The demand for animal protein for human consumption has been risen exponentially. Modern animal production practices are associated with the regular use of antibiotics, potentially increasing the ...emerging multi-resistant bacteria, which may have a negative impact on public health. In poultry production, substances capable of maximizing the animals' performance and displaying an antimicrobial activity against pathogens are very well desirable features. Probiotic can be an efficient solution for such a task. In the present work, lactic acid bacteria (LAB) were isolated from chicken cecum and screened for their antagonistic effect towards many pathogens. Their capacity of producing the B-complex vitamins folate and riboflavin were also evaluated. From 314 isolates, three (C43, C175 and C195) produced Bacteriocin-Like Inhibitory Substances (BLIS) against Staphylococcus aureus (inhibition zones of 18.9, 21.5, 19.5 mm, respectively) and also inhibited the growth of Salmonella Heidelberg. The isolate C43 was identified as Enterococcus faecium, while C173 and C195 were both identified as Lactococcus lactis subsp. lactis. Moreover, the isolates L. lactis subsp. lactis strains C173 and C195 demonstrated high potential to be used as probiotic in poultry feed, in addition to their advantage of producing folate (58.0 and 595.5 ng/mL, respectively) and riboflavin (223.3 and 175.0 ng/mL, respectively).
Honey is an excellent alternative to be used in different applications by the food, pharmaceutical, and cosmetics industries. However, the use of honey in its liquid form can present several ...disadvantages due to its high viscosity and density. In addition, honey can naturally crystallize during storage time, limiting its application in new products by industries. Thus, an alternative to using liquid honey in industry is the powder form. The objective of this study was to obtain honey powder by spray drying using a plant-based protein as a carrier. The honey used as raw material showed acceptable physicochemical parameters by the legislation of honey quality control, such as pH of (4.0 ± 0.1), the water content of (20.70 ± 0.3) g/100 g, ash of (0.48 ± 0.02) g/100 g, the fructose content of (35.42 ± 0.14) g/100 g, (43.05 ± 0.46) g/100 g for glucose, (0.56 ± 0.02) g/100 g for sucrose, and 5-hydroxymethylfurfural (HMF) value of (37.47 ± 1.04) mg/kg of honey, leading to the production of the honey powder. The mixture of fresh honey and plant-based protein was made with dispersions of 15, 16 and 18 g/100 g of honey using isolated rice protein as a carrier (total solid at 30 g/100 g d.b.). Finally, the honey powder produced was physicochemically characterized. The dispersion with 16 g/100 g of honey achieved an intermediary sugar/honey recovery of 35.93 ± 0.82% and 68.54 ± 2.16% of total protein recovery, and the spray drying yield was satisfactory > 50%. The overall results suggested that this dispersion is the ideal formulation when commercialization of the honey powder as an ingredient is intended.
Celotno besedilo
Dostopno za:
BFBNIB, DOBA, GIS, IJS, KISLJ, NUK, PNG, UILJ, UKNU, UL, UM, UPUK
Bee pollen has been promoted as a dietary supplement for humans due to its nutritional and bioactive properties. Sixty-two samples of Apis mellifera dehydrated bee pollen collected in Brazil (eight ...states and Federal District) were analyzed for phenolic compounds, flavonoids, antioxidant activity using DPPH, β-carotene and ORAC methods, and antimicrobial activity. The values obtained for total phenolic compounds ranged from 12.60 to 84.22 mg GAE/g bee pollen while total flavonoids oscillated between 1.90 and 36.85 mg quercetin/g bee pollen. The IC
50
ranged from 0.35 to 13.42 mg bee pollen/mL of extract. The inhibition percentages ranged from 52.58 to 98.37% of bee pollen extract using the β-carotene method. When measured by the ORAC method, antioxidant activity was between 132.98 and 575.85 μmols eq. Trolox/g bee pollen. Bee pollen efficiently inhibited the growth of all the microorganisms studied. Candida albicans was the most resistant, while Staphylococcus epidermides was the most sensitive.
