It is very well known in the planning publications that when creating spacing development to a region or sub-region, it can be able to make more than an alternative consisting with the strategic ...directions overtaken from the actual development of region and the situational and developmental objectives needed. However, the difficulty facing the situational planning is in selecting one of these alternatives to be the best in order to make a balanced situational re-structure, and achieving the economic, social and civil objectives. The developmental situation elements in the regions and governorates, including (Karbala) impose themselves as situational power which implies the process of re-structural arrangement where the situational development is considered the active method for such arrangement. Thus, the difficulty of making decision in selecting any of the developmental alternatives seems easier for the situational planner as he has the programming methods and operations researches. The scientific method is used for making decision to suggest the developmental alternative which can achieve the strategic comprehension of the development elements and ensures the exploitation better to achieve the decree objectives. The method of decisions making theory is considered one scientific method which can be used in this regard. They mechanism of the decisions theory implies making more than one alternative to arrange the strategies and objectives in a matrix through which can make more than a case of making decision towards selecting the suitable situational strategy to achieve the objectives under the case of making decision brunt determined by the interdependent aspect in making decision. This necessitates knowledge of the developmental reality of the studied region or province. Therefore, the applied study of the developmental reality in the Karbala (region) province showed that there are two strategies that can be adopted to bring about spatial development in that province: the balanced growth strategy in the first, rational aspect drawn for sectors for development and the decision to maximize returns, and in the second aspect (cumulative) and the third (participatory), as well as the application proved the possibility of adopting the strategy of manufacturing and manufacturing for export in order to achieve a special type of goals at the lowest costs, whether in the case of certainty or risk, and this leaves the room clear for the decision maker to adopt any of the two strategies.
Abstract
In this paper, a new method of selection variables is presented to select some essential variables from large datasets. The new model is a modified version of the Elastic Net model. The ...modified Elastic Net variable selection model has been summarized in an algorithm. It is applied for Leukemia dataset that has 3051 variables (genes) and 72 samples. In reality, working with this kind of dataset is not accessible due to its large size. The modified model is compared to some standard variable selection methods. Perfect classification is achieved by applying the modified Elastic Net model because it has the best performance. All the calculations that have been done for this paper are in R program by using some existing packages.
The increase in bacterial infections and the emergence of antibiotic resistance has led to the development of bio-derived and environmentally friendly nanomaterials to counteract bacterial activity, ...and silver nanoparticles AgNPs are a promising way to achieve this. The present work aimed to synthesize AgNPs from the blue-green algae Athrospira Platensis and evaluate their antibacterial efficacy against Enterococcus faecalis, Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa isolated from human urinary tract infections. The results were shown and confirmed by peak UV spectra at 450. Through SEM and DLS analyses, the shapes of the AgNPs were determined to be spherical and with sizes ranging from 15 to 100 nm. The zeta potential values of AgNPs were found to be −20 ± 3.2 mV indicating the absence of AgNPs aggregates. Synthetic AgNPs produced by A. platensis showed a strong antibacterial effect against bacteria isolated from human urinary tract infections. The diameters of the inhibitory zone for S. aureus E. faecalis P. mirabilis and P. aeruginosa 14 mm, 16 mm, 15 mm, and 12 mm, respectively. AgNPs showed the highest activity against E. faecalis strain, with the diameter of the inhibitory zone reaching 16 ± 0.2 mm at a concentration of 800 μg/mL.
These results will be a useful resource for studying the effects of man-made nanoparticles on the environmental safety and represent a promising step toward realizing AgNP-based antimicrobial drug production.
BACKGROUND: Multiple Sclerosis known as MS, this chronic inflammatory demyelinating condition affects the nervous system. It is a heterogenic and multifactorial disease. The goal of the current study ...was to investigate the relationship between MS patients’ IL18 gene expression and the vitamin D receptor gene polymorphism (FOK1rs2228570). OBJECTIVE: The aim of the study to investigate the association of vitamin D receptor (FOK1rs2228570) gene polymorphism and pro inflammatory cytokine (IL18) gene expression among multiple sclerosis Iraqi patients. Detection VDR polymorphism and determine whether this SNP is involved in susceptibility to multiple sclerosis and estimation IL18 gene expression and explore its relation with multiple sclerosis susceptibility. METHODS: Blood samples were taken from 75 MS patients in Iraq (30 men and 45 women), as well as from 75 volunteers who seemed to be in a favorable state of health and fell within the age range of 20 to 50 years. Tetra-ARMS Polymerase Chain Reaction (Tetra-ARMS PCR) was used to find polymorphisms in the vitamin D receptor (VDR) gene, and Real-time Polymerase Chain Reaction (RT-PCR) was used to measure IL18 gene expression. RESULTS: The findings from the analysis of VDR gene polymorphism in patients with MS indicated that the wild-type genotype T/T was present in 8 individuals, accounting for 10.6%, the heterogeneous genotype TC was 36 (48%), and the homogeneous genotype CC was 31 (41.3%), whilst T allele frequency was 52(34.6%) and C allele was 98(65.3%) with (P⩽ 0.01) significant difference and even as in control T/T genotype was 49(65.3%), TC genotype was 21(28%), CC genotype was 5(6.66%), T allele frequency was 119(79.3%) and C allele was 31(20.6%) with significant difference (P⩽ 0.001). While estimation of IL18 expression showed high elevation in patients’ group (2.59 ± 0.51 fold) by significance difference (P⩽ 0.5) when compared to control group (1.35 ± 0.14 fold). The relationship between IL18 gene expression with VDR variant in MS patients demonstrated a significant rise (2.9 ± 0.51 fold) at CC genotype patients in IL18 folding gene expression, followed by (4.6 ± 0.17 fold) in TC genotype patients and finally (1.4 ± 0.08 fold) in TT genotype patients with highly significant (P⩽ 0.01). CONCLUSION: The VDR(FOK1rs2228570) genotype was significantly correlated with IL18 expression in MS patients from Iraq.
