Soil is characterized by high spatiotemporal variability due to the combined influence of internal and external factors. The most efficient approach for addressing spatial variability is the use of ...management zones (MZs). Common approaches for delineating MZs include K-means and fuzzy C-means cluster analysis algorithms. However, these clustering methods have been used to delineate MZs independent of the spatial dependence of soil variables. Thus, the accuracy of the clustering results has been limited. In this study, six soil variables (soil pH, total nitrogen, organic matter, available phosphorus, available potassium, and soil apparent electrical conductivity) were used to characterize the spatial variability within a representative village in Suining County, Jiangsu Province, China. Two variable reduction techniques (PCA, multivariate spatial analysis based on Moran’s index; MULTISPATI-PCA) and three different clustering algorithms (fuzzy C-means clustering, iterative self-organizing data analysis techniques algorithm, and Gaussian mixture model; GMM) were used to optimize the MZ delineation. Different clustering model composites were evaluated using yield data collected after the wheat harvest in 2020. The results indicated that the variable reduction technologies in conjunction with clustering algorithms provided better performance in MZ delineation, with average silhouette coefficient (ASC) and variance reduction (VR) of 0.48–0.57, and 13.35–23.13%, respectively. Moreover, the MULTISPATI-PCA approach was more conducive to identifying variables requiring MZ delineation than traditional PCA methods. Combining MULTISPATI-PCA and the GMM algorithm yielded the greatest VR and ASC values in this study. These results can guide the optimization of MZ delineation in intensive agricultural systems, thus enabling more precise nutrient management.
Cerebral neoplasms like gliomas may cause intracranial pressure increasing, neural tract deviation, infiltration, or destruction in peritumoral areas, leading to neuro-functional deficits. Novel ...tracking technology, such as DTI, can objectively reveal and visualize three-dimensional white matter trajectories; in combination with intraoperative navigation, it can help achieve maximum resection whilst minimizing neurological deficit. Since the reconstruction of DTI raw data largely relies on the technical engineering and anatomical experience of the operator; it is time-consuming and prone to operator-induced bias. Here, we develop new user-friendly software to automatically segment and reconstruct functionally active areas to facilitate precise surgery. In this pilot trial, we used an in-house developed software (DiffusionGo) specially designed for neurosurgeons, which integrated a reliable diffusion-weighted image (DWI) preprocessing pipeline that embedded several functionalities from software packages of FSL, MRtrix3, and ANTs. The preprocessing pipeline is as follows: 1. DWI denoising, 2. Gibbs-ringing removing, 3. Susceptibility distortion correction (process if opposite polarity data were acquired), 4. Eddy current and motion correction, and 5. Bias correction. Then, this fully automatic multiple assigned criteria algorithms for fiber tracking were used to achieve easy modeling and assist precision surgery. We demonstrated the application with three language-related cases in three different centers, including a left frontal, a left temporal, and a left frontal-temporal glioma, to achieve a favorable surgical outcome with language function preservation or recovery. The DTI tracking result using DiffusionGo showed robust consistency with direct cortical stimulation (DCS) finding. We believe that this fully automatic processing pipeline provides the neurosurgeon with a solution that may reduce time costs and operating errors and improve care quality and surgical procedure quality across different neurosurgical centers.
For precise delineation of glioma extent, amino acid PET is superior to conventional MR imaging. Since metabolic MR sequences such as chemical exchange saturation transfer (CEST) imaging and MR ...spectroscopy (MRS) were developed, we aimed to evaluate the diagnostic accuracy of combined CEST and MRS to predict glioma infiltration. Eighteen glioma patients of different tumor grades were enrolled in this study; 18F-fluoroethyltyrosine (FET)-PET, amide proton transfer CEST at 7 Tesla(T), MRS and conventional MR at 3T were conducted preoperatively. Multi modalities and their association were evaluated using Pearson correlation analysis patient-wise and voxel-wise. Both CEST (R = 0.736, p < 0.001) and MRS (R = 0.495, p = 0.037) correlated with FET-PET, while the correlation between CEST and MRS was weaker. In subgroup analysis, APT values were significantly higher in high grade glioma (3.923 ± 1.239) and IDH wildtype group (3.932 ± 1.264) than low grade glioma (3.317 ± 0.868, p < 0.001) or IDH mutant group (3.358 ± 0.847, p < 0.001). Using high FET uptake as the standard, the CEST/MRS combination (AUC, 95% CI: 0.910, 0.907−0.913) predicted tumor infiltration better than CEST (0.812, 0.808−0.815) or MRS (0.888, 0.885−0.891) alone, consistent with contrast-enhancing and T2-hyperintense areas. Probability maps of tumor presence constructed from the CEST/MRS combination were preliminarily verified by multi-region biopsies. The combination of 7T CEST/MRS might serve as a promising non-radioactive alternative to delineate glioma infiltration, thus reshaping the guidance for tumor resection and irradiation.
