Sandy everlasting
(L.) Moench is herbaceous perennial plant belonging to Asteraceae family and it is native to Europe, Central Asia, and China. It belongs to the section HELICHRYSUM (Asteraceae ...family, genus
) along with
DC. Prodr., which very similar phenolic profile and
(Roth), which is widely used for essential oil extraction. Its flowers have a long tradition in European ethnomedicine as a cholagogue, choleretic, hepatoprotective, and detoxifying herbal drug. The flowers are rich in phenolic compounds including flavonoids, chalcones, phenolic acids, coumarins, and pyrones. Apart from polyphenols, other compounds such as sterols, lignans, and glycosides of aromatic compounds have been also isolated from
. The majority of authors confirm that the most important group of compounds responsible for biological activities is flavonoids. Moreover, significant activities of naringenin, one of the main flavonoids of
, were reported. On the other hand, there are no clinical data about testing the extracts or preparations based on
. Although
is well known in phytotherapy for its potential in the treatment of gallbladder disease and are classified as endangered species in a number of European countries, very few data about its cultivation are available in the literature.
The phytochemical analysis of the investigated Immortelle essential oil revealed the presence of monoterpenes and sesquiterpenes as major components that might be efficient as a wound healing ...potential agent. The present study aimed to develop an ointment based on the Immortelle essential oil and investigate its wound healing effects on excision wounds in diabetic rats. The topical formulated Immortelle ointment was subjected to pharmaco-technical characterization. Thirty-two diabetic rats with the induced excision wound were used to evaluate in vivo wound healing effects of ointment. The animals were randomly divided into four groups untreated or topically treated with either a 1% silver sulfadiazine, the ointment base, or Immortelle ointment. The response to the treatment was assessed by macroscopic, biochemical and histopathological analysis. The ointment, compatible with the skin remained stable for 6 months. Topical application of the Immortelle ointment showed the highest wound contraction with the highest content of hydroxyproline in comparison to the all examined groups. The Immortelle ointment showed significant wound contraction from day 7 to day 21 as compared to other groups. On the day 21, there was an average of 99.32% wound contraction in the Immortelle group, whereas the mean wound contraction in the negative control and ointment base group was 71.36% and 81.26% respectively. The histopathological results validated the potential wound healing effect of Immortelle ointment with evident post-excision scar maturation and increased collagen fibers density. Our findings revealed that the Immortelle ointment approach might serve as a promising and innovative tool for wound healing.
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•Immortelle ointment remained stable for 6 months.•The rheological behavior followed a non-Newtonian pseudoplastic pattern.•Immortelle ointment did not show any signs of dermal irritation.•Administration of Immortelle ointment increases wound contraction in rats.•Immortelle ointment accelerates wound healing by stimulating collagen deposition.
•Helichrysum italicum is used in pharmaceutical, cosmetic and perfume industry.•Significant antioxidant, anti-inflammatory, antimicrobial activities.•Chemotypes mainly rich either in oxygenated ...compounds or hydrocarbons.•Difficult distinction among subspecies due to high morphological variation.•Further research on the genetic diversity and agronomic management is essential.
Helichrysum italicum (Roth) G. Don is a thermophilus perennial from family Asteraceae. Due to versatile biological activities and applications, the interest in Helichrysum italicum scientific research and use has increased in the last couple of decades. Consequently, due to the increased industrial demand its plantation farming experienced a huge expansion in Eastern European Mediterranean countries. The aim of this review article was to present the available data on Helichrysum italicum and to identify the gaps in the knowledge, and openings for future potential investigations and contributions to the topic. The review of the Scopus database was performed and scientific studies from 1990 to the most recent publications in 2019 were summarized. The article includes the last reviewed taxonomy, detailed description of morphological characteristics, geographical distribution, uses, chemical diversity, biological activity (antimicrobial, antiviral, antioxidant, anti-inflammatory, repellent, and insecticidal activity), and genetic diversity. Additionally, some other potential uses are described. The majority of the available scientific data is focused on the chemical composition of the essential oil, however, little is known about the cultivation management of Helichrysum italicum that includes sowing requirements, seedling production, plant density, fertilization and nutrition requirements, harvesting, diseases, insects, and weed control. Genetic information on Helichrysum italicum is also scarce, but needed for future breeding programs of Helichrysum italicum and selection of new cultivars.
Since most studies regarding immortelle (Helichrysum italicum) are focused on the properties and composition of its essential oil, the aim of this study was to develop edible films based on ...immortelle water extract. Alginate and pectin combined with various proteins served as carriers for the formulation of biodegradable films and hydrogel particles. Films with different biopolymers and incorporated hydrogel particles were prepared by casting method and hydrogel particles were produced by ionic gelation. The bioactive profile (the content of total (TPC) and specific polyphenols, hydroxycinnamic acids (HCAC) and antioxidant capacity (AC)) of the developed matrices was characterized using spectrophotometric methods and high performance liquid chromatography (HPLC). Zeta-potential and rheological properties of alginate- and pectin-based film-forming solutions and physico-chemical (color, particle size, film thickness, dry matter content) and sensory properties of the developed particles and films were evaluated. The highest TPC (31.31 mg GAE/g sample) and HCAC (27.20 mg CAE/g sample) as well as the highest AC (0.15 mmol TE/g sample) was determined in pectin-based films. The addition of proteins decreased the content of the examined bioactive parameters, while the addition of hydrogel particles altered their physico-chemical properties. The obtained results indicate a great application potential of the developed biodegradable matrices.
•Immortelle extract is abundant in polyphenols, resulting in high bioactivity.•Chlorogenic acid derivatives dominate the bioactive profile of immortelle films.•The addition of hydrogels significantly enriched the bioactive profile of the films.•Polyphenols entrapped in films with hydrogels exhibit a prolonged release in SGF.•Alginate-based films have more desirable sensory properties than the pectin-ones.
The biofilm formation of bacteria in different parts of the human body can influence the success of antibiotic therapy. Essential oils (EOs) and their components are becoming increasingly popular in ...point of view of medicinal applications, because of their antibacterial efficacy. The immortelle EO has been used traditionally as an expectorant; however, there are no studies summarizing its antibacterial effect against respiratory tract bacteria. Our aim was to investigate the antibacterial and biofilm inhibitory activity of immortelle (Helichrysum italicum) EO against respiratory tract pathogens such as Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa and Streptococcus pneumoniae. In order to prove the antibacterial effect of the immortelle EO, broth microdilution and biofilm inhibition tests, and membrane damage assay were investigated. Scanning electron microscopy was used to identify the structural modifications in bacterial cells. Our results showed that immortelle EO has antibacterial and anti-biofilm effects against respiratory tract bacteria used in this study. H. parainfluenzae was the most sensitive to each treatment, however, P. aeruginosa was the most resistant bacteria. In conclusion, the studied EO may have a role in the treatment of respiratory tract infections due to their antibacterial and anti-biofilm activity.
Helichrysum italicum (immortelle), Asteraceae, is a perennial plant species with increasing market demand for its essential oil possessing anti-inflammatory, antibacterial, and antioxidant ...properties. The present study deals with clonal in vitro propagation of high-productive immortelle individuals. In vitro culture was initiated from stem tips on basal MS medium and sub-cultured several times on the same medium or on media supplemented with activated charcoal or with different plant growth regulators. Plants differed by their height, ramification, in vitro rooting, and callus formation. The highest propagation rate of 13.5±4.2 new shoots per explant, along with spontaneous rooting, was noticed in spring on the control MS medium. About 180 plants were successfully ex vitro adapted first in a phytotron, then in a greenhouse, and 14 were transferred outdoor in the ex situ collection where they acclimatized and bloomed in the first season. The nuclear DNA amount in 65 individuals was measured by flow cytometry, and no deviations in the ploidy level were detected. The results suggest the genome of the in vitro obtained plants is stable. Essential oil was extracted by water distillation from two pooled samples, one for the mother plants and another one for the ex vitro adapted plants. The main components identified by GC/MS in both samples were α-pinene, nerol acetate, and γ-curcumene. The results proved that the method of the in vitro clonal propagation is reliable for obtaining of numerous true-to-type plants in a short time, by direct organogenesis of high-productive individuals of H. italicum.
(Roth) G. Don., immortelle, is a plant species used in ethnomedicine and the food industry as a spice added to food, beverages, and bakery products. It has been shown to possess various biological ...activities, such as antioxidant and antibacterial activity, making it useful as a natural preservative. We investigated the phytochemical profile and biological activity of
essential oils from wild-grown plant material collected from natural habitats in the Republic of Croatia and Bosnia and Herzegovina. Using high-resolution scanning electron microscopy (SEM), a visual investigation of plant organs (stem, leaf, and flower) was performed, confirming the presence of essential oil reservoirs on the surface of all examined plant organs. Essential oils were isolated by hydrodistillation in the Clevenger apparatus. The chemical composition of the essential oils was determined using the GC-MS analytical technique. Cytotoxic activity tests were performed in vitro on three cell lines: skin (fibroblast), lung, and breast cancer. Using statistical tools, the synergistic and selective effects of
essential oil on healthy and tumor cells were correlated to chemical composition and cytotoxic activity. The synergistic and antagonistic effects of
essential oil's individual components were simulated by testing pure compounds and their mixture of cytotoxic activity on fibroblasts and breast cancer cells. The results confirm that essential oil's biological activity is much greater than the sum of the effects of its components. The present data are novel contributions to the body of knowledge on the biological activity of this species used in the food industry.
Immortelle (Helichrysum italicum) essential oil is one of the most valuable essential oils with its commercial importance and favorable characteristic properties in various cosmetic products and food ...products. Current research performed a comprehensive characterization of Immortelle essential oil using powerful and robust analytical techniques. The relative volatile composition of the Immortelle essential oil from Turkey was determined by GC-MS (gas chromatography-mass spectrometry) technique. Thirty-eight volatile compounds were identified. Average total phenolic content and DPPH radical scavenging activities (IC
50
) were determined as 54.24 mg GAE/mL essential oil and 0.27 mg/ml, respectively. FTIR and Raman spectroscopy fingerprints were determined for chemical characterization. The thermal profile was obtained by DSC (differential scanning calorimetry) analysis. The Immortelle essential oil (12.5 μg/mL) inhibited the growth of Caco 2 adenocarcinoma cells at the ratio of 48% without any deterioration in healthy L929 fibroblast cells according to the cytotoxicity analyses.
Lavender and immortelle essential oils (EOs) are widely used to treat a spectrum of human conditions. The aim of this study was to investigate cyto/genotoxic effects of lavender and immortelle EOs ...using plant cells (Allium cepa) and human lymphocytes, as well as their antimicrobial potential using nine strains of bacteria and fungi. Our results for lavender and immortelle EOs showed that the frequency of chromosome aberrations (CAs) was increased in comparison with controls. For both oils, increased frequency of apoptosis for all concentrations, as well as the frequency of necrosis (0.10/0.30 µl/ml for lavender/immortelle, respectively) was demonstrated. In human lymphocytes, differences for minute fragments between immortelle oil (0.10 µl/ml) and controls were observed. Increased frequency of apoptosis was detected for immortelle oil (0.20 µl/ml), while both oils (0.20; 0.30 µl/ml lavender, and immortelle at all concentrations) induced higher frequency of necrosis in comparison with controls. Lavender EO was effective against all tested Gram-positive and Gram-negative bacteria, while immortelle EO inhibited only Gram-positive bacteria. Both oils exhibited antifungal effect. Our results demonstrated that lavender and immortelle EOs showed cyto/genotoxic effects in both, plant and human cells, as well as antimicrobial properties. Further studies are needed to strengthen these findings.
Celotno besedilo
Dostopno za:
DOBA, IJS, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
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