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Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
In recent years, global pharmaceutical consumption has increased, resulting in the increased release into the environment and endangering the entire ecosystem. These pharmaceuticals have attracted ...considerable attention due to their persistence, toxicity, and the appearance of resistance genes and development antibiotic-resistance bacteria. Furthermore, conventional wastewater treatment plants are ineffective in treating antibiotic-contaminated wastewater.Thus, algae-based technologies are sustainable, low-cost, and friendly to the environment. In this context, immobilization appears to be of particular interest to many researchers as they develop new, efficient, greener strategies for the elimination of toxic and hazardous pollutants. provide a critical overview of algal immobilization-based technologies, and a biotechnological tool that restricts cell movement by confining it within a polymer matrix or attaching it to a rigid support is a promising ,and cost-effective alternative that does not necessitate the use of additional chemicals. This paper presents strategies for the systematic removal of pharmaceuticals based on algae immobilization techniques as an economical, effective, and feasible alternative technology for removing pharmaceuticals and environmental concerns from water bodies and discusses the benefits and drawbacks of these techniques
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•Porphyrin-based nanoarchitectures demonstrate significant advancements in sensing applications.•Integration of porphyrins into various nanostructures offers unique advantages for ...specific sensing scenarios.•Emphasis on synthesis, characterization, and applications in gas, biosensing, and environmental monitoring.•Review addresses challenges, future perspectives, and emerging trends in sensing technologies.•Synergy between porphyrin properties and nanotechnology shapes the future of advanced sensing technologies.
Recent years have seen a significant advancement in the development of porphyrin-based nanoarchitectures for sensing applications. Porphyrins, known for their remarkable electronic, optical, and catalytic properties, have been ingeniously integrated into various nanoarchitectures such as nanoparticles, nanocomposites, nanotubes, nanosheets, and other nanostructures, each offering unique advantages for specific sensing scenarios. This review article synthesizes the progress in this field, examining the synergy between porphyrin properties and nanotechnology to address the increasing demands in environmental monitoring, healthcare diagnostics, and industrial applications. Emphasis is placed on synthesis and fabrication techniques, characterization methods, and the burgeoning variety of applications in gas, biosensing, and environmental sensing. Porphyrin-based nanoarchitectures demonstrate enhanced sensing capabilities in various fields due to their intrinsic properties and strategic design. Overcoming scalability, cost, and integration challenges while embracing innovation holds the key to further advancing their utility in sensing applications.
Background. Increased FOXP3+ Treg levels in the TME were positively correlated with a worse prognosis in certain cancer patients. FOXP3 was significantly overexpressed in pediatric B-ALL patients, ...and this overexpression was associated with a worse prognosis and increased risk of disease relapse. It is unknown if children that have the condition would have different prenatal immune development. This study investigates the relationship between IL10, and immunological development in pediatric ALL. Methods. The 70 blood samples from children between the ages of 2 and 14 for both sexes were obtained from patients with Acute lymphoblastic leukemia. Furthermore, this study comprised 54 healthy controls. Included in this study, FOXP3 expression on leukemic blast cells were assessed using flow cytometry. And IL10 were assessed using ELISA test. Results. Increase of FOXP3 cells was noticed in children with ALL compared to healthy individuals with significant increase of FOXP3 (mean ± SD, 91.156 ± 12.255 vs. 14.88 ± 7.897pg/ml (p <0.05). In light of these findings, significant differences in the levels of FOXP3, which was higher in consolidation than induction stage of chemotherapy (mean ± SD, 91.24± 11.078vs. 86.35±5. 16.66) pg/ml (p <0.05). Additionally, it was found that the prevalence of lymphoblast that express FOXP3 was notably higher in the HR group in contrast to the SR group (mean ± SD, 95.52± 5.79 vs. 42.88± 31.38) pg/ml (p <0.05). Following these findings, significant differences in the levels of FOXP3 was higher in relapse than new diagnosis chemotherapy stage (mean ± SD, 95.66± 5.167 vs. 91.035±5. 13.177) pg/ml (p<0.05). On the other hand, children with ALL were also found to have significantly higher levels of IL10 in the current study when compared to healthy controls (mean ± SD, 0.146 ± 0.145 vs. 0.076 ± 0.10 pg/ml (P <0.05). Following these findings, significant differences in the levels of IL10 was higher in consolidation than induction chemotherapy stage (mean ± SD, 0.165± 0.084 vs.0 .0720±.018) pg/ml (p <0.05), the serum levels of IL-10 were evaluated in the two groups: new diagnosis and relapse cases. As in the description profile, serum levels are higher in the relapse group with a highly significant difference. For the parameter IL10 (P ≤0.05). As for he mean level, it was as follows: of IL10 (0.130) pg/ml and (0.216) pg/ml, with (P-value =0.0001) in new diagnosis and relapse receptivity. Conclusions. According to our findings, B-ALL patients have significant amounts of both FOXP3 and IL10. This pilot study provides a novel way to look into the process mediating the appearance of these markers in a greater number of B-ALL patients at various stages of their treatment.
