The Caryophyllaceae family is complex. Several attempts have been carried out in the past to study Caryophyllaceae members. This study mainly focused on Allochrusa Bunge to determine its genetic ...structure and used ISSR markers, ITS, and rps16 data to classify and differentiate Allochrusa species. We collected 122 Allochrusa specimens. Our analysis included morphological and molecular method approaches. Morphometry analysis indicated that floral characters could assist in the identification of Allochrusa species. A. persica (Boiss.) Boiss. and A. versicolor Fisch. & C.A.Mey. showed affinity to each other. A. bungei Boiss. formed a separate group. Analysis of molecular variance showed significant genetic differentiation in Allochrusa (p= 0.001). The majority of genetic variation was among the Allochrusa population. We recorded minimum gene flow (Nm=0.176) between Allochrusa species. Besides this, isolation by distance occurs in Allochrusa members, as shown in the Mantel test result (r = 0.01, p = 0.0002). STRUCTURE analysis revealed three genetic groups. It is evident that A. persica, A. versicolor, and A. bungei differ genetically from each other. Our current findings have implications in plant systematics and biodiversity management.
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the ...relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.
China is in the process of integrating the new cooperative medical scheme (NCMS) and the urban residents' basic medical insurance system (URBMI) into the urban and rural residents' basic medical ...insurance system (URRBMI). However, how to integrate the financing policies of NCMS and URBMI has not been described in detail. This paper attempts to illustrate the differences between the financing mechanisms of NCMS and URBMI, to analyze financing inequity between urban and rural residents and to identify financing mechanisms for integrating urban and rural residents' medical insurance systems.
Financing data for NCMS and URBMI (from 2008 to 2015) was collected from the China health statistics yearbook, the China health and family planning statistics yearbook, the National Handbook of NCMS Information, the China human resources and social security statistics yearbook, and the China social security yearbook. "Ability to pay" was introduced to measure inequity in health financing. Individual contributions to NCMS and URBMI as a function of per capita disposable income was used to analyze equity in health financing between rural and urban residents.
URBMI had a financing mechanism that was similar to that used by NCMS in that public finance accounted for more than three quarters of the pooling funds. The scale of financing for NCMS was less than 5% of the per capita net income of rural residents and less than 2% of the per capita disposable income of urban residents for URBMI. Individual contributions to the NCMS and URBMI funds were less than 1% of their disposable and net incomes. Inequity in health financing between urban and rural residents in China was not improved as expected with the introduction of NCMS and URBMI. The role of the central government and local governments in financing NCMS and URBMI was oscillating in the past decade.
The scale of financing for URRBMI is insufficient for the increasing demands for medical services from the insured. The pooling fund should be increased so that it can better adjust to China's rapidly aging population and epidemiological transitions as well as protect the insured from poverty due to illness. Individual contributions to the URBMI and NCMS funds were small in terms of contributors' incomes. The role of the central government and local governments in financing URRBMI was not clearly identified. Individual contributions to the URRBMI fund should be increased to ensure the sustainable development of URRBMI. Compulsory enrollment should be required so that URRBMI improves the social medical insurance system in China.
Change detection is of great significance in remote sensing. The advent of high-resolution remote sensing images has greatly increased our ability to monitor land use and land cover changes from ...space. At the same time, high-resolution remote sensing images present a new challenge over other satellite systems, in which time-consuming and tiresome manual procedures must be needed to identify the land use and land cover changes. In recent years, deep learning (DL) has been widely used in the fields of natural image target detection, speech recognition, face recognition, etc., and has achieved great success. Some scholars have applied DL to remote sensing image classification and change detection, but seldomly to high-resolution remote sensing images change detection. In this letter, faster region-based convolutional neural networks (Faster R-CNN) is applied to the detection of high-resolution remote sensing image change. Compared with several traditional and other DL-based change detection methods, our proposed methods based on Faster R-CNN achieve higher overall accuracy and Kappa coefficient in our experiments. In particular, our methods can reduce a large number of false changes.
To identify the role of YAP in cisplatin resistance in human ovarian cancer cells and in the regulation of autophagy in these cancer cells.
