Vanden Brugge (Jan Isaac), says Pontanus. Album amicorum (1591-1627)
Scanning from an original document.
Scanning from a substitute document: R 59779.
Initials: G. M. = Giampietro Mesmes, i.e. ...Jean-Pierre de Mesmes (1559).
Vanden Brugge (Jan Isaac), says Pontanus. Album amicorum (1591-1627)
Vanden Brugge (Jan Isaac), dit Pontanus. Album amicorum (1591-1627)
Numérisation effectuée à partir d'un document original.
Numérisation effectuée à partir d'un document de substitution : R 59779.
Jan Isaac Vanden Brugge, né à Elseneur le 21 janvier 1571, mort à Harderwyck le 6 octobre 1639, s'est fait connaître à la fois comme historien et comme médecin. On trouvera dans la Nouv. Biographie générale (t. XL, col. 770) une liste sommaire de ses ouvrages. Son album se compose de 13 f. entourés d'un encadrement gravé. J. van Dam, qui l'avait acquis en 1826, l'a interfolié pour y ajouter des notices sur les amis de Pontanus, et il a numéroté les feuillets de 1 à 28.Les inscriptions sont au nombre de 26 ; en voici le détail :Bèze (Théodore de), « Genevae, annum agens hujus vitae 83 », 9 oct. 1601, fol. 15.Brinck (Ernest), « in Angliam concessurus », Harderwyck, 20 juill. 1607, fol. 27 v°.Cambden (William), « Anglus », 8 sept. 1596, fol. 5 v°.Canter (Jan), fils de Théodore, Rome, 29 janv. 1593, fol. 27.Canter (Lambert), fils de Théodore, Rome, 1593, fol. 3.Cherler (Johann Heinrich), de Bâle, philosophe et médecin, Montbéliard, 15 mai 1601, fol. 13 v°.Dhona (Achatius Burggraff zu) der Elter, 3/15 mars 1625, fol. 15.Dousa. Voy. Vander Doese.Fischer (Johann), dit Piscator, Herborn, 7 sept. 1600, fol. 23 v°.Gruter (Jan), ou Gruytere, dit Gruterus, Heidelberg, 1er oct. 1600, fol. 3 v°.Heile (Peeter van), « Belga », Draxholm en Danemark, 8 juill. 1599, fol. 9 v°.Lemann (Burckhard), de Zürich, ministre, aetatis 71, 1601, fol. 15 v°.Lipse (Juste), 13 mars 1596, fol. 17.Piscator (Joh.). Voy. Fischer.Pynacker (Corneille), de Delft, Leide, 1595, fol. 7.Raphelenghien (François van), 26 nov. 1600 (pridie ante inexpectatum moestissimumque suum a Basilea discessum), fol. 11.Sattler (Wolfgang), étudiant ès arts, en médecine et en droit, Bâle, 1600, fol. 19 v°.Scaliger (Joseph), fils de Jules-César, Leide, 7 sept. 1595, fol. 13.Schougart (Georg), Bâle, 28 mars 1601, fol. 12 v°.Soping (Geoffroi), Franeker, 19 mars 1592, fol. 25.Stock (Richard), « Stockaeus », Amsterdam, 15 mai 1592, fol. 5.Stück (Johann Wilhelm), « Stuckius », de Zürich, 9 août 1601, fol. 7 v°.Thomson (Richard), Cambridge, 20 mars 1596, fol. 19.Tiling (Johann), Rostock, vers 1595 (?), fol. 25.Vander Doese (Frans), dit Dousa, Leide, 23 oct. 1597, fol. 21 v°.Vander Doese (Jan), dit Dousa, « Nordovix », 2 août 1595, fol. 21.Vander Doese (Jan), dit Dousa, fils, « Nordovix », 6 mai 1592. fol. 17 v°.Witfeld (Arnold), Copenhague, 1595, fol. 9.Au XVIIIe siècle cet album appartint au physicien P. VAN MUSSCHENBROEK, mort en 1761 et dont la bibliothèque fut vendue à Leide en 1826. Il fut alors acquis par I. VAN DAM. Il a figuré en dernier lieu à la vente d'ERNEST STROEHLIN (Cat., 1912, n° 994).
