In this review paper, different characteristics especially medicinal values of Oyster mushroom were described. As a mycological expression, mushrooms are a fruiting body of macro fungi i.e. ...Basidiomycota that represents only a short reproductive stage in their life cycle. They have a long association with humankind, and provide profound biological and economic impacts. Starting from ancient times, mushrooms are consumed by man with delicacy, due to their good taste and pleasing flavor. Pleurotus ostreatus is an edible mushroom with high nutritional values and biomedical importance's, since it contains a large number of bioactive components that cause development of its therapeutic functions. The bioactive components that are present in Pleurotus ostreatus mushroom comprise: polysaccharides, lipopolysaccharides, proteins, peptides, glycoproteins, nucleosides, triterpenoids, lectins, lipids and their derivatives, in addition of its vital medicinal components beneficial for the human health. Moreover, Pleurotus ostreatus possess several medicinal properties including; anti-arthritic, antitumor, immune-modulatory, antioxidant, anticancer, anti-inflammatory, antigenotoxic, hypo cholesterolaemic, anti-hyperglycaemic, anti-hypertensive, antiplatelet aggregating, antiviral and antimicrobial activities.
•Two modified polysaccharides (AcMZPS and AlMZPS) were isolated and purified.•Hyperlipidemic mice were established by high-fat-high-cholesterol emulsion.•The antioxidant and anti-hyperlipidemic ...effects were investigated.•Glucose was determined to the major monosaccharide component of polysaccharides.•Findings proved that AcMZPS and AlMZPS had prevention effects of hyperlipidemia.
The aims of this work were designed to investigate the hepatoprotective and antioxidant effects of acidic- and alkali-extractable mycelia zinc polysaccharides (AcMZPS, AlMZPS) from Pleurotus eryngii var. tuoliensis on high-fat-high-cholesterol emulsion-induced hyperlipidemic mice. The in vivo experiments demonstrated that both AcMZPS and AlMZPS had potential hepatoprotective effects by significantly decreasing the levels of LDL-C, VLDL-C, TC, TG, ALT, AST, ALP, MDA and LPO, and remarkably increasing the HDL-C, SOD, GSH-Px, and CAT in serum lipid/liver homogenate, respectively. In addition, four polysaccharide fractions of AcMZPS-1, AcMZPS-2, AlMZPS-1, and AlMZPS-2, purified from AcMZPS and AlMZPS using DEAE chromatography, respectively, were subjected to monosaccharide composition analysis and valuated for the in vitro antioxidant activity. The results obtained in present study suggested that AcMZPS, AlMZPS and their purified fractions could be used as functional foods and natural drugs in preventing the hyperlipidemia and non-alcoholic fatty liver.
Hyperbranched polysaccharide from Pleurotus tuber-regium (PTR-HBPS) is a β-glucan with high degree of branching (DB, 0.69) and a molecular weight (Mw) of 31.2 × 105 g/mol with mixed β-1, 4/β-1, 4, ...6/β-1, 6 glucosidic linkages. PTR-HBPS was depolymerized by cellulase and β-glucosidase under optimized conditions to form PC (PTR-HBPS depolymerized by cellulase) and PG (PTR-HBPS depolymerized by β-glucosidase) fractions with a minimum Mw of 2.74 × 105 and 3.98 × 105 g/mol, respectively. PC fractions had no significant changes for its primary structure in terms of glycosidic linkages, DB, and triple helical structure, while the DB of PG fractions was reduced to 0.63 with the loss of triple helical structure. Nanoparticles fabricated by PC fractions with zein showed better stability under different pH conditions. Enzymatic depolymerized low Mw β-glucan derived from PTR-HBPS with similar structural characteristics as the native one has potential as nanocarriers for food bioactive substances.
•Cellulase could depolymerize Mw of PTR-HBPS up to one-eleventh of its original size.•Cellulase cleaved β-1,4 glucosidic bonds in backbone but did not change configuration.•β-Glucosidase could degrade Mw of PTR-HBPS up to one-eighth of its original size.•β-Glucosidase cuts more side branches from non-reducing end caused a decrease of DB.•PC3/zein nanoparticles have better pH stability.
To study the effect of polythene colour container and spawn rates on mushroom (Pleurotus ostreatus), experiment was carried out to investigate the cultivation on barely straws substrate. This ...cultivation system can improve the economic status of the farmer. Field experiment was conducted at College of Agriculture, University of Zabol during 2011 growing season. Experiment was carried out as factorial and based on completely randomized design (CRD) with three replications. Factors were different colour of polythene container (blue, yellow, transparent and green) and various levels of spawn (2.5, 4 and 5% wet weight substrate bases). There was significant difference in the yields from different spawn and colour of container maximum yield (weight of fresh mushrooms harvested at maturity) was obtained in 5% spawn rate (1248 g/2 kg wet substrate). Between colour polythene containers; maximum yield was obtained in green colour polythene (1366 g/2 kg wet substrate). The least of yield mushrooms were obtained from 2.5% spawn rate and cultivation of mushroom in blue polythene container. It was concluded that the mushroom should be cultivated in green polythene bags under 5% spawn rate to achieve higher productivity.
