The objective of this study was to detect the Epstein-Barr virus (EBV) coinfection in coronavirus disease 2019 (COVID-19). In this retrospective single-center study, we included 67 COVID-19 patients ...with onset time within 2 weeks in Renmin Hospital of Wuhan University from January 9 to February 29, 2020. Patients were divided into EBV/SARS-CoV-2 coinfection group and SARS-CoV-2 infection alone group according to the serological results of EBV, and the characteristics differences between the two groups were compared. The median age was 37 years, with 35 (52.2%) females. Among these COVID-19 patients, thirty-seven (55.2%) patients were seropositive for EBV viral capsid antigen (VCA) IgM antibody. EBV/SARS-CoV-2 coinfection patients had a 3.09-fold risk of having a fever symptom than SARS-CoV-2 infection alone patients (95% CI 1.11-8.56; P = 0.03). C-reactive protein (CRP) (P = 0.02) and the aspartate aminotransferase (AST) (P = 0.04) in EBV/SARS-CoV-2 coinfection patients were higher than that in SARS-CoV-2 infection alone patients. EBV/SARS-CoV-2 coinfection patients had a higher portion of corticosteroid use than the SARS-CoV-2 infection alone patients (P = 0.03). We find a high incidence of EBV coinfection in COVID-19 patients. EBV/SARS-CoV-2 coinfection was associated with fever and increased inflammation. EBV reactivation may associated with the severity of COVID-19.
This paper discusses a single-machine scheduling problem with periodic maintenance activities and position-based learning effect to minimize the makespan. To obtain exact solutions of small-scale ...problems, one new two-stage binary integer programming model is formulated. In addition, a branch and bound algorithm combining boundary method and pruning rules is also proposed. According to the property of the optimal solution, a special search neighborhood is constructed. A hybrid genetic-tabu search algorithm based on genetic mechanism with tabu technique as an operator is proposed to solve medium-scale and large-scale problems. Moreover, to improve the efficiency of genetic algorithm and hybrid genetic-tabu search algorithm, Taguchi method is used for parameter tuning. Furthermore, computational experiments are carried out to compare the efficiency and performance of these algorithms.
•All the energy sources of the energy system of Tangshan have been chosen.•Flow analysis was used to trace the paths and to identify the nodes within network.•An information-based analysis was to ...quantify the overall stability of the network.•Measure for exploiting environmental-friendly energy sources was proposed.
Due to the huge energy demand and the influences from the technology, cost, production, and consumption structures, urban system has become a hot topic for the assessment of the energy security of energy systems. In this study, all the energy sources of the energy system of Tangshan, an industrial city that primarily depends on energy resources in Hebei Province, China, have been chosen as case study. Additionally, the metabolism theory from ecology is introduced to subdivide the sectors of system into producers, primary consumers, and secondary consumers and to trace the energy flows among them. The stability analysis includes the resilience and the restructuring capacity when facing disruptions and reflects the stability of the energy supply system and energy security. Flow and information-based analyses from ecological network analysis method are used to quantify the developing capacity, stability, and efficiency of the network from 2006 to 2016. The results indicate that that the heat supply, coking, gas generation, and coal products sectors were the providers of the integral energy of the system. In contrast, the industrial and household sectors were the receivers. The stability analysis indicated that, as the system’s resistance ability to disturbance grew, the system was more stable. However, the system itself still had specific problems including large energy consumption quantities from the industrial sector, and large heat consumption from the household sector. And this is related to the coastal area development and industrial restructure in Tangshan. Therefore, the analysis provides a tool for evaluating stability of an energy system from the perspective of energy supply.
Non-small cell lung cancer (NSCLC) accounts for approximately 85% of lung cancer cases, and it is one of the leading causes of cancer death in both men and women worldwide due to diagnosis in the ...advanced stage, rapid metastasis, and recurrence. At present, precision molecular targeted therapeutics directed toward NSCLC driven genes has made great progress and significantly improved the overall survival of patients with NSCLC, but can easily lead to acquired drug resistance. New methods are needed to develop real-time monitoring of drug efficacy and drug resistance, such as new molecular markers for more effective early detection and prediction of prognosis. Exosomes are nano-sized extracellular vesicles, containing proteins, nucleic acids and lipids, which are secreted by various cells, and they play an important role in the development of lung cancer by controlling a wide range of pathways. Tumor-derived exosomes are of great significance for guiding the targeted therapy of NSCLC and exosomes themselves can be a target for treatment. In this review, we describe the potential roles of tumor-derived exosomes and their clinical significance in NSCLC.
Developing suitable substrate materials is of significance in constructing electrochemical biosensors for fast and reliable quantification of molecules of chemical and biomedical interest. For ...practical applications, biosensors working at low negative potentials have the advantage of high selectivity and sensitivity. In this work, CeO2−x/C/rGO nanocomposites have been synthesized through the pyrolysis of metal organic frameworks with graphene oxide. The CeO2−x/C/rGO nanocomposites exhibit excellent catalytic properties towards H2O2, which is one of the uricase catalyzed intermediates at low working potentials due to the coexistence of Ce3+ and reduced graphene oxide (rGO). A novel biosensor based on the CeO2−x/C/rGO nanocomposites has been developed and utilized for the detection of uric acid, an important molecule in the biological and medical fields. The biosensor based on the CeO2−x/C/rGO nanocomposites presents a high sensitivity of 284.5 μA cm−2 mM−1 at −0.4 V (vs. SCE), a wide linear range between 49.8 and 1050.0 μM and a low detection limit of 2.0 μM. Moreover, it is found that the amperometric responses are free from interference of ascorbic acid and urea, which shows a great potential for practical applications.