The cisplatin-sensitive OV2008 parental cell line and its ...cisplatin-resistant variant C13K were cultured. RNA interference was used to knock down the YAP gene. Accumulation of GFP-LC3 puncta was performed by fluorescence microscopy. The formation of autophagosomes was observed by transmission electron microscopy. Drug sensitivity was examined using CCK-8 assay, while apoptosis, the level of intracellular rhodamine 123 and lysosomal acidification were analyzed by fluorescence-activated cell sorting. Acid phosphatase activity was measured using an acid phosphatase-assay kit. Real-time polymerase chain reaction, Western blotting, and immunofluorescence detection were used to detect the protein and messenger RNA expression of YAP, YAP target genes, CCND1, cleaved PARP, and caspase 3, Atg-3 and -5, and the LC3B protein.
YAP signaling may regulate cisplatin resistance in ovarian cancer cells by augmenting cellular autophagic flux. After knockdown of YAP-sensitized C13K cells to cisplatin by inducing a decrease in autophagy, YAP led to an increase in autophagy via enhancement of autolysosome degradation.
YAP-mediated autophagy may play a protective role in cisplatin-resistant human ovarian cancer cells. Therefore, YAP-mediated autophagy should be explored as a new target for enhancing the efficacy of cisplatin against ovarian cancer and other types of malignancies.
In recent years, with the rapid development of the sports industry, the quality of sports training products on the market is uneven. Problems such as inaccurate detection of athletes’ physical ...indicators, low comfort of sportswear, and reduced satisfaction with sports equipment often occur. To this end, this article proposes to apply conjugated materials with excellent optical, electrical, thermal and other properties to sports training and sports products, by summarizing the properties of conjugated materials and their applications in sports training, explores the potential of conjugated materials in improving athletes’ training effects, monitoring sports status, and improving sports equipment. This article rates the application of conjugated materials in sports training products in terms of comfort, waterproofness, portability, lightness, aesthetics and breathability. The results showed that the average scores of the 20 sports participants on sportswear were 9.0475, 9.0075, 9.01, 9.025, 9.0325 and 9.04 respectively; the average scores on sports shoes were 9.035, 9.055, 9.02, 9.085, 9.0175 and 8.9975 respectively. Research shows that applying conjugated materials to sports training can improve athletes’ performance and contribute to the better development of sports.
Timely and accurate data with high resolutions holds great promise for more responsible and advanced power grid operations. The research has been focusing on design of monitoring and control scheme ...given the assumptions that the supporting information and communication technology (ICT) systems are capable of providing data and perform control with sufficient quality. A relatively less addressed aspect is the dependency of wide-area monitoring and control (WAMC) systems on their supporting ICT architecture which is usually a compromise between various concerns, such as data quality, interoperability, or security. Without an appropriate ICT architecture design, the projected WAMC system functionalities run the risk of being jeopardized. This paper begins with a presentation about possible delays brought by complex data-transfer and processing processes. Analytical experiments are conducted with purposes to quantify the maximum delay and input signal's sensitivity toward delay on a typical WAMC application where the control of static var compensation (SVC) is coordinated with generator excitations using phasor measurements. Given the characteristics of this particular control scheme, two possible ICT architectures that provide data with different qualities are compared concerning the reliability of this WAMC application. This paper concludes by proposing a generic ICT architecture, enabling efficient WAMC systems implementation in terms of data quality.
Selenium isotopes are becoming an important paleoenvironmental proxy. However, few studies have focused on the behavior of Se isotopes during oxidative weathering. In this paper, a comprehensive set ...of Se isotopic composition and concentration data were collected from the weathering profiles of Se-rich shales of the Permian Maokou Formation in Yutangba and Shadi, China to investigate Se isotopic fractionation and Se enrichment during weathering processes. The δ82/76Se in fresh shales (148±118mg/kg Se, 1SD, n=40) from Shadi and Yutangba drill cores varies from −1.69‰ to 1.74‰ with an average of 0.40±0.71‰ (1SD, n=40), consistent with the range in other Phanerozoic shales, suggesting that Se isotopes are not strongly fractionated during Se sequestration in the primary sedimentary environment. However, the strongly weathered Se-rich shales from Shadi and Yutangba profiles are isotopically lighter with average δ82/76Se values of −1.96±1.08‰ (1SD, n=5) and −1.08±1.83‰ (1SD, n=23), respectively. These data suggest that Se isotopes can be fractionated during oxidation and reduction processes associated with weathering, with heavier isotopes removed preferentially during oxidative weathering of shales. Such a shift, if found to be a global phenomenon, would have implications for models of the global Se cycle and interpretation of Se isotope data from past biogeochemical regimes.
