Carbon monoxide dehydrogenase (CO-DH) in
Mycobacterium
sp. strain JC1 is a key enzyme for the carboxydotrophic growth, when carbon monoxide (CO) is supplied as a sole source of carbon and energy. ...This enzyme is also known to act as nitric oxide dehydrogenase (NO-DH) for the detoxification of NO. Several accessory genes such as
cutD, cutE, cutF, cutG, cutH
, and
cutI
, are clustered together with two copies of the CO-DH structural genes (
cutB1C1A1
and
cutB2C2A2
) in
Mycobacterium
sp. strain JC1 and are well conserved in carboxydotrophic mycobacteria. Transcription of the CO-DH structural and accessory genes was demonstrated to be increased significantly by acidified sodium nitrate as a source of NO. A
cutI
deletion (Δ
cutI
) mutant of
Mycobacterium
sp. strain JC1 was generated to identity the function of
CutI
. Lithoautotrophic growth of the Δ
cutI
mutant was severely affected in mineral medium supplemented with CO, while the mutant grew normally with glucose. Western blotting, CO-DH activity staining, and CO-DH-specific enzyme assay revealed a significant decrease in the cellular level of CO-DH in the Δ
cutI
mutant. Northern blot analysis and promoter assay showed that expression of the
cutB1
and
cutB2
genes was significantly reduced at the transcriptional level in the Δ
cutI
mutant, compared to that of the wildtype strain. The Δ
cutI
mutant was much more susceptible to NO than was the wild type.
La CUTI hizo otro planteo al Ministerio de Economia que consiste en que las inversiones que realizan las empresas de software en recursos humanos -es decir horas de trabajo de personal capacitado- ...sean elegibles para recibir los beneficios fiscales de la ley de Inversiones. "La ley de Inversiones no contempla la inversion en recursos humanos y en las empresas de tecnologia de la informacion el 90% de la inversion es en recursos humanos", senalo a El Pais el empresario del sector y vicepresidente de Nuevos Emprendimientos de la CUTI, Conrado Vina. Hasta ahora la CUTI no habia planteado que la industria del software pudiera ampararse en la ley de Inversiones ya que tenia una exoneracion propia. (c) 2011 NoticiasFinancieras - (c) 2011 GDA - El Pais - All rights reserved De la manera en que quedo armado el esquema tributario del sector y al no tener los compradores de software la posibilidad de recibir beneficios fiscales por esa inversion -como si lo tienen si compraran maquinaria u otro tipo de insumos-, hace que a las empresas que producen software les sirva mas "exportar que vender aca", dijo a El Pais el empresario del sector y vicepresidente de Nuevos Emprendimientos de la Camara Uruguaya de Tecnologias de la Informacion (CUTI), Conrado Vina. Con el actual esquema "no se le esta facilitando" a las empresas uruguayas adquirir tecnologias de la informacion, senalo. Ademas a quienes producen software "les conviene mas venderle" a empresas del exterior que son "competidores" de las uruguayas, lo que "indirectamente las perjudica", apunto Vina.
Objective: The aim of this study was to determine the clinical outcome, microbiological outcome and safety profile of CSE-1034, a novel combination of Ceftriaxone, Sulbactam and EDTA in patients with ...complicated urinary tract infections (cUTI). Materials and Methods: This was a randomized, controlled, open-labeled Phase-3 trial with the primary objective of assessing the efficacy and safety of CSE-1034 versus Ceftriaxone for the empirical treatment of cUTI. Adult cUTI patients were randomized to receive either intravenous dose of CSE-1034 or Ceftriaxone. The primary end point was composite cure rate (clinical response and bacterial eradication) in mMITT population at test of cure (TOC) visit. Secondary measures included verification of primary endpoint across other visits in different population sets, safety of patients and treatment duration. Results: Overall, 204 patients were enrolled in the study and received one of the two treatments. At primary endpoint (TOC visit), the composite cure rate was much higher in CSE-1034 treatment arm compared to Ceftriaxone arm i.e. 97% (68/70) vs 83% (58/71) (treatment difference 12.6%; 95% CI: 5.9% to 26.4%). The adverse events (AEs) rates reported in two treatment arms were 21% in CSE-1034 and 36% in Ceftriaxone groups. Additionally, the treatment duration in CSE-1034 arm was significantly less (P < 0.05). Conclusions: CSE-1034 3 g every 24 h showed a high favorable clinical and bacteriological response, and 95% CI around the treatment difference prove the superiority of CSE-1034 vs. Ceftriaxone for the treatment of cUTI. Therefore, CSE-1034 provides an effective alternative in the treatment of patients with cUTI.
