Tissue culture and plantlet regeneration of the axillary bud’s leaf on spring cabbages Zhang Gaoxiang, Northernwest Agriculture and Forestry University, Yangling (China), College of Horticulture; Zhang Enhui, Northernwest Agriculture and Forestry University, Yangling (China), College of Horticulture; Xu Zhongmin, Northernwest Agriculture and Forestry University, Yangling (China), College of Horticulture
Xi bei nong lin ke ji da xue xue bao.Zi ran ke xue ban,
20/May., Letnik:
37, Številka:
5
Journal Article
探索一种能保留春季栽培春甘蓝亲本春性的育种方法。以春季田间栽培的春甘蓝MP01-_11152、GB9266-18136、DT409-05121 3个亲本自交系为试材，选用其成熟叶球腋芽叶片为外植体，首先以MS为基础培养基，添加0，2，3，4，5 mg/L 6-苄基腺嘌呤（6-BA）、0，0.05，0.1，0.2，0.3，0.4，0.5 mg/L ...α-萘乙酸（NAA）对MP01-11152亲本自交系进行筛选，筛选出6-BA和NAA的最佳质量浓度；然后利用已选出的6-BA和NAA最佳质量浓度，分别对供试3个品种进行腋芽叶片离体培养及植株再生研究。以MP01-11152为材料进行离体培养，筛选出6-BA和NAA的最佳质量浓度分别为2和0.4 mg/L。3个亲本自交系中，GB9266-18136在MS+2 mg/L 6-BA+0.4 mg/L NAA中诱导的不定芽分化率最高，为211%；MP01-11152次之，为122%；DT409-05121最低，为82%。将诱导出的不定芽在 MS+0.3 mg/L NAA生根培养基中诱导生根，不定根的诱导频率可达98%，并锻炼生长成苗。在MS培养基中添加适宜质量浓度的6-BA或NAA 2种激素，能够有效地提高春甘蓝腋芽叶片不定芽的诱导分化率，基因型对不定芽诱导的分化率有一定影响。
This passage discusses the method for keeping the springness from the leaf of the axillary buds on spring, to develop and enhance the breeding of spring cabbages. Three parental inbred lines MP01-11152，GB9266-18136, DT409-05121 were cultured in axillary bud’s leaves. A study on maximum plants regeneration was done in MS medium complementing to the material of MP01-11152, with MS as media by adding 6-BA with the concentration of 0, 2, 3, 4, 5 mg/L, then ad
Short chain fatty acids (SCFAs) are a class of saturated fatty acids containing 1-6 carbon atoms, which are mainly produced by specific flora in the intestine through the fermentation of dietary ...fiber, and play an important role in maintaining the homeostasis of the intestinal environment. Recent studies have shown that SCFAs can regulate glucose and lipid metabolism, regulate energy balance, maintain the intestinal barrier and reduce inflammatory responses, eventually participating in the occurrence and development of metabolic diseases such as type 2 diabetes mellitus, obesity, lipid metabolic disorders and nonalcoholic fat liver disease through the above multiple pathways. This article summarizes the mechanism of SCFAs regulating metabolism and the research progress in the prevention and treatment of metabolic diseases, in order to provide more reference materials for the prevention and treatment of metabolic diseases.
Saliency detection is essential to visual attention modelling and various computer vision tasks. Representation and measurement are two important issues for saliency models. Good representation and ...reasonable measurement are both critical issues in modelling visual saliency mechanism. For every input image, we obtain a self-adaptive dictionary that describes the image content effectively and image prior that forces sparsity in every location in the image using the K-SVD algorithm. For saliency measurement, background firing rate (BFR) is defined for each sparse features and it is followed by feature activation rate (FAR) computation to measure the bottom-up visual saliency.
Breast cancer is the second most common type of cancer and the leading cause of cancer death in women worldwide. Adjuvant tamoxifen therapy significantly slows down estrogen receptor （ER）-positive ...breast cancer progression, prolongs diseasefree survival, and contributes to the decrease in breast cancer mortality （Musgrove and Sutherland, 2009）. However, majority of the death from breast cancer is due to metastasis that is resistant to therapy （Musgrove and Sutherland, 2009）.
