An analysis was conducted on marker genes indicating the induction of clear cell renal cell carcinoma (ccRCC) and their role in the pathogenesis of this oncological disease. The diagnostic and ...therapeutic approaches for ccRCC are evolving with the identification of marker genes. The use of molecular biology methods has enabled the detection of somatic mutations in RCC marker genes like VHL, BAP1, OGG1, FLCN, MDM2, TP53, and RNF139. Targeted drugs like sorafenib, sunitinib, bevacizumab, pazopanib, axitinib, everolimus, and temsirolimus are being utilized in the treatment of RCC metastases. The diagnostic value of specific alleles in marker genes like rs1642742 and rs779805 of the VHL gene, rs1052133 of the OGG1 gene, rs2279744 of the MDM2 gene, and rs1597368777 of the TP53 gene has been confirmed in detecting ccRCC. Mutations in these marker genes play a significant role in the pathogenesis of ccRCC and are associated with the development and progression of the disease. The identification of these mutations can aid in the diagnosis, prognosis, and treatment of ccRCC. Marker genes are essential in understanding the molecular mechanisms underlying ccRCC. Mutations in genes like VHL, BAP1, OGG1, FLCN, MDM2, TP53, and RNF139 are key drivers of ccRCC pathogenesis. The diagnostic and therapeutic implications of these mutations are significant in the management of ccRCC. Further research into the role of marker genes in ccRCC is essential for developing targeted therapies and improving patient outcomes.
In order to clarify the peculiarities of the osmotic concentration of urine in the dynamics of the development of alloxan-induced experimental diabetes mellitus (DDM), studies were conducted on 54 ...sexually mature non-linear male white rats, in which DDM was simulated by a single intraperitoneal injection of alloxan solution (Alloxan monohydrate, "Acros Organics", Belgium) at a dose of 160 mg/kg of body weight after a previous 12-hour food deprivation with preserved access to water. 10, 25, and 45 days after the introduction of the diabetogenic substance, 24 alloxan-diabetic rats, as well as 30 control (intact) animals, were loading with tap water in a volume of 5% of body weight, urine was collected for 2 hours, euthanasia was performed by decapitation under light ether anesthesia. In blood samples, the level of glucose and creatinine was determined, in urine samples, after evaluating the water-induced 2-hour diuresis (in ml/100 g of body weight in 2 hours), the concentration of creatinine was determined, the clearance of endogenous creatinine, the clearance of osmotically free water, as well as relative (tubular) reabsorption of water, determined the content of glucose, urea, its excretion, including per 100 μl of glomerular filtrate, as well as urine osmolarity.
The results of the study allow us to state that as the duration of experimental diabetes is prolonged, the pathogenetic significance of hyperfiltration, which determines the speed of fluid movement along the tubule and the intensity of its absorption, weakens in relation to the processes of osmotic concentration of urine, yielding to other factors, such as the osmolarity of the medulla of the kidneys, as well as the presence of substances in the liquid of the distal segment and collecting tubes, the reabsorption of which can change their concentration in the collecting tubes as the urine progresses. In diabetes, in addition to glucose, such substances include urea. Attempts to analyze the renal excretion of urea revealed that changes in its urinary concentration are definitely correlated with the dynamics of changes in urine osmolality. Moreover, it can be assumed that urea transport disorders in the diabetic kidney occur earlier than the ability to excrete osmotically active substances in general. A joint study of the processes of renal transport of urea and urine osmolarity can serve as an early verifier of tubulointerstitial damage. Nevertheless, to increase the reliability of the interpretation of the features of the osmotic concentration of urine in diabetes, we consider it necessary to study the nature of the renal transport of electrolytes (sodium, potassium, calcium, and ammonium).
Background. Today, acute pancreatitis is a common form of acute abdomen in the clinic, the incidence of which has been increasing in recent years. The aim of the work was to study the dynamics of ...ultrastructural changes of the hemocapillaries of the alveolar wall of the lungs in the late stages of experimental acute pancreatitis. Material and methods. The experiments were carried out on 54 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine at a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. The ultrastructural analysis showed that already 3 days after the study, dystrophic-destructive changes, as well as adhesion and aggregation of leukocytes, were detected in the endothelial cells. As the study period increased (5–7 days), the intensity of changes in the hemocapillaries of the alveolar wall increased significantly. In the lumen erythrocyte sludge, thromboleukocyte adhesion and aggregation are determined in hemocapillaries. Conclusion. Acute experimental pancreatitis is accompanied by marked changes in the ultrastructural structure of hemocapillaries of the alveolar wall. The nature and severity of structural changes in the hemocapillaries of the alveolar wall depends on the duration of the course of arginine-induced acute pancreatitis.