Initiales : G. M. = Giampietro Mesmes, c'est-à-dire Jean-Pierre de Mesmes (1559).
Vanden Brugge (Jan Isaac), dit Pontanus. Album amicorum (1591-1627)
Vanden Brugge (Jan Isaac), dit Pontanus. Album amicorum (1591-1627)
Numérisation effectuée à partir d'un document original.
Numérisation effectuée à partir d'un document de substitution : R 59779.
Jan Isaac Vanden Brugge, né à Elseneur le 21 janvier 1571, mort à Harderwyck le 6 octobre 1639, s'est fait connaître à la fois comme historien et comme médecin. On trouvera dans la Nouv. Biographie générale (t. XL, col. 770) une liste sommaire de ses ouvrages. Son album se compose de 13 f. entourés d'un encadrement gravé. J. van Dam, qui l'avait acquis en 1826, l'a interfolié pour y ajouter des notices sur les amis de Pontanus, et il a numéroté les feuillets de 1 à 28.Les inscriptions sont au nombre de 26 ; en voici le détail :Bèze (Théodore de), « Genevae, annum agens hujus vitae 83 », 9 oct. 1601, fol. 15.Brinck (Ernest), « in Angliam concessurus », Harderwyck, 20 juill. 1607, fol. 27 v°.Cambden (William), « Anglus », 8 sept. 1596, fol. 5 v°.Canter (Jan), fils de Théodore, Rome, 29 janv. 1593, fol. 27.Canter (Lambert), fils de Théodore, Rome, 1593, fol. 3.Cherler (Johann Heinrich), de Bâle, philosophe et médecin, Montbéliard, 15 mai 1601, fol. 13 v°.Dhona (Achatius Burggraff zu) der Elter, 3/15 mars 1625, fol. 15.Dousa. Voy. Vander Doese.Fischer (Johann), dit Piscator, Herborn, 7 sept. 1600, fol. 23 v°.Gruter (Jan), ou Gruytere, dit Gruterus, Heidelberg, 1er oct. 1600, fol. 3 v°.Heile (Peeter van), « Belga », Draxholm en Danemark, 8 juill. 1599, fol. 9 v°.Lemann (Burckhard), de Zürich, ministre, aetatis 71, 1601, fol. 15 v°.Lipse (Juste), 13 mars 1596, fol. 17.Piscator (Joh.). Voy. Fischer.Pynacker (Corneille), de Delft, Leide, 1595, fol. 7.Raphelenghien (François van), 26 nov. 1600 (pridie ante inexpectatum moestissimumque suum a Basilea discessum), fol. 11.Sattler (Wolfgang), étudiant ès arts, en médecine et en droit, Bâle, 1600, fol. 19 v°.Scaliger (Joseph), fils de Jules-César, Leide, 7 sept. 1595, fol. 13.Schougart (Georg), Bâle, 28 mars 1601, fol. 12 v°.Soping (Geoffroi), Franeker, 19 mars 1592, fol. 25.Stock (Richard), « Stockaeus », Amsterdam, 15 mai 1592, fol. 5.Stück (Johann Wilhelm), « Stuckius », de Zürich, 9 août 1601, fol. 7 v°.Thomson (Richard), Cambridge, 20 mars 1596, fol. 19.Tiling (Johann), Rostock, vers 1595 (?), fol. 25.Vander Doese (Frans), dit Dousa, Leide, 23 oct. 1597, fol. 21 v°.Vander Doese (Jan), dit Dousa, « Nordovix », 2 août 1595, fol. 21.Vander Doese (Jan), dit Dousa, fils, « Nordovix », 6 mai 1592. fol. 17 v°.Witfeld (Arnold), Copenhague, 1595, fol. 9.Au XVIIIe siècle cet album appartint au physicien P. VAN MUSSCHENBROEK, mort en 1761 et dont la bibliothèque fut vendue à Leide en 1826. Il fut alors acquis par I. VAN DAM. Il a figuré en dernier lieu à la vente d'ERNEST STROEHLIN (Cat., 1912, n° 994).
