Nowdays, the Walt Disney Company is one of the most influential entertainment companies in the world, which is famous for its creativity and unique marketing strategy.Disney used to be just a small ...animation studio but now it has influence all over the world. To investigate the key factors which made Disney’s successful global expansion and development,this paper did this following reserach. This research is mainly about Disney’s core business and the marketing strategy used by Disney. The study was conducted through a meticulous analysis of Disney’s primary business segments, its overarching business strategies, and its management practices. Furthermore, it examines Disney’s brand positioning, market penetration tactics, and customer engagement techniques that collectively forge its competitive advantage. By dissecting the synergy between Disney’s content creation and its promotional tactics, this research aims to unravel the intricacies of Disney’s approach in nurturing a loyal consumer base and securing its status as an indomitable force in the entertainment industry.
A novel magnetic molecularly imprinted sensing film (MMISF) was fabricated for the determination of estradiol (E2) based on magnetic glassy carbon electrode (MGCE) and magnetic molecularly imprinted ...polymers (MMIPs). The MMIPs were synthesized by in situ polymerization of glutathione (GSH)-functionalized gold (Au)-coated Fe3O4 (Fe3O4@Au-GSH) nanocomposites and aniline. The MMISF was constructed with MMIPs via a kind of “soft modification” where MMIPs were assembled and immobilized on the surface of MGCE or removed from it by freely installing a magnet into MGCE or not. The E2-MMIPs were obtained by MMIPs recognizing E2 from sample, and the electrochemical detection was carried out after forming the “soft modification” sensing film by putting MGCE into the E2-MMIPs suspension liquid. Afterwards, the “soft modification” MMISF was peeled off from the electrode by removing the magnet from MGCE. The interface of the electrode could be quickly refreshed through simple treatment for the next detection. The structures and morphologies of Fe3O4@Au-GSH, MMIPs and MMISF were investigated by Fourier transform infrared spectrometer, ultraviolet and visible spectrophotometer, scanning electron microscope and atomic force microscope. In addition, the MMISF was successfully used for detecting E2 in milk powder with good sensitivity, selectivity, reproducibility and efficiency. The linear range of the MMISF for E2 was 0.025–10.0μmolL−1 with the limit of detection of 2.76nmolL−1 (S/N= 3).
•A well-ordered magnetic molecularly imprinted sensing film (MMISF) was fabricated by magnetic field directed self-assembly via a kind of “soft modification” under external magnetic field.•The magnetic molecularly imprinted polymers (MMIPs) based on Fe3O4@Au-GSH showed good molecular recognition and excellent electrochemical activity of estradiol.•The “soft modification” MMISF could be freely constructed or removed by using a controllable magnet, which avoided the complicated surface refreshing and modification of the electrodes.•The preparation of MMIPs, the recognition, detection and elution were completed independently, which might improve the efficiency of the analysis and meet the need of large scale analysis.
Transcription factors (TFs) regulate gene expression by binding to regulatory regions, and their dysregulation is involved in numerous diseases. Thus, they are therapeutic targets and potential ...diagnostic markers. However, widely used methods for TFs detection are either cumbersome or costly. Herein, we first applied DNA-Ag nanoclusters molecular beacons (AgMBs) in TFs analysis and designed an assay based on the switchable fluorescence of AgMBs. In the absence of TFs, a single-stranded DNA functioned as a reporter is released from a double-stranded DNA probe (referred as dsTFs probe) under exonuclease III (Exo III) digestion. Then, the reporter triggers downstream Exo III-assisted signal amplification by continuously consuming the guanine-rich enhancer sequences in AgMBs, resulting in significant fluorescent decrease eventually. Conversely, the presence of TFs protects the dsTFs probe from digestion and blocks the downstream reaction to keep a highly fluorescent state. To testify this rationale, we utilized nuclear factor-kappa B p50 (NF-κB p50) as a model TFs. Owing to the amplification strategy, this method exhibited high sensitivity toward NF-κB p50 with a limit of detection of 10 pM, and a broad linear range from 30 pM to 1.5 nM. Furthermore, this method could detect multiple TFs in human colon cancer DLD-1 cells and reflect the variation in their cellular levels after stimulation. Finally, by conducting an inhibition assay we revealed the potential of this method for screening TFs-targeted drugs and calculating the IC50 of corresponding inhibitors.
