Abstract
Wolf 359 (CN Leo, GJ 406, Gaia DR3 3864972938605115520) is a low-mass star in the fifth-closest neighboring system (2.41 pc). Because of its relative youth and proximity, Wolf 359 offers a ...unique opportunity to study substellar companions around M stars using infrared high-contrast imaging and radial velocity monitoring. We present the results of
Ms
-band (4.67
μ
m) vector vortex coronagraphic imaging using Keck-NIRC2 and add 12 Keck-HIRES and 68 MAROON-X velocities to the radial velocity baseline. Our analysis incorporates these data alongside literature radial velocities from CARMENES, the High Accuracy Radial velocity Planet Searcher, and Keck-HIRES to rule out the existence of a close (
a
< 10 au) stellar or brown dwarf companion and the majority of large gas giant companions. Our survey does not refute or confirm the long-period radial velocity candidate, Wolf 359 b (
P
∼ 2900 days), but rules out the candidate's existence as a large gas giant (>4
M
Jup
) assuming an age of younger than 1 Gyr. We discuss the performance of our high-contrast imaging survey to aid future observers using Keck-NIRC2 in conjunction with the vortex coronagraph in the
Ms
band and conclude by exploring the direct imaging capabilities with JWST to observe Jupiter- and Neptune-mass planets around Wolf 359.
Abstract
The next generation of large ground- and space-based optical telescopes will have segmented primary mirrors. Co-phasing the segments requires a sensitive wavefront sensor capable of ...measuring phase discontinuities. The Zernike wavefront sensor (ZWFS) is a passive wavefront sensor that has been demonstrated to sense segmented-mirror piston, tip, and tilt with picometer precision in laboratory settings. We present the first on-sky results of an adaptive optics fed ZWFS on a segmented aperture telescope, W.M. Keck Observatory's Keck II. Within the Keck Planet Imager and Characterizer light path, the ZWFS mask operates in the
H
band using an InGaAs detector (CRED2). We piston segments of the primary mirror by a known amount and measure the mirror's shape using both the ZWFS and a phase retrieval method on data acquired with the facility infrared imager, NIRC2. In the latter case, we employ slightly defocused NIRC2 images and a modified Gerchberg–Saxton phase retrieval algorithm to estimate the applied wavefront error. We find good agreement when comparing the phase retrieval and ZWFS reconstructions, with average measurements of 408 ± 23 and 394 ± 46 nm, respectively, for three segments pistoned by 400 nm of optical path difference (OPD). Applying various OPDs, we find that we are limited to ∼100 nm OPD of applied piston, due to insufficient averaging of the adaptive optics residuals of our observations. We also present simulations of the ZWFS that help to explain the systematic offset observed in the ZWFS reconstructed data.
Abstract
Maunakea is one of the world’s primary sites for astronomical observing, with multiple telescopes operating over submillimeter to optical wavelengths. With its summit higher than 4200 m ...above sea level, Maunakea is an ideal location for astronomy, with a historically dry, stable climate and minimal turbulence above the summit. Under a changing climate, however, we ask how the (above-)summit conditions may have evolved in recent decades since the site was first selected as an observatory location and how future-proof the site might be to continued change. We use data from a range of sources, including in situ meteorological observations, radiosonde profiles, and numerical reanalyses to construct a climatology at Maunakea over the previous 40 yr. We are interested in both the meteorological conditions (e.g., wind speed and humidity) and the image quality (e.g., seeing). We find that meteorological conditions were, in general, relatively stable over the period with few statistically significant trends and with quasi-cyclical interannual variability in astronomically significant parameters such as temperature and precipitable water vapor. We do, however, find that maximum wind speeds have increased over the past decades, with observed wind speeds above 15 m s
−1
increasing in frequency by 1%–2%, which may have a significant impact on ground-layer turbulence. Further, we note that while the conditions themselves are not necessarily changing significantly, the combination of conditions that lead to dome closures (i.e., freezing conditions, increased summit wind speeds, and/or high humidities) are worsening to the point that the number of closure conditions have more than doubled in the last 20 yr. Importantly, we find that the Fried parameter has not changed in the last 40 yr, suggesting there has not been an increase in optical turbulence strength above the summit. Ultimately, more data and data sources—including profiling instruments—are needed at the site to ensure continued monitoring into the future and to detect changes in the summit climate.
