The integration of first‐ (1G) and second‐generation (2G) ethanol production by adding sugarcane juice or molasses to lignocellulosic hydrolysates offers the possibility to overcome the problem of ...inhibitors (acetic acid, furfural, hydroxymethylfurfural and phenolic compounds), and add nutrients (such as salts, sugars and nitrogen sources) to the fermentation medium, allowing the production of higher ethanol titers. In this work, an 1G2G production process was developed with hemicellulosic hydrolysate (HH) from a diluted sulfuric acid pretreatment of sugarcane bagasse and sugarcane molasses. The industrial Saccharomyces cerevisiae CAT‐1 was genetically modified for xylose consumption and used for co‐fermentation of sucrose, fructose, glucose, and xylose. The fed‐batch fermentation with high cell density that mimics an industrial fermentation was performed at bench scale fermenter, achieved high volumetric ethanol productivity of 1.59 g L−1 h−1, 0.39 g g−1 of ethanol yield, and 44.5 g L−1 ethanol titer, and shown that the yeast was able to consume all the sugars present in must simultaneously. With the results, it was possible to establish a mass balance for the global process: from pretreatment to the co‐fermentation of molasses and HH, and it was possible to establish an effective integrated process (1G2G) with sugarcane molasses and HH co‐fermentation employing a recombinant yeast.
Background
The root system plays a major role in plant growth and development and root system architecture is reported to be the main trait related to plant adaptation to drought. However, ...phenotyping root systems in situ is not suited to high-throughput methods, leading to the development of non-destructive methods for evaluations in more or less controlled root environments. This study used a root phenotyping platform with a panel of 20 japonica rice accessions in order to: (i) assess their genetic diversity for a set of structural and morphological root traits and classify the different types; (ii) analyze the plastic response of their root system to a water deficit at reproductive phase and (iii) explore the ability of the platform for high-throughput phenotyping of root structure and morphology.
Results
High variability for the studied root traits was found in the reduced set of accessions. Using eight selected traits under irrigated conditions, five root clusters were found that differed in root thickness, branching index and the pattern of fine and thick root distribution along the profile. When water deficit occurred at reproductive phase, some accessions significantly reduced root growth compared to the irrigated treatment, while others stimulated it. It was found that root cluster, as defined under irrigated conditions, could not predict the plastic response of roots under drought.
Conclusions
This study revealed the possibility of reconstructing the structure of root systems from scanned images. It was thus possible to significantly class root systems according to simple structural traits, opening up the way for using such a platform for medium to high-throughput phenotyping. The study also highlighted the uncoupling between root structures under non-limiting water conditions and their response to drought.
Rice is more vulnerable to drought than maize, wheat, and sorghum because its water requirements remain high throughout the rice life cycle. The effects of drought vary depending on the timing, ...intensity, and duration of the events, as well as on the rice genotype and developmental stage. It can affect all levels of organization, from genes to the cells, tissues, and/or organs. In this study, a moderate water deficit was applied to two contrasting rice genotypes, IAC 25 and CIRAD 409, during their reproductive stage. Multi‐level transcriptomic, metabolomic, physiological, and morphological analyses were performed to investigate the complex traits involved in their response to drought. Weighted gene network correlation analysis was used to identify the specific molecular mechanisms regulated by each genotype, and the correlations between gene networks and phenotypic traits. A holistic analysis of all the data provided a deeper understanding of the specific mechanisms regulated by each genotype, and enabled the identification of gene markers. Under non‐limiting water conditions, CIRAD 409 had a denser shoot, but shoot growth was slower despite better photosynthetic performance. Under water deficit, CIRAD 409 was weakly affected regardless of the plant level analyzed. In contrast, IAC 25 had reduced growth and reproductive development. It regulated transcriptomic and metabolic activities at a high level, and activated a complex gene regulatory network involved in growth‐limiting processes. By comparing two contrasting genotypes, the present study identified the regulation of some fundamental processes and gene markers, that drive rice development, and influence its response to water deficit, in particular, the importance of the biosynthetic and regulatory pathways for cell wall metabolism. These key processes determine the biological and mechanical properties of the cell wall and thus influence plant development, organ expansion, and turgor maintenance under water deficit. Our results also question the genericity of the antagonism between morphogenesis and organogenesis observed in the two genotypes.
Two rice genotypes were subjected to a moderate water deficit applied during the reproductive phase. Multi‐level transcriptomic, metabolomic, physiological, and morphological analyses were performed to investigate the complex traits involved in their response to drought. A holistic analysis of these data allowed us to identify, for each genotype, underlying mechanisms participating in growth regulation under water stress.