Bee pollen (BP) has been increasingly studied because it contains a wide variety of bioactive compounds, including vitamins. Brazilian botanical diversity, together with the potential of local ...beekeeping production, makes BP's capacity as a food source of bioactive compounds major focus for research. In this scenario, the objective of this study was to screen and evaluate the chemical composition, including antioxidant vitamins, of BP samples from southern Brazil, and to correlate them with their botanical origin. Analyses of nutritional composition were performed to compare them with the quality parameters established by Brazilian and international regulations. Additionally, individual sugars and vitamins (C, E and pro-vitamin A) were quantified and microscopic analysis for taxon classification was performed to correlate with vitamins and nutritional composition. The results of the chemical analysis showed that the samples were in accordance with the relevant regulations. The composition of vitamins and pollen types varied among the samples. Some BP could be classified as a source of a particular vitamin in a standard dose (25g). Lipid and protein content from Rio Grande do Sul presented higher mean values (p<0.05) compared with the other two states. Some correlations between chemical composition and botanical taxon were observed. Principal component analysis showed that the samples from the states of Rio Grande do Sul and Paraná presented similarities in terms of composition for each location. HCA and PLSDA were not able to classify the samples based on the chemical markers used. The analysis of vitamins confirmed that BP from this region can be a good source of antioxidant vitamins and that it can provide important nutritional information to food researchers and bioactive compounds for consumers.
•Investigation of bioactive compounds on bee pollen from southern Brazil is proposed.•We evaluate the nutritional composition and antioxidant vitamins of bee pollen.•Pollen analysis permits correlation with some bioactive compounds.•Multivariate techniques explain chemical patterns according to producing region.
Sixty-two commercial samples of dehydrated bee pollen (Apis mellifera bees) collected in Brazil (eight states and the Federal District) were analyzed for commercial quality (moisture, ash, lipids, ...proteins, and carbohydrates), and hygiene (aerobic mesophiles, yeasts and molds, coliforms, Escherichia coli, Staphylococcus aureus and sulfite-reducing Clostridium). The analyzed samples were within the regulatory limits established by the legislation for ash, lipid and protein, except for moisture content. The analyzed hygiene parameters evaluated for bee pollen are not regulated by the Brazilian legislation, and the data discussed can contribute to regulatory agencies. Only moderate and weak correlations were observed between dehydrated pollen samples and the parameters evaluated.
Abstract With worldwide recognition of the açaí berry as a source of nutrients and promising raw material, its residues/co-products such as peels and seeds have become an environmental problem. The ...present work aimed to characterize the pulp residue (peel + pulp), fresh dreg, and respective flour, as well as the seed and respective flour. The fractions were analyzed for physical, chemical, technological parameters, antinutritional compounds, and antioxidant profiles. The results showed that the peel + pulp is a source of lipids, soluble and insoluble fiber, potassium, calcium, magnesium, and antioxidants. The fresh dreg is a source of insoluble fiber; dreg flour is a source of carbohydrates and insoluble fiber; the seed and its respective flour are sources of carbohydrates, insoluble and soluble fiber, contain phytic acid, condensed tannins, and antioxidants. Such results demonstrate the possibility of incorporating these co-products in food formulations, besides enabling an efficient destination for these agribusiness residues.
SUMMARY
As sessile organisms, plants must adapt their physiology and developmental processes to cope with challenging environmental circumstances, such as the ongoing elevation in atmospheric carbon ...dioxide (CO2) levels. Nicotinamide adenine dinucleotide (NAD+) is a cornerstone of plant metabolism and plays an essential role in redox homeostasis. Given that plants impaired in NAD metabolism and transport often display growth defects, low seed production and disturbed stomatal development/movement, we hypothesized that subcellular NAD distribution could be a candidate for plants to exploit the effects of CO2 fertilization. We report that an efficient subcellular NAD+ distribution is required for the fecundity‐promoting effects of elevated CO2 levels. Plants with reduced expression of either mitochondrial (NDT1 or NDT2) or peroxisomal (PXN) NAD+ transporter genes grown under elevated CO2 exhibited reduced total leaf area compared with the wild‐type while PXN mutants also displayed reduced leaf number. NDT2 and PXN lines grown under elevated CO2 conditions displayed reduced rosette dry weight and lower photosynthetic rates coupled with reduced stomatal conductance. Interestingly, high CO2 doubled seed production and seed weight in the wild‐type, whereas the mutants were less responsive to increases in CO2 levels during reproduction, producing far fewer seeds than the wild‐type under both CO2 conditions. These data highlight the importance of mitochondrial and peroxisomal NAD+ uptake mediated by distinct NAD transporter proteins to modulate photosynthesis and seed production under high CO2 levels.
Significance Statement
The beneficial effect of elevated atmospheric CO2 on photosynthesis and seed yield requires correct intracellular NAD+ transport.