Initiales : G. M. = Giampietro Mesmes, c'est-à-dire Jean-Pierre de Mesmes (1559).
Manuscrits de la collection James de Rothschild.
Vanden Brugge (Jan Isaac), dit Pontanus. Album amicorum (1591-1627)
Kalthüber (Bartholomaeus). Album amicorum, ayant servi aussi à Johann Rüdiger (1566-1567)
Rüdiger (Johann). Album amicorum ayant servi aussi à Barth. Kalthüber (1566-1567)
Numérisation effectuée à ...partir d'un document original.
Ce petit volume a été fait pour deux amis qui ont recueilli des inscriptions dédiées, tantôt à l'un, tantôt à l'autre, quelquefois à tous deux. Plus tard, il appartint à Andreas Poget (fol. 20 v°), à Paul Puschnik, « ludi moderator monasterii Victorii », 1573 (fol. 20 v°, 43), à Georg Bihler (fol. 44 v°), à Johann Friedrich, comte de Lemberg (fol. 53 v°), à Mathias von Münding, d'abord candidat au doctorat en droit (fol. 53 v°), puis docteur ès droits et gouverneur des pages du roi de Hongrie et de Bohème (fol. 48, 51), et secrétaire du roi (fol. 54).Voici une table alphabétique des personnages qui se sont inscrits dans l'album :Ackermann (Johann), dit Agricola, d'Amberg, Rastadt, 4 juin 1567, fol. 33 V°.Brassicanus. Voy. Kuhlburger.Caloburraeus. Voy. Schönpichler.Förter (Sebastian), pour Joh. Rüdiger, 1567, fol. 14 v°.Firinus (Eucharius), de Homberg, pour B. Kalthüber, fol. 21.Harzer (Wolfgang), de Graz en Styrie, pour Kalthüber et pour Rudiger, 17 nov. 1567, fol. 38 v°.Herndt (Georg), pour les mêmes, 1567, fol. 7.Initiales :B. H., pour J. Rüdiger, 1561, fol. 29 v°.Kalthüber (Bartholomaeus), de Wotzperg, pour J. Rüdiger, fol. 29.Kohlburger (Heinrich), dit Brassicanus, de Schmalkalde, pour J. Rüdiger, Strasbourg, 18 déc. 1566, fol. 26 v°.Lay (Konrad), de Crailsheim, pour J. Rüdiger, Strasbourg, déc. 1566, fol. 40 v°.Laun (Hieronymus), de Salzbourg, pour Kalthüber et Rüdiger, 15 mars 1568, fol. 15.Michel (Kaspar), « Cealophanus », Strasbourg, déc. 1566, fol. 30.Neuschwert (Mathias), Autrichien, pour Kalthüber et Rüdiger, 2 déc. 1567, fol. 24.Petrowsky (Stanislaw), pour Georg Bihler, fol. 44 v°.Pfeiffer (Baumhardt). « Pheiffer », 26 oct. 1578, fol. 17.Philipp (Frère), « dominicanus conventualis in monasterio Victoriensi », fol. 25 v°.Reichlin (Anton), « Dunzenheimensis pastor », fol. 19 v°.Renner (Nicodemus), pour Andreas Poget et Paul Puschnik, nov. 1573, fol. 20 v°.Roller (Georg), pour Kalthüber et pour Rüdiger, fol. 40.Rot (Michel), pour J. Rüdiger, fol. 9.Rüdiger (Johann), « Rüdinger, Schofflenzianus, olim pastor in Schyersheim », 1566 (?), pour B. Kalthüber, fol. 4.Schmaltz (Martin), de Nuremberg, pour Rüdiger, 1567, fol. 41 v°.Schönpichler (Karl), dit Caloburraeus, de Vienne, pour Paul Puschnik, fol. 43.Stock (Friedrich), « Neuenmusensis », pour J. Rüdiger, fol. 35.Wannesiegler (Philipp), pour B. Kalthüber et J. Rüdiger, fol. 36 v°.Winckh (Ferdinand von), « Carinthius », pour J. Rüdiger, Strasbourg, déc. 1566, fol. 12.Mathias von Münding, qui posséda le petit volume au XVIIe siècle, y a inséré une curieuse liste dus pages formée par lui. Nous la transcrivons ici :Fol. 49. Nomina meorum epheborum.Carolus Eugenius, marchio Badensis et S. R. I. princeps.Franciscus Christophorus Kevenhiller, comes a Franckhenburg.Ferdinandus Emericus, comes a Kollonitz.Leopoldus Wilmhelmus, comes a Kollonitz.Lobgott, comes a Kueffstein.Ehrgott, comes a Kueffstein.Leopoldus Rodericus comes de Collowrat.v° Joannes Borbianus Belgioioso, comes Mediolanensis.Michael Robatta, comes Forum sic Juliensis.Ludovicus Franciscus liber baro a Zyvotin, Moravus.Henricus Fridericus, comes a Guttenstern, Bohemus.Joannes Georgius Strein, liber baro Bohemus.Joannes Maximilianus Stribech, liber baro Slivy.Anno 1654 fuerunt ephebi Ferdinandi IV, Romanorum regis defuncti.Fol. 51. Serenissimi Leopoldi, archiducis, regis Hungariae et Bohemiae, postea Romanorum imperatoris, anno 1657 :Ferdinandus Christophorus Teuffel, liber baro a Günterstorff, Austriacus.Franciscus Augustinus comes a Thun, Tyrolensis.Claudius Josephus Leopoldus, liber baro de Montroschier, Burgundus.Carolus Julius Pozzecky, liber baro Moravus.v° Christophorus Hortmannus, liber baro a Schallenberg, Austriacus.Joannes Georgius Strein, liber baro a Schwartzenau, Bohemus.Joannes Maximilianus, liber baro ab Althan, Austriacus.Romboldus Philippus Antonius Conod a Porzia, Forum sic Juliensis.Maximilianus a Schleinitz, liber baro Bohemus.Fol. 52. Carolus Conov, eques Hibernus.Franciscus Carolus Wisching, liber baro Austriacus.Joannes Andreas ab Himmelberg, eques Charyntius.Franciscus Tauffe, eques Hibernus.Josephus Maria Vice Comes, Mediolanensis.MATTHIAS DE MÜNDING, eorum praefectus.Un des derniers propriétaires de l'album, le peintre ACHILLE DEVERIA, mort en 1857, a exécuté sur chacun des plats extérieurs un dessin à la plume. Le volume a fait partie, en dernier lieu, des bibliothèques de HENRI BORDIER et de son gendre E. STROEHLIN (Cat., 1912, n°).
Initiales : B. H. : (étudiant allemand) inscription dans un album (1561).
Kalthüber (Bartholomaeus). Album amicorum, ayant servi aussi à Johann Rüdiger (1566-1567)
Manuscrits de la collection James de Rothschild.
Rüdiger (Johann). Album amicorum ayant servi aussi à Barth. Kalthüber (1566-1567)
Kalthüber (Bartholomaeus). Album amicorum, having also served Johann Rüdiger (1566-1567)
Rüdiger (Johann). Album amicorum also used in Barth. Kalthüber (1566-1567)
Scanning from an original document.
