A
bstract
Using data samples with an integrated luminosity of 6
.
4 fb
−
1
collected by the BESIII detector operating at the BEPCII storage ring, the process of
e
+
e
−
→
γϕJ/ψ
is studied. The ...processes of
e
+
e
−
→
ϕχ
c
1
,
c
2
,
χ
c
1
,
c
2
→
γJ/ψ
are observed with a significance of more than 10
σ
. The
$$ \sqrt{s} $$
s
-dependent cross section of
e
+
e
−
→
ϕχ
c
1
,
c
2
is measured between 4.600 and 4.951 GeV, and evidence of a resonance structure is found for the first time in the
ϕχ
c
2
process. We also search for the processes of
e
+
e
−
→
γX
(4140),
γX
(4274) and
γX
(4500) via the
γϕJ/ψ
final state, but no obvious structures are found. The upper limits on the production cross section times the branching fraction for these processes at the 90% confidence level are reported.
By analyzing 2.93 fb−1 of e+e− annihilation data taken at the center-of-mass energy √s = 3.773 GeV with the BESIII detector, we determine the branching fractions of the inclusive decays D+ → ϕX and ...D0 → ϕX to be (1.135±0.034±0.031)% and (1.091±0.027±0.035)%, respectively, where X denotes any possible particle combination. The first uncertainties are statistical, and the second are systematic. We also determine the branching fractions of the decays D → ϕX and their charge conjugate modes D¯ → ϕX¯ separately for the first time, and no significant CP asymmetry is observed.
A
bstract
A partial wave analysis on the process
e
+
e
−
→
ωπ
+
π
−
is performed using 647 pb
−
1
of data sample collected by using the BESIII detector operating at the BEPCII storage ring at ...center-of-mass (c.m.) energies from 2.000 GeV to 3.080 GeV. The Born cross section of the
e
+
e
−
→
ωπ
+
π
−
process is measured, with precision improved by a factor of 3 compared to that of previous studies. A structure near 2.25 GeV is observed in the energy-dependent cross sections of
e
+
e
−
→
ωπ
+
π
−
and
ωπ
0
π
0
with a statistical significance of 7.6
σ
, and its determined mass and width are 2232 ± 19 ± 27 MeV
/c
2
and 93 ± 53 ± 20 MeV, respectively, where the first and second uncertainties are statistical and systematic, respectively. By analyzing the cross sections of subprocesses
e
+
e
−
→
ωf
0
(500),
ωf
0
(980),
ωf
0
(1370),
ωf
2
(1270), and
b
1
(1235)
π
, a structure, with mass M = 2200 ± 11 ± 17 MeV/
c
2
and width Γ = 74 ± 20 ± 24 MeV, is observed with a combined statistical significance of 7.9
σ
. The measured resonance parameters will help to reveal the nature of vector states around 2.25 GeV.
We present an amplitude analysis of the decay D 0 → K - π + π + π - based on a data sample of 2.93 fb −1 acquired by the BESIII detector at the ψ(3770) resonance. With a nearly background free ...sample of about 16000 events, we investigate the substructure of the decay and determine the relative fractions and the phases among the different intermediate processes. Our amplitude model includes the two-body decays D 0 → ¯K *0 ρ 0 , D 0 → K − a + 1 (1260) and D 0 → K − 1 (1270)π + , the three-body decays D 0 →¯K *0 π + π − and D 0 → K − π + ρ 0 , as well as the four-body nonresonant decay D 0 → K − π + π + π − . The dominant intermediate process is D 0 → K − a + 1 (1260), accounting for a fit fraction of 54.6%.
This study was performed to compare the effects of neck dissection procedures on the prognosis of patients with pathological N1 (pN1) oral squamous cell carcinoma (OSCC), analyse factors affecting ...the prognosis, and provide a neck management strategy for clinical N1 (cN1) oral cancer. The study patients were divided into two groups according to the neck dissection: a selective neck dissection (SND) group (n = 85) and a radical or modified radical neck dissection (RND/MRND) group (n = 22). There was no statistically significant difference in recurrence rates at local, regional, and distant sites between the SND and RND/MRND groups. The 5-year overall survival was 68.3% for SND and 65.2% for RND/MRND patients (P = 0.590), while the 5-year disease-specific survival was 70.4% for SND and 75.7% for RND/MRND patients (P = 0.715). Histological grade and postoperative radiotherapy were independent predictors of the outcome for SND patients. For histological grade II/III cases, 5-year overall survival (P = 0.004) and disease-specific survival (P = 0.002) outcomes differed significantly between patients treated with and without postoperative radiotherapy, with worse survival for patients not treated with radiotherapy. Therefore, SND appears appropriate for cN1 OSCC patients, and postoperative radiotherapy is recommended for those with histological grade II or III tumours.