As a solvent, ethanol can extract a wide range of polar substances, but its consumption is sometimes avoided for religious and cultural reasons. In this study, liquefied dimethyl ether (DME) was used ...to extract curcumin and antioxidants from highly moist, untreated turmeric. Higher amounts of curcumin (7.94 mg/g dry weight (DW)) were extracted using liquified DME compared with ethanol (6.77 mg/g DW). Almost all the water and 5.10 mg/g DW of lipids were extracted from raw turmeric using liquefied DME, corresponding to 56 % the amount extracted using ethanol. In addition, microscopic and spectroscopic analyses revealed that liquefied DME neither destroyed cell walls nor extracted cellulose. However, liquefied DME had a slightly lower extraction capacity for total phenolic compounds than ethanol and slightly lower antioxidant effect. DME extracts an equivalent amount of curcumin as ethanol and only slightly fewer antioxidants while simultaneously avoiding sun-drying degradation and prolonged freeze-drying.
Inflammation can cause various chronic diseases like inflammatory bowel diseases. Various food protein-derived bioactive peptides (BAPs) with anti-inflammatory activity have the potential to manage ...these diseases. The aim of this paper is to overview the mechanisms and the molecular targets of BAPs to exert anti-inflammatory activity. In this review, the in vitro and in vivo effects of BAPs on intestinal inflammation are highlighted. The mechanism, pathways, and future perspectives of BAPs as the potential sources of therapeutic treatments to alleviate intestinal inflammation are provided, including nuclear factor-κB, mitogen-activated protein kinase, Janus kinase-signal transducer and activator of transcription, and peptide transporter 1 (PepT1), finding that PepT1 and gut microbiota are the promising targets for BAPs to alleviate the intestinal inflammation. This review provides a comprehensive understanding of the role of dietary BAPs in attenuating inflammation and gives a novel direction in nutraceuticals for people or animals with intestinal inflammation.
Based on magnetic field directed self-assembly (MDSA) of Fe3O4@rGO composites, a novel magnetic molecularly imprinted electrochemical sensor (MIES) was fabricated and developed for the determination ...of the azo dye amaranth. Fe3O4@rGO composites were obtained by a one-step approach involving the initial intercalating of iron ions between the graphene oxide layers via the electrostatic interaction, followed by the reduction with hydrazine hydrate to deposit Fe3O4 nanoparticles onto the reduced oxide graphene nanosheets. In molecular imprinting, the complex including the function monomer of aniline, the template of amaranth and Fe3O4@rGO was pre-assembled through π–π stacking and hydrogen bonding interactions, and then was self-assembled on the surface of magnetic glassy carbon electrode (MGCE) with the help of magnetic field induction before electropolymerization. The structures and morphologies of Fe3O4@rGO and the doped molecularly imprinted polymers (MIPs) were investigated by Fourier transform infrared spectrometer (FT-IR), Raman spectra and scanning electron microscope (SEM). Besides, the characterization by differential pulse voltammetry (DPV) showed that Fe3O4@rGO composites promoted the electrical conductivity of the imprinted sensors when doped into the MIPs. The adsorption isotherms and adsorption kinetics were employed to evaluate the performances of MIES. The detection of amaranth was achieved via the redox probe K3Fe(CN)6 by blocking the imprinted cavities, which avoided the interferences of oxidation products and analogs of amaranth. Furthermore, the prepared MIES exhibited good sensitivity, selectivity, reproducibility and efficiency for detecting amaranth in fruit drinks. The average recoveries were 93.15–100.81% with the RSD <3.0%.
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•Fe3O4@rGO could enhance the sensitivity of the imprinted sensor.•The doping Fe3O4@rGO could be easily immobilized by magnetic field induction.•The thickness of imprinted membrane could be controlled by external field.•The sensor could avoid the interferences of oxidation products and analogs.
