Tumor-derived exosomes and their contents promote cancer metastasis. Phosphoglycerate mutase 1 (PGAM1) is involved in various cancer-related processes. Nevertheless, the underlying mechanism of ...exosomal PGAM1 in prostate cancer (PCa) metastasis remains unclear. In this study, we performed in vitro and in vivo to determine the functions of exosomal PGAM1 in the angiogenesis of patients with metastatic PCa. We performed Glutathione-S-transferase pulldown, co-immunoprecipitation, western blotting and gelatin degradation assays to determine the pathway mediating the effect of exosomal PGAM1 in PCa. Our results revealed a significant increase in exosomal PGAM1 levels in the plasma of patients with metastatic PCa compared to patients with non-metastatic PCa. Furthermore, PGAM1 was a key factor initiating PCa cell metastasis by promoting invadopodia formation and could be conveyed by exosomes from PCa cells to human umbilical vein endothelial cells (HUVECs). In addition, exosomal PGAM1 could bind to γ-actin (ACTG1), which promotes podosome formation and neovascular sprouting in HUVECs. In vivo results revealed exosomal PGAM1 enhanced lung metastasis in nude mice injected with PCa cells via the tail vein. In summary, exosomal PGAM1 promotes angiogenesis and could be used as a liquid biopsy marker for PCa metastasis.
Chronic pancreatitis (CP) is a chronic inflammatory disease of the pancreas. This study aimed to compare the natural course of alcoholic chronic pancreatitis (ACP) and idiopathic chronic pancreatitis ...(ICP).
CP patients admitted to our center from January 2000 to December 2013 were enrolled. Characteristics were compared between ACP and ICP patients. Cumulative rates of diabetes mellitus (DM), steatorrhea, pancreatic stone, pancreatic pseudocyst, biliary stricture, and pancreatic cancer after the onset and the diagnosis of CP were calculated, respectively. The cumulative rates of DM and steatorrhea after diagnosis of pancreatic stone were also calculated.
A total of 2,037 patients were enrolled. Among them, 19.8% (404/2,037) were ACP and 80.2% (1,633/2,037) were ICP patients. ACP and ICP differs in many aspects, especially in gender, age, smoking, complications, morphology of pancreatic duct, and type of pain. The development of DM, steatorrhea, PPC, pancreatic stone, and biliary stricture were significantly earlier and more common in ACP patients. No significant difference was observed for pancreatic cancer development. There was a rather close correlation between exocrine/endocrine insufficiency and pancreatic stone in ACP patients, which was much less correlated in ICP patients.
The long-term profile of ACP and ICP differs in some important aspects. ACP patients usually have a more severe course of CP. These differences should be recognized in the diagnosis and treatment of CP.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
High-purity (∼96%) isolated s-SWCNT film with narrow diameter distribution were prepared on large scale by oxygen-assisted FCCVD method.
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•Bulk isolated s-SWCNT films with a high ...content of 96% were selectively prepared.•O2 was found to play function on catalyst particle size and s-SWCNT diameter.•FET devices show good performance verifying the enrichment of s-SWCNTs.
Semiconducting single-wall carbon nanotubes (s-SWCNTs) are promising for use in flexible electronics as a channel material. However, it remains a big challenge to directly grow high purity, high-quality s-SWCNTs in large scale. Here we report the synthesis of isolated s-SWCNTs by an oxygen-assisted floating catalyst chemical vapor deposition method. By controlling the density of nucleated SWCNTs, isolated or small-bundled, rather than large-bundled SWCNTs were generated in a floating state, so that the oxygen introduced could more efficiently selectively etch the metallic-SWCNTs formed. In addition, it was found that the oxygen also functions in limiting the size of Fe catalyst nanoparticles in a narrow range of 5–8 nm. As a result, isolated s-SWCNTs with a narrow diameter distribution were synthesized. The content of s-SWCNTs reached ∼96%, and the percentage of isolated tubes was estimated to be ∼83%. Thin-film transistors (TFTs) constructed using the s-SWCNT film showed high on/off ratios ranging from 2.1 × 104 to 1.2 × 106, verifying the effective enrichment of s-SWCNTs.
