Anthropogenic substances pollute freshwater systems worldwide, with serious, long-lasting effects to aquatic biota. Present methods of detecting elevated levels of trace metal pollutants are ...typically accurate but expensive, and therefore not suitable for applications requiring high spatial resolution. Additionally, these methods are not efficient solutions for the determination of long-term averages of pollution concentration. This is the rationale for the implementation of a biomonitoring programme as an alternative means of pollutant detection.
This review summarises recent literature concerning the past and potential uses of the benthic isopod Asellus aquaticus as a biomonitor for pollution in freshwater systems. Recent studies indicate that A. aquaticus is well suited for this purpose. However, the mechanisms by which it bioaccumulates toxins have yet to be fully understood.
In particular, the interactions between coexisting trace metal pollutants in the aquatic environment have only recently been considered, and it remains unclear how a biomonitoring programme should adapt to the effects of these interactions. It is evident that failing to account for these additional stressors will result in an ineffective biomonitoring programme; for this reason, a comprehensive understanding of the bioaccumulation mechanisms is required in order to reliably anticipate the effects of any interferences on the outcome.
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•Asellus aquaticus is known to bioaccumulate trace metals.•A. aquaticus has been suggested as a potential biomonitor for metal pollutants.•A biomonitoring programme could act as an early warning system for pollution events.•Metal uptake and sequestering mechanisms are still poorly understood.•Antagonistic effects of metals must be better understood for implementation to succeed.
Capsule: “The benthic isopod, Asellus aquaticus, may be an ideal biomonitor for freshwater pollution.”
Abstract
Aggregation (gathering together) and sheltering (hiding in cover) are basic behaviours that can reduce the risk of predation. However, both behaviours have costs, such as increased ...competition over resources and high prevalence of contact-spread parasites (aggregation) or lost opportunities for foraging and mating (sheltering). Therefore, variation in these behaviours is expected between populations with varying levels of predation risk. We compared aggregation and sheltering in surface- (various predators) and cave-adapted (no predator) populations of the isopod Asellus aquaticus in a common garden experiment. Given that the cave environment is constantly dark, we also tested for population variation in light-induced behavioural plasticity. Variation in sheltering was explained by habitat type: cave individuals sheltered less than surface individuals. We found high between-population variation in aggregation with or without shelters and their light-induced plasticity, which was not explained by habitat type. Cave individuals decreased (habituation) whereas surface individuals increased sheltering with time (sensitization). We suggest that population variation in sheltering is driven by predation, whereas variation in aggregation must be driven by other, unaccounted environmental factors, in a similar manner to light-induced behavioural plasticity. Based on habituation/sensitization patterns, we suggest that predation-adapted populations are more sensitive to disturbance related to routine laboratory procedures.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
•Nutrional value of finished water determines invertebrate biomass.•All DWDS invertebrates occur in a wide variety of natural habitats.•Invertebrates in DWDS are preadapted to this oligotrophic ...environment.•Aeromonas/HPC in DWDS significantly correlated with invertebrate biomass.
Invertebrates such as Asellus aquaticus, halacarid mites, copepods and cladocerans are known to regularly occur in drinking water distribution systems (DWDS). An eight-year study investigated the biomass and taxonomic composition of invertebrates in the finished water of nine Dutch drinking water treatment plants (using surface water, ground water or dune-infiltrated water) and their non-chlorinated distribution systems. The main aims of the study were to examine the source waters’ influence on invertebrate biomass and composition in the distribution networks and to describe invertebrate ecology in relation to the habitat of filters and the DWDS.
Invertebrate biomass of the finished drinking waters of the surface water treatment plants was significantly higher than in the finished waters of the other treatment plants. This difference was due to the higher nutrient levels of the source water. The main part of the biomass in the finished water of the treatment plants consisted of rotifers, harpacticoid copepods, copepod larvae, cladocerans and oligochaetes, which are small-sized, euryoecious and tolerate broad environmental conditions. Most of them reproduce asexually.
Most species found in the DWDS are known to be detritivores, but all are benthic and euryoecious organisms, many of which have a cosmopolitan distribution. The euryoeciousness of these freshwater species was also shown by their occurrence in brackish waters and ground or hyporheic waters and the ability of many eurythermic species to overwinter in the DWDS habitat. These species are preadapted to the oligotrophic environment of the DWDS and can develop stable populations there. Most species can reproduce asexually and the sexually reproducing invertebrates (Asellus aquaticus, cyclopoids and probably also halacarids) have obviously overcome the potential problem of finding a mating partner. This study also showed a significant correlation of DOC in the drinking water with the invertebrate biomass. A. aquaticus was the dominant biomass component in six out of nine locations and was highly correlated with the Aeromonas counts in the DWDS. Thus, invertebrate monitoring in DWDS is an important additional parameter in understanding biological stability conditions in non-chlorinated DWDS.