Immune cells, particularly macrophages, play critical roles in the hypoxia-induced inflammatory response. The small GTPase RhoB is usually rapidly induced by a variety of stimuli and has been ...described as an important regulator of cytoskeletal organization and vesicle and membrane receptor trafficking. However, it is unknown whether RhoB is involved in the hypoxia-induced inflammatory response. Here, we investigated the effect of hypoxia on the expression of RhoB and the mechanism and significance of RhoB expression in macrophages. We found that hypoxia significantly upregulated the expression of RhoB in RAW264.7 cells, mouse peritoneal macrophages, and the spleen of rats. Hypoxia-induced expression of RhoB was significantly blocked by a specific inhibitor of hypoxia-inducible factor-1α （HIF-1α）, c-Jun N-terminal kinase （JNK）, or extracellular-signal regulated protein kinase （ERK）, indicating that hypoxia-activated HIF-1α, JNK, and ERK are involved in the upregulation of RhoB by hypoxia. Knockdown of RhoB expression not only significantly suppressed basal production of interleukin-1 beta （IL-1β）, interleukin 6 （IL-6）, and tumor necrosis factor alpha （TNF-α） in normoxia but also more markedly decreased the hypoxia-stimulated production of these cytokines. Furthermore, we showed that RhoB increased nuclear factor-kappa B （NF-κB） activity, and the inhibition of NF-κB transcriptional activity significantly decreased the RhoB-increased mRNA levels of IL-1β, IL-6, and TNF-α. Finally, we demonstrated that RhoB enhanced cell adhesion and inhibited cell migration in normoxia and hypoxia. Taken together, these results suggest that RhoB plays an important role in the hypoxia-induced activation of macrophages and the inflammatory response.
At present, there are many types of drugs for clinical treatment of type 2 diabetes mellitus (T2DM) , there are differences in the hypoglycemic mechanisms and safety of traditional hypoglycemic drugs ...such as thiazolidinediones, sulfonylureas, biguanides, and insulin. In recent years, an assortment of new target drugs has emerged in an endless stream.New target drugs such as glucokinase activators, multi-site receptor agonists for GLP-1, GIP and glucagon, glucagon receptor inhibitors have gradually assembled the necessities of personalized clinical treatment through different mechanisms. This article reviews the current research progress of new target drugs for T2DM, and analyzes the advantages and disadvantages of clinical research of various new target drugs, in order to provide basic guidance for the clinical treatment of T2DM.
Human cytomegalovirus （HCMV） is a ubiquitous virus. Although the infection in healthy children and adults is usually asymptomatic, in immunocompromised individuals and newborns it is a significant ...cause of morbidity and mortality. UL49, an essential gene of HCMV, is highly conserved among various HCMV strains. The expression of UL49 is correlated with the production of virions. When UL49 is inhibited in the HCMV, the production of virions is reduced severely. In this study, RNA interference was applied to further investigate the roles of UL49 in viral replication. Two effective small interfering RNAs against UL49 were selected. Silencing of UL49 in HCMV-infected human foreskin fibroblast cells reduced the transcription levels of early and late genes, but not immediate-early ones. In addition, the viral DNA content was significantly reduced. This is the first time to uncover the role of UL49 in viral DNA synthesis, which indicates that UL49 might play an important role in this period. So the down-regulation of UL49 mRNA using RNAi might be a potential clinical therapy against the virus.