Introduction and aim. We previously found that diabetic angiopathy is accompanied by an increase in the level of desquamated endothelial cells (CECs) circulating in the blood, commensurate with its ...severity. In this study, we set ourselves the aim of analyzing the level of CECs in patients with cardiovascular pathology and their relationship with the level of blood pressure and routine clinical indicators. Material and methods. The object of clinical observation were patients with stage II hypertension, who were receiving outpatient treatment. Among them, 23 men (62.3±13.2 ys) and 39 women (65.1±10.6 ys). Concomitant ischemic heart disease was diagnosed in 16 men (70±10%) and 28 women (72±7%). The main subject of the study was blood pressure and the level of CECs. In addition, routine general blood analysis and determined metabolic parameters were performed. Results. Using the method of cluster analysis, the sample was divided into 4 homogeneous groups, different from each other. It was found that in 18 patients of the first cluster, the youngest in the sample, the minimum level of hypertension for the sample occurs against the background of unchanged CECs levels and other registered parameters, instead, it is accompanied by moderate increased creatininemia and a significantly increased rate of erythrocyte sedimentation and a reduced level of hemoglobin and color index. In 18 patients of the second cluster, moderately elevated levels of terminally and markedly changed CECs were found in combination with minimally expressed signs of hyperchromic anemia. In the third cluster (n=16), the levels of terminally and markedly changed CECs are significantly increased in combination with a reduced level of urea and LP index against the background of moderate hyperchromic anemia. Finally, in 10 patients of the fourth cluster, the oldest in the sample, with maximally expressed hypertension, it is accompanied by maximally elevated levels of all three types of СEСs against the background of moderate hyperchromic anemia. Conclusion. Desquamated plasma endothelial cells can be one of the criteria for the severity of hypertonic disease.
Background. With this article, we start the project "Functional relationships between the parameters of acupuncture points and the neuro-endocrine-immune network", thereby joining the construction of ...a bridge between the Western and Eastern paradigms of medicine. Materials and methods. The object of observation were 10 women (32-76 years) and 10 men (37-67 years) examined twice with a weekly interval. The volunteers were considered practically healthy, but the initial testing revealed deviations from the norm in a number of parameters of the neuro-endocrine-immune (NEI) network as a manifestation of maladaptation. We recorded electrical conductivity in acupuncture points (AP) Pg(ND), TR(X) and MC(AVL) as well as parameters of NEI network, gas discharge visualization (GDV) and metabolism. Results. The preliminary analysis of the parameters revealed in the female SO drastic deviations from both the reference and the average for the sample, the levels of electrical conductivity of three pairs of AP - that is, the so-called "falling values", which are usually removed from the subsequent analysis as artifacts. However, we found the same drastic or significant deviations of a number of other parameters of GDV, EEG, HRV, adaptation hormones, immunity and metabolism in this patient. Therefore, the registered drastic decrease in electrical conductivity of AP is by no means an artifact, but reflects the peculiarities of the NEI network, GDV and metabolism of the patient's body during the first examination. It is significant that upon re-examination, the deviations of the parameters significantly or completely approached the range of the average for the sample ± SD or the norm ± SD. Conclusion. The above gives us a reason to initiate a broad discussion of the problem of "falling variables" as carriers of unique information that is ignored and lost.
Background. This publication initiates the project "Neuro-endocrine regulation of the clearance of nitrogenous metabolites". The first stage of project implementation is the clarification of ...peculiarities of metabolic and neuro-endocrine accompaniments of urate-losing and urate-retaining kidneys. Materials and Methods. The object of observations were patients with chronic pyelonephritis in the phase of remission (34 males aged 23-70 years and 10 females aged 33-76 years). Testing was performed twice - on admission and after 7-10 days of standard balneotherapy on Truskavets Spa. The battery of tests was created in line with concepts functional-metabolic continuum and NEI network. The content of nitrogenous metabolites and electrolytes was determined in daily urine and blood serum. In addition, indicators of the lipoprotein spectrum and lipids peroxidation, as well as the level of the main adaptation hormones, were determined in the blood. The state of the autonomic nervous system was assessed by the HRV method. Results. In 63.6% of cases, the renal clearance of uric acid was within the classical norm (±2σ), in 12.5% it was moderately reduced, but in the remaining cases it was increased, including drastically in 3 cases. The method of discriminant analysis revealed 20 parameters, according to the totality of which all 4 clusters of uric acid clearance clearly differ from each other (classification accuracy 100%). Conclusion. Constitutionally distinct types of uric acid metabolism (euricosis, urate-losing and urate-retaining kidneys) are characterized by specific constellations of metabolic and autonomic-endocrine parameters.