Elucidating the cellular organization of the cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing and spatial transcriptomic ...analysis of 143 macaque cortical regions, we obtained a comprehensive atlas of 264 transcriptome-defined cortical cell types and mapped their spatial distribution across the entire cortex. We characterized the cortical layer and region preferences of glutamatergic, GABAergic, and non-neuronal cell types, as well as regional differences in cell-type composition and neighborhood complexity. Notably, we discovered a relationship between the regional distribution of various cell types and the region’s hierarchical level in the visual and somatosensory systems. Cross-species comparison of transcriptomic data from human, macaque, and mouse cortices further revealed primate-specific cell types that are enriched in layer 4, with their marker genes expressed in a region-dependent manner. Our data provide a cellular and molecular basis for understanding the evolution, development, aging, and pathogenesis of the primate brain.
Display omitted
•A comprehensive cell-type taxonomy is constructed for the entire macaque cortex•Stereo-seq reveals the global distribution of 264 cell types and their marker genes•Regional density and composition of cell types are coupled with cortical hierarchy•Cross-species analysis revealed primate-specific cell types enriched in layer 4
A spatially resolved single-cell transcriptome atlas of macaque cortex is generated that reveals the organization and evolution of primate cortical regions.
Background. Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by the SFTS virus (SFTSV) with an average fatality rate of 12%. The clinical factors for death ...in SFTS patients remain unclear. Methods. Clinical features and laboratory parameters were dynamically collected for 11 fatal and 48 non-fatal SFTS cases. Univariate logistic regression was used to evaluate the risk factors associated with death. Results. Dynamic tracking of laboratory parameters revealed that during the initial fever stage, the viral load was comparable for the patients who survived as well as the ones that died. Then in the second stage when multi-organ dysfunction occurred, from 7-13 days after disease onset, the viral load decreased in survivors but it remained high in the patients that died. The key risk factors that contributed to patient death were elevated serum aspartate aminotransferase, lactate dehydrogenase, creatine kinase, and creatine kinase fraction, as well as the appearance of CNS (central nervous system) symptoms, hemorrhagic manifestation, disseminated intravascular coagulation, and multi-organ failure. All clinical markers reverted to normal in the convalescent stage for SFTS patients who survived. Conclusions. We identified a period of 7-13 days after the onset of illness as the critical stage in SFTS progression. A sustained serum viral load may indicate that disease conditions will worsen and lead to death.
Background
Primary tumour location is emerging as an important prognostic factor in localized and metastatic colorectal cancers. However, its prognostic role in colorectal liver metastasis (CRLM) ...after hepatectomy remains controversial. A systematic review and meta‐analysis was undertaken to evaluate its prognostic value.
Methods
References were identified through searches of PubMed, Embase, Web of Science and the Cochrane Library comparing overall or disease‐free survival after hepatic resection between patients with CRLM originating from right‐ or left‐sided colorectal cancers. Data were pooled using hazard ratios (HRs) and 95 per cent confidence intervals according to a random‐effects model. Meta‐regression and subgroup analyses were conducted to assess the effect of underlying confounding factors on HR estimates and to adjust for this.
Results
The final analysis included 21 953 patients from 45 study cohorts. Compared with left‐sided primary tumour location, right‐sided location was associated with worse overall survival (HR 1·39, 95 per cent c.i. 1·28 to 1·51; P < 0·001; prediction interval 1·00 to 1·93), and also tended to have a negative impact on disease‐free survival (HR 1·18, 1·06 to 1·32; P = 0·004; prediction interval 0·79 to 1·75). Subgroup analysis showed that the negative effect of right‐sided primary tumour location on overall survival was more prominent in the non‐Asian population (HR 1·47, 1·33 to 1·62) than the Asian population (HR 1·18, 1·05 to 1·32) (P for interaction <0·01).
Conclusion
This study demonstrated a prognostic role for primary tumour location in patients with CRLM receiving hepatectomy, especially regarding overall survival. Adding primary tumour location may provide important optimization of prognosis prediction models for CRLM in current use.
Antecedentes
La ubicación del tumor primario (primary tumor location, PTL) ha surgido como un factor pronóstico importante en los cánceres colorrectales (colorectal cancers, CRCs) localizados y metastásicos. Sin embargo, todavía se discute su relevancia como factor pronóstico tras la resección de metástasis hepáticas de cáncer colorrectal (colorectal liver metastases, CRLM). Se realizó una revisión sistemática y un metaanálisis para determinar su valor pronóstico.
