We present spectroscopic observations of 182 disk galaxies (96 in the cluster and 86 in the field environment) in the region of the Abell 901/902 multiple cluster system, which is located at a ...redshift of \(z\sim 0.165\). The presence of substructures and non-Gaussian redshift distributions indicate that the cluster system is dynamically young and not in a virialized state. We find evidence for two important galaxy populations. \textit{Morphologically distorted galaxies} are probably subject to increased tidal interactions. They show pronounced rotation curve asymmetries at intermediate cluster-centric radii and low rest-frame peculiar velocities. \textit{Morphologically undistorted galaxies} show the strongest rotation curve asymmetries at high rest-frame velocities and low cluster-centric radii. Supposedly, this group is strongly affected by ram-pressure stripping due to interaction with the intra-cluster medium. Among the morphologically undistorted galaxies, dusty red galaxies have particularly strong rotation curve asymmetries, suggesting ram pressure is an important factor in these galaxies. Furthermore, dusty red galaxies on average have a bulge-to-total ratio higher by a factor of two than cluster blue cloud and field galaxies. The fraction of kinematically distorted galaxies is 75% higher in the cluster than in the field environment. This difference mainly stems from morphological undistorted galaxies, indicating a cluster-specific interaction process that only affects the gas kinematics but not the stellar morphology. Also the ratio between gas and stellar scale length is reduced for cluster galaxies compared to the field sample. Both findings could be best explained by ram-pressure effects.
Sixteen murine hybridoma-secreting monoclonal antibodies against pancreatic islet cell surface antigens (mc-ICSA) have been produced by the cell fusion technique using splenocytes from xenogeneic ...islet cell- or RIN cell-immunized Balb/c mice. In addition, some mice were autoimmunized by subdiabetogenic doses of the beta cell toxin streptozotocin in combination with complete Freund's adjuvant. Isotyping of the mc-ICSA revealed that 13 of the antibodies belong to the class IgM, and 3 to the subclass IgG1. The specificity of these mc-ICSA has been detected by means of the indirect immunofluorescent technique using primary rat islet cells suspensions, following a procedure of double immunostaining for pancreatic insulin and glucagon. Furthermore, the cross reactivity of these mc-ICSA was studied using endothelial, neuroblastoma and fibroblast cell lines and primary rat splenocytes. One out of the 10 mc-ICSA tested was alpha cell-specific, 2 were beta cell-specific and 7 out of 10 were reactive with both alpha and beta cells. Eleven mc-ICSA showed no cross-reactivity with the 5 other cell types tested. The binding of one mc-ICSA was blocked by 6 of the ICSA-positive human sera which were tested, suggesting that this monoclonal recognizes the same antigenic determinant. The same mc-ICSA diminished the glucose- and arginine-stimulated insulin secretion of isolated rat islets.
Islet cell antibodies have been detected in more than 60% of newly diagnosed type I diabetics. Their pathogenetic role is still unclear. We have generated monoclonal antibodies (mc-ab) reactive with ...islet cell antigens by fusing mouse myeloma cells with spleen cells from Balb/c mice immunized with pancreatic islet cells. Hybridomas producing islet cell surface antibodies (ICSA) were detected by indirect immunofluorescence on viable cells from rat islets or rat insulinoma. Cytoplasmic islet cell antibodies (ICA) were detected by indirect immunofluorescence on Bouin-fixed sections of mouse pancreas. The ICSA- and/or ICA-producing hybridomas were cloned twice by limiting dilution. This paper describes six different mc-ab. All hybrid cell lines obtained produced IgM antibodies. Four of them mediate complement-dependent cytotoxicity to viable rat islet cells. In the present study the heterogeneity of circulating ICSA is demonstrated. Also, a monoclonal beta cell surface autoantibody K56aF3 was produced by fusion of spleen cells from a mouse treated with sub-diabetogenic doses of streptozotocin in combination with complete Freund's adjuvant. It was cytotoxic against islet cells up to a dilution of 1:1,000 and it could inhibit the insulin secretion from neonatal rat islets cultured in RPMI 1640 as stimulated by glucose or by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine common with glucose. The latter effect was reversible as indicated by the recovery of insulin secretion in a subsequent culture period without mc-ab. These results suggest that circulating ICSA in type I diabetics may alter beta cell function and thereby contribute to the pathogenesis of type I diabetes.
We describe a method to efficiently obtain two-dimensional velocity fields of distant, faint and small, emission-line galaxies with FORS2 at the VLT. They are examined for kinematic substructure to ...identify possible interaction processes. Numerical simulations of tidal interactions and ram-pressure effects reveal distinct signatures observable with our method. We detect a significant fraction of galaxies with irregular velocity fields both in the field and cluster environments.
Innovationen im Bereich wissensbasierter Diagnostik Ziegler, Susanne; Schwingeler, Sabine; Malsch, Thomas ...
Zeitschrift für wirtschaftlichen Fabrikbetrieb,
03/2022, Letnik:
94, Številka:
5
Magazine Article
Recenzirano
Abstract
Die Störungssuche, Fehlerdiagnose und Reparatur komplexer technischer Anlagen konfrontieren das zuständige Personal mit hohen Anforderungen. Wissensbasierte Diagnosesysteme waren bisher kaum ...in der Lage, die Maschinenexperten bei der Akquisition komplexen anlagendiagnostischen Wissens angemessen zu unterstützen. Im Rahmen der Implementierung eines wissensbasierten Diagnose- und Informationssystems in einem Maschinenbauunternehmen hat eine interdisziplinäre Forschungsgruppe neue Werkzeuge und Konzepte zur System- und Organisationsentwicklung erarbeitet.
Innovationen im Bereich wissensbasierter Diagnostik Ziegler, Susanne; Schwingeler, Sabine; Malsch, Thomas ...
Zeitschrift für wirtschaftlichen Fabrikbetrieb,
03/2022, Letnik:
94, Številka:
5
Magazine Article
Recenzirano
Die Störungssuche, Fehlerdiagnose und Reparatur komplexer technischer Anlagen konfrontieren das zuständige Personal mit hohen Anforderungen. Wissensbasierte Diagnosesysteme waren bisher kaum in der ...Lage, die Maschinenexperten bei der Akquisition komplexen anlagendiagnostischen Wissens angemessen zu unterstützen. Im Rahmen der Implementierung eines wissensbasierten Diagnose- und Informationssystems in einem Maschinenbauunternehmen hat eine interdisziplinäre Forschungsgruppe neue Werkzeuge und Konzepte zur System- und Organisationsentwicklung erarbeitet.
Immunoperoxidase staining and enzyme-linked immunosorbent assay (ELISA) were used to identify monoclonal antibodies that reacted with pancreatic islet cells. All monoclonal antibodies produced ...against isolated human or rat pancreatic islets including one mouse autoantibody reacted with pancreatic islets in formalin-fixed pancreas sections, but not with rat kidney or thyroid. Reactivity was also found with suspensions of normal rat islet cells and rat insulinoma cells using a 3-stage immunoperoxidase procedure and an ELISA technique. Differences were observed in staining intensity between the various antigenic substrates tested suggesting variable cross-reactivity and/or number of epitopes. The sensitivity of the immunoperoxidase technique proved to be favourable for identification of monoclonal antibodies that recognize cellular constituents such as islet cell antigens present in low concentrations.
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