This small volume was made for two friends who gathered dedicated inscriptions, sometimes at one, sometimes at the other, sometimes at both. Later, it appeared to Andreas Poget (fol. 20 v °), Paul Puschnik, “ludi moderator monasterii Victorii,” 1573 (fol. 20 v °, 43), Georg Bihler (fol. 44 v °), Johann Friedrich, Count of Lemberg (fol. 53 v °), Mathias von Münding, first candidate for the doctorate in law (fol. 53 v °), then Doctor of Rights and Governor of the pages of the king of Hungary and Bohemia (fol. 48, 51), and secretary.Below is an alphabetical table of the figures who have inscribed themselves in the album: Ackermann (Johann), known as Agricola, dAmberg, Rastadt, 4 June 1567, fol. 33 V °.Brassicanus. Voy. Kuhlburger.Caloburraeus. Voy. Schönpichler.Förter (Sebastian), for Joh. Rüdiger, 1567, fol. 14 v °.Firinus (Eucharius), of Homberg, for B. Kalthüber, fol. 21.Harzer (Wolfgang), from Graz to Styria, for Kalthüber and for Rudiger, 17 nov. 1567, fol. 38 v °.Herndt (Georg), for the same, 1567, fol. 7.Initials: B. H., for J. Rüdiger, 1561, fol. 29 v °.Kalthüber (Bartholomaeus), from Wotzperg, for J. Rüdiger, fol. 29.Kohlburger (Heinrich), says Brassicanus, of Schmalkalde, for J. Rüdiger, Strasbourg, 18 dec. 1566, fol. 26 v °.Lay (Konrad), by Crailsheim, for J. Rüdiger, Strasbourg, dec. 1566, fol. 40 v °.Laun (Hieronymus), from Salzburg, for Kalthüber and Rüdiger, 15 March 1568, fol. 15.Michel (Kaspar), “Cealophanus,” Strasbourg, dec. 1566, fol. 30.Neuschwert (Mathias), Austrian, for Kalthüber and Rüdiger, 2 dec. 1567, fol. 24.Petrowsky (Stanislaw), for Georg Bihler, fol. 44 v °.Pfeiffer (Baumhardt). “Pheiffer,” Oct 26. 1578, fol. 17.Philipp (Brother), “dominicanus conventualis in monasterio Victoriensi,” fol. 25 v °.Reichlin (Anton), “Dunzenheimensis pastor,” fol. 19 v °.Renner (Nicodemus), for Andreas Poget and Paul Puschnik, nov. 1573, fol. 20 v °.Roller (Georg), for Kalthüber and for Rüdiger, fol. 40.Rot (Michel), for J. Rüdiger, fol. 9.Rüdiger (Johann), “Rüdinger, Schofflenzianus, olim pastor in Schyersheim,” 1566 (?), for B. Kalthüber, fol. 4.Schmaltz (Martin), from Nuremberg, for Rüdiger, 1567, fol. 41 v °.Schönpichler (Karl), known as Caloburraeus, from Vienna, for Paul Puschnik, fol. 43.Stock (Friedrich), “Neuenmusensis,” for J. Rüdiger, fol. 35.Wannesiegler (Philipp), for B. Kalthüber and J. Rüdiger, fol. 36 v °.Winckh (Ferdinand von), “Carinthius,” for J. Rüdiger, Strasbourg, dec. 1566, fol. 12.Mathias von Münding, who owned the small volume in the seventeenth century, inserted a curious list of pages formed by him. We transcribe it here: Fol. 49. Nomina meorum epheborum.Carolus Eugenius, marchio Badensis and S. R. I. princeps.Franciscus Christophorus Kevenhiller, comes a Franckhenburg.Ferdinandus Emericus, comes a Kollonitz.Leopoldus Wilmhelmus, comes a Kollonitz.Lobgott, comes a Kueffstein.Ehrgott, comes a Kueffstein.Leopoldus Rodericus comes from Collowrat.v ° Joannes Borbianus Belgioioso, comes Mediolanensis.Michael R 51. Serenissimi Leopoldi, archiducis, regis Hungariae and Bohemiae, postea Romanorum imperatoris, anno 1657: Ferdinandus Christophorus Teuffel, liber baro a Günterstorff, Austriacus.Franciscus Augustinus comes a Thun, Tyrolensis.Claudius Josephus Leopoldus, liber baro de Montroschier, Burgundus.Carolus Julius Pozzeck 52. Carolus Conov, eques Hibernus.Franciscus Carolus Wisching, liber baro Austriacus.Joannes Andreas ab Himmelberg, eques Charyntius.Franciscus Tauffe, eques Hibernus.Josephus Maria Vice Comes, Mediolanensis.MATTHIAS DE MÜNDING, eorum praefectus.One of the last owners of the album, the painter ACHILLE DEVERIA, who died in 1857, a The volume was part of the libraries of HENRI BORDIER and his son-in-law E. STROEHLIN (Cat., 1912, n °).
