This paper analyzes the interactions between three dimensions of firm performance – productivity, energy efficiency, and environmental performance – and especially sheds light on the role of ...environmental management. In this context, environmental management is investments to reduce environmental impact, which may also affect firm competitiveness, in terms of change in productivity, and spur more (or less) efficient use of energy. We apply data envelopment analysis (DEA) technique to calculate the Malmquist firm performance indexes, and a panel vector auto-regression (VAR) methodology is utilized to investigate the dynamic and causal relationship between the three dimensions of firm performance and environmental investment. Main results show that energy efficiency and environmental performance are integrated, and energy efficiency and productivity positively reinforce each other, signifying the cost saving property of more efficient use of energy. Hence, increasing energy efficiency, as advocated in many of today's energy policies, could capture multiple benefits. The results also show that improved environmental performance and environmental investments constrain next period productivity, a result that would be in contrast with the Porter hypothesis and strategic corporate social responsibility; both concepts conveying the notion that pro-environmental management can boost productivity and competitiveness.
•We analyze three dimensions of firm performance and investigates the role of environmental management (EM).•We use DEA to calculate firm performance and panel VAR to assess the relationship between performances and EM.•Energy efficiency (EE) and environmental performance (EP) are integrated, and EE and productivity (P) support each other.•Improved EP and EM seem to constrain next period P.
Integration of functional nanomaterials with optical micro/nanofibers (OMNFs) can bring about novel optical properties and provide a versatile platform for various sensing applications. OMNFs as the ...key element, however, have seldom been investigated. Here, we focus on the optimization of fiber diameter by taking micro/nanofiber-based localized surface plasmon resonance sensors as a model. We systematically study the dependence of fiber diameter on the sensing performance of such sensors. Both theoretical and experimental results show that, by reducing fiber diameter, the refractive index sensitivity can be significantly increased. Then, we demonstrate the biosensing capability of the optimized sensor for streptavidin detection and achieve a detection limit of 1 pg/mL. Furthermore, the proposed theoretical model is applicable to other nanomaterials and OMNF-based sensing schemes for performance optimization.
Coal mining machine drums are prone to damage and malfunction under extremely complex working conditions, which seriously affects the efficiency and safety of coal production. In this paper, based on ...the theory of coal rock cutting and virtual simulation technology, finite element models of drum cutting coal rock were established and then verified by physical experiments. Through simulation analysis, the dynamic reliability of the drum was studied from three aspects: load, stress and wear, and a mathematical model of drum load was established with respect to the traction speed and drum rotation speed; based on the orthogonal test, the optimal working parameters to improve the wear resistance of the drum were derived. The results of the study found that when the traction speed increases, the load on the drum increases, and when the drum rotation speed increases, the load on the drum decreases; when the traction speed is increased from 2 to 6 m/min, the stress on the pick body under different rotation speeds increases to different degrees, with an average increase rate of 27.394%; when the drum rotation speed is 90 r/min, the traction speed is 3 m/min, and the coal loading mode is projectile loading, the wear depth of the picks and spiral blades is relatively small. The research method and results of this paper can provide a reference for the selection of the drum working parameters.
•We propose the Vernier effect induced by the birefringence in fiber-optic modal interferometers for sensitivity enhancement.•We experimentally demonstrate an ultrahigh RI sensitivity of 35,823.3 ...nm/RIU at low RI of 1.333 using a fiber coupler with the Vernier effect.•We apply this sensor for human cardiac troponin T detection, and a LOD of 1 ng/mL is achieved.
We report a simple and effective method to improve the sensitivities of fiber-optic modal interferometers with birefringence induced Vernier effect. Taking optical microfiber coupler as an example, we study the sensitivity enhancement of a microfiber coupler for refractive index (RI) sensing both theoretically and experimentally in the RI range of 1.33–1.35 where bioassays are typically carried out. Numerical results show that by tracing the wavelength shifts of dips in the envelope formed by the Vernier effect, RI sensitivities can be improved by almost one order of magnitude compared to the sensors without the Vernier effect. Then we experimentally achieve an ultra-high sensitivity of 35,823.3 nm/RIU using a microfiber coupler with a width of 3.2 μm. More importantly, we apply this ultra-sensitive sensor to detect human cardiac troponin, and a limit of detection of 1 ng/mL is achieved. This sensor is simple in configuration andcan serve as a bio-photonic platform for clinical diagnostics environmental monitoring and food safety.