Fluoroquinolones are a guideline-recommended therapy for complicated urinary tract infections, including pyelonephritis. Elevated drug concentrations of fluoroquinolones in the urine and therapy with ...high-dose levofloxacin are believed to overcome resistance and effectively treat infections caused by resistant bacteria. The ASPECT-cUTI phase 3 clinical trial (ClinicalTrials.gov, NCT01345929 and NCT01345955 , both registered April 28, 2011) provided an opportunity to test this hypothesis by examining the clinical and microbiological outcomes of high-dose levofloxacin treatment by levofloxacin minimum inhibitory concentration.
Patients were randomly assigned 1:1 to ceftolozane/tazobactam (1.5 g intravenous every 8 h) or levofloxacin (750 mg intravenous once daily) for 7 days of therapy. The ASPECT-cUTI study provided data on 370 patients with at least one isolate of Enterobacteriaceae at baseline who were treated with levofloxacin. Outcomes were assessed at the test-of-cure (5-9 days after treatment) and late follow-up (21-42 days after treatment) visits in the microbiologically evaluable population (N = 327).
Test-of-cure clinical cure rates above 90% were observed at minimum inhibitory concentrations ≤4 μg/mL. Microbiological eradication rates were consistently >90% at levofloxacin minimum inhibitory concentrations ≤0.06 μg/mL. Lack of eradication of causative pathogens at the test-of-cure visit increased the likelihood of relapse by the late follow-up visit.
Results from this study do not support levofloxacin therapy for complicated urinary tract infections caused by organisms with levofloxacin minimum inhibitory concentrations ≥4 μg/mL.
ClinicalTrials.gov, NCT01345929 and NCT01345955.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, SIK, UILJ, UKNU, UL, UM, UPUK
Pasal 70 ayat (3) UU Nomor 10/2016 tentang Pilkada mewajibkan kepala daerah incumbent untuk cuti jika yang bersangkutan akan mencalonkan kembali pada daerah yang sama. Terhadap aturan tersebut ...kemudian menimbulkan pro dan kontra, apakah aturan cuti kampanye bagi kepala daerah incumbent layak dipertahankan atau tidak. Setiap peraturan pasti memiliki kelebihan dan kelemahan termasuk peraturan tentang cuti ini terutama jika dikaitkan dengan tujuan dari pilkada itu sendiri. Rumusan masalahnya:, Pertama, Bagaimana pengaturan persyaratan cuti kampanye bagi kepala daerah incumbent dalam UU Nomor 10/2016 ? Kedua, Apa kelebihan dan kelemahan adanya pengaturan persyaratan cuti kampanye bagi kepala daerah incumbent dalam pilkada? Penelitian ini adalah penelitian yuridis-normatif dengan menggunakan dua pendekatan yaitu pendekatan kasus dan pendekatan perundang-undangan. Penelitian ini menyimpulkan, bahwa pertama pasal 70 ayat (3) UU 10/2016 telah menimbulkan problematika hukum. Kedua, terdapat kelebihan dan kelemahan terhadap aturan cuti kampanye. Salah satu kelebihannya ialah untuk membatasi kekuasaan kepala daerah agar tidak terjadi penyalahgunaan kekuasaan, sedangkan salah satu kelemahannya bahwa kewajiban untuk cuti dapat merugikan hak kepala daerah incumbent untuk bekerja menuntaskan amanah rakyat hasil pemilihan langsung serta merugikan rakyat pemilih.
The chemical bonding in the intermetallic compounds ZnPd and ZnPt with the tetragonal CuTi type of structure, and in the chemically related intermetallic compounds ZnNi, ZnCu, ZnAg, and ZnAu with the ...cubic CsCl type of structure, is examined applying the electron localizability approach. The analysis of the chemical bonding reveals charge transfer from zinc to the transition metal (TM), in line with the respective electronegativities. While in ZnNi, ZnCu, and ZnAg as well as ZnPd and ZnPt the Zn–TM interactions are described by multi‐center bonding and ionic interactions, mainly ionic interactions are found in the intermetallic compound ZnAu. Going from ZnNi over ZnPd to ZnPt, a gradual change from isotropic to more and more anisotropic distribution of the atomic interactions is detected, resulting in a more pronounced tetragonal distortion.