The function of small secreted proteins (SSPs) in saprotrophic fungi is, for the most part, unknown. The white-rot mushroom
produces considerable amounts of SSPs at the onset of secondary metabolism, ...during colony development, and in response to chemical compounds such as 5-hydroxymethylfurfural and aryl alcohols. Genetic manipulation of Ssp1, by knockdown (KD
) or overexpression (OE
), indicated that they are, in fact, involved in the regulation of the ligninolytic system. To elucidate their potential involvement in fungal development, quantitative secretome analysis was performed during the trophophase and the idiophase and at a transition point between the two growth phases. The mutations conferred a time shift in the secretion and expression patterns: OE
preceded the entrance to idiophase and secondary metabolism, while KD
was delayed. This was also correlated with expression patterns of selected genes. The KD
colony aged at a slower pace, accompanied by a slower decline in biomass over time. In contrast, the OE
strain exhibited severe lysis and aging of the colony at the same time point. These phenomena were accompanied by variations in yellow pigment production, characteristic of entrance of the wild type into idiophase. The pigment was produced earlier and in a larger amount in the OE
strain and was absent from the KD
strain. Furthermore, the dikaryon harboring OE
exhibited a delay in the initiation of fruiting body formation as well as earlier aging. We propose that Ssp1 might function as a part of the fungal communication network and regulate the pattern of fungal development and metabolism in
Small secreted proteins (SSPs) are common in fungal saprotrophs, but their roles remain elusive. As such, they comprise part of a gene pool which may be involved in governing fungal lifestyles not limited to symbiosis and pathogenicity, in which they are commonly referred to as "effectors." We propose that Ssp1 in the white-rot fungus
regulates the transition from primary to secondary metabolism, development, aging, and fruiting body initiation. Our observations uncover a novel regulatory role of effector-like SSPs in a saprotroph, suggesting that they may act in fungal communication as well as in response to environmental cues. The presence of Ssp1 homologues in other fungal species supports a common potential role in environmental sensing and fungal development.
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•Crop side-streams were substrates for solid-state fermentation with P. sapidus strains.•A monokaryon strain was used as an innovative source of mycelia to valorize the biomass.•The ...phenolic content was reduced after fermentation with monokaryon and dikaryon strains.•The extracts of fermented biomass had lower antioxidant and cytotoxic activities.•Fermentation with P. sapidus can impact on the phenolic profile of the raw material.
Fungi are known to modify the properties of lignocellulosic materials during solid-state fermentation (SSF). In this study, agricultural side-streams (sunflower seed hulls, rice husks and rice straw) were used as substrates for SSF with dikaryotic and monokaryotic strains of Pleurotus sapidus. The phenolic profiles of the mentioned substrates were characterized by LC-DAD/ESI-MSn pre- and post- fermentation. Moreover, antioxidant, cytotoxic and antimicrobial activities were screened against oxidizable cellular substrates, tumour and primary cell lines, and different bacteria and fungi, respectively. The concentration of phenolic compounds in the crop side-streams was reduced after fermentation with both strains of the fungus. The fermented extracts also displayed lower antioxidant and cytotoxic activities and had no hepatotoxicity. The antimicrobial activity depended upon the crop side-stream and/or SSF conditions. These results indicate that P. sapidus represent a good candidate to modify the phenolic fraction presents in crop side-streams with a consequent decrease in its bioactivities. However, the SSF with P. sapidus strains play an interesting role in the detoxification of plant materials which can be used for different applications according to the “reduce - reuse - recycle” concept contributing with the sustainable land use and circular economy.
This study assessed the bioconversion of Agriculture wastes like invasive weeds species (Lantana camara, Prosopis juliflora, Parthenium hysterophorus) as a substrate for oyster mushroom (Pleurotus ...species) cultivation together with wheat straw as a control. The experiment was laid out in factorial combination of substrates and three edible oyster mushroom species in a Completely Randomized Design (CRD) with three replications. Pleurotus ostreatus gave significantly (p < 0.01) total yield of 840 g kg(-1) on P. hysterophorus, Significantly (p < 0.01) biological efficiency (83.87%) and production rate of 3.13 was recorded for P. ostreatus grown on P. hysterophorus. The highest total ash content (13.90%) was recorded for P. florida grown on L. camara. while the lowest (6.92%) was for P. sajor-caju grown on the P. juliflora. Crude protein ranged from 40.51-41.48% for P. florida grown on P. hysterophorus and L. camara. Lowest crude protein content (30.11%) was recorded for P. ostreatus grown on wheat straw. The crude fiber content (12.73%) of P. sajor-caju grown on wheat straw was the highest. The lowest crude fiber (5.19%) was recorded for P. ostreatus on P. juliflora. Total yield had a positive and significant correlation with biological efficiency and production. Utilization of the plant biomass for mushroom cultivation could contribute to alleviating ecological impact of invasive weed species while offering practical option to mitigating hunger and malnutrition in areas where the invasive weeds became dominant.
A novel positive single-stranded RNA virus, Pleurotus ostreatus deltaflexivirus 1 (PoDFV1), was isolated from the edible fungus
Pleurotus ostreatus
strain ZP6. The complete genome of PoDFV1 is 7706 ...nucleotides (nt) long and contains a short poly(A) tail. PoDFV1 was predicted to contain one large open reading frame (ORF1) and three small downstream ORFs (ORFs 2-4). ORF1 encodes a putative replication-associated polyprotein of 1979 amino acids (aa) containing three conserved domains – viral RNA methyltransferase (Mtr), viral RNA helicase (Hel), and RNA-dependent RNA polymerase (RdRp) – which are common to all deltaflexiviruses. ORFs 2-4 