Initials: B. H. (German student) inscription in an album (1561).
Kalthüber (Bartholomaeus). Album amicorum, having also served Johann Rüdiger (1566-1567)
Kalthüber (Bartholomaeus). Album amicorum, ayant servi aussi à Johann Rüdiger (1566-1567)
Rüdiger (Johann). Album amicorum ayant servi aussi à Barth. Kalthüber (1566-1567)
Numérisation effectuée à partir d'un document original.
Ce petit volume a été fait pour deux amis qui ont recueilli des inscriptions dédiées, tantôt à l'un, tantôt à l'autre, quelquefois à tous deux. Plus tard, il appartint à Andreas Poget (fol. 20 v°), à Paul Puschnik, « ludi moderator monasterii Victorii », 1573 (fol. 20 v°, 43), à Georg Bihler (fol. 44 v°), à Johann Friedrich, comte de Lemberg (fol. 53 v°), à Mathias von Münding, d'abord candidat au doctorat en droit (fol. 53 v°), puis docteur ès droits et gouverneur des pages du roi de Hongrie et de Bohème (fol. 48, 51), et secrétaire du roi (fol. 54).Voici une table alphabétique des personnages qui se sont inscrits dans l'album :Ackermann (Johann), dit Agricola, d'Amberg, Ra
Display omitted
•A new nanosensor based on BSA capped-CuNCs for MGF detection was developed.•The proposed nanosensor showed excellent performance in sensing of MGF.•The mechanism of the selective ...recognition of MGF by the CuNCs@BSA sensor was proposed.•The nanosensor was applied in real samples with good recoveries.
In this paper we report for the first time a fluorescence nanosensor for the detection of trace amount of mangiferin (MGF) by using bovine serum albumin (BSA)-protected copper nanoclusters (CuNCs@BSA). The CuNCs@BSA have an average size of 1.4±0.2nm and show a blue emission at 640nm. Fluorescence emission of CuNCs@BSA was quenched in the presence of MGF providing a fluorescence responsive probe in a linear range of 3–180μM with a detection limit of 210nM calculated at a signal-to-noise ratio of 3. The CuNCs@BSA show high photostability, since after 50min of irradiation, only a fluorescence decrease of 15% is appreciated. Interference and competition studies indicate that the nanoprobe presents good selectivity over other relevant molecules including flavonoids and metal ions. These results confirmed that the nanosensor has high selectivity towards MGF in the presence of other substances. In addition the mechanism of sensitive fluorescence quenching response of CuNCs@BSA to MGF has been discussed. The sensor was then applied to the analysis of MGF in African mango extract and satisfactory recoveries were obtained indicating a good accuracy and reproducibility of the fluorescence nanosensor for detection of MGF. The proposed sensor is simple, rapid, and cost-effective demonstrating great potential for the determination of MGF in real samples.
Bovine serum albumin (BSA) was firstly implemented as an effective sensitivity enhancer for a peptide-based amperometric biosensor for the ultrasensitive detection of prostate specific antigen (PSA). ...A porous and conductive substrate of chitosan-lead ferrocyanide-(poly(diallyldimethylammonium chloride)-graphene oxide) was in-situ generated on a glassy carbon electrode (GCE), in which Pb2Fe(CN)6 served as a novel redox species with strong current signal at −0.46 V (vs. Ag/AgCl). Poly(diallyldimethylammonium chloride)-graphene oxide was applied to improve conductivity of the substrate. After adsorbing Pb2+ for signal amplification, chitosan provided active sites to simultaneously immobilize peptides and 1-aminopropyl-3-methylimidazolium chloride by glutaraldehyde. To enhance the sensitivity, BSA was chemically linked to the immobilized peptide, behaving as a serious decrease of current signal for BSA hindering the electron transfer. The dramatic increase of current signal of the biosensor was obtained by PSA cleaving the immobilized BSA-peptide. The proposed biosensor exhibited a detection limit of 1fgmL−1 for PSA and its sensitivity was seven-fold higher than previous works.