Locally altered shales exposed in a quarry at Yutangba are extremely enriched in Se with 1642±1505mg/kg (1SD, n=45), approximately 10 times greater than that in unaltered drill core samples. These rocks display very strong variation in δ82/76Se over short distances, with a single 60cm transect showing the most strongly negative and positive δ82/76Se values (−14.20‰ to +11.37‰) observed to date in natural samples. This suggests that Se has undergone multiple cycles of oxidation, mobilization, and re-reduction, resulting in a Se-rich redox front that has migrated downward through the organic-rich shales over time. δ82/76Se values vary sharply over distances as small as 10cm, indicating that Se redox conditions change strongly with position and are controlled by fractures and rock layering. Our data and a simple conceptual model suggest that zones of increased permeability that are accessed first by infiltrating waters are isotopically light, whereas less accessible zones are heavy. Repeated redox cycling accentuates this pattern. Furthermore, the average δ82/76Se in Se-rich shales at the Yutangba weathering system is 0.45±5.77‰ (1SD, n=39), identical with that (0.40±0.71‰) in fresh shales from same locality, implying that Se released by weathering accumulates in the redox front with little loss. The strong Se isotopic fractionation occurring in the redox front implies that Se isotopes can be extensively used in tracing geochemical processes of Se in groundwater system, especially related to fractures.
•First identification of volcanic-related U deposit in the EKOB.•Uranium mineralization is contemporaneous with the Triassic diabase.•Pitchblende with high temperature-like REE patterns may result ...from relatively highly saline Cl-bearing fluids.•U mineralization results from intense fluid-rock interaction and fluid boiling.
The Haidewula deposit in Northwest China is a recently discovered volcanic-related uranium deposit. To determine the origin of this deposit, whole-rock geochemical, stable isotope, U–Pb isotope and trace element compositions of the pitchblende were obtained, and microthermometric analyses of the fluid inclusions were performed. The Haidewula deposit is located within the Phanerozoic volcanic basin and contains Silurian trachyte, rhyolite, rhyolitic tuff, and Triassic basalt. These Silurian volcanic rocks are characterized by high-K calc-alkaline properties and were intruded by Triassic dolerite, which served as a source for mineralization. The mineralized zones were primarily influenced by NE-NNE-trending and SE-dipping faults. Vein-like pitchblende is commonly associated with calcite, illite, quartz, pyrite, and fluorite. Geochronology indicated that uranium mineralization occurred during 235–230 Ma. The ore-forming fluids contained meteoric water with limited magmatic water and exhibited moderate to low temperatures (246–133 °C), moderate to low salinities (17.3–1.06 wt% NaCleqv) and Cl-bearing rocks. U precipitation was attributed to changes in physicochemical conditions resulting from fluid–rock interactions and fluid boiling. The uranium mineralization at Haidewula coincided with the evolution of the Tethys Ocean. During late Wenlock, an extensional environment, triggered by the closure of the Proto-Tethys Ocean, led to the extrusion of Haidewula rhyolite and trachyte. During the crustal extensional environment resulting from the subduction of the A’nyemaqen Ocean in the Middle Triassic, ore-forming fluids circulated and remobilized uranium from the Haidewula volcanic rocks. As a result of fluid–rock interactions and fluid boiling within brittle structures, the U-complexes destabilized and formed pitchblende at Haidewula.
Chitin is a major component of the exoskeleton and the peritrophic matrix of insects. It forms complex structures in association with different assortments of cuticle and peritrophic matrix proteins ...to yield biocomposites with a wide range of physicochemical and mechanical properties. The growth and development of insects are intimately coupled with the biosynthesis, turnover, and modification of chitin. The genes encoding numerous enzymes of chitin metabolism and proteins that associate with and organize chitin have been uncovered by bioinformatics analyses. Many of these proteins are encoded by sets of large gene families. There is specialization among members within each family, which function in particular tissues or developmental stages. Chitin-containing matrices are dynamically modified at every developmental stage and are under developmental and or physiological control. A thorough understanding of the diverse processes associated with the assembly and turnover of these chitinous matrices offers many strategies to achieve selective pest control.
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