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Effects of long-term, environmentally relevant doses of radiation on biota remain unclear due to a lack of studies following chronic exposure in contaminated environments. The 1986 Chernobyl accident ...dispersed vast amounts of radioactivity into the environment which persists to date. Despite three decades of research, impacts of the incident on non-human organisms continues to be contested within the scientific literature. The present study assessed the impact of chronic radiation exposure from Chernobyl on the developmental stability of the model aquatic isopod, Asellus aquaticus using fluctuating asymmetry (FA) as an indicator. Fluctuating asymmetry, defined as random deviations from the expected perfect bilateral symmetry of an organism, has gained prominence as an indicator of developmental stability in ecotoxicology. Organisms were collected from six lakes along a gradient of radionuclide contamination in Belarus and the Ukraine. Calculated total dose rates ranged from 0.06–27.1μGy/h. Fluctuating asymmetry was assessed in four meristic and one metrical trait. Significant differences in levels of pooled asymmetry were recorded between sample sites independent of sex and specific trait measured. However, there was no correlation of asymmetry with radiation doses, suggesting that differences in asymmetry were not attributed to radionuclide contamination and were driven by elevated asymmetry at a single site. No correlation between FA and measured environmental parameters suggested a biotic factor driving observed FA differences. This study appears to be the first to record no evident increase in developmental stability of biota from the Chernobyl region. These findings will aid in understanding the response of organisms to chronic pollutant exposure and the long term effects of large scale nuclear incidents such as Chernobyl and Fukushima.
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•30year impacts of Chernobyl on development of Asellus aquaticus assessed•Fluctuating asymmetry (FA) used as measure of developmental stability•No increase in developmental stability along gradient of radioactive contamination•Findings suggest resilience of aquatic invertebrate populations to radionuclides.•Helps to understand the impacts of chronic exposures to radiation on ecosystems
The objective of the present experimental study was to gain a better understanding of the foraging activity of Asellus aquaticus during fish egg incubation. A. aquaticus were introduced into ...experimental setups of dead eggs, viable eggs and hatched larvae of zebrafish (Danio rerio), a commonly used model organism. The amount of A. aquaticus and the duration of their exposure to the eggs significantly affected the proportion of consumed dead eggs in each experimental cycle. A. aquaticus belongs to the group of aquatic detritivores, and no predatory behavior was observed during the experiments. These crustaceans could distinguish between the dead eggs and those containing living embryos. Furthermore, zebrafish larvae remained unharmed by A. aquaticus, even in the absence of alternative food source. These findings underscore the potential sanitary role of these crustaceans in natural waters and offer new perspectives on their possible use as biological control organisms in aquaculture hatcheries. Additionally, our results suggest a potential application of A. aquaticus in combating pathogens by reducing the growth substrates for bacteria and fungi.
Many lakes in the northern hemisphere are browning due to increasing concentrations of terrestrial dissolved organic carbon (DOC). The consequences of lake browning to littoral invertebrates, ...however, are not fully understood. We analyzed community structure and fatty acid (FA) profiles of littoral invertebrates in humic (DOC-rich) and clear-water lakes in Eastern Finland. We found higher abundance of chironomids (Diptera: Chironomidae) in humic compared to clear-water lakes, whereas stoneflies (Plecoptera) and mayflies (Ephemeroptera: Baetidae) were more abundant in clear-water lakes. Taxon explained 65% of the differences in the FA composition of littoral invertebrates. However, the proportion and content of polyunsaturated FAs of several taxa were significantly higher in clear-water lakes compared to humic lakes. Our results reveal differences in both community structure and nutritional quality of littoral invertebrates for fish between humic and clear-water lakes.
Temperature is an important environmental factor that influences key traits like body size, growth rate and maturity. Ectotherms reared under high temperatures usually show faster growth, but reach a ...smaller final size, a phenomenon known as the temperature-size rule (TSR). Oxygen may become a limiting resource at high temperatures, when demand for oxygen is high, especially in water as oxygen uptake is far more challenging under water than in air. Therefore, in aquatic ectotherms, the TSR might very well be mediated by temperature effects on oxygen availability and oxygen demand. To distinguish between the direct effects of temperature and oxygen mediated effects, growth rate and final size were measured in the aquatic ectotherm Asellus aquaticus (Linnaeus, 1758) reared under different temperature and oxygen conditions in a factorial design. Growth could be best described by a modified Von Bertalanffy growth function. Both temperature and oxygen affected age at maturity and growth. Growth responses to temperature were dependent on oxygen conditions (interactive effect of temperature and oxygen). Only under hypoxic conditions, when oxygen was most limiting, did we find a classic TSR. Moreover, when comparing treatments differing in temperature, but where the balance between oxygen demand and supply was similar, high temperature increased both growth rate and final size. Thus effects of oxygen may resolve the life-history puzzle of the TSR in aquatic ectotherms.