Livedoid vasculopathy (LV) is a chronic, recurrent thrombotic vasculopathy characterized by painful ulcerations on the lower extremities, which heal slowly and leave atrophic white scars known as ...“atrophie blanche.” This report presents the case of a 31-year-old woman with a 4-year history of recurrent painful ulcerations on her legs and feet. A skin biopsy revealed findings consistent with LV, and an exhaustive laboratory workup ruled out secondary causes such as thrombophilia, malignancies, autoimmune diseases, and peripheral arterial disease. The patient showed remarkable improvement with a treatment regimen of pentoxifylline, nifedipine, and warfarin, resulting in complete ulcer resolution and sustained remission over 5 months. Our case highlights the importance of a comprehensive diagnostic approach and a multidisciplinary treatment strategy in managing primary LV to achieve remission and prevent recurrence of skin ulcerations.
Applications of microalgae in environmental studies have recently increased. Current uses of immobilized microalga Chlorella vulgaris include reducing pharmaceutical substances such as amoxicillin ...AMX and potassium dichromate K2Cr2O7 on freshwater clam Pseudodontopsis euphraticus as a biotic model. Recent research pointed out a change in biomarkers of oxidative stress in an evaluation of induced toxicity. Where clams were exposed to different concentrations100, 200, and 400 mg/L for 7 days and 20, 30, and 50 mg/L for 5 days of amoxicillin and potassium dichromate, respectively. The results showed that exposure to AMX and K2Cr2O7 led to a significant change in the activity of antioxidant enzymes, with significant increases (p<0.05) in reactive oxygen species (ROS) production. The highest ROS value was 51.05 μg/mg under concentrations of 50 mg/L of K2Cr2O7, and the highest recorded percentage of Superoxide Dismutase SOD, Catalase CAT, Malondialdehyde MDA, and Glutathione Reductase GSH, as: 33.40 U/m, 33.32KU/L, 23.22 μmol/l and 21.30µg/g respectively, in concentrations of 50 mg/L of K2Cr2O7 non-treated. It was observed in this study that potassium dichromate was more effective than amoxicillin in causing toxicity. According to the current study, immobilized C. vulgaris was instrumental in decreasing chemicals toxicity, by relieving oxidative stress on P. euphraticus clam, as it recorded a significant decrease p≤ 0.05 in ROS values and oxidizing enzymes such as Superoxide Dismutase SOD, Catalase CAT, Malondialdehyde MDA, as well as ascorbic acid. AA, total protein and GPX in treated samples.
BACKGROUND: Multiple sclerosis (MS) is a long-term condition characterized by chronic inflammation, damage to the myelin sheath, and progressive nerve cell degeneration. It is a heterogeneous and ...multifactorial disease. The aim of the present investigation was to analyze the connection between variations in the vitamin D receptor gene. (APAI rs7975232) and vitamin D serum levels among MS patients. METHODS: Blood samples were collected from 75 Iraqi patients with MS (33 male, 42 female), and 75 control group volunteers who appeared to be in good health with an age range of 20–50 years. Vitamin D receptor (VDR) gene polymorphism was detected by HRM RT-PCR and vitamin D serum levels were assessed by ELISA. RESULTS: Detection of VDR gene polymorphism in MS patients discovered that the wild genotype was C/C 15 (20%), the heterozygous genotype CA was 27(36%), and the homozygous genotype AA was 33(44%), whilst allele C occurrence was 57(38%) and allele A was 93(62%), compared per control genotype C/C was 40(53.3%), CA genotype was 20(26.6%), AA genotype was 15(20%), C allele frequency was 100(66.6%) and A allele was 50(33.3%) with highly significant difference (P≤0.001). Analysis of vitamin D serum levels showed much higher levels in the control group (43.40±0.85 pg/ml) than in the MS patients group (15.46±0.93 pg/ml; P≤0.001). Result of relationship between Vitamin D serum level with genotype of VDR among individuals with MS was found to be significant decrease (5.3±0.52) at AA genotype of MS patients, followed by (11.79±0.68) in CA genotype and finally (15.52±0.93) in CC genotype, all highly significant (P≤0.01). CONCLUSION: There was a notable correlation observed with VDR (APAI rs7975232) genotypes and Vitamin D serum level in MS Iraqi patients.
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