Biomass alkaline deacetylation prior to acid pretreatment can be a promising alternative to reduce the toxicity of hemicellulosic hydrolysates and improve second-generation bioethanol production. In ...this paper, the effect of alkaline deacetylation of sugarcane bagasse on bioethanol production by
Spathaspora passalidarum
was evaluated. Sugarcane bagasse deacetylated hemicellulosic hydrolysate (DHH) was processed using the following sequence: (1) deacetylation (0.4%, w/v NaOH, 70 °C, 3 h) and (2) acid pretreatment (0.5% (v/v) of H
2
SO
4
, 140 °C, 15 min). Non-deacetylated, hemicellulosic hydrolysate (HH), was obtained applying only acid pretreatment (0.5% (v/v) of H
2
SO
4
, 140 °C, 15 min). Biomass deacetylation reduced the content of acetic acid and some phenolic compounds in the hydrolysate (DHH) compared to acid pretreatment (HH), which resulted in its low toxicity. Thus, the bioethanol production with DHH was of 16.92 g L
−1
, whereas only 1.3 g L
−1
of bioethanol was obtained with HH fermentation. Deacetylation process provided a 13-fold increase in bioethanol production by
S. passalidarum
, showing that alkaline deacetylation followed by sulfuric acid pretreatment is a promising strategy to increase bioethanol production. This procedure provided a simple and practical alternative to the classic methods of detoxification of hemicellulosic hydrolysate from sugarcane bagasse.
Background: Nutritional knowledge is one of the factors that can lead to adopting healthy eating habits and, consequently, favoring sports performance. Aim: The study aimed to assess the nutritional ...knowledge and its subsections general and sports nutritional knowledge of recreational athletes. Methods: A validated, translated, and adapted 35-item questionnaire was used to assess total (TNK), general—GNK (11 questions), and sports—SNK (24 questions) nutritional knowledge. The Abridged Nutrition for Sport Knowledge Questionnaire (ANSKQ) was provided online using Google Forms. Four hundred and nine recreational athletes (male: 173, female: 236, age = 32.4 ± 9.6 years) completed the questionnaire. Results: The mean TNK (50.7%) and GNK (62.7%) scores were classified as “average” and higher than SNK (45.2%), which was classified as “poor.” Male participants had SNK and TNK scores higher than females, but not for GNK. The youngest participants (18–24 years) had TNK, SNK, and GNK scores higher than other age groups (p < 0.05). Participants who reported previous nutritional appointments with a nutritionist had higher TNK, SNK, and GNK scores than those without it (p < 0.05). Those with “advanced” formal nutrition education (university students, graduate, or postgraduate in Nutrition) scored higher than those of groups “none” and “intermediate,” for TNK (advanced = 69.9%, intermediate = 52.9%, and none = 45.0%, p < 0.0001), GNK (advanced = 74.7%, intermediate = 63.8%, and none = 59.2%, p < 0.0001), and SNK, (advanced = 67.5%, intermediate = 48.0%, and none = 38.5%, p < 0.0001). Conclusion: Results suggest a lack of nutritional knowledge in recreational athletes, mainly those without an appointment with a registered nutritionist and formal nutritional education.
Herbal mate (Ilex paraguariensis A.St.-Hil.) leaves are traditionally used for their stimulant, antioxidant, antimicrobial, and diuretic activity, presenting as principal components polyphenolic ...compounds. The aim of this work was to develop an innovative, non-dairy, functional, probiotic, fermented beverage using herbal mate extract as a natural ingredient which would also be hypocholesterolemic and hepatoprotective. Among different strains used, Lactobacillus acidophilus was selected as the best for fermentation. The addition of honey positively affected the development of L. acidophilus and the formulated beverage maintained microbial stability during shelf life. Key ingredients in the extract included xanthines, polyphenols and other antioxidants with potential health benefits for the consumer. Caffeine levels and antioxidant activity were also studied. Acceptable levels of caffeine and large antioxidant capacity were observed for the formulation when compared to other antioxidant beverages. An advantage of this product is the compliance to organic claims, while providing caffeine, other phyto-stimulants and antioxidant compounds without the addition of synthetic components or preservatives in the formulation. Sensorial analysis demonstrated that the beverage had good consumer acceptance in comparison to two other similar commercial beverages. Therefore, this beverage could be used as a new, non-dairy vehicle for probiotic consumption, especially by vegetarians and lactose intolerant consumers. It is expected that such a product will have good market potential in an era of functional foods.
Piper aleyreanum is a small tree that is widely distributed in tropical and subtropical regions, mostly in North and South America, and is used as an immunomodulator, analgesic and antidepressant in ...folk medicine.
This study was designed to investigate the antinociceptive, anti-inflammatory and gastric antiulcer activities of the essential oils from the aerial parts of Piper aleyreanum (EOPa) in rodents.
The antinociceptive and anti-inflammatory effects of orally administered EOPa were evaluated in mice subjected to the formalin and pleurisy models, respectively. We also pretreated the rats with EOPa before acute ethanol-induced gastric lesions and measured gastric lesion extension and mucus and glutathione (GSH) levels in the gastric mucosa. Finally, we performed a phytochemical analysis of EOPa.
