Polyamine derivatives have a promising prospect in dealing with disseminated tumor cells, a major obstacle in cancer therapy. To develop a bifunctional polyamine derivative that can serve as a ...fluorescent probe and an antimetastatic agent, three kinds of polyamine conjugates with benzocdindol-2(1H)-one as a scaffold were designed and synthesized. Compound 5e was selected as a lead by in vitro screening. Two animal models demonstrated that 5e inhibited pulmonary metastasis and tumor growth. As a fluorescent probe, 5e might partially enter cells via a polyamine transporter and subsequently localize in the lysosome. Mechanistic investigations demonstrated the interdependence of 5e-triggered apoptosis and autophagy. Compound 5e modulated the expression of LC3-II, p62, cathepsins, and the expression of capases 3, caspase 8, Bcl-2, and p53. The SSAT-mediated Akt/β-catenin pathways were also inhibited by 5e. The dual features of 5e make it a worthwhile lead compound for further structural optimization.
This paper reviews the best known techniques using circular dichroism spectroscopy such as conventional circular dichroism (i.e. electronic circular dichroism), magnetic circular dichroisms (magnetic ...vibrational circular dichroism, x‐ray magnetic circular dichroism), fluorescence detected circular dichroism, near‐infrared circular dichroism, vibrational circular dichroism, Fourier transform infrared circular dichroism, high pressure liquid chromatography circular dichroism, stopped‐flow circular dichroism, and synchrotron radiation circular dichroism. Also, we have described here the most important applications of circular dichroism spectroscopy in structural biochemistry and nanoscience.
Silicon spraying on leaves can reduce the accumulation of cadmium (Cd) in rice grain. However, it has been found that not all rice varieties decrease in Cd content after silicon (Si) application. A ...field study was conducted to check the performance of Si on the accumulation and transport of Cd in four rice varieties. TY390 and YXY2, having 51.5%− 60.6% Cd content of grain was inhibited by foliar Si, were classified as CRS varieties; BXY9978 and YXYLS, having Cd content of grain is nonresponsive with Si, were classified as CNS varieties. The Cd contents were mainly accumulated in stem, especially in the first stem node. While foliar Si reported no changes in the Cd content of first node in four different rice varieties. Comparing the correlation between Si and Cd contents in the above part of the first internode of CRS and CNS, as well as the relative expression of Cd transport genes in the first internode suggested that first internode was the key site to effect Cd transport through Si application, and OsZIP7 is a key Cd transporter protein responsive to Si, leading to different response of Cd transport and accmulation between the CRS and the CNS varieties of rice.
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•Response of different rice varieties to Si mainly lies in the first internode.•OsZIP7 is the key Cd transporter responding to Silicon.•+Si treatment regulates genes of Cd transporter protein in inhibited varieties.•Application of Si reduced the transport of Cd to grain of inhibited varieties.•+Si has a positive effect on the Si transporter in the inhabited varieties.
Pot experiments were conducted to investigate the impacts of continuous addition of different concentrations of calcium chloride (CaCl2) and/or low-molecular-weight organic acids (LMWOAs) on soil pH, ...electrical conductivity (EC), and cadmium (Cd) transformation. These factors subsequently affected Cd phytoavailability in a system consisting of Cd-contaminated soil and Chinese cabbage (Brassica chinensis L.). The results indicate that CaCl2 addition had a greater impact on reducing soil pH value, increasing soil EC value, and enhancing Cd phytoaccumulation in Chinese cabbage compared to LMWOAs. When soil pH dropped by 0.3 unit and the soil EC increased by 500 µS cm−1, the Cd concentration in the Chinese cabbage shoots was 3 times higher than that in the control group. Throughout two planting terms of Chinese cabbage, the addition of CaCl2 (1.6–3.2 g kg−1) and LMWOAs (≤ 1.0 g kg−1) led to phytoextracted Cd concentration exceeding exchangeable Cd concentration in soil samples before the pot experiment. Regarding phytoextracted Cd, desorption from carbonate-bound Cd contributes more than desorption from bound to organic matter Cd and adsorption to Fe/Mn oxide Cd. This study underscores the influence of soil pH and EC value variations and Cd transformation on Cd phytoavailability. Special attention should be given to leafy vegetables grown in Cd-contaminated soil, as the phytoavailable Cd concentration reaches approximately 2.0 µg kg−1, which may lead to Cd levels surpassing acceptable limits for Chinese cabbage.