•Any decellularization impairs tracheal cartilage, trachealis, and connective tissue.•Treatment causes loss of Coll-II and GAGs, leading to collapse of cartilage rings.•Coll-III is lost with any ...treatment whereas Col-I becomes disorganized and degrades.•Trachealis muscle becomes more compliant with loss of contractile SMCs.•Axial mechanics are governed by collagen structure and longitudinal elastin fibers.
Tracheal replacement using tissue engineering technologies offers great potential to improve previously intractable clinical interventions, and interest in this area has increased in recent years. Many engineered airway constructs currently rely on decellularized native tracheas to serve as the scaffold for tissue repair. Yet, mechanical failure leading to airway narrowing and collapse remains a major cause of morbidity and mortality following clinical implantation of decellularized tracheal grafts. To understand better the factors contributing to mechanical failure in vivo, we characterized the histo-mechanical properties of tracheas following two different decellularization protocols, including one that has been used clinically. All decellularized tracheas deviated from native mechanical behavior, which may provide insights into observed in vivo graft failures. We further analyzed protein content by western blot and analyzed microstructure by histological staining and found that the specific method of decellularization resulted in significant differences in the depletion of proteoglycans and degradation of collagens I, II, III, and elastin. Taken together, this work demonstrates that the heterogeneous architecture and mechanical behavior of the trachea is severely compromised by decellularization. Such structural deterioration may contribute to graft failure clinically and limit the potential of decellularized native tracheas as viable long-term orthotopic airway replacements.
Display omitted
Introduction
Chronic cerebral hypoperfusion has been considered the etiology for sporadic Alzheimer's disease (AD). However, no valid clinical evidence exists due to the similar risk factors between ...cerebrovascular disease and AD.
Methods
We used moyamoya disease (MMD) as a model of chronic hypoperfusion and cognitive impairment, without other etiology interference.
Results
Based on the previous reports and preliminary findings, we hypothesized that chronic cerebral hypoperfusion could be an independent upstream crucial variable, resulting in AD, and induce pathological hallmarks such as amyloid beta peptide and hyperphosphorylated tau accumulation.
Discussion
Timely intervention with revascularisation would help reverse the brain damage with AD hallmarks and lead to cognitive improvement.
Engineered whole lungs may one day expand therapeutic options for patients with end-stage lung disease. However, the feasibility of ex vivo lung regeneration remains limited by the inability to ...recapitulate mature, functional alveolar epithelium. Here, we modulate multimodal components of the alveolar epithelial type 2 cell (AEC2) niche in decellularized lung scaffolds in order to guide AEC2 behavior for epithelial regeneration. First, endothelial cells coordinate with fibroblasts, in the presence of soluble growth and maturation factors, to promote alveolar scaffold population with surfactant-secreting AEC2s. Subsequent withdrawal of Wnt and FGF agonism synergizes with tidal-magnitude mechanical strain to induce the differentiation of AEC2s to squamous type 1 AECs (AEC1s) in cultured alveoli, in situ. These results outline a rational strategy to engineer an epithelium of AEC2s and AEC1s contained within epithelial-mesenchymal-endothelial alveolar-like units, and highlight the critical interplay amongst cellular, biochemical, and mechanical niche cues within the reconstituting alveolus.
The development of an
system for the study of lung vascular disease is critical to understanding human pathologies. Conventional culture systems fail to fully recapitulate native microenvironmental ...conditions and are typically limited in their ability to represent human pathophysiology for the study of disease and drug mechanisms. Whole organ decellularization provides a means to developing a construct that recapitulates structural, mechanical, and biological features of a complete vascular structure. Here, we developed a culture protocol to improve endothelial cell coverage in whole lung scaffolds and used single-cell RNA-sequencing analysis to explore the impact of decellularized whole lung scaffolds on endothelial phenotypes and functions in a biomimetic bioreactor system. Intriguingly, we found that the phenotype and functional signals of primary pulmonary microvascular revert back-at least partially-toward native lung endothelium. Additionally, human induced pluripotent stem cell-derived endothelium cultured in decellularized lung systems start to gain various native human endothelial phenotypes. Vascular barrier function was partially restored, while small capillaries remained patent in endothelial cell-repopulated lungs. To evaluate the ability of the engineered endothelium to modulate permeability in response to exogenous stimuli, lipopolysaccharide (LPS) was introduced into repopulated lungs to simulate acute lung injury. After LPS treatment, proinflammatory signals were significantly increased and the vascular barrier was impaired. Taken together, these results demonstrate a novel platform that recapitulates some pulmonary microvascular functions and phenotypes at a whole organ level. This development may help pave the way for using the whole organ engineering approach to model vascular diseases.