The chemical composition of EOPa was analyzed by gas chromatography and mass spectrometry (GC/MS), which identified 35 compounds, representing 81.7% of total oil compounds. Caryophyllene oxide (11.5%), β-pinene (9%), spathulenol (6.7%), camphene (5.2%), β-elemene (4.7%), myrtenal (4.2%), verbenone (3.3%) and pinocarvone (3.1%) were the major oil constituents. The oral administration of EOPa (10–1000mg/kg) significantly inhibited the neurogenic and inflammatory phases of formalin-induced licking, with ID50 values of 281.2 and 70.5mg/kg, respectively. The antinociception caused by EOPa (100mg/kg, p.o.) was not reversed by naloxone (1 or 5mg/kg, i.p.) in the formalin test. EOPa (100–300mg/kg, p.o.) did not affect animal motor coordination in an open-field model. In carrageenan-induced pleurisy, EOPa (1–100mg/kg, p.o.) significantly decreased the total cell count, neutrophils and mononuclear cells with mean ID50 values of 53.6, 21.7 and 43.5mg/kg, respectively. In addition, EOPa (1–30mg/kg, p.o.) protected the rats against ethanol-induced gastric lesions with an ID50 value of 1.7mg/kg and increased the mucus and GSH levels of the gastric mucosa to levels similar to those of the non-lesioned group.
These data show for the first time that EOPa has significant antinociceptive and anti-inflammatory actions, which do not appear to be related to the opioid system. EOPa also has interesting gastroprotective effects related to the maintenance of protective factors, such as mucus production and GSH. These results support the widespread use of Piper aleyreanum in popular medicine and demonstrate that this plant has therapeutic potential for the development of phytomedicines with antinociceptive, anti-inflammatory and gastroprotective properties.
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To evaluate the impact of 3 treatment regimens upon health-related quality of life and work productivity using patient-reported outcomes (PROs) in chronic hepatitis C infected patients: sofosbuvir ...(SOF) + daclatasvir (DCV); SOF + DCV + ribavirin (RBV); SOF + simeprevir (SMV).
4 questionnaires were used to evaluate PROs before, during and after treatment: Short Form-36 (SF-36), Chronic Liver Disease Questionnaire (CLDQ) - hepatitis C virus (HCV), Work Productivity and Activity Index, Functional Assessment of Chronic Illness Therapy-Fatigue (FACIT-F).
Of the global sample of 55 patients included in this study; SOF + DCV (
= 10); SOF + DCV + RBV (
= 29); SOF + SMV (
= 16) all had a statistically significant improvement in SF-36, CLDQ and FACIT-F scores during and post-treatment. No statistically significant differences in the PRO questionnaire values were observed between the distinct treatment regimens. The SOF and SMV patient groups presented higher mean PRO variations during and post-treatment, compared to the other groups: SF-36 functional capacity (16.1); SF-36 mental health (21.4); CLDQ activity (1.8); CLDQ emotional function (1.2); FACIT-F physical well-being (8.0); Total FACIT-F (21.6).
Treatment with SOF + DCV, with or without RBV, results in an improved PRO similar to treatment with SOF + SMV in chronic hepatitis C patients.
A goiabeira representa uma importante atividade frutícola no Brasil, com mercado cada vez maior. Porém, desde 1989 vêm sendo relatados severos danos à cultura, causados pelo nematóide Meloidogyne ...enterolobii. Uma das alternativas para solucionar esse problema é a utilização de porta-enxertos com resistência a este patógeno. Este trabalho teve por objetivo a caracterização molecular, com marcadores RAPD, de acessos de Psidium testados quanto à resistência a M. enterolobii e quanto à compatibilidade como porta-enxertos para as goiabeiras comerciais. Foram testados 30 primers, dos quais 19 forneceram resultados nítidos para a amplificação. Foram gerados 163 fragmentos, dos quais 86 polimórficos (63,0%). Em média, cada iniciador produziu 8,6 fragmentos, dos quais 5,4 apresentaram polimorfismo. A análise de agrupamento foi realizada por espécie, os acessos de Psidium sp apresentaram a formação de dois grupos, um formado pelo acesso A-UFLA e o segundo subdividido em quatro subgrupos, sendo os acessos com maiores distâncias genéticas A-Ufla, resistente a M. enterolobii, A-Ufla4 e A-Ufla5, ambos suscetíveis ao nematoide em questão, todos coletados em Lavras-MG, com similaridade aproximada de 66%. Na análise de agrupamento, dos treze acessos de P.cattleyanum, foi possível constatar a formação de dois grandes grupos. Um formado por três acessos suscetíveis a M. enterolobii (A-20.2, A-10.1 e A-9.2) e outro grupo formado por dez acessos. Os acessos se agruparam, conforme a região de origem, em seis grupos, sendo que o mais divergente é originário da região de Lavras - MG, com 0,65 de similaridade, onde as distâncias genéticas variaram de 0,88 a 0,65. Dos treze acessos de P. guineense, todos suscetíveis a M. enterolobii, sendo 12 oriundos de Recife e um de Pelotas (A-14.1) e agruparam-se em dois grupos com similaridades variando de 0,59 a 0,83. Quanto ao estudo de diversidade entre os acessos de goiabeiras, a maior distância genética foi detectada entre o acesso G-Ufla com 0,71 Lavras-MG.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
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