Introduction: Hedera helix is an evergreen plant used in treatment of wounds in the Iranian folk medicine. One of the main stages in wound healing process is, the proliferative phase in which ...fibroblasts proliferate within the wounds and produce extracellular matrix. Collagen is the main constituent of extracellular matrix. So the aim of this study was to evaluate the effect of Hedera helix alcoholic extract on dermal collagen bundles in wound healing process. Methods: 7 adult Oryctolagus Caniculus rabbits were enrolled in this experiment. Four wounds were created on each rabbit. Alcoholic extract of hedera helix dried leaves were prepared. Wounds were treated with eucerin, phenytoin, hedera helix 10% and 20% eucerin-based creams for 20 days. On day 21 (after the completion of the experiment), skin samples were taken and the collagen bundles in reticular and papillary layers of the dermis were evaluated histologically. Results: Histological assessment of dermal collagen bundles in the reticular layer showed that both 10% and 20% hedera helix creams, increased collagen bundle thickness significantly, compared to eucerin treated wounds (P<0.001 and P<0.01). In papillary layer there was no significant effect in both groups. Compared to eucerin treated wounds, collagen bundles were more organized in phenytoin and hedera helix 10% and 20% cream treated wounds, in a Histomorphological point of view. Conclusion: Our results showed that hedera helix contains phytochemicals which may act on the proliferative phase of wound healing process.
Display omitted
•Untargeted HPTLC-image analyses chemical profiling applied to classify Hedera helix subspecies.•PCA revealed separation between samples using data set of phenolics ...profiling.•Imortnat variables were identified using HPTLC/MS.•HPTLC method developed for quantification of six phenolics and three saponins.•OPLS model used to screen bioactive markers in samples using antimicrobial data.
A strategy combining untargeted and targeted chemical profiling for discrimination of the different subspecies of Hedera helix L (Ivy) based on HPTLC fingerprints and antioxidant and antimicrobial activity testing followed by chemometric analysis has been implemented for the first time for the holistic profiling of bioactive secondary metabolites in the different subspecies. Principal component analysis (PCA) based on HPTLC-image analysis fingerprints of secondary metabolites loading plots were utilized for determination of chemical markers responsible for the classification which were then identified using HPTLC/MS technique as the six phenolics; caffeic acid, kaempferol-3-O-glucoside, quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, rutin and chlorogenic acid and the three saponins; alpha-hederin, hederasaponin B and hederacoside C. The identified markers were then quantified using a HPTLC-high resolution plate imaging technique for targeted profiling. Biomarkers were determined from the coefficients plot of orthogonal projection to latent structures (OPLS) model based on the secondary metabolites quantitative data together with the biological activity data which revealed that the phenolic metabolites were strongly correlated to the antioxidant and antimicrobial activity data points compared to saponins. According to targeted hierarchical clustering analysis (HCA)-heat-map, samples could be divided into three main clusters; H. helix subsp. helix cluster, H. helix subsp. rhizomatifera and H. helix subsp. hiberinica sub-clusters and H. helix subsp. poetrum and H. helix subsp. canariensis sub-clusters, and such clustering pattern was directly related to the variation in the content of bioactive phenolics among the different subspecies rather than their saponins content, providing an insight into the unsettled taxonomy of the ivy subspecies and successful precise efficacy-directed discrimination of the different samples.
Patients with complaints of coughing make up a significant percentage of doctors in various specialties. Cough is not an independent disease, in most clinical cases it is a symptom of a respiratory ...disease. In acute respiratory pathology, the cough is usually short-lived and stops after the eradication of the infectious agent and the relief of inflammatory changes. It is important therapeutically to provide a reduction in the duration and relief of cough, as well as the prevention of complications. An effective symptomatic agent in the treatment of coughs of various origins, including inflammatory diseases of the respiratory tract, are herbal preparations. The fairly widespread use of phytopreparations in clinical practice is due to their high safety, physiological action, availability and effectiveness. The pharmacological action of these drugs in respiratory infections is manifested by antiinflammatory, cytoprotective, antiviral and antioxidant effects, multifactorial improvement of mucociliary cleansing processes. One of these phytopreparations is a monocomponent syrup with expectorant, mucolytic and antispasmodic. The therapeutic effect of drug is explained by the presence in the composition of triterpene glycosides (saponins) from the leaves of Hedera helix . The effectiveness and safety of drug is proved by a sufficient volume of clinical studies and observations. Literature data show that Hedera helix leaf preparations significantly reduce the severity of acute respiratory infections of the upper respiratory tract.
