Is it possible to develop a quantitative method for detecting parental DNA contamination in conventional IVF using preimplantation genetic testing for aneuploidy (PGT-A)?
In this study, a ...quantification method was established for the parental contamination test (qPCT), which ensured more reliable results, and then verified its effectiveness for vitrified conventional IVF embryos. A total of 120 surplus vitrified blastocysts from patients who underwent prior routine IVF cycles were available for study.
The results of the prospective clinical study of qPCT-PGT-A showed that the maternal contamination rate was 0.83% (1/120) and that the risk of paternal contamination was negligible. The 24 frozen embryo transfer cycles resulted in 16 clinical pregnancies, including 13 live births, one late inevitable miscarriage and two ongoing pregnancies.
The risk of PGT in embryos with potential parental contamination is relatively low, and PGT-A is applicable for vitrified conventional IVF embryos.
Small-scale laboratory experiments of airflow through a single burner model and industrial-scale experiments on the centrally fuel-rich, low NOx swirl burner on a 300-MW wall-fired subcritical boiler ...burning low-volatile coal under deep air staging were performed. The aerodynamic characteristics, flue gas temperature, and gas concentrations were measured for various vane angles of outer secondary air in the burner nozzle region. The results show that a stable, symmetric central reverse-flow zone forms close to the exit of the burner nozzle region under deep air staging. With decreasing vane angle, i) the maximum axial, radial, and tangential velocities and swirl intensity of the airstream increase; ii) the decaying rate of velocity increases between X/D = 0 and X/D = 0.8; iii) the maximum diameter, length, and the jet divergence angle of the central reverse-flow zone increase; and iv) the relative reverse-flow rate increases. While the primary air concentration decreases slightly, the maximum primary air concentration decreases rapidly with decreasing vane angle. In contrast, the maximum axial relative mixing rate increases in the initial stage along the airstream direction. A decrease in vane angle increases the flue gas temperature, the rate of increase indicating a closer ignition position of the anthracite and lean coal along the airstream direction of the burner. The O2 consumption rate and NOx formation rate increase in the initial stage of combustion, whereas in the later stage the CO concentration increases notably and the O2 concentration remains almost constant below 1%. The CO concentration can exceed 20,000 ppm, which restrains the NOx formation and reduces the NOx concentration notably, but beyond X′ = 0.8 m, the NOx concentration remains almost constant. The flue gas temperature varies slightly for different vane angles in the side-wall region. The O2 concentration exceeds 4% near the location of the water-cooled wall. The O2 concentrations are below 2% and the CO concentrations are above 5000 ppm along the radial direction 1.8375 m ≤ R′≤2.3375 m from the centerline of the burner in the side-wall region. With decreasing vane angle, the CO concentration increases while the NOx concentration decreases.
•Under deep air staging a central reverse-flow zone is formed at the CFR burner exit.•As OSA vane angles decrease the swirl number and relative reverse-flow rate increase.•A reducing atmosphere is formed to inhibit NOx formation in primary combustion zone.•Under deep air staging CFR burner can reduce NOx formation of low volatile coal.
Adamantinomatous craniopharyngioma (ACP) is a neuroendocrine tumor whose pathogenesis remains unclear. This study investigated the role of glioma-associated oncogene family zinc finger 1 (GLI1), a ...transcription factor in the sonic hedgehog (SHH) signaling pathway, in ACP. We discovered that GLI1 regulates the expression of IL-6, thereby triggering inflammatory responses in ACP and influencing the tumor’s progression. Analyzing the Gene Expression Omnibus (GEO) database chip GSE68015, we found that GLI1 is overexpressed in ACP, correlating positively with the spite of ACP and inflammation markers. Knockdown of GLI1 significantly inhibited the levels of tumor necrosis factor α, interleukin-6 (IL-6), and IL-1β in ACP cells, as well as cell proliferation and migration. We further identified a binding site between GLI1 and the promoter region of IL-6, demonstrating that GLI1 can enhance the expression of IL-6. These findings were verified in vivo, where activation of the SHH pathway significantly promoted GLI1 and IL-6 expressions in nude mice, inducing inflammation and tumor growth. Conversely, GLI1 knockdown markedly suppressed these processes. Our study uncovers a potential molecular mechanism for the occurrence of inflammatory responses and tumor progression in ACP.