Single-wall carbon nanotubes (SWCNTs) with ultra-thin channels are considered promising nanoreactors for confined catalysis, chemical reactions, and drug delivery. The fabrication of SWCNT ...nanoreactors by cutting usually suffers from low efficiency and poor controllability. Here we develop a defect-induced gas etching method to efficiently cut SWCNTs and to obtain nanoreactors with ultrasmall confined space. H
2
plasma treatment was performed to generate defects in the walls of SWCNTs, then H
2
O vapor was used as a "knife" to cut SWCNTs at the defect sites, and short cut-SWCNTs with an average length of 175 nm were controllably obtained with a high yield of 75% under optimized conditions. WO
3
@SWCNT derivatives with different morphologies were synthesized using short cut-SWCNTs as nanoreactors. The radiation resistance of WO
3
@SWCNT hybrids improved obviously, thus providing a platform for the synthesis of novel SWCNT-based derivatives with fascinating properties.
A defect-induced H
2
O etching method was proposed to efficiently cut single-wall carbon nanotubes (SWCNTs) controllably. Short SWCNTs are promising nanoreactors for the efficient synthesis of SWCNT-based derivatives with novel properties.
Oxidative N‐heterocyclic carbene (NHC) organocatalysis, typically leading to the formation of acyl azolium reactive intermediates, constitutes one of the most important activation strategies for the ...NHC‐catalyzed chemical transformations. Here, we report an unprecedented oxidative radical NHC catalysis by using peroxyester as the external single‐electron oxidant to realize divergent difunctionalization of olefins. The key to success of this chemistry is the catalytic generation of oxygen radicals that could trigger an intermolecular hydrogen atom transfer to activate the inert C−H bonds, thereby enabling the productive radical relay process. With this protocol, commonly used general chemicals could serve as radical precursors to allow efficient synthesis of value‐added products in a straightforward and cost‐effective manner. Preliminary mechanistic investigations, including control experiments and DFT calculations, shed light on the NHC organocatalytic radical reaction mechanism.
An unprecedented oxidative radical NHC catalytic system is reported that allows an efficient difunctionalization of olefins for the divergent synthesis of various ketones. Key to success is the use of peroxyesters as “dual‐role” reagents to achieve a sequential single‐electron oxidation and intermolecular hydron atom transfer.
Person re-identification is a challenging research issue in computer vision and has a broad application prospect in intelligent security. In recent years, with the emergence of large-scale person ...datasets and the rapid development of deep learning, many outstanding results have been achieved in person re-identification researches, which mainly involves two critical technologies: feature extraction and distance metric. Among them, feature extraction has been well summarized in the current literature of person re-identification, but there is no systematic analysis of the distance metric method in the current review literature. However, effective and reliable distance metric is crucial to improve the accuracy of person re-identification. Therefore, it is necessary to systematically review and summarize the metric learning methods in person re-identification, so as to provide some references for the researchers of metric learning. In this paper, we make a comprehensive analysis of metric learning methods in the past five years, which can be summarized into three aspects: distance metric method, metric learning algorithm, and re-ranking for the metric results. Then, we compare the performance of some representative metric learning methods and discuss them in-depth. Finally, we make a prospect for the future research direction of metric learning in person re-identification.
Risk of pancreatic cancer may increase in chronic pancreatitis patients.
This study aimed to identify the incidence of and risk factors for pancreatic cancer in chronic pancreatitis patients.
Chronic ...pancreatitis patients admitted to our center from January 2000 to December 2013 were enrolled. Cumulative rates of pancreatic cancer and survival rates were calculated. The standardized incidence ratio was calculated based on the pancreatic cancer incidence in general population of China. Risk factors for pancreatic cancer were identified.