Virtually none of the patients had severe side effects. Allergic reactions were recorded in less than 0.5% of cases, with no mention of a severe allergic reaction of the anaphylaxis type. Thus, the drug of plant origin in the form of drops can be successfully used in patients with symptoms of acute respiratory diseases.
Purpose
Acute cough due to viral upper respiratory tract infections (URTIs) and bronchitis is a common reason for patients to seek medical care. Non-antibiotic over-the-counter cough medications such ...as ivy leaf extract are frequently used but their efficacy is uncertain. Our purpose was to update our previous systematic review and evaluate the effectiveness and tolerability of ivy leaf in the treatment of acute URTIs in adult and pediatric populations.
Methods
We searched MEDLINE, EMBASE, the Cochrane Library, and clinical trial registries from December 2009 to January 2020. Randomized controlled trials (RCTs), controlled clinical trials (CCTs), and observational studies (OSs) investigating ivy leaf mono- or combination preparations were included. Two independent reviewers assessed records for eligibility and risk of bias and performed data extraction.
Results
Six RCTs, 1 CCT, and 4 OSs were identified. Since the publication of our previous review, the number of RCTs has increased. All studies concluded that ivy leaf extract is an effective and safe option for the treatment of cough due to URTIs and bronchitis. Three RCTs reported a more rapid reduction in cough severity and/or frequency under ivy leaf treatment. The clinical significance of these effects appears to be minimal. No serious adverse effects were reported. The overall quality of reporting was low and the risk of bias was high.
Conclusions
Ivy leaf preparations are safe for use in cough due to acute URTIs and bronchitis. However, effects are minimal at best and of uncertain clinical importance.
Bee pollen, known as a 'life-giving dust', is a product of honeybees using flower pollen grains and combining them with their saliva secretions. Thus, flower pollen could be an indicator of the bee ...pollen botanical source. Identification of bee pollen sources is a highly crucial process for the evaluation of its health benefits, as chemical composition is directly related to its pharmacological activity. In this study, the chemical profiles, contents of phenolic marker compounds and pharmacological activities of
L. (ivy) bee pollen samples from Türkiye and Slovenia, as well as ivy flower pollen grains, were compared. High-performance thin-layer chromatography (HPTLC) analyses revealed that pollen samples, regardless of where they were collected, have similar chemical profiles due to the fact that they have the same botanical origins. Marker compounds afzelin, platanoside and quercetin-3-
-β-glucopyranosyl-(1→2)-β-galactopyranoside, common to both bee pollen and flower pollen, were isolated from bee pollen, and their structures were elucidated by nuclear magnetic resonance (NMR) and mass spectrometry (MS). These three compounds, as well as chlorogenic acid and 3,5-dicaffeoylquinic acid (found in flower pollen), were quantified using high-performance liquid chromatography (HPLC) analyses.
tests and effect-directed analyses were used to evaluate the xanthine oxidase inhibition and antioxidant activity of the marker compounds and extracts from flower pollen and bee pollen. This is the first report comparing chemical profiles and related bioactivities of the flower pollen and bee pollen of the same botanical origin, as well as the first report of the chemical profile and related bioactivities of ivy flower pollen.
