Two‐photon imaging is an emerging tool for biomedical research and clinical diagnostics. Electron donor–acceptor (D–A) type molecules are the most widely employed two‐photon scaffolds. However, ...current D–A type fluorophores suffer from solvatochromic quenching in aqueous biological samples. To address this issue, we devised a novel class of D–A type green fluorescent protein (GFP) chromophore analogues that form a hydrogen‐bond network in water to improve the two‐photon efficiency. Our design results in two‐photon chalcone (TPC) dyes with 0.80 quantum yield and large two‐photon action cross section (210 GM) in water. This strategy to form hydrogen bonds can be generalized to design two‐photon materials with anti‐solvatochromic fluorescence. To demonstrate the improved in vivo imaging, we designed a sulfide probe based on TPC dyes and monitored endogenous H2S generation and scavenging in the cirrhotic rat liver for the first time.
Turn bane into a boon: A unique class of two‐photon dyes with anti‐solvatochromic fluorescence, optically tunable groups, high fluorescence quantum yields, and large two‐photon action cross sections in water are developed. Their use for in vivo imaging, was demonstrated in the monitoring of endogenous H2S generation and scavenging in the cirrhotic rat liver.
•We introduce the theoretical guarantees of unsupervised domain adaptive re-ID based on 2. A DA-learnability result is shown under three assumptions that concerning the feature space. To the best of ...our knowledge, our paper is the first theoretical analysis work on domain adaptive re-ID tasks.•We theoretically turn the goal of satisfying the assumptions into tractable loss functions on the encoder network and data samples.•A self-training scheme is proposed to iteratively minimizing the loss functions. Our framework is applicable to all re-ID tasks and the effectiveness is verified on large-scale datasets for diverse re-ID tasks.
We study the problem of unsupervised domain adaptive re-identification (re-ID) which is an active topic in computer vision but lacks a theoretical foundation. We first extend existing unsupervised domain adaptive classification theories to re-ID tasks. Concretely, we introduce some assumptions on the extracted feature space and then derive several loss functions guided by these assumptions. To optimize them, a novel self-training scheme for unsupervised domain adaptive re-ID tasks is proposed. It iteratively makes guesses for unlabeled target data based on an encoder and trains the encoder based on the guessed labels. Extensive experiments on unsupervised domain adaptive person re-ID and vehicle re-ID tasks with comparisons to the state-of-the-arts confirm the effectiveness of the proposed theories and self-training framework. Our code is available on https://github.com/LcDog/DomainAdaptiveReID.
Real networks exhibit heterogeneous nature with nodes playing far different roles in structure and function. To identify vital nodes is thus very significant, allowing us to control the outbreak of ...epidemics, to conduct advertisements for e-commercial products, to predict popular scientific publications, and so on. The vital nodes identification attracts increasing attentions from both computer science and physical societies, with algorithms ranging from simply counting the immediate neighbors to complicated machine learning and message passing approaches. In this review, we clarify the concepts and metrics, classify the problems and methods, as well as review the important progresses and describe the state of the art. Furthermore, we provide extensive empirical analyses to compare well-known methods on disparate real networks, and highlight the future directions. In spite of the emphasis on physics-rooted approaches, the unification of the language and comparison with cross-domain methods would trigger interdisciplinary solutions in the near future.
•Omnichannel customer experience has been conceptualized as a multi-dimensional construct.•The key determinants of omnichannel customer experience have been identified.•Perceived compatibility and ...perceived risk mediate omnichannel experience and omnichannel shopping intention.
Advances in information and communication technologies (ICT) have led to the revolution in retail industry through integrating multiple available channels to enhance seamless customer experience, promoting a shift from multichannel to omnichannel business. This phenomenon has gained increasing attention in both academia and industry due to growing challenges to serve customers effectively. This study adopted a mixed-method approach to firstly conceptualize omnichannel customer experience and develop a survey instrument. Then, this study draws on the innovation diffusion theory to develop a nomological model that posits perceived compatibility and perceived risk as key linking mechanisms between omnichannel experience and omnichannel shopping intention. To achieve our research objective, we collected two data sets including pretest (n = 141) and model test (n = 377). We found that the constructs that represented our omnichannel experience conceptualization were good predictors of perceived compatibility and perceived risk, which further impact customers’ shopping intention. This study provides a rich conceptualization of an instrument for omnichannel customer experience that can serve as a springboard for future research to investigate the antecedents and impacts of omnichannel experience and can be used as a guide to design effective omnichannel retailing strategy.