The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), the most lethal brain tumor. However, the ...molecular mechanisms underlying this crosstalk are incompletely understood. Here, we show that GSCs secrete the Wnt-induced signaling protein 1 (WISP1) to facilitate a pro-tumor microenvironment by promoting the survival of both GSCs and tumor-associated macrophages (TAMs). WISP1 is preferentially expressed and secreted by GSCs. Silencing WISP1 markedly disrupts GSC maintenance, reduces tumor-supportive TAMs (M2), and potently inhibits GBM growth. WISP1 signals through Integrin α6β1-Akt to maintain GSCs by an autocrine mechanism and M2 TAMs through a paracrine manner. Importantly, inhibition of Wnt/β-catenin-WISP1 signaling by carnosic acid (CA) suppresses GBM tumor growth. Collectively, these data demonstrate that WISP1 plays critical roles in maintaining GSCs and tumor-supportive TAMs in GBM, indicating that targeting Wnt/β-catenin-WISP1 signaling may effectively improve GBM treatment and the patient survival.
Brain tumor initiating cells (BTICs) co-opt the neuronal high affinity glucose transporter, GLUT3, to withstand metabolic stress. We investigated another mechanism critical to brain metabolism, ...mitochondrial morphology, in BTICs. BTIC mitochondria were fragmented relative to non-BTIC tumor cell mitochondria, suggesting that BTICs increase mitochondrial fission. The essential mediator of mitochondrial fission, dynamin-related protein 1 (DRP1), showed activating phosphorylation in BTICs and inhibitory phosphorylation in non-BTIC tumor cells. Targeting DRP1 using RNA interference or pharmacologic inhibition induced BTIC apoptosis and inhibited tumor growth. Downstream, DRP1 activity regulated the essential metabolic stress sensor, AMP-activated protein kinase (AMPK), and targeting AMPK rescued the effects of DRP1 disruption. Cyclin-dependent kinase 5 (CDK5) phosphorylated DRP1 to increase its activity in BTICs, whereas Ca(2+)-calmodulin-dependent protein kinase 2 (CAMK2) inhibited DRP1 in non-BTIC tumor cells, suggesting that tumor cell differentiation induces a regulatory switch in mitochondrial morphology. DRP1 activation correlated with poor prognosis in glioblastoma, suggesting that mitochondrial dynamics may represent a therapeutic target for BTICs.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SBMB, UILJ, UKNU, UL, UM, UPUK
The gas chromatography–ion mobility spectrometry (GC-IMS) method is a new technology for detecting volatile organic compounds. This study was carried out to evaluate the effects of volatile aroma ...compounds of Curcuma essential oils (EOs) after 60Co radiation by GC-IMS. Dosages of 0, 5, and 10 kGy of 60Co were used to analyze EOs of Curcuma after 60Co irradiation (named EZ-1, EZ-2, and EZ-3). The odor fingerprints of volatile organic compounds in different EOs of Curcuma samples were constructed by headspace solid-phase microextraction and GC-IMS after irradiation. The differences in odor fingerprints of EOs were compared by principal component analysis (PCA). A total of 92 compounds were detected and 65 compounds were identified, most of which were ketones, aldehydes, esters, and a small portion were furan compounds. It was found that the volatile matter content of 0 kGy and 5 kGy was closer, and the use of 10 kGy 60Co irradiation would have an unstable effect on the EOs. In summary, it is not advisable to use a higher dose when using 60Co irradiation for sterilization of Curcuma. Due to the small gradient of irradiation dose used in the experiment, the irradiation dose can be adjusted appropriately according to the required sterilization requirements during the production and storage process of Curcuma to obtain the best irradiation conditions. GC-IMS has the advantages of GC’s high separation capability and IMS’s fast response, high resolution, and high sensitivity, and the sample requires almost no pretreatment; it can be widely used in the analysis of traditional Chinese medicines containing volatile components. It is shown that irradiation technology has good application prospects in the sterilization of traditional Chinese medicines, but the changes in irradiation dose and chemical composition must be paid attention to.
