Spatial control of intracellular signaling relies on signaling proteins sensing their subcellular environment. In many cases, a large number of upstream signals are funneled to a master regulator of ...cellular behavior, but it remains unclear how individual proteins can rapidly integrate a complex array of signals within the appropriate spatial niche within the cell. As a model for how subcellular spatial information can control signaling activity, we have reconstituted the cell pole-specific control of the master regulator kinase/phosphatase CckA from the asymmetrically dividing bacterium Caulobacter crescentus. CckA is active as a kinase only when it accumulates within a microdomain at the new cell pole, where it colocalizes with the pseudokinase DivL. Both proteins contain multiple PAS domains, a multifunctional class of sensory domains present across the kingdoms of life. Here, we show that CckA uses its PAS domains to integrate information from DivL and its own oligomerization state to control the balance of its kinase and phosphatase activities. We reconstituted the DivL–CckA complex on liposomes in vitro and found that DivL directly controls the CckA kinase/phosphatase switch, and that stimulation of either CckA catalytic activity depends on the second of its two PAS domains. We further show that CckA oligomerizes through a multidomain interaction that is critical for stimulation of kinase activity by DivL, while DivL stimulation of CckA phosphatase activity is independent of CckA homooligomerization. Our results broadly demonstrate how signaling factors can leverage information from their subcellular niche to drive spatiotemporal control of cell signaling.
Little work has examined whether implicit evaluations can be effectively "undone" after learning new revelations. Across 7 experiments, participants fully reversed their implicit evaluation of a ...novel target person after reinterpreting earlier information. Revision occurred across multiple implicit evaluation measures (Experiments 1a and 1b), and only when the new information prompted a reinterpretation of prior learning versus did not (Experiment 2). The updating required active consideration of the information, as it emerged only with at least moderate cognitive resources (Experiment 3). Self-reported reinterpretation predicted (Experiment 4) and mediated (Experiment 5) revised implicit evaluations beyond the separate influence of how thoughtfully participants considered the new information in general. Finally, the revised evaluations were durable 3 days later (Experiment 6). We discuss how these results inform existing theoretical models, and consider implications for future research.
ABSTRACT
Experiential purchases (focused on doing rather than having) provide more satisfaction than material goods. Here, we examine a different downstream consequence of spending money on ...experiences: fostering social connection. Consumers reported feeling more kinship with someone who had made a similar experiential purchase than someone who had made a similar material purchase—a result tied to the greater centrality of experiences to one's identity. This greater sense of connection that experiences provide applied even when someone else had made a similar, but superior purchase. Participants also reported feeling more connected to others in general, not just those who have made the same purchase, when reflecting on experiential consumption—and these feelings of connection were expressed in a greater desire to engage in social activities when participants considered their experiential purchases than when they considered their material purchases. Together, these results demonstrate that experiential consumption enhances people's social connection quite broadly.
When and How Implicit First Impressions Can Be Updated Ferguson, Melissa J.; Mann, Thomas C.; Cone, Jeremy ...
Current directions in psychological science : a journal of the American Psychological Society,
08/2019, Letnik:
28, Številka:
4
Journal Article
Recenzirano
Human perceivers continually react to the social world implicitly—that is, spontaneously and rapidly. Earlier research suggested that implicit impressions of other people are slower to change than ...self-reported impressions in the face of contradictory evidence, often leaving them miscalibrated from what one learns to be true. Recent work, however, has identified conditions under which implicit impressions can be rapidly updated. Here, we review three lines of work showing that implicit impressions are responsive to information that is highly diagnostic, believable, or reframes earlier experience. These findings complement ongoing research on mechanisms of changing implicit impressions in a wider variety of groups, from real people to robots, and provide support for theoretical frameworks that embrace greater unity in the factors that can impact implicit and explicit social cognition.
Stereotypes are associations between social groups and semantic attributes that are widely shared within societies. The spoken and written language of a society affords a unique way to measure the ...magnitude and prevalence of these widely shared collective representations. Here, we used word embeddings to systematically quantify gender stereotypes in language corpora that are unprecedented in size (65+ million words) and scope (child and adult conversations, books, movies, TV). Across corpora, gender stereotypes emerged consistently and robustly for both theoretically selected stereotypes (e.g., work–home) and comprehensive lists of more than 600 personality traits and more than 300 occupations. Despite underlying differences across language corpora (e.g., time periods, formats, age groups), results revealed the pervasiveness of gender stereotypes in every corpus. Using gender stereotypes as the focal issue, we unite 19th-century theories of collective representations and 21st-century evidence on implicit social cognition to understand the subtle yet persistent presence of collective representations in language.
Highlights • An oscillating master genetic circuit temporally coordinates cell cycle modules. • A cellular module reflects a cascade of self-contained molecular events. • Cellular modules navigate ...shared spatial constraints via an intracellular compass. • Cytokinesis machinery reprograms cell polarity and cell type specific expression.
Recent work has shown that implicit first impressions of other people can be rapidly updated when new information about them is highly diagnostic or provides a reinterpretation of the basis of prior ...belief. The Affect Misattribution Procedure (AMP; Payne, Cheng, Govorun, & Stewart, 2005) is one prominent implicit measure that has been widely used in this and other work. However, the status of the AMP as a measure of unintentional responding has been a matter of debate, which necessarily also raises questions about the "implicitness" of the updated responses within recent person impression research. In re-analyses of published work, we identify multimodal distributions of AMP responses that raise concerns about potential intentional influences on this task. Drawing on 8 new studies, however, we find that such patterns are not likely attributable to intentional responding (Studies 1, 2A-2B), and that methodological modifications to the AMP procedure eliminate bimodality but do not eliminate effects of rapid revision (Studies 3A-6). Furthermore, these modifications provide evidence that the rapid-revision effects reported in earlier work can be produced under suboptimal conditions such as distraction and increased vigilance against prime influence. We advocate for the continued use of judgmental misattribution as a valuable tool in the arsenal of implicit social cognition researchers, but also encourage researchers to continue to examine the distributional patterns of measures like the AMP, and what those patterns might reflect.
Implicit evaluations (attitudes) are often described as resistant to change, especially when they were initially formed in a seemingly associative manner, such as via repeated evaluative pairings ...(REP), and new learning is created via propositional material, such as evaluative statements (ES). The present research (total N = 2,124) tested the responsiveness of implicit evaluations instantiated via REP to updating via different types of ES. In Experiment 1, initial learning was created via repeatedly pairing a novel target with strongly negative stimuli (screams) in an aversive REP (A-REP) task. Subsequent ES of opposing valence providing diagnostic information about the target's behavior substantially updated implicit (IAT) evaluations. In Experiment 2, behavioral ES resulted in successful updating after A-REP whether or not they provided an explanation for the initial A-REP learning. A previously unobtained result emerged in Experiment 3 showing that updating was durable even after 1 day. Finally, in Experiment 4, implicit evaluations were updated via diagnostic behavioral ES, but not via an ES instruction to suppose that different pairings had occurred during A-REP. Taken together, these experiments challenge associative theories of implicit evaluation by demonstrating that diagnostic behavioral statements can durably override the effects of initial learning on implicit evaluations, even if such initial learning is aversive and involves direct experience with stimulus pairings. Moreover, by showing that verbal manipulations based on diagnostic behavior but not a mere supposition instruction had impact, the present project advances theory by starting to identify the nature of learning that can adaptively update social impressions.
PDF