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•Soil pH fell by 0.3 and EC rose by 0.5 dS m− 1 causing 3 times of CCd in Chinese cabbage.•Cd transformation between Cd fractions affects the phytoextracted Cd from soil.•Phytoavailable of CCd at 2.0 µg kg− 1 in soil may lead to 0.2 mg kg− 1 of CCd in Chinese cabbage.•Soil Cd threshold for food safety should be re-established based on phytoavailable Cd.
ABSTRACT
We investigate the internal structure of elliptical galaxies at z ∼ 0.2 from a joint lensing–dynamics analysis. We model Hubble Space Telescope images of a sample of 23 galaxy–galaxy lenses ...selected from the Sloan Lens ACS (SLACS) survey. Whereas the original SLACS analysis estimated the logarithmic slopes by combining the kinematics with the imaging data, we estimate the logarithmic slopes only from the imaging data. We find that the distribution of the lensing-only logarithmic slopes has a median 2.08c ± 0.03 and intrinsic scatter 0.13 ± 0.02, consistent with the original SLACS analysis. We combine the lensing constraints with the stellar kinematics and weak lensing measurements, and constrain the amount of adiabatic contraction in the dark matter (DM) haloes. We find that the DM haloes are well described by a standard Navarro–Frenk–White halo with no contraction on average for both of a constant stellar mass-to-light ratio (M/L) model and a stellar M/L gradient model. For the M/L gradient model, we find that most galaxies are consistent with no M/L gradient. Comparison of our inferred stellar masses with those obtained from the stellar population synthesis method supports a heavy initial mass function (IMF) such as the Salpeter IMF. We discuss our results in the context of previous observations and simulations, and argue that our result is consistent with a scenario in which active galactic nucleus feedback counteracts the baryonic-cooling-driven contraction in the DM haloes.
Cadmium (Cd), which seriously affects plant growth and crop production, is harmful to humans. Previous studies revealed ryegrass (Lolium multiflorum Lam.) exhibits Cd tolerance, and may be useful as ...a potential hyperaccumulator because of its wide distribution. In this study, the physiological and transcriptional responses of two ryegrass cultivars i.e., high (LmHC) and low (LmLC) Cd tolerance to Cd stress were investigated and compared. The Cd tolerance of LmHC was greater than that of LmLC at various Cd concentrations. The uptake of Evans blue dye revealed that Cd-induced root cell mortality was higher in LmLC than in LmHC after a 12-h Cd treatment. Furthermore, the content and influx rate of Cd in LmLC roots were greater than in LmHC roots under Cd stress conditions. The RNA sequencing and quantitative real-time PCR data indicated that the Cd transport regulatory genes (ABCG37, ABCB4, NRAMP4, and HMA5) were differentially expressed between the LmLC and LmHC roots. This expression-level diversity may contribute to the differences in the Cd accumulation and translocation between LmLC and LmHC. These findings may help clarify the physiological and molecular mechanisms underlying ryegrass responses to Cd toxicity. Additionally, ryegrass may be able to hyperaccumulate toxic heavy metals during the phytoremediation of contaminated soil.
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•The Cd tolerance of LmHC was stronger than LmLC.•The influx rate and accumulation of Cd in the LmLC roots were greater than LmHC.•Up-regulated expression of ABCG37, ABCB4, and ABCB21 might improve Cd uptake.•LmLC is a potential hyperaccumulator for bioremediation of soil contaminated with Cd.