A novel electrochemical sensing platform based on magnetic field-induced self-assembly of Fe3O4@Polyaniline nanoparticles (Fe3O4@PANI NPs) has been for the first time fabricated for the sensitive ...detection of creatinine in biological fluids. The template molecule, creatinine, was self-assembled on the surface of Fe3O4@PANI NPs together with the functional monomer aniline by the formation of N–H hydrogen bonds. After pre-assembled, through the magnetic-induction of the magnetic glassy carbon electrode (MGCE), the ordered structure of molecularly imprinted polymers (MIPs) were established by the electropolymerization and assembled on the surface of MGCE with the help of magnetic fields by a simple one-step approach. The structural controllability of the MIPs film established by magnetic field-induced self-assembly was further studied. The stable and hydrophilic Fe3O4@PANI can not only provide available functionalized sites with which the template molecule creatinine can form hydrogen bond by the abundant amino groups in PANI matrix, but also afford a promoting pathway for electron transfer. The as-prepared molecularly imprinted electrochemical sensor (MIES) shows good stability and reproducibility for the determination of creatinine with the detection limit reached 0.35nmolL−1 (S/N=3). In addition, the highly sensitive and selective MIES has been successfully used for the clinical determination of creatinine in human plasma and urine samples. The average recoveries were 90.8–104.9% with RSD lower than 2.7%.
•MIES was prepared based on magnetic field-induced self-assembly by a one-step approach.•Fe3O4@PANI NPs were oriented and assembled orderly on the surface of MGCE, which enhanced the sensitivity of the electrode.•The sensor was successfully used for rapid, sensitive and selective determination of creatinine in human plasma and urine samples.
A dual-mode sensor was developed for detecting acetylcholinesterase (AChE) and organophosphorus pesticides (OPs) via bifunctional BSA-CeO
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nanoclusters (NCs) with oxidase-mimetic activity and ...fluorescence property. The dual-mode sensor has the characteristics of self-calibration and self-verification, meeting the needs of different detection conditions and provide more accurate results. The colorimetric sensor and fluorescence sensor have been successfully used for detecting AChE with limit of detection (LOD) of 0.081 mU/mL and 0.056 mU/mL, respectively, while the LOD for OPs were 0.9 ng/mL and 0.78 ng/mL, respectively. The recovery of AChE was 93.9–107.2% and of OPs was 95.8–105.0% in actual samples. A novel strategy was developed to monitor pesticide residues and detect AChE level, which will motivate future work to explore the potential applications of multifunctional nanozymes.
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Ti3C2/TiO2 hybrids are environment-friendly and exhibit excellent photocatalytic and hydrogen-generating power characteristics. Herein, a novel single-step method is proposed for fabricating ...multilayer structures in which TiO2, generated from (NH4)2TiF6, wraps the Ti3C2 MXene by etching Ti3AlC2 with (NH4)2TiF6. The optimal reaction conditions for the etching of Ti3AlC2 with (NH4)2TiF6 were systematically studied. The phase composition, morphology, and photophysical properties of the Ti3C2/TiO2 hybrids were investigated using X-ray diffraction, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and UV–vis spectrophotometry. The thermal stability of the hybrids was investigated using thermogravimetric and differential thermal analyses. Along with the formation of Ti3C2 MXene, Ti3AlC2 reacted with (NH4)2TiF6 at 60 °C for 24 h to form hybrids surrounded by NH4TiOF3 crystals. Subsequent reactions of these hybrids with H3BO3 resulted in the conversion of NH4TiOF3 crystals into TiO2 and eventually into Ti3C2/TiO2 hybrids. Furthermore, the photocatalytic activity of the Ti3C2/TiO2 hybrids was measured by monitoring the photodegradation of methylene blue under ultraviolet light, which showed that the photocatalytic activity of the Ti3C2/TiO2 hybrids was higher than that of the commercial anatase TiO2 nanoparticles.
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•A novel synthesis of Ti3C2 MXene using (NH4)2TiF6 as an etching agent.•TiO2 formed from (NH4)2TiF6 can be hybridized with Ti3C2 MXene.•This method is hydrofluoric acid-free, and the composite has a fine structure.•The composite exhibited good photocatalytic performance.
Experience value is positively associated with user voice toward social media, but existing research lacks an examination of its mechanisms of action. Based on value co-creation theory, this paper ...explores the relationship between experience value (i.e., social value, entertainment value, information value) and customer voice, and explains the specific influence mechanism through the mediating role of user loyalty. The results of the empirical tests show that social value, entertainment value and information value have significant effects on user loyalty; user loyalty has a significant effect on promotive voice but not on prohibitive voice; user loyalty mediates the relationship between body social value, entertainment value, information value and promotive voice. The findings of this research reveal the important role of experience value on customer voice, which is an important guide for social media to achieve sustainable development.
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