Triple negative breast cancer (TNBC) is a heterogeneous disease and is highly related to immunomodulation. As we know, the most effective approach to treat TNBC so far is still chemotherapy. ...Chemotherapy can induce immunogenic cell death, release of damage-associated molecular patterns (DAMPs), and tumor microenvironment (TME) remodeling; therefore, it will be interesting to investigate the relationship between chemotherapy-induced TME changes and TNBC immunomodulation. In this review, we focus on the immunosuppressive and immunoreactive role of TME in TNBC immunomodulation and the contribution of TME constituents to TNBC subtype classification. Further, we also discuss the role of chemotherapy-induced TME remodeling in modulating TNBC immune response and tumor progression with emphasis on DAMPs-associated molecules including high mobility group box1 (HMGB1), exosomes, and sphingosine-1-phosphate receptor 1 (S1PR1), which may provide us with new clues to explore effective combined treatment options for TNBC.
Everolimus is a kind of mammalian target of rapamycin (mTOR) inhibitors. Activated mitogen-activated protein kinase interacting kinases/eukaryotic translation initiation factor 4E (MNK/eIF4E) axis ...plays a crucial role in resistance to Everolimus in non-small cell lung cancer (NSCLC). The eIF4E phosphorylation increased by mTOR inhibitors is mainly mediated by MNKs. However, the mechanisms are poorly understood. Recently, extensive reprogramming of miRNA profiles has also been found after long-term mTOR inhibitor exposure. Our previous studies have confirmed that tumor suppressor miR-7-5p is decreased in A549 cells after treatment with Everolimus. Exactly, MNK1 is the target of miR-7-5p. In this study, we investigated the biological functions and potential molecular mechanisms of miR-7-5p in the NSCLC undergoing treatment with Everolimus. We confirmed that Everolimus targeted mTORC1 inducing NSCLC cells to secrete miR-7-5p-loaded exosomes in Rab27A and Rab27B-dependent manners. Loss of intracellular miR-7-5p induced phosphorylation of MNK/eIF4E axis, but a supplement of extra exosomal miR-7-5p could reverse it. Of note, both low expression of miR-7-5p and elevated MNK1 protein were associated with a poor prognosis of NSCLC. Both endogenous miR-7-5p and exo-miR-7-5p enhanced the therapeutic efficacy of Everolimus by inhibiting the proliferation, migration, and metastasis of NSCLC in vitro and in vivo. The combination of miR-7-5p with Everolimus induced apoptosis to exhibit a synergistic anticancer therapeutic efficacy through dual abrogation of MNK/eIF4E and mTOR in NSCLC. In conclusion, Everolimus decreases the intracellular miR-7-5p by releasing of miR-7-5p loaded exosomes from NSCLC cells in Rab27A and Rab27B dependent manners. Either endogenous miR-7-5p or exo-miR-7-5p combined with Everolimus can enhance the anticancer efficacy by targeting MNK/eIF4E axis and mTOR. Besides, both low levels of miR-7-5p and positive expression of MNK1 act as independent poor prognostic biomarkers for NSCLC. Therefore, restoring miR-7-5p carried by exosome may be a promising novel combined therapeutic strategy with Everolimus for NSCLC.
MyD88 plays a central role in breast cancer, exerting a multitude of effects that carry substantial implications. Elevated MyD88 expression is closely associated with aggressive tumor ...characteristics, suggesting its potential as a valuable prognostic marker and therapeutic target. MyD88 exerts influence over several critical aspects of breast cancer, including metastasis, recurrence, drug resistance, and the regulation of cancer stem cell properties. Furthermore, MyD88 modulates the release of inflammatory and chemotactic factors, thereby shaping the tumor's immune microenvironment. Its role in immune response modulation underscores its potential in influencing the dynamic interplay between tumors and the immune system. MyD88 primarily exerts intricate effects on tumor progression through pathways such as Phosphoinositide 3-kinases/Protein kinase B (PI3K/Akt), Toll-like Receptor/Nuclear Factor Kappa B (TLR/NF-κB), and others. Nevertheless, in-depth research is essential to unveil the precise mechanisms underlying the diverse roles of MyD88 in breast cancer. The translation of these findings into clinical applications holds great promise for advancing precision medicine approaches for breast cancer patients, ultimately enhancing prognosis and enabling the development of more effective therapeutic strategies.
Cities consume 80% of the world׳s energy; therefore, analyzing urban energy metabolism and the resulting carbon footprint provides basic data for formulating target carbon emission reductions. While ...energy metabolism includes both direct and indirect consumptions among sectors, few researchers have studied indirect consumption due to a lack of data. In this study, we used input–output analysis to calculate the energy flows among directly linked sectors. Building on this, we used ecological network analysis to develop a model of urban energy flows and also account for energy consumption embodied by the flows among indirectly linked sectors (represented numerically as paths with a length of 2 or more). To illustrate the model, monetary input–output tables for Beijing from 2000 to 2010 were analyzed to determine the embodied energy consumption and associated carbon footprints of these sectors. This analysis reveals the environmental pressure based on the source (energy consumption) and sink (carbon footprint) values. Indirect consumption was Beijing׳s primary form, and the carbon footprint therefore resulted mainly from indirect consumption (both accounting for ca. 60% of the total, though with considerable variation among sectors). To reduce emissions, the utilization efficiency of indirect consumption must improve.
•We quantified the embodied energy transfers among Beijing׳s socioeconomic sectors.•We calculated the sectors׳ intensity of energy consumption and carbon footprint.•The indirect energy consumption was higher than the direct for all sectors.•The high-indirect-consumption sectors are at the end of industrial supply chains.•High-indirect-consumption sectors can improve upstream products energy efficiency.
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