•We examined the possible role of oxygen in creating the temperature-size rule.•Temperature and oxygen together explained length of A. aquaticus in a rearing study.•Oxygen limitation seemed important for generating the temperature-size rule.•Temperature and oxygen affected age at maturity.•Equalizing the ratio oxygen demand to supply may resolve the TSR puzzle.
We conducted a molecular survey on microsporidian diversity in different lineages (operational taxonomic units = OTUs) of Asellus aquaticus from 30 sites throughout Europe. Host body length was ...determined, and DNA was extracted from host tissue excluding the intestine and amplified by microsporidian-specific primers. In total, 247 A. aquaticus specimens were analysed from which 26.7% were PCR-positive for microsporidians, with significantly more infections in larger individuals. Prevalence ranged between 10 and 90%. At 9 sites, no microsporidians were detected. A significant relationship was found between the frequency of infected individuals and habitat type, as well as host OTU. The lowest proportion of infected individuals was detected in spring-habitats (8.7%, n = 46) and the highest in ponds (37.7%, n = 53). Proportion of infected individuals among host OTUs A, D and J was 31.7, 21.7 and 32.1%, respectively. No infections were detected in OTU F. Our results are, however, accompanied by a partially low sample size, as only a minimum of 5 individuals was available at a few locations. Overall, 17 different microsporidian molecular taxonomic units (MICMOTUs) were distinguished with 5 abundant isolates (found in 4–17 host individuals) while the remaining 12 MICMOTUs were “rare” and found only in 1–3 host individuals. No obvious spatio-genetic pattern could be observed. The MICMOTUs predominantly belonged to Nosematida and Enterocytozoonida. The present study shows that microsporidians in A. aquaticus are abundant and diverse but do not show obvious patterns related to host genetic lineages or geography.
Organisms well suited for the study of ecotype formation have wide distribution ranges, where they adapt to multiple drastically different habitats repeatedly over space and time. Here we study such ...ecotypes in a Crustacean model, Asellus aquaticus, a commonly occurring isopod found in freshwater habitats as diverse as streams, caves and lakes. Previous studies focusing on cave vs. surface ecotypes have attributed depigmentation, eye loss and prolonged antennae to several south European cave systems. Likewise, surveys across multiple Swedish lakes have identified the presence of dark‐pigmented "reed" and light‐pigmented "stonewort" ecotypes, which can be found within the same lake. In this study, we sequenced the first draft genome of A. aquaticus, and subsequently use this to map reads and call variants in surface stream, cave and two lake ecotypes. In addition, the draft genome was combined with a RADseq approach to perform a quantitative trait locus (QTL) mapping study using a laboratory bred F2 and F4 cave × surface intercross. We identified genomic regions associated with body pigmentation, antennae length and body size. Furthermore, we compared genome‐wide differentiation between natural populations and found several genes potentially associated with these habitats. The assessment of the cave QTL regions in the light–dark comparison of lake populations suggests that the regions associated with cave adaptation are also involved with genomic differentiation in the lake ecotypes. These demonstrate how troglomorphic adaptations can be used as a model for related ecotype formation.
Biodiversity loss is occurring rapidly worldwide, yet it is uncertain whether few or many species are required to sustain ecosystem functioning in the face of environmental change. The importance of ...biodiversity might be enhanced when multiple ecosystem processes (termed multifunctionality) and environmental contexts are considered, yet no studies have quantified this explicitly to date. We measured five key processes and their combined multifunctionality at three temperatures (5, 10 and 15 °C) in freshwater aquaria containing different animal assemblages (1–4 benthic macroinvertebrate species). For single processes, biodiversity effects were weak and were best predicted by additive‐based models, i.e. polyculture performances represented the sum of their monoculture parts. There were, however, significant effects of biodiversity on multifunctionality at the low and the high (but not the intermediate) temperature. Variation in the contribution of species to processes across temperatures meant that greater biodiversity was required to sustain multifunctionality across different temperatures than was the case for single processes. This suggests that previous studies might have underestimated the importance of biodiversity in sustaining ecosystem functioning in a changing environment.
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