Background Recently, arsenic trioxide (As2O3) was considered as a novel anti-tumor agent. However, it showed severe toxicity effect on normal tissue at the same time. To improve its therapeutic ...efficacy and decrease its toxicity, we prepared arsenic trioxide-loaded albuminutes immuno-nanospheres As2O3-( HAS-NS)-BDI-1 targeted with nonoclonal antibody (McAb) BDI-1 and tested its specific killing effect against bladder cancer cell. Methods As2O3-HAS-NS was prepared by chemical cross-linking method. Monoclonal antibody BDI-1 was purified with ammonium sulphate sahingout and chromatography. Albuminutes microspheres were conjugated with McAb by SPDP cross-linking method. Concentration of As in As2O3-(HAS-NS)-BDI-1 and As2O3-HAS-NS was measured by atomic fluometry method. As2O3- (HAS-NS)-BDI-1 and its activity were detected by SDS-PAGE reduction electrophoresis, indirect immunofluorescence test, light microscope and scanning electron microscope observation. Acridine orange staining and tritiated thymidine (^3H-TdR) incorporation tests were used to indicate soecific killing activity of As2O3-(HAS-NS)-BDI-1 in vitro. Results In As2O3- (HAS-NS)-BDI-1 groups, we saw two protein bands in SDS-PAGE reduction electrophoresis. Albuminutes immuno-nanospheres were rounded with clear green fluorescence by immunofluorescence test. Under microscope, we observed that BIU-87 cells were covered with the As2O3-(HAS-NS)-BDI-1 and that As2O3- (HAS-NS)-BDI-1 moved with the BIU-87 cells. The albuminutes immunonanospheres were tightly junctioned with the BIU-87 cells. Specific killing activity of As2O3-(HAS-NS) -BDI-1 on bladder tumor cells was observed by acridine orange staining and ^3H-TdR incorporation assays. Conclusions As2O3- (HAS-NS)-BDI-1 might bind specifically against BIU-87 cells, thus leading to high activity of killing bladder tumor cells.
Agonist antibodies (Ab) directed against costimulatory molecules on the surface of antigen-primed T cells are in various stages of pre-clinical and clinical trials, albeit with limited therapeutic ...benefit as single agents. The underlying mechanisms of action remain incompletely understood. Here, we demonstrate an inhibitory role of ecto-enzyme CD73 for agonistic anti-4-1BB/CD137 Ab therapy. In particular, anti-4-1BB treatment preferentially drives CD73
effector T cell response for tumor inhibition. Anti-CD73 neutralizing Ab further improves anti-4-1BB therapy associated with enhanced anti-tumor T cell immunity. However, the TGF-β-rich tumor milieu confers resistance to anti-4-1BB therapy by sustaining CD73 expression primarily on infiltrating CD8
T cells across several tumor models. TGF-β blockade results in downregulation of CD73 expression on infiltrating T cells and sensitizes resistant tumors to agonistic anti-4-1BB therapy. Thus, our findings identify a mechanism of action for more effective clinical targeting of 4-1BB or likely other costimulatory molecules.
Emerging evidence has shown that dysregulation function of long non-coding RNAs (lncRNAs) implicated in gastric cancer (GC). However, the role of the differentially expressed lncRNAs in GC has not ...fully explained.
LncRNA expression profiles were determined by lncRNA microarray in five pairs of normal and GC tissues, further validated in another 75 paired tissues by quantitative real-time PCR (qRT-PCR). Overexpression of lncRNA MT1JP was conducted to assess the effect of MT1JP in vitro and in vivo. The biological functions were demonstrated by luciferase reporter assay, western blotting and rescue experiments.
LncRNA MT1JP was significantly lower in GC tissues than adjacent normal tissues, and higher MT1JP was remarkably related to lymph node metastasis and advance stage. Besides, GC patients with higher MT1JP expression had a well survival. Functionally, overexpression of lncRNA MT1JP inhibited cell proliferation, migration, invasion and promoted cell apoptosis in vitro, and inhibited tumor growth and metastasis in vivo. Functional analysis showed that lncRNA MT1JP regulated FBXW7 expression by competitively binding to miR-92a-3p. MiR-92a-3p and down-regulated FBXW7 reversed cell phenotypes caused by lncRNA MT1JP by rescue analysis.
MT1JP, a down-regulated lncRNA in GC, was associated with malignant tumor phenotypes and survival of GC. MT1JP regulated the progression of GC by functioning as a competing endogenous RNA (ceRNA) to competitively bind to miR-92a-3p and regulate FBXW7 expression. Our study provided new insight into the post-transcriptional regulation mechanism of lncRNA MT1JP, and suggested that MT1JP may act as a potential therapeutic target and prognosis biomarker for GC.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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