In this study, we aimed to explore the potential differences in proteomic profiles from the testicular tissue of azoospermatic men with impaired spermatogenesis and normal spermatogenesis. Isobaric ...tags for relative and absolute quantitation (iTRAQ) labeled technology and LC-MS/MS technology were used to identify differentially expressed proteins. Potential functions of differentially expressed proteins were predicted using gene ontology (GO) and the Kyoto encyclopedia of genes and genomes (KEGG). Immunohistochemistry (IHC) and western blot (WB) were used to verify the differentially expressed proteins. A protein-protein interaction (PPI) network was built to outline the regulatory network of differentially expressed proteins. A total of 3,945 proteins were identified in men with normal and impaired spermatogenesis. Of these, 116 proteins were differentially expressed in men with impaired spermatogenesis: 39 were upregulated and 77 were downregulated. Furthermore, we found that these differentially expressed proteins were mainly involved in the cellular component, which may be mainly associated with the spliceosome, ribosome, and thyroid hormone synthesis signaling pathways. The spliceosome- and ribosome-associated proteins YBX1, FBL, and HNRNPU were downregulated. And the proteomic profile of testicular tissue in men with impaired spermatogenesis is different from that of men with normal spermatogenesis. For this reason, differentially expressed proteins such as YBX1, FBL and HNRNPU might be involved in the pathology of spermatogenesis dysfunction.
Abbreviations: iTRAQ: Isobaric tags for relative and absolute quantitation;GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; IHC: Immunohistochemistry; WB: Western blot; PPI: Protein-protein interaction; ICSI: Intracytoplasmic sperm injection; BP: Biological process; CC: Cellular components; MF: Molecular function; snoRNA: Small nucleolar RNA; snRNA: Small nuclear RNA; LC-MS/MS: Liquid chromatography and MS/MS analysis; BSA: Bovine serum albumin; SD: Spermatogenic dysfunction; micro-TESE: Testicular microscopic sperm extraction
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method has been developed and validated for simultaneous quantification of ginsenosides Rg1, Re and notoginsenoside R1 ...in human plasma. Chromatography was performed on Capcell Pak C18 MG II column using a binary gradient using mobile phase A (5 mm ammonium formate solution) and B (methanol, containing 5 mm ammonium formate) at a flow rate of 0.3 mL/min. The entire chromatographic run time was 3.2 min. Quantification was achieved using multiple reaction monitoring in positive mode using API 3000. This method was validated in terms of specificity, linearity, precision, accuracy, matrix effect and stability. The calibration curves were linear in the concentration range of 0.020–5.00 ng/mL for ginsenosides Rg1, Re and notoginsenoside R1. The lower limit of quantification (LLOQ) of this method was 0.020 ng/mL. The intra‐run and inter‐run precision values were within 12.31% for ginsenoside Rg1, 14.13% for ginsenoside Re and 11.46% for notoginsenoside R1 at their LLOQ levels. The samples were stable under all tested conditions. This method was successfully applied to study the pharmacokinetics of ginsenoside Rg1 and notoginsenoside R1 in 24 healthy volunteers following oral administration of 200 mg Sanqi Tongshu Enteric‐Pellets Capsule.
Cold air experiments using a centrally fuel-rich pulverized-coal swirl burner model were performed within a laboratory-scale facility to study the effects of the outer secondary air cone length on ...the aerodynamic fields. The divergent angle of the air jet and the size of the central recirculation zone were found to increase with increasing outer secondary air cone lengths. The 0.5 MW pilot-scale facility was also used to optimize the length of the outer secondary air cone by measuring the flue gas temperature, and O2, CO and NOx concentrations, at different cone lengths. As the cone length was varied from 0 to 49 mm, the NOx emissions rapidly fell from 778 to 458 mg/m3 (O2 6%), while the unburned carbon content in the fly ash decreased from 5.83% to 3.51%, and CO concentration decreased from 1355 mg/m3 (O2 6%) to 99 mg/m3 (O2 6%). Based on these results, the optimum length of the outer secondary air cone was determined to be 49 mm.