Моніторинг динаміки популяцій має велике теоретичне й практичне значення. Найбільший інтерес представляють популяції рідкісних видів або тих, які перебувають на межі ареалу. Такі види діагностують ...добре інтегровані комплексні екологічні характеристики. Вони є чутливим інструментом для моніторингу стану навколишнього середовища. Природний ареал Hedera helix уключає територію Західної Європи та південно-західну частину Східної Європи. Вид довгий час уважався реліктовим. Г. К. Смик пояснює диз’юнкції ареалу Hedera helix наслідком масової вирубки лісів у 20-х роках минулого століття. Нові знахідки виду на території Полісся ставлять під сумнів ці гіпотези. Нами проаналізовано середовище існування виду, флору та рослинність у межах місцезнаходжень на території Словечансько-Овруцького кряжу. Ми використовуємо цей регіон як модельний через надзвичайно високу різноманітність оселищ і нові знахідки локалітетів Hedera helix. Ландшафтна й видова різноманітність кряжу вища, ніж на будь-якій іншій території Полісся. Матеріалами дослідження стали стандартні геоботанічні описи. Дослідження тривало з 2004 по 2018 р. Для оцінки середовища використовували метод синфітоіндикації. Антропогенну оцінку здійснювали за допомогою визначення гемеробії (антропотолерантності) угруповань за шкалою Дідуха-Хом’яка. Синфітоіндикаційний аналіз проводили з використанням програми Simargl 1.12. Наші дослідження показали, що вид присутній в екотопах із вузькою амплітудою показників екологічних факторів. Hedera helix на території Словечансько-Овруцького кряжу трапляється в умовах стенотипних умов (перекриття 3 % синфітоіндикаційної шкали) в екосистемах з автотрофним блоком у вигляді асоціації Stellario holosteae-Carpinetum betuli (клас Carpino-Fagetea). Однак поруч наявні території, які мають подібні екологічні умови, але чомусь не зайняті Hedera helix. Пошук відповіді на це питання потребує більш глибоких досліджень.
Indoor potted plants are able to remove volatile organic compounds (VOC) from air, but only few studies have investigated the removal of compounds in mixtures. Here, we present a non-targeted ...pixel-based fingerprinting analysis documenting the removal of a complex mixture of gasoline VOCs by Hedera helix under dynamic chamber conditions allowing for air exchange and continuous gasoline exposure. For 15 days, the entire potted plant was exposed to gasoline; subsequently, the epigeous plant parts were removed and the soil microcosm (i.e. soil, plant roots and microorganisms) was exposed to gasoline for another eight days. Quantitative analysis was performed for heptane, 3-methylhexane, toluene, ethylbenzene and m,p-xylenes, and the CHEMSIC method (CHEMometric analysis of Selected Ion Chromatograms) was used for non-targeted pixel-based fingerprinting analysis. The quantitative analysis demonstrated that the presence of potted plants or pots without epigeous plant parts led to a reduction of selected VOCs by 16.7–22.6%. The CHEMSIC method confirmed this and revealed that all gasoline VOCs were reduced in concentration when H. helix was present. The estimate for the total VOC removal was in the range of 11–32%. The removal was highest for samples where the epigeous plant parts were absent and compounds known to be hard to degrade by microorganisms such as dimethylcyclopentanes were removed the least compared to compounds more easily degraded by microorganisms such as heptane when epigeous plant parts were removed. All findings support the conclusion that the soil microcosm was the main responsible for the removal of VOCs.
•Potted Hedera helix was exposed to gasoline under dynamic conditions.•Data was analyzed by a non-targeted pixel-based fingerprinting approach.•All gasoline VOCs were reduced in concentration by H. helix.•Preferential removal of some compounds was observed.•The soil microcosm play a substantial role in removal of gasoline VOCs.
In this paper, the extract from the leaves of Hedera helix L. was subjected to isolation and fractionation of saponins, in order to determine their in vitro antiproliferative activity. The ethanolic ...extract was obtained by maceration from H.helix L. leaves, grown in Romania, and the saponins were isolated and fractionated using column chromatography. The total saponin content in the extract and the isolated saponins was determined. Three saponins of interest, hederacoside C, α-hederin and hederagenin were identified by LC/MS analysis. Four selected fractions rich in these saponins tested in vitro, showed antiproliferative effects at a concentration of 200 µg/mL in a culture of human cervix carcinoma Hep-2 cells. The tested fractions were non-cytotoxic in the range of concentration 2–400 µg/mL on normal fibroblasts cells from NCTC cell line. Our results demonstrated the selected saponin fractions isolated from H.helix L. leaves may be considered as possible antitumor agents.