Objectives:
A large retrospective database study was conducted to assess the incidence rate of treatment-emergent renal impairment/failure, seizure, and hemolytic anemia in doripenem and imipenem ...intravenous (IV)-exposed patients treated for complicated urinary tract infection (cUTI) and complicated intra-abdominal infection (cIAI) in US hospitals.
Methods:
Using the Premier Perspective™ Database (PPD), which maintains hospital discharge records for over 309 million patients, the incidence rate of treatment-emergent renal impairment/failure, seizure, and hemolytic anemia in the doripenem-treated compared with imipenem IV-treated population was examined.
Results:
The unadjusted doripenem rate ratio (RR) for renal impairment/failure relative to imipenem IV was 1.13 95% confidence interval (CI) 1.07–1.21; p < 0.0001. The unadjusted doripenem rate ratio for seizure relative to imipenem IV was 0.74 (95% CI 0.52–1.05; p = 0.07). In the comparative safety analysis, adjusted incidence rates of renal impairment/failure between doripenem-exposed patients and a propensity score-matched comparator cohort of imipenem IV-exposed patients showed no statistically significant difference in cUTI RR = 1.02; 95% CI 0.93–1.12; p = 0.71 or cIAI (RR = 1.17; 95% CI 1.00–1.36; p = 0.05). Likewise, there was no statistically significant difference in adjusted incidence rates for seizures in doripenem-treated versus matched imipenem-treated patients for cUTI (RR = 0.69; 95% CI 0.41–1.14; p = 0.15) or cIAI (RR = 0.45; 95% CI 0.15–1.41; p = 0.17). No hemolytic anemia events were observed in this study.
Conclusions:
In this large retrospective cohort study of US hospitalized patients, no statistically significant differences in the adjusted relative rates of renal impairment/failure and seizure were observed between doripenem and a propensity score-matched comparator cohort of imipenem IV patients in the treatment of cUTI and cIAI.
Recently, new compounds Mn2RuSn and Mn2RuSi were synthesized and their crystal structures were studied by X-ray diffraction measurements. The measurements showed that they have a Heusler-like cubic ...structure, but the details have been unclear so far. Therefore, to clarify their atomic and magnetic order, we have carried out first-principles total-energy calculations for several different atomic arrangements, together with ferrimagnetic and ferromagnetic orders. The comparison among total energies shows that the most stable structure is ferrimagnetic Hg2CuTi one in both Mn2RuSn and Mn2RuSi. It is also found that the compound Mn2RuSi with a Hg2CuTi structure could be a half-metallic ferrimagnet.
He-GTA welding of 10mm thick copper plates by using independent developed welding material - CuTi alloys was investigated. The crystal crack induced by eutectic of Cu and Cu2O is disappeared ...completely as the addition of deoxidant element Ti. It can be concluded that the Ti inhibits the formation of Cu2O and combines with O to generate TiO2 through the thermal dynamical calculation. As the TiO2 has the characteristic of high molt point and low density, TiO2 flows upward to surface of molten pool during welding and forms as weld slag at the weld surface after cooling, then the uniform microstructure without crystal crack is obtained finally. In all GTA welding specimens tested, the failure is located in the heat affected zone and the tensile strength is similar to the base metal.
A New Ternary Titanium-based Heusler Alloy Wang, Fei; Feng, Lei; Zhang, Dongmei ...
Journal of superconductivity and novel magnetism,
03/2014, Letnik:
27, Številka:
3
Journal Article
Recenzirano
A new
Heusler
alloy V
2
NiSb with Hg
2
CuTi-
type
structure has been obtained by means of first-principles calculations. The electric structure and magnetic properties of the alloy have been also ...investigated. The results show that the alloy has a total magnetic moment of 0.82
μ
B
per unit cell on first-principles calculations. The magnetic moment of atom Ni is 0.13
μ
B
; the magnetic moments of
V
(1) atom and
V
(2) atom are −0.48
μ
B
and 0.76
μ
B
, respectively, indicating that the alloy is a antiferrimagnet.
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