Osteogenic differentiation of human bone marrow–derived mesenchymal stem cells (BMSCs) has been regarded as a central issue in fracture healing. MicroRNAs (miRNAs, miRs) participate in diverse ...physiological processes such as osteoblastic differentiation of BMSCs. In this study, we found that miR-664a-5p was upregulated during osteogenic differentiation of human BMSCs, and this upregulation positively correlated with the expression of osteogenic genes Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN). Overexpression of miR-664a-5p promoted the osteogenic differentiation of BMSCs, whereas a knockdown of miR-664a-5p suppressed it. Additionally, high-mobility group A2 (HMGA2) mRNA was identified as a direct target of miR-664a-5p that mediates the function of this miRNA. Overexpression of HMGA2 obviously attenuated miR-664a-5p–induced osteogenic differentiation of BMSCs. Thus, the newly identified miR-664a-5p–HMGA2 pathway expands our understanding of the mechanisms underlying the osteogenic differentiation of human BMSCs, may provide deeper insights into the regulation of this differentiation, and can point to new effective methods for treating osteoporosis.
•MiR-664a-5p expression correlates with the osteogenic differentiation of human BMSCs.•Overexpression of miR-664a-5p promotes the osteogenic differentiation of human BMSCs.•Knockdown of miR-664a-5p suppresses the osteogenic differentiation of BMSCs.•HMGA2 mRNA is a target of miR-664a-5p.•MiR-664a-5p negatively regulates HMGA2 expression during osteogenic induction.
Intense infiltration of tumour-associated macrophages (TAMs) facilitates malignant growth of glioblastoma (GBM), but the underlying mechanisms remain undefined. Herein, we report that TAMs secrete ...abundant pleiotrophin (PTN) to stimulate glioma stem cells (GSCs) through its receptor PTPRZ1 thus promoting GBM malignant growth through PTN-PTPRZ1 paracrine signalling. PTN expression correlates with infiltration of CD11b
/CD163
TAMs and poor prognosis of GBM patients. Co-implantation of M2-like macrophages (MLCs) promoted GSC-driven tumour growth, but silencing PTN expression in MLCs mitigated their pro-tumorigenic activity. The PTN receptor PTPRZ1 is preferentially expressed in GSCs and also predicts GBM poor prognosis. Disrupting PTPRZ1 abrogated GSC maintenance and tumorigenic potential. Moreover, blocking the PTN-PTPRZ1 signalling by shRNA or anti-PTPRZ1 antibody potently suppressed GBM tumour growth and prolonged animal survival. Our study uncovered a critical molecular crosstalk between TAMs and GSCs through the PTN-PTPRZ1 paracrine signalling to support GBM malignant growth, indicating that targeting this signalling axis may have therapeutic potential.
Glioblastomas are highly lethal cancers, containing self-renewing glioblastoma stem cells (GSC). Here, we show that GSCs, differentiated glioblastoma cells (DGC), and nonmalignant brain cultures all ...displayed robust circadian rhythms, yet GSCs alone displayed exquisite dependence on core clock transcription factors, BMAL1 and CLOCK, for optimal cell growth. Downregulation of
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in GSCs induced cell-cycle arrest and apoptosis. Chromatin immunoprecipitation revealed that BMAL1 preferentially bound metabolic genes and was associated with active chromatin regions in GSCs compared with neural stem cells. Targeting
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attenuated mitochondrial metabolic function and reduced expression of tricarboxylic acid cycle enzymes. Small-molecule agonists of two independent BMAL1-CLOCK negative regulators, the cryptochromes and REV-ERBs, downregulated stem cell factors and reduced GSC growth. Combination of cryptochrome and REV-ERB agonists induced synergistic antitumor efficacy. Collectively, these findings show that GSCs co-opt circadian regulators beyond canonical circadian circuitry to promote stemness maintenance and metabolism, offering novel therapeutic paradigms. SIGNIFICANCE: Cancer stem cells are highly malignant tumor-cell populations. We demonstrate that GSCs selectively depend on circadian regulators, with increased binding of the regulators in active chromatin regions promoting tumor metabolism. Supporting clinical relevance, pharmacologic targeting of circadian networks specifically disrupted cancer stem cell growth and self-renewal.
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