Display omitted
•The determination of saponin content in Hedera helix L. extracts was optimized.•Isolation, fractionation and identification of saponins was performed.•The samples cytotoxic effect was evaluated in vitro against normal fibroblasts NCTC cells and epithelial tumor Hep-2 cells.•Estimation of the antiproliferative activity of isolated and fractionated saponins was compared to that of standard saponins.
•In times of climate change with its diverse impacts on environment and urban buildings, the relevance of façade plants for the urban climate and air quality is being recognized as increasingly ...important. One of the main hurdles in the quantification of such ecosystem services is associated with the difficulty to obtain and design systems that provide detailed information on the interaction between various gases and the plant in question. This paper focuses on the detailed description of methods and processes to analyze the gas-absorptive behavior of especially façade relevant plants when exposed to gaseous pollutants.
How much do specific climbing plants contribute to the cleansing or absorption of harmful greenhouse and pollutant gases; often regarded as the main environmental threat in cities due to their adverse effects on human health? One of the main hurdles in the quantification of such ecosystem services is associated with the difficulty to obtain and design systems that provide detailed information on the interaction between various gases and the plant in question. To tackle these questions, two highly precise and accurate instruments, namely a mid-infrared laser absorption spectrometer (TDL) and a cavity-ring-down spectrometer (CRDS) were used to monitor the fate of gases when exposed to façade climbing plants like ivy. In a laboratory setting, a relaxation type of experiment was used consisting of a reaction chamber equipped with plant species and continuously flushed by synthetic air. This setup was used to determine the timescales of decay after short injections of the above-mentioned gases. After these injections, all gases followed simple exponential decay curves. N2O, a non-reactive (inert) tropospheric gas, was used as a reference to which all other gases were compared and thereby quantified. This paper focuses on the detailed description of methods and processes to analyse the gas-absorptive behaviour of plants when exposed to gaseous pollutants. For demonstration purposes, quantified absorption features of nitrogen oxide (NO2) are presented for ivy of the variety Hedera helix “Plattensee”. Results of this method of quantification showed that - as compared to N2O (control), - NO2 had a reduced residence time (time scale) of 100 s, while N2O resulted in a 600 s residence time (indicating no interference with the plant). This is equivalent to a 0.3 cm/s deposition velocity/ absorption rate of NO2 under light conditions.
Introducción y objetivos: Las prácticas de manejo de especies no-nativas e invasoras es tema prioritario en áreas protegidas, en especial en estadios tempranos de invasión, porque facilita la ...recuperación de sistemas nativos. Aquí presentamos una metodología para el control de una planta trepadora invasora, Hedera helix (Araliaceae).
M&M: Se realizó remoción manual y la disposición de los residuos se compostó en bolsas en el lugar. Luego se monitoreó la regeneración natural del bosque nativo. La propuesta incluye la convocatoria de voluntarios, enmarcados en un proyecto de investigación y extensión de la Universidad Nacional Comahue Bariloche con la colaboración de la Administración de Parques Nacionales.
Resultados: Desde 2019 participaron 47 voluntarios, se extrajeron 870 kg (peso húmedo) de material de cinco de los seis sitios identificados. Dos de los cinco sitios intervenidos se encuentran en la etapa de monitoreo de especies nativas.
Conclusión: La participación de voluntarios resultó efectiva en términos de las acciones de control manual, al estar los sitios controlados. De esta manera, se está logrando con éxito un mínimo impacto negativo sobre el entorno, al menos en las especies de plantas, evidenciado por una rápida recolonización pasiva de especies nativas y la eliminación la especie invasora en los sitios intervenidos. En particular, este modo de trabajo permite crear conciencia del problema de las invasiones biológicas en la comunidad y en futuros profesionales. También fortalece las relaciones entre instituciones vinculando la academia con la gestión en conservación, un aspecto a veces difícil de lograr.
PDF