Atmospheric deposition of heavy metals such as Cd is a threat to ecosystems and food safety. Our knowledge is still limited about the effectiveness of remediation process for Cd-contaminated ...agro-soils under atmospheric Cd deposition. In this study, eight soil amendments were used in a Cd-contaminated purple soil to investigate their impacts on soil Cd availability, microbial response, and Cd uptake by mustard and corn plants via simulating the atmospheric Cd deposition under laboratory incubation and greenhouse conditions. Results showed that the simulated atmospheric Cd deposition increased the soil high-risk Cd (HR, exchangeable and carbonate Cd) and decreased soil medium-risk Cd fraction (MR, bound to Fe/Mn oxide and organic Cd), and the largest direct effects on crop Cd uptakes were 0.94 and 0.66 for mustard and corn based on the path-coefficient analysis, respectively. Generally, Cd deposition led to decreasing soil microbial biomass carbon, populations of bacteria, fungi and actinomycetes, and enzyme activities of urease, catalase, sucrase, and acid phosphatase whereas increasing soil microbial biomass nitrogen. Compared with control and lime treatments, an organic-inorganic combined preparation (OCP) appeared to be effective for remediation of the Cd-contaminated purple soil due to its potential to increase the HR-Cd and reduce both MR-Cd and crop Cd uptake, as accompanied by its neutral effects on soil bacterial alpha diversity and community structure. Results also indicated that application of nitrogen fertilizers should be considered for remediation of the Cd-contaminated soils as nitrogen inputs were demonstrated to promote soil health under elevated Cd condition.
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•Atmospheric Cd deposition was explored firstly in Cd-contaminated soil remediation.•Direct effects of deposited Cd on Cd uptakes of mustard and corn were quantified.•Optimum amendment was made for Cd-contaminated purple soil exposed to Cd deposition.•Different responses of soil microbiome to simulated Cd deposition.•Nitrogen fertilizer should be considered during the Cd-contaminated soil remediation.
CD68/macrosialin: not just a histochemical marker Chistiakov, Dimitry A; Killingsworth, Murry C; Myasoedova, Veronika A ...
Laboratory investigation,
January 2017, 2017-01-00, 20170101, Letnik:
97, Številka:
1
Journal Article
Recenzirano
Odprti dostop
CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Traditionally, CD68 is exploited as a valuable cytochemical marker to immunostain ...monocyte/macrophages in the histochemical analysis of inflamed tissues, tumor tissues, and other immunohistopathological applications. CD68 alone or in combination with other cell markers of tumor-associated macrophages showed a good predictive value as a prognostic marker of survival in cancer patients. Lowression of CD68 was found in the lymphoid cells, non-hematopoietic cells (fibroblasts, endothelial cells, etc), and tumor cells. Cell-specific CD68 expression and differentiated expression levels are determined by the complex interplay between transcription factors, regulatory transcriptional elements, and epigenetic factors. Human CD68 and its mouse ortholog macrosialin belong to the family of LAMP proteins located in the lysosomal membrane and share many structural similarities such as the presence of the LAMP-like domain. Except for a second LAMP-like domain present in LAMPs, CD68/microsialin has a highly glycosylated mucin-like domain involved in ligand binding. CD68 has been shown to bind oxLDL, phosphatidylserine, apoptotic cells and serve as a receptor for malaria sporozoite in liver infection. CD68 is mainly located in the endosomal/lysosomal compartment but can rapidly shuttle to the cell surface. However, the role of CD68 as a scavenger receptor remains to be confirmed. It seems that CD68 is not involved in binding bacterial/viral pathogens, innate, inflammatory or humoral immune responses, although it may potentially be involved in antigen processing/presentation. CD68 could be functionally important in osteoclasts since its deletion leads to reduced bone resorption capacity. The role of CD68 in atherosclerosis is contradictory.