Vitamin K: content in food consumed in São Paulo, Brazil Faria, Simone Aparecida dos Santos Conceição; Arruda, Vanilda Aparecida Soares de; Araújo, Elias da Silva ...
Brazilian Journal of Pharmaceutical Sciences,
01/2017, Letnik:
53, Številka:
2
Journal Article
Recenzirano
Odprti dostop
Recent research on Vitamin K has shown its importance in maintaining vascular and bone health. Brazilian food composition tables do not show phylloquinone content in national foods. These data are ...needed to obtain more reliable results in nutritional status assessment studies of individuals in relation to this vitamin as studies have shown a geographical influence in food phylloquinone content. This study aims to determine phylloquinone (Vitamin K1) levels in its most important source: dark green leaved vegetables. Several varieties of vegetables were purchased directly from CEAGESP (General Warehouse Company of São Paulo) at different times. Phylloquinone was extracted using organic solvents and quantified by High Performance Liquid Chromatography - HPLC. Results show the concentrations of phylloquinone in commonly consumed foodstuffs. In general, results showed variations with data from literature on the amount of Vitamin K in the plants analysed.
As sessile organisms, plants must adapt their physiology and developmental processes to cope with challenging environmental circumstances, such as the ongoing elevation in atmospheric carbon dioxide ...(CO
) levels. Nicotinamide adenine dinucleotide (NAD
) is a cornerstone of plant metabolism and plays an essential role in redox homeostasis. Given that plants impaired in NAD metabolism and transport often display growth defects, low seed production and disturbed stomatal development/movement, we hypothesized that subcellular NAD distribution could be a candidate for plants to exploit the effects of CO
fertilization. We report that an efficient subcellular NAD
distribution is required for the fecundity-promoting effects of elevated CO
levels. Plants with reduced expression of either mitochondrial (NDT1 or NDT2) or peroxisomal (PXN) NAD
transporter genes grown under elevated CO
exhibited reduced total leaf area compared to the wild-type while PXN mutants also displayed reduced leaf number. NDT2 and PXN lines grown under elevated CO
conditions displayed reduced rosette dry weight and lower photosynthetic rates coupled with reduced stomatal conductance. Interestingly, high CO
doubled seed production and seed weight in the wild-type, whereas the mutants were less responsive to increases in CO
levels during reproduction, producing far fewer seeds than the wild-type under both CO
conditions. These data highlight the importance of mitochondrial and peroxisomal NAD
uptake mediated by distinct NAD transporter proteins to modulate photosynthesis and seed production under high CO
levels.
Changes in eating patterns have been leading to an increase in the consumption of ultra-processed foods (UPF), negatively impacting the quality of the diet and generating risk of harm to the health ...of the adult population, however, there is no systematized evidence of the impact of UPF in maternal-child health. Thus, in this study we aimed to evaluated the association between UPF consumption and health outcomes in the maternal-child population.
Systematic review registered on the International Prospective Register of Systematic Reviews (PROSPERO) (CRD42021236633), conducted according to the PRISMA diagram in the following databases: PubMed, Medline, Scopus, Web of Science, Scielo, and CAPES thesis and dissertation directory. We included original cross-sectional, case-control and cohort studies in any language. Eligibility criteria were (a) food consumption assessment by the NOVA classification, (b) health outcome (nutritional or diseases), and (c) maternal-child population (pregnant, lactating women and infants/children). All data were analyzed and extracted to a spreadsheet structured by two independent reviewers. We evaluated the methodological quality of the studies included using the Newcastle-Otawa Scale and RoB 2.
Searches retrieved 7,801 studies and 15 contemplated the eligibility criteria. Most studies included were cohort studies (
= 8, 53%), had children as their population (
= 9, 60%) and only one study evaluated UPF consumption in infants and lactating women. Panoramically, we observed that a higher participation of UPF in children's diet has been associated with different maternal-child outcomes, such as increase of weight gain, adiposity measures, overweight, early weaning, lower diet quality, metabolic alterations, diseases, and consumption of plastic originated from packaging. Only one of the studies included did not present high methodological quality.
Despite the limited literature on UPF consumption and health outcomes in the maternal-child population, the highest UPF consumption negatively impacted nutrition and disease development indicators in pregnant, lactating women and children. Considering the expressive participation of these foods in the diet, other studies should be conducted to further investigate the impact of UPF consumption on different health indicators, especially in the lactation phase for this was the one to present the most important knowledge gap.
https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021236633, identifier CRD42021236633.