Transforming acidic coiled-coil containing protein1 (TACC1) is closely related to transcription, translation and centrosome dynamics. Dysregulation of TACC1 is associated with multiple malignancies. ...Alternative splicing (AS) of TACC1 produces multiple variants, which are of great significance in cancer biology. However, the expression and biological functions of TACC1 variants in head and neck squamous cell carcinoma (HNSCC) remain unclear. In this study, we found for the first time that TACC1 variants exhibited a characteristic expression pattern and that TACC1 variant25 (TACC1v25) was downregulated in HNSCC tissues and cell lines. Overexpression of TACC1v25 in Cal27 and Fadu cells significantly inhibited proliferation and promoted autophagy. Moreover, expression levels of nuclear pERK and p-mTOR were significantly decreased, while the expression of Beclin-1 and the LC3II/LC3I ratio were increased in TACC1v25-overexpressed Cal27 and Fadu cells. After the addition of AKT activator SC79 to TACC1v25-overexpressed Cal27 and Fadu cells, the autophagy levels were remarkably rescued. In conclusion, TACC1v25 inhibits HNSCC progression through the ERK and AKT/mTOR pathways by inhibiting proliferation and increasing autophagy. TACC1v25 might have potential use as a tumour suppressor in HNSCC.
It is aimed at studying the role of parenting style on the career planning of vocational students in the context of information technology. This design takes the first-year and second-year students ...of two higher vocational colleges as the survey objects. From the perspective of educational psychology, firstly, the parenting style scale of these students is constructed, and meanwhile, the dimension design of career planning is carried out. Secondly, through the questionnaire survey, the career planning of students and the status quo of parenting styles are investigated, and the sample data are statistically analyzed by data analysis methods such as standard deviation analysis, cluster analysis, and correlation analysis. Finally, hypotheses are formulated and justified. The results reveal that the total score for career planning and the average score of each dimension are more than 3 points, indicating that vocational students have certain plans for their future careers. There are great differences in the gender of parenting styles, and the parental acceptance/involvement (t=3.389, p<0.01) dimension of the parenting style of the first-year students is greatly higher than that of the second-year students. On the dimension of psychological autonomy (t=−2.066, p<0.05), the score of second-year students is distinctly higher than that of the first year. There are obvious differences in grades from the dimensions of parental acceptance/participation and psychological autonomy. There is no observable difference in whether it is an only child (p>0.05) and the place of origin (p>0.05). In terms of the total score and each dimension of career planning, the authoritative type scored the highest, and the neglect type scored the lowest. Parents’ participation and acceptance behavior of vocational students are markedly related to their career planning. Parental punishment and acceptance are distinctly positively correlated with the career planning of these students and their two dimensions. Psychological autonomy is memorably associated with the domain of exploration.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, OILJ, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK, VSZLJ
Steroids are excreted from humans and animals and discharged with wastewaters into the environment, resulting in potential adverse effects on organisms. Based on the excretion rates from different ...groups of humans and animals, the emissions of seven steroids (estrone (E1), 17β-estradiol (E2), estriol (E3), testosterone (T), androsterone (A), progesterone (P), and cortisol (C)) were comprehensively estimated in 58 river basins of whole China, and their multimedia fate was simulated by using a level III fugacity multimedia model. The results showed that higher emission densities for the steroids were found in the river basins of east China than in west China. This distribution was found to be generally similar to the distribution of Gross Domestic Product (GDP) across China. E3, A, and P displayed higher emission densities than the other steroids in most of the river basins. The total excretion of steroids by humans and animals in China was estimated to be 3069 t/yr. The excretion of steroids from animals was two times larger than that from humans. After various treatments, the total emission of steroids was reduced to 2486 t/yr, of which more than 80% was discharged into the water compartment. The predicted concentrations in water were within an order of magnitude of the measured concentrations available in the literature. Owing to wastewater irrigation, more steroid mass loadings in agricultural soil were found in the basins of Haihe River and Huaihe River in comparison with the other river basins. To the best of our knowledge, this is the first report on the emissions and multimedia fate of seven steroids in the river basins of China.
► An method is developed for simultaneous determination of 11 classes of antibiotics. ► Water samples (pH 3, 0.2g Na2EDTA) were extracted by HLB cartridges. ► Solid samples were extracted using ...acetonitrile and citric buffer. ► The extracts were analyzed by RRLC–MS/MS.