ABSTRACT
The complement system, and specifically C5a, is involved in renal ischemia‐reperfusion (IR) injury. The 2 receptors for complement anaphylatoxin C5a (C5aR1 and C5aR2) are expressed on ...leukocytes as well as on renal epithelium. Extensive evidence shows that C5aR1 inhibition protects kidneys from IR injury; however, the role of C5aR2 in IR injury is less clear as initial studies proposed the hypothesis that C5aR2 functions as a decoy receptor. By Using wild‐type, C5aR1‐/‐, and C5aR2‐/‐ mice in a model of renal IR injury, we found that a deficiency of either of these receptors protected mice from renal IR injury. Surprisingly, C5aR2‐/‐ mice were most protected and had lower creatinine levels and reduced acute tubular necrosis. Next, an in vivo migration study demonstrated that leukocyte chemotaxis was unaffected in C5aR2‐/‐ mice, whereas neutrophil activation was reduced by C5aR2 deficiency. To further investigate the contribution of renal cell‐expressed C5aR2 vs. leukocyte‐expressed C5aR2 to renal IR injury, bone marrow chimeras were created. Our data show that both renal cell‐expressed C5aR2 and leukocyte‐expressed C5aR2 mediate IR‐induced renal dysfunction. These studies reveal the importance of C5aR2 in renal IR injury. They further show that C5aR2 is a functional receptor, rather than a decoy receptor, and may provide a new target for intervention.—Poppelaars, F., van Werkhoven, M. B., Kotimaa, J., Veldhuis, Z. J., Ausema, A., Broeren, S. G. M., Damman, J., Hempel, J. C., Leuvenink, H. G. D., Daha, M. R., van Son, W. J., van Kooten, C., van Os, R. P., Hillebrands, J.‐L., Seelen, M. A. Critical role for complement receptor C5aR2 in the pathogenesis of renal ischemia‐reperfusion injury. FASEB J. 31, 3193–3204 (2017). www.fasebj.org
BACKGROUND.Systemic exposure to high-dose corticosteroids effectively combats acute rejection after kidney transplantation, but at the cost of substantial side effects. In this study, a murine acute ...renal allograft rejection model was used to investigate whether liposomal-encapsulated prednisolone (LP) facilitates local exposure to enhance its therapeutic effect.
METHODS.Male BalbC recipients received renal allografts from male C57BL/6J donors. Recipients were injected daily with 5 mg/kg cyclosporine A and received either 10 mg/kg prednisolone (P), or LP intravenously on day 0, 3, and 6, or no additional treatment. Functional magnetic resonance imaging (fMRI) was performed on day 6 to study allograft perfusion and organs were retrieved on day 7 for further analysis.
RESULTS.Staining of polyethylene-glycol-labeled liposomes and high performance liquid chromatography analysis revealed accumulation in the LP treated allograft. LP treatment induced the expression of glucocorticoid responsive gene Fkbp5 in the allograft. Flow-cytometry of allografts revealed liposome presence in CD45 cells, and reduced numbers of F4/80 macrophages, and CD3 T-lymphocytes upon LP treatment. Banff scoring showed reduced interstitial inflammation and tubulitis and fMRI analysis revealed improved allograft perfusion in LP versus NA mice.
CONCLUSIONS.Liposomal delivery of prednisolone improved renal bio-availability, increased perfusion and reduced cellular infiltrate in the allograft, when compared with conventional prednisolone. Clinical studies should reveal if treatment with LP results in improved efficacy and reduced side effects in patients with renal allograft rejection.
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