Background. Today, acute pancreatitis is one of the most common and severe visceral disorders in patients worldwide. The aim of the work was to study the dynamics of ultrastructural changes of type ...II alveolocytes in the long-term periods of development of experimental acute pancreatitis. Material and methods. The experiments were carried out on 54 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine at a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. It was found that three days after the start of the study, along with dystrophic-destructive changes, type II alveolocytes in a state of increased functional activity were observed. Continuation of the experiment (5 days) leads to the progression of ultrastructural changes in type II alveolocytes are determined, which are most pronounced on the 7 day of the study. Conclusion. Acute experimental pancreatitis is accompanied by marked changes in the ultrastructural structure of type II alveolocytes. The nature and severity of structural changes of type II alveolocytes depends on the duration of the course of arginine-induced acute pancreatitis.
Introduction: Liver cirrhosis is a disease whose development is influenced by many factors, including those related to lifestyle. The disease causes disturbances in the functioning of not only the ...liver but also many other organs and systems. During the course of the disease, there are a number of complications that affect the patient's functioning in all areas. Patients with liver cirrhosis require constant and systematic treatment and comprehensive care, including care provided by nursing staff. Purpose of research: Analysis of problems occurring in the care of patients with liver cirrhosis Material and methods: The study included 103 patients with liver cirrhosis, hospitalized from October 10, 2022 to April 10, 2023. in the Observation and Infection Ward for Adults of the SPZOZ in Puławy. The method used in the work was a diagnostic survey, the technique was a survey, and the research tool was an original survey questionnaire developed for the needs of the research. Results: Patients with liver cirrhosis have many problems of a physical nature and related to non-compliance with medical recommendations, which may affect the inhibition of the progression of the disease, the prevention of complications and the improvement of the general condition. Conclusions: Patients with liver cirrhosis have a number of physical problems, the knowledge of which may affect the organization of work of nursing staff, facilitate the identification of problems, help determine the goal of care and plan nursing interventions. Not all respondents followed the specialist's recommendations, including: in the field of pharmacotherapy, dietary recommendations, performing check-ups, reporting for follow-up visits and physical activity. An important element in the care of a patient with liver cirrhosis is patient education and motivation to follow recommendations that are important in stopping the progression of the disease, preventing complications and improving the general condition.
Background. Nowadays, acute pancreatitis continues to remain an actual problem both in surgery and in intensive care medicine. The aim of the work was to study the dynamics of ultrastructural changes ...of alveolar macrophages in the long-term development of experimental acute pancreatitis. Material and methods. The experiments were carried out on 62 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine in a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. It was found that three days after the start of the study, significant heterogeneity of alveolar macrophages was observed in the alveolar lumen. Next to active phagocytic macrophage elements with dystrophic-destructive changes are determined. Continuation of the experiment (5 days) leads to the progression of submicroscopic changes in alveolar macrophages, which are most pronounced on the7 day of the study. Conclusion. Acute experimental pancreatitis is accompanied by pronounced changes in the ultrastructural structure of alveolar macrophages. The features and severity of structural changes in alveolar macrophages depends on the duration of arginine-induced acute pancreatitis.
Background. During the implementation of the project "Physiological activity of uric acid", our group discovered four variants of the combination of levels of uricemia and uricosuria both in healthy ...rats and in people with chronic pyelonephritis and cholecystitis in the remission phase, which are accompanied by characteristic constellations of parameters of the autonomic nervous, endocrine, and immune systems, as well as the exchange of nitrogenous metabolites and electrolytes, the levels of which correlate with uricemia and/or uricosuria. The aim of this study is to clarify the relationship between parameters of uric acid metabolism and electroencephalogram (EEG) in humans. Materials and methods. Under an observations were 34 males and 10 females by age 24-76 years with chronic pyelonephritis and cholecystitis in the phase of remission. The object of the study was serum and urine levels of uric acid (uricase method) as well as EEG. Results. It was identified 12 parameters as characteristic of uric acid metabolism clusters. Another 42 EEG parameters were found to be out of the discriminant model, despite the clear recognition ability. A strong canonical correlation between EEG parameters and uricemia (R=0,729) and a moderate correlation with uricosuria (R=0,553) were revealed. Even stronger connections were found between changes in parameters of uric acid metabolism and EEG under the influence of balneotherapy: R=0,901 and 0,681 respectively. Conclusion. Uric acid has both activating and inhibitory effects on EEG parameters. The neurotropic effect of uric acid as a structural analogue of methylxanthines and adenosine is realized, apparently, through various adenosine receptors.