•Effect of outer secondary air cone length on burner performance was determined.•The size of central recirculation zone increases with increasing the length.•NOx emissions and unburned carbon in fly ash decrease with increasing the length.•Considering NOx emissions and burnout rate, a length of 49 mm is advisable.
A rapid, specific, and sensitive liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method has been developed and validated to simultaneously quantify N‐acetyl‐p‐benzoquinoneimine (NAPQI), ...acetaminophen‐glutathione (acetaminophen‐glut) and acetaminophen‐glucuronide (acetaminophen‐gluc) in mouse plasma, liver and kidney homogenates. Analytes were eluted by a binary gradient mobile phase composed of water (phase A) and methanol containing 0.1% formic acid (phase B) at a flow rate of 0.3 mL/min, which was performed on a CAPCELL PAK C18 MG II column. It took 3.2 min to detect three analytes in a single run. Quantification was carried out in positive mode combined with multiple reaction monitoring. The validation of the LC‐MS/MS method consisted of specificity, linearity, precision, accuracy, protein precipitation recovery, matrix effect, dilution integrity and stability. The plasma and tissue homogenate calibration curves were linear over concentration ranges of 0.050–5.00, 0.050–5.00 and 0.100–40.0 μg/mL, with a lower limit of quantification of 0.050, 0.050, and 0.100 μg/mL for NAPQI, acetaminophen‐glut and acetaminophen‐gluc, respectively. The intra‐ and inter‐run precision values were within 12.47% for NAPQI, 12.11% for acetaminophen‐glut and 11.86% for acetaminophen‐gluc at their lower limit of quantitation levels. The samples were stable under all tested conditions. This method was successfully applied to study the pharmacokinetics of NAPQI, acetaminophen‐glut and acetaminophen‐gluc in ICR mice following oral administration of 200 mg/kg of acetaminophen suspension.
For three months in the summer of 2017 I did research on the journey of cyclists who ride China’s Route G318. This is an arduous, one-month, 2200 km., high altitude cycling journey from Chengdu to ...Lhasa. My study explores what the journey consists of, as well as the riders’ motivations for the journey, and the gender relationships that emerge during the journey. My data is primarily qualitative, gained from participant observation as a cyclist, in addition to interviews and questionnaires. Tourism itself is a phenomenon that has natural links to modernity. The special touristic journey is one way of constructing a self-identity through bodily movement. In my analysis I reflect on earlier research on existential authenticity and tourism reexamining it in the light of my own data. The research shows that the risks and bodily suffering from the journey is one way to demonstrate male cyclists’ masculinity which is hard to find in their regular lives, wherein female cyclists who complete the journey are usually viewed as “manly” women. I conclude that cycling G318 is a journey for challenging, enjoying, and contemplating one’s place in life, transcending oneself, and escaping modernity temporarily, through the efforts being made by one’s body as he or she completes the journey.
A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of ginsenosides Rg
, Re and notoginsenoside R
...in human plasma. Chromatography was performed on Capcell Pak C
MG II column using a binary gradient using mobile phase A (5 mm ammonium formate solution) and B (methanol, containing 5 mm ammonium formate) at a flow rate of 0.3 mL/min. The entire chromatographic run time was 3.2 min. Quantification was achieved using multiple reaction monitoring in positive mode using API 3000. This method was validated in terms of specificity, linearity, precision, accuracy, matrix effect and stability. The calibration curves were linear in the concentration range of 0.020-5.00 ng/mL for ginsenosides Rg
, Re and notoginsenoside R
. The lower limit of quantification (LLOQ) of this method was 0.020 ng/mL. The intra-run and inter-run precision values were within 12.31% for ginsenoside Rg
, 14.13% for ginsenoside Re and 11.46% for notoginsenoside R
at their LLOQ levels. The samples were stable under all tested conditions. This method was successfully applied to study the pharmacokinetics of ginsenoside Rg
and notoginsenoside R
in 24 healthy volunteers following oral administration of 200 mg Sanqi Tongshu Enteric-Pellets Capsule.
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