•BSA was firstly implemented as an effective sensitivity enhancer for the peptide-based amperometric biosensor.•Multiple amplification strategies were developed for the amperometric biosensor for PSA.•Chitosan-Pb2Fe(CN)6-PDDA-GO as a new redox species was used as substrate.
Abstract Human serum albumin (HSA) is a biological nanocarrier that forms non-covalent complexes with a number of synthetic and biomolecules. Previously we demonstrated radiolabeled HSA-based ...nanoparticles can form non-covalent complexes with fluorescent cyanine dyes yielding imaging agents for surgical guidance towards tumor draining lymph nodes. Here the self-assembly approach enabled rapid clinical translation. Based on this experience we reasoned it would be interesting to expand this non-covalent technology to a targeted approach. Therefore, the ability of HSA to form non-covalent self-assembled complexes with peptides via near-infrared (NIR) cyanine dyes was explored. Föster resonance energy transfer (FRET) quenching interactions between HSA-Cy5 and the non-covalently bound fluorescent molecules indocyanine green (ICG), IR783–CO2 H and three IR783-labeled targeting peptides were used to monitor complex assembly and disassembly. The host-guest interactions between HSA and IR783-labeled peptides enabled the formation of (bio)nanoparticles that are coated with peptides, which may target αv β3 -integrins, the chemokine receptor 4 (CXCR4), or somatostatin receptors. The potential of CXCR4-targeted (bio)nanoparticles in sentinel lymph node procedures is demonstrated in vivo. By non-covalently binding NIR-dye labeled peptides to an already clinically approved HSA-scaffold, we have readily formed targeted bionanoparticles.
The effective removal of heavy metals and soluble microbial products from wastewater is crucial for ensuring a safe environment and good quality human health. The present work investigated the ...potential of eggshell (ES) waste as an adsorbent for removing heavy metals and soluble microbial products. ES was firstly used to capture heavy metal ions, and the eggshell–metal (ES-M) complex was then applied to remove soluble microbial products (e.g., proteins) from aqueous solution. In this study, bovine serum albumin (BSA) was selected as a model protein-based contaminant. The equilibrium and kinetic characteristics of soluble protein removed by ES were evaluated in batch mode involving parameters such as metal ions (Cu
2+
, Zn
2+
, Ni
2+
, Co
2+
), operating temperatures (277–323 K), and particle size of ES (100–700 µm). The isotherm curves were well-fitted by Langmuir–Freundlich model. As the temperature increased from 277 to 323 K, the maximum binding capacity for BSA increased from 25.22 to 34.28 (mg BSA/g ES-Zn). The negative values of Δ
G
° indicated the spontaneous nature of the protein adsorption, while the kinetic of protein adsorption followed the pseudo-second-order model. ES functionalized with heavy metal ions acted as an effective pseudo-chelating adsorbent for the removal of soluble protein from wastewater. Chelates of Zn–BSA found on the ES complexes were found to be highly stable, indicating a minimal possibility of secondary pollution caused by these Zn- and BSA-containing ES complexes. The ES-Zn complex can be potentially used as an adsorbent for removing soluble microbial products in wastewater prior to the membrane filtration.
Graphic abstract
Purpose
This paper aims to investigate the immunoinhibitory properties of a lymph nodes-targeting suppressive oligonucleotide (ODN) for the potential treatment of autoimmune diseases or chronic ...inflammation.
Methods
Synthetic suppressive ODN engineered with an albumin-binding diacyl lipid at the 5′-terminal (lipo-ODN) was synthesized.