Numerous studies have linked visceral adipose tissue (VAT) to gastrointestinal diseases. However, it remains unclear whether these associations reflect causal relationships.
We used a two-sample ...Mendelian randomization (MR) approach to elucidate the causal effect of VAT on nine non-tumour gastrointestinal diseases. The inverse-variance weighted method was used to perform the MR analyses. Complementary and multivariable MR analyses were performed to confirm the results.
Genetically predicted higher VAT was associated with an increased risk of gastro-oesophageal reflux disease (GORD) (odds ratio OR, 1.21; 95% confidence interval CI, 1.09-1.34; P = 3.06 × 10
), duodenal ulcer (DU) (OR, 1.40; 95% CI, 1.10-1.77; P = 0.005), cholelithiasis (OR, 1.75; 95% CI, 1.53-2.00; P = 1.14 × 10
), and non-alcoholic fatty liver disease (NAFLD) (OR, 2.68; 95% CI, 1.87-3.82; P = 6.26 × 10
). There were suggestive associations between VAT and gastric ulcer (GU) (OR, 1.22; 95% CI, 1.01-1.48; P = 0.035) and acute pancreatitis (AP) (OR, 1.26; 95% CI, 1.05-1.52; P = 0.013). However, there was little evidence to support the associations between VAT and inflammatory bowel disease, irritable bowel syndrome, or chronic pancreatitis. The associations with GORD, GU, and NAFLD remained in the multivariable MR analyses with adjustment for body mass index (BMI).
This study provided evidence in support of causal associations between VAT and GORD, GU, DU, cholelithiasis, AP, and NAFLD. Moreover, the associations between GORD, GU, and NAFLD were independent of the effect of BMI.
Due to the frequent deployment of underwater acoustic sensor networks (UASNs) in harsh and unattended environments, they are particularly vulnerable to sensor node failures, energy exhaustion, and ...malicious attacks. These threats can lead to cascading failures, resulting in the collapse of the entire sensor network. Therefore, appropriate cascading failure models are urgently needed to analyze the network failure process and help design invulnerability networks. In this article, we first introduce the nodes' dynamic distribution and underwater routing protocols to establish a complex network model of UASNs, to represent the characteristics and behaviors of network sensor nodes, dynamic topologies, and routing protocols. Furthermore, we propose the cascading failure model designed specifically for based-depth-routing (BDR) and based-energy-routing (BER) protocol UASNs. In this cascading failure model, we analyze the traffic characteristics of the two types of routing protocol UASNs and establish the corresponding load redistribution scheme. Through extensive simulations and comprehensive multiangle analysis, we have obtained some meaningful results: 1) in some special deployment situations, such as the node deployment distance is 0.9 times the node communication radius, the UASN nodes' dynamics can actually increase the invulnerability of the network; 2) the routing protocols of UASNs can resist small-scale attacks by self-organizing the distribution of network load; and 3) the network node deployed distance is a key factor affecting network invulnerability. These conclusions offer valuable assistance in the design of UASNs that possess enhanced invulnerability.
Emerging NUMA/CXL-based tiered memory systems with heterogeneous memory devices such as DRAM and NVMM deliver ultrafast speed, large capacity, and data persistence all at once, offering great promise ...to high-performance in-memory key-value stores. To fully unleash the performance potential of such memory systems, this paper presents BonsaiKV, a key-value store that makes the best use of different components in a tiered memory system. The core of BonsaiKV is a tri-layer hierarchical storage architecture that separates data indexing, persistence, and scalability from each other and realizes each of them within a specialized software-hardware layer. We design BonsaiKV with a set of novel techniques, including collaborative tiered indexing, NVMM congestion control mechanisms, fine-grained data striping, and NUMA-aware data management, to leverage hardware strengths and tackle device deficiencies. We compare BonsaiKV with state-of-the-art NVMM-optimized key-value stores and persistent index structures using a variety of YCSB workloads. Evaluation results demonstrate that BonsaiKV outperforms others by up to 7.69×, 19.59×, and 12.86× in read-, write- and scan-intensive scenarios, respectively.
PDF