Métodos
En PubMed, EMBASE, Web of Science y la Biblioteca Cochrane se identificaron los trabajos que compararon la supervivencia global (overall survival, OS) y la supervivencia libre de enfermedad (disease‐free survival, DFS) tras la resección hepática de CRLM cuyo CRCs estuviese situado en el lado derecho o izquierdo. Los datos se expresaron en forma del cociente de riesgos instantáneos (hazard ratio, HR) e intervalos de confianza del 95% (i.c. del 95%) de acuerdo con un modelo de efectos aleatorios. Se efectuaron análisis de metarregresión y de subgrupos para evaluar el efecto de los factors de confusión existentes en las estimaciones de HR, ajustando por los mismos.
Resultados
El análisis final incluyó 21.953 pacientes de cohortes de 45 estudios. La PTL en el lado derecho en comparación con el lado izquierdo se asoció con una peor supervivencia global (HR 1,39; i.c. del 95% 1,28‐1,51; P < 0,001; intervalo de predicción 1,00‐1,93) y una tendencia a un impacto negativo en la DFS (HR 1,18; i.c. del 95% 1,06‐1,32; P = 0,004; intervalo de predicción 0,79‐1,75). El análisis de subgrupos mostró que el efecto negativo de la PTL del lado derecho en la OS fue más prominente en la población no asiática (HR 1,47; i.c. del 95% 1,33‐1,62) que en la asiática (HR 1,18; i.c. del 95% 1,05‐1,32; Pinteracción < 0,01).
Conclusión
Este estudio demostró que la PTL tiene un papel pronóstico tras la hepatectomía de las CRLM, especialmente respecto a la OS. La adición de la PTL proporcionaría una optimización importante en los modelos actuales de predicción pronóstica de CRLM.
The prognostic role of primary tumour location in colorectal liver metastases (CRLM) after hepatectomy remains controversial. This meta‐analysis demonstrated a prognostic role of primary tumour location in CRLM receiving hepatectomy, especially regarding overall survival.
Primary tumor location has prognostic value
Summary
Tertiary lymphoid structure (TLS) provides a local and critical microenvironment for both cellular and humoral immunity and supports effective antigen presentation and lymphocyte activation. ...However, the gene expression profile and prognostic significance of TLS in oral cancer remain largely unrevealed. In this study, we found the presence of both intratumoral and peritumoral TLSs in a series of 65 patients with oral cancer treated by surgical resection, with positive detection rates of 33.8 and 75.4%, respectively. The presence of intratumoral TLSs, but not peritumoral TLSs, was significantly associated with decreased P53 and Ki67 scores (P = 0·027 and 0·047, respectively). The survival analyses revealed that oral cancer patients with higher grades of TLSs was associated with improved disease‐free survival (DFS) and overall survival (OS) (P = 0·037 and 0·031, respectively). Gene expression profiling analysis of the cytokines and chemokines responsible for lymph‐node neogenesis identified a three‐up‐regulated‐gene set, i.e. IL7, LTB and CXCL13, which was shown to be correlated with human oral cancer‐associated TLSs. This study provides a framework for better understanding of oral cancer‐associated TLSs and for delineating future innovative prognostic biomarkers and immune therapeutic strategies for oral cancer.
Tertiary lymphoid structure (TLS) provides a local and critical microenvironment for both cellular and humoral immunity and supports effective antigen presentation and lymphocyte activation. Here, we found that oral cancer patients with higher grade TLSs signified improved disease‐free survival (DFS) and overall survival (OS). Gene expression profiling analysis of the cytokines and chemokines responsible for lymph‐node neogenesis identified a 3‐upregulated‐gene set, i.e. IL7, LTB and CXCL13, which was indicated correlated with oral cancer‐associated TLSs.
Resistance to cytotoxic chemotherapy drugs remains as the major cause of treatment failure in acute myeloid leukemia. Histone deacetylases (HDAC) are important regulators to maintain chromatin ...structure and control DNA damage; nevertheless, how each HDAC regulates genome stability remains unclear, especially under genome stress conditions. Here, we identified a mechanism by which HDAC3 regulates DNA damage repair and mediates resistance to chemotherapy drugs. In addition to inducing DNA damage, chemotherapy drugs trigger upregulation of HDAC3 expression in leukemia cells. Using genetic and pharmacological approaches, we show that HDAC3 contributes to chemotherapy resistance by regulating the activation of AKT, a well-documented factor in drug resistance development. HDAC3 binds to AKT and deacetylates it at the site Lys20, thereby promoting the phosphorylation of AKT. Chemotherapy drug exposure enhances the interaction between HDAC3 and AKT, resulting in decrease in AKT acetylation and increase in AKT phosphorylation. Whereas HDAC3 depletion or inhibition abrogates these responses and meanwhile sensitizes leukemia cells to chemotoxicity-induced apoptosis. Importantly, in vivo HDAC3 suppression reduces leukemia progression and sensitizes MLL-AF9
leukemia to chemotherapy. Our findings suggest that combination therapy with HDAC3 inhibitor and genotoxic agents may constitute a successful strategy for overcoming chemotherapy resistance.
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