In vitro
and
in vivo
experiments were designed to compare the immune suppressive properties of lipo-ODN and unmodified ODN. Cellular uptake and distribution, inhibition of Toll-like receptor (TLR) activation, lymph nodes (LN) draining, and the suppression of antigen-specific immune responses in an ovalbumin protein model was investigated.
Results
Compared to unmodified ODN, lipid functionalized suppressive ODN demonstrated enhanced cellular uptake and TLR-9 specific immune suppression in TLR reporter cells. Additionally, injection of a low dose of lipid-modified suppressive ODN, but not the unconjugated ODN, accumulated in the draining LNs and exhibited potent inhibition of antigen-specific CD8
+
T cell and B cell responses
in vivo
.
Conclusions
Targeting suppressive ODN to antigen presenting cells (APCs) in the local LNs is an effective approach to amplify the immune modulation mediated by ODN containing repetitive TTAGGG motif. This approach might be broadly applicable to target molecular adjuvants to the key immune cells in the LNs draining from disease site, providing a simple strategy to improve the efficacy of many molecular immune modulators.
Obesity-related glomerulopathy (ORG) is characterized by glomerulomegaly with or without focal and segmental glomerulosclerosis lesions. Isothiocyanate sulforaphane (SFN) can protect kidneys from ...ORG-related damages. In this study, we investigated the effects of SFN as a preventive therapy or intervention for ORG to reveal its mechanism of action.
We established a mouse obesity model with preventive SFN or N-acetylcysteine treatment for 2 months. Thereafter, we used nuclear factor erythroid 2-related factor 2-deficient (Nrf2−/−) and wild type mice in our ORG model with SFN treatment. Finally, we generated a corresponding mouse podocyte model in vitro. The body weight, wet weight of perirenal-and peritesticular fat, and urinary albumin/creatinine ratio were assessed. We used periodic acid–Schiff staining and electron microscopy to assess the function of the kidneys and podocytes. In addition, we evaluated the expression of Nrf2 and podocyte-specific proteins by western blotting.
Treatment with SFN reduced body weight, organ-associated fat weight, and urinary albumin/creatinine ratio in both the preventive treatment and disease intervention regimens. SFN treated mice exhibited higher expression levels of podocyte-specific proteins and better podocyte function. However, treatment with SFN did not affect these parameters in obese Nrf2−/− mice. Light chain 3 of microtubule-associated protein 1-II and metallothionein had higher expression in the wild type than in the Nrf2−/− mice.
Treatment with SFN limited ORG-induced damage by enhancing podocyte autophagy via Nrf2.
•Sulforaphane suppresses ORG damage in preventive treatment and disease intervention regimens.•Nrf2−/− mice were not protected by sulforaphane from obesity-induced damage.•Sulforaphane enhanced autophagy in podocytes by regulating Nrf2 expression.
Design of selective sensors for a specific analyte in blood serum, which contains a large number of proteins, small molecules, and ions, is important in clinical diagnostics. While metal and ...polymeric nanoparticle conjugates have been used as sensors, small molecular assemblies have rarely been exploited for the selective sensing of a protein in blood serum. Herein we demonstrate how a nonspecific small molecular fluorescent dye can be empowered to form a selective protein sensor as illustrated with a thiol-sensitive near-IR squaraine (Sq) dye (λabs= 670 nm, λem= 700 nm). The dye self-assembles to form nonfluorescent nanoparticles (D h = 200 nm) which selectively respond to human serum albumin (HSA) in the presence of other thiol-containing molecules and proteins by triggering a green fluorescence. This selective response of the dye nanoparticles allowed detection and quantification of HSA in blood serum with a sensitivity limit of 3 nM. Notably, the Sq dye in solution state is nonselective and responds to any thiol-containing proteins and small molecules. The sensing mechanism involves HSA specific controlled disassembly of the Sq nanoparticles to the molecular dye by a noncovalent binding process and its subsequent reaction with the thiol moiety of the protein, triggering the green emission of a dormant fluorophore present in the dye. This study demonstrates the power of a self-assembled small molecular fluorophore for protein sensing and is a simple chemical tool for the clinical diagnosis of blood serum.