In a total of 1656 patients, the median follow-up duration was 8.0 years. Pancreatic cancer was detected in 21 patients (1.3%). The expected number of cases of pancreatic cancer was 1.039, yielding a standardized incidence ratio of 20.22. The standardized incidence ratios for patients with a >60 pack-year smoking history were much higher (145.82). Two risk factors for pancreatic cancer were identified: age at the onset of chronic pancreatitis (hazard ratio, 1.05) and a >60 pack-year smoking history (hazard ratio, 11.83).
The risk of pancreatic cancer is markedly increased in chronic pancreatitis patients compared with the general population, especially in patients with an older age at onset and a >60 pack-year smoking history. The high-risk populations were suggested to be followed up closely.
Abstract The emerging multi-channel GaN architecture offers great opportunities in high-performance devices, whose performance is fundamentally determined by the density and distribution of electrons ...among the parallel multi-channels. In this work, we present approaches to design multi-channel GaN heterostructures based on a proposed physical-based model and experimental results. The model presents excellent accuracy based on comparisons with TCAD and experimental results. Impacts of key designing parameters upon the two-dimensional electron gas density and the distribution of electrons were systematically investigated within undoped and doped multi-channels, presenting a criterion to determine the maximum channel thickness and evaluate the multi-channel design, and offering a design guideline to design and optimize a multi-channel-GaN heterostructure for a given targeted application.
Background and Aim
Pancreatic extracorporeal shock wave lithotripsy (P‐ESWL) is a first‐line treatment for chronic pancreatitis (CP) patients with pancreatic stones. However, the performance of ...P‐EWSL in geriatric patients remains unclear. We aimed to evaluate the safety and efficacy of P‐ESWL for them.
Methods
This prospective study was conducted in painful CP patients who underwent P‐ESWL. Patients aged over 65 years were included in geriatric group; patients aged under 65 years were assigned to control group. For the long‐term follow‐up investigation, geriatric patients were matched with patients from the control group in a 1:1 ratio. Primary outcomes were complications of P‐ESWL and pain relief. Secondary outcomes included stone clearance, physical and mental health, quality of life score, changes in exocrine and endocrine pancreatic function, and survival.
Results
From March 2011 to March 2016, P‐ESWL was performed in 1404 patients (72 in the geriatric group and 1332 in the control group). No significant differences were observed in complications of P‐ESWL between the two groups (P = 0.364). Among the 67 (67/72, 93.1%) geriatric patients who underwent follow up for 4.02 years, complete pain relief was achieved in 53 patients, which was not significantly different from that of matched controls (54/70; P = 0.920). The death in the geriatrics was significantly higher (P = 0.007), but none of them were correlated with P‐ESWL.
Conclusions
P‐ESWL is safe and effective for geriatric CP patients with pancreatic stones. It can promote significant pain relief and stone clearance and improve quality of life and mental and physical health.
In this study, 7075 aluminum matrix composites reinforced with 1.5 wt.% nanosized Al2O3 were fabricated by ultrasonic vibration. The effect of T6 heat treatment on both microstructure and hardness of ...nanosized Al2O3 reinforced 7075 (Al2O3np/7075) composites were studied via scanning electron microscopy, energy dispersive X-ray spectrometry, X-ray diffraction, transmission electron microscopy, and hardness tests. The Mg(Zn,Cu,Al)2 phases gradually dissolved into the matrix under solution treatment at 480 °C for 5 h. However, the morphology and size of Al7Cu2Fe phases remained unchanged due to their high melting points. Furthermore, the slenderness strips MgZn2 phases precipitated under aging treatment at 120 °C for 24 h. Compared to as-cast composites, the hardness of the sample under T6 heat treatment was increased ~52%. The strengthening mechanisms underlying the achieved hardness of composites are revealed.
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