A robust and sensitive analytical method is presented in which 11 classes of antibiotics are simultaneously extracted and determined in surface water, lagoon wastewater, influent, effluent, sediment, manure and sludge. Water samples with different volumes were adjusted to pH 3, added with 0.2g Na2EDTA and then extracted using Oasis hydrophilic–lipophilic balance (HLB) cartridges. Extraction of solid samples was carried out by a combination of ultrasonic and vortex mixing using a mixture of acetonitrile and citric buffer at pH 3 as the extraction solution. The extracts of the solid samples were then cleaned-up by a tandem solid phase extraction (SPE) method using a strong anion exchange cartridge (SAX) and a HLB cartridge, followed by analysis using rapid resolution liquid chromatography–tandem mass spectrometry (RRLC–MS/MS) equipped with electrospray ionization source. Among the 50 target compounds, the recoveries in the range of 50–150% were obtained for 39, 40, 36, 40, 38, 33 and 36 antibiotics in the spiked samples of surface water, lagoon wastewater, influent, effluent, sediment, manure and sludge with three concentrations, respectively. Method quantification limits (MQLs) for the target compounds (except sulfaguanidine and sulfanilamide) were in the range of 0.52–5.88ng/L, 2.36–65.8ng/L, 1.73–20ng/L, 1.42–9.52ng/L, 0.64–6.67ng/g (except bacitracin and cloxacillin), 1.33–17.4ng/g (except salinomycin, narasin, monensin, cloxacillin and novobiocin) and 1.50–28.6ng/g (except salinomycin, narasin, monensin and cloxacillin) in surface water, lagoon wastewater, influent, effluent, sediment, manure and sludge, respectively. The developed analytical method was successfully applied in the determination of target compounds in wastewater and sludge samples from Huiyang wastewater treatment plants, and in ground water, lagoon wastewater, manure and sediment collected from a pig farm, in South China.
The development of highly efficient bifunctional electrocatalysts for both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is crucial for improving the efficiency of overall ...water splitting, but still remains challenging issue. Herein, 3D self‐supported Fe‐doped Ni2P nanosheet arrays are synthesized on Ni foam by hydrothermal method followed by in situ phosphorization, which serve as bifunctional electrocatalysts for overall water splitting. The as‐synthesized (Ni0.33Fe0.67)2P with moderate Fe doping shows an outstanding OER performance, which only requires an overpotential of ≈230 mV to reach 50 mA cm−2 and is more efficient than the other Fe incorporated Ni2P electrodes. In addition, the (Ni0.33Fe0.67)2P exhibits excellent activity toward HER with a small overpotential of ≈214 mV to reach 50 mA cm−2. Furthermore, an alkaline electrolyzer is measured using (Ni0.33Fe0.67)2P electrodes as cathode and anode, respectively, which requires cell voltage of 1.49 V to reach 10 mA cm−2 as well as shows excellent stability with good nanoarray construction. Such good performance is attributed to the high intrinsic activity and superaerophobic surface property.
3D self‐supported Fe‐doped Ni2P nanosheet arrays are fabricated by hydrothermal method and in situ phosphorization. The performance of the nanosheet arrays toward overall water splitting depends on the Fe doping ratio. An alkaline electrolyzer using two identical (Ni0.33Fe0.67)2P nanosheet arrays electrodes can operate at 10 mA cm‐2 under 1.49 V.
The cycling stability of lithium metal batteries is steadily improving. The safety issues, which mainly result from the employment of flammable solvents, should be strongly considered for practical ...Li metal batteries. Nonflammable solvents can mitigate fire hazards; however, their employment irreversibly deteriorates the cycling stability of working batteries owing to intrinsic high reactivity against Li metal. Herein, regulating solvation structure in a dimethylacetamide (DMAC)‐based electrolyte is proposed to achieve compatibility between cycling stability and nonflammability of electrolytes. DMAC, a nonflammable solvent, is employed to construct a nonflammable localized high‐concentration electrolyte (LHCE). In the DMAC‐based LHCE, there are abundant aggregate clusters resulting in the formation of anion‐derived solid electrolyte interphase to circumvent parasitic reactions between DMAC solvents and Li metal and to improve the uniformity of Li deposition, which ensures the compatibility between cycling stability under practical conditions and nonflammability of electrolytes. This work opens an emerging avenue to construct long‐cycling and safe Li metal batteries by manipulating solvation structure in nonflammable electrolytes.
Regulating solvation structure in a dimethylacetamide‐based nonflammable electrolyte is explored to achieve compatibility between cycling stability and safety of electrolytes for practical lithium metal batteries. In the localized high‐concentration electrolyte, aggregate clusters are generated and result in the formation of an anion‐derived solid electrolyte interphase, and also improve the uniformity of Li deposition.
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