBc) levels can predict the treatment response for both interferon and nucleoside analogue therapy, but ...the characteristics of qAnti-HBc during chronic hepatitis B virus (HBV) infection remain poorly understood. To understand this issue, the qAnti-HBc levels were evaluated in individuals with past HBV infection, occult HBV infection and chronic HBV infection in the immune tolerance phase, immune clearance phase, low-replicative phase and hepatitis B e antigen (HBeAg)-negative hepatitis phase. Individuals with hepatitis B surface antigen (n = 598, 3.74 ± 0.90 log10 IU/mL) had significantly higher (p < 0.001, approximately 1000-fold) serum qAnti-HBc levels than those who had occult HBV, and serum qAnti-HBc levels were significantly higher in the occult HBV group than in the past HBV infection group (p < 0.001). qAnti-HBc levels were positively correlated with alanine aminotransferase levels (R = 0.663, p < 0.001), and subjects with an abnormal alanine aminotransferase level had a higher qAnti-HBc level (p < 0.001). Serum qAnti-HBc level varied in different phases of HBV infection, as determined by host immune status. Serum qAnti-HBc level is strongly associated with hepatitis activity in subjects with chronic HBV infection.
We present the first experimental evidence supported by simulations of kinetic effects launched in the interpenetration layer between the laser-driven hohlraum plasma bubbles and the corona plasma of ...the compressed pellet at the Shenguang-III prototype laser facility. Solid plastic capsules were coated with carbon-deuterium layers; as the implosion neutron yield is quenched, DD fusion yield from the corona plasma provides a direct measure of the kinetic effects inside the hohlraum. An anomalous large energy spread of the DD neutron signal (∼282 keV) and anomalous scaling of the neutron yield with the thickness of the carbon-deuterium layers cannot be explained by the hydrodynamic mechanisms. Instead, these results can be attributed to kinetic shocks that arise in the hohlraum-wall-ablator interpenetration region, which result in efficient acceleration of the deuterons (∼28.8 J, 0.45% of the total input laser energy). These studies provide novel insight into the interactions and dynamics of a vacuum hohlraum and near-vacuum hohlraum.
Hydrogels are promising materials in the applications of wound adhesives, wearable electronics, tissue engineering, implantable electronics, etc. The properties of a hydrogel rely strongly on its ...composition. However, the optimization of hydrogel properties has been a big challenge as increasing numbers of components are added to enhance and synergize its mechanical, biomedical, electrical, and self‐healable properties. Here in this work, it is shown that high‐throughput screening can efficiently and systematically explore the effects of multiple components (at least eight) on the properties of polysulfobetaine hydrogels, as well as provide a useful database for diverse applications. The optimized polysulfobetaine hydrogels that exhibit outstanding self‐healing and mechanical properties, have been obtained by high‐throughput screening. By compositing with poly(3,4‐ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), intrinsically self‐healable and stretchable conductors are achieved. It is further demonstrated that a polysulfobetaine hydrogel‐based electronic skin, which exhibits exceptionally fast self‐healing capability of the whole device at ambient conditions. This work successfully extends high‐throughput synthetic methodology to the field of hydrogel electronics, as well as demonstrates new directions of healable flexible electronic devices in terms of material development and device design.
A high‐throughput screening method, known as material genome engineering, has been employed to efficiently screen eight ingredients for the optimization of intrinsically stretchable and self‐healable polysulfobetaine hydrogels. The optimized hydrogels are successfully applied as electrodes and dielectric layer in an electronic skin, which displays impressively fast self‐healing and pressure sensing properties.
Large strain plasticity is phenomenologically defined as the ability of a material to exhibit an exceptionally large deformation rate during mechanical deformation. It is a property that is well ...established for metals and alloys but is rarely observed for ceramic materials especially at low temperature (∼300 K). With the reduction in dimensionality, however, unusual mechanical properties are shown by ceramic nanomaterials. In this Letter, we demonstrated unusually large strain plasticity of ceramic SiC nanowires (NWs) at temperatures close to room temperature that was directly observed in situ by a novel high-resolution transmission electron microscopy technique. The continuous plasticity of the SiC NWs is accompanied by a process of increased dislocation density at an early stage, followed by an obvious lattice distortion, and finally reaches an entire structure amorphization at the most strained region of the NW. These unusual phenomena for the SiC NWs are fundamentally important for understanding the nanoscale fracture and strain-induced band structure variation for high-temperature semiconductors. Our result may also provide useful information for further studying of nanoscale elastic−plastic and brittle−ductile transitions of ceramic materials with superplasticity.
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