Non-English title Sarmiento, Maria Mercedes; Mangiaterra, Magdalena; Bojanich, Maria Viviana ...
Revista iberoamericana de micología,
03/2016, Letnik:
33, Številka:
1
Journal Article
Recenzirano
Background: The soil is a natural reservoir of keratinophilic fungi, which are a small but important group of filamentous fungi, some of which typically develop on keratinized tissues of living ...animals. There are numerous species of saprophytic fungi with recognized keratinophilic abilities, and several studies have been undertaken in order to link their presence to possible human disease. Aims: To know the biota of geophilic fungi in general and of keratinophilic fungi particularly in soils from two public parks. Methods: Soil samples from two public parks of Corrientes city, Argentina, were studied during two seasons, using the hook technique and serial dilutions for fungal isolation. Results: Using the hook technique, 170 isolates were classified into 17 genera and 21 species, among which it is worth mentioning the presence of Microsporum canis. Shannon index for keratinophilic fungi in autumn was 2.27, and 1.92 in spring. By means of the serial dilutions technique, 278 fungi isolated were identified into 33 genera and 71 species. Shannon index in autumn was 3.9, and 3.5 in spring. Conclusions: The soils studied have particularly favorable conditions for the survival of pathogens and opportunistic geophilic fungi for humans and animals. Palabras clave * Hongos geofilos * Hongos queratinofilicos * Suelos
Dermatomycoses are common superficial cutaneous fungal infections which affect the skin, nails and human hairs. It affects 20 to 25% of the world population. The causative fungus varies ...geographically across the globe. Study on dermatomycoses is crucial to identify the aetiological fungus involved locally. The study aimed to determine the causative fungus of superficial fungal infections of the skin, nail and hair in patients presented to Hospital Melaka.
This was a prospective study conducted from 15th January 2022 till 15th October 2022 at Dermatology Clinic, Hospital Melaka. Subjects with clinical dermatomycoses were included in this study. The samples were collected from skin, nails and hairs clinically affected by tinea corporis/cruris/pedis, onychomycosis and tinea capitis respectively. A potassium hydroxide (KOH) study was performed on the sample in which the fungal hyphae/yeast positive subjects were sent for fungal culture and fungal PCR test.
A total of 222 clinical samples from skin, nails and hairs with a clinical suspicion of dermatomycoses yielded fungal hyphae/yeast in KOH. Majority of the samples were collected from skin (138, 62.2%), followed by nails (65, 29.3%) and hairs (19, 8.6%). Male to female ratio was 1.18: 1. The age ranged from 2 to 87 with the median of 55.5-yearsold. Out of 222 samples, 150 (67.6%) were fungal culture positive. From fungal culture positive samples, 87 samples were from tinea corporis, 50 samples were from onychomycoses and 13 samples were from tinea capitis. Trichophyton rubrum (39, 44.8%) was the commonest dermatophyte isolated in tinea corporis/cruris/pedis. Nondermatophyte moulds (NDM, 35, 70%) were the main fungi isolated in onychomycosis. Microsporum canis (7/53.8%) was the principal causative fungus among patients with tinea capitis. Among 150 fungal culture positive samples, 76 were fungal PCR positive. Only 38 samples consistently isolated same fungal species in both fungal culture and PCR test.
Majority of tinea corporis and tinea capitis fungal culture isolated dermatophytes, especially Trichophyton rubrum and Microsporum canis, respectively. Non-dermatophyte moulds were mainly isolated in onychomycosis.
The aim of this study was to investigate the clinical manifestations and mycological features of feline dermatophytosis in a population of cats with suspected lesions of dermatophytosis at Ferdowsi ...University of Mashhad Veterinary Teaching Hospital (northeast of Iran). Skin, hair, and nail samples from 122 clinically suspected cases of feline dermatophytosis were screened by direct microscopic examination. Of the 122 samples examined, 91 (74.59 %) were positive for fungal elements by direct microscopic examination, and 66 (54 %) were culture-positive for Microsporum canis. M. canis was the only dermatophyte species isolated from the cats. In the studied population, young cats (< 7 months) showed a statistically higher rate of M. canis infection than older animals (p < 0.001). The occurrence of dermatophytosis did not correlate with gender or the type of cats’ hair. The isolation rate of dermatophytes was relatively high in winter. However, the association between season and the dermatophyte inflation rate was not significant. The diagnostic value of microscopic examination proved to be high compared to fungal cultures as 64 of 66 samples that had positive M. canis culture were positive at microscopic examination. The agreement between culture and microscopic examination was moderate (k = 0.49). The information acquired in this study provides helpful insights into dermatophytoses in northern Iran highlighting the most important clinical and mycological features of skin mycoses in pet cats.
Background and Objective: Dermatophytosis is a significant skin disease in humans and animals, with increasing resistance to conventional treatments. This study aimed to determine the antifungal ...activity of petroleum ether, dichloromethane, ethyl acetate, ethanol, and hydroethanol extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against common dermatophytic fungi, including Trichophyton rubrum, Trichophyton verrucosum, Epidermophyton floccosum, and Microsporum canis. Methods: This descriptive study examined the antifungal activity of various extracts from the aerial parts of Artemisia khorassanica, Artemisia scoparia, and Artemisia vulgaris against fungal isolates of Trichophyton rubrum, Trichophyton verrucosum, Microsporum canis, and Epidermophyton floccosum. The aerial parts of the three Artemisia species were extracted using five solvents: petroleum ether, dichloromethane, ethyl acetate, ethanol, and 50% hydroethanol. The extracts were screened for antidermatophytic effects against the fungi mentioned above, and the minimum inhibitory concentration (MIC) was determined using the agar dilution method. The most active extracts underwent preliminary phytochemical testing. Results: In the initial screening, Microsporum canis and Trichophyton verrucosum showed the highest sensitivity (86.66%) and resistance (100%) to the tested extracts, respectively. Extracts from Artemisia scoparia exhibited the broadest range of activity. The most effective extracts, with an MIC of 78.12 µg/ml, were also from this plant. Hydroethanol extracts from all plant species demonstrated the least antidermatophytic activity. Preliminary phytochemical studies revealed the presence of terpenoids in all petroleum ether and dichloromethane extracts of the plant species. Conclusion: Some lipophilic compounds, particularly in the petroleum ether and dichloromethane extracts of Artemisia scoparia, exhibit significant in vitro antidermatophytic activity.
Multilocus microsatellite typing (MLMT) was performed on 93 strains of Microsporum canis isolated between 2012 and 2017 from 75 cats, eight dogs and 10 pet owners. These strains were derived from two ...major reservoirs: commercial breeding facilities and pet shops (PS), and stray cats and pet cats that went outdoors and came in contact with stray cats (Outdoor). Six microsatellite markers were used for genotyping. These 93 strains included 22 genotypes, 11 previously detected in Japan and 11 new genotypes. Strains belonging to the previously reported genotypes P and A were distributed widely throughout Japan. Genotype P was the most frequent, accounting for 37 (39.8%) of the 93 strains, most derived from Outdoor sources. Genotype A was the second most frequent, being present in 11 (11.8%) of the 93 strains, most derived from the PS reservoir. All new genotypes were detected in isolates from cats, with many of these derived from the Outdoor reservoir. The consistency of infection was shown in 18 of 19 familial cases. These findings indicate that genotypes differ in strains derived from PS and Outdoor reservoirs and that genotyping by MLMT is useful for tracking the routes of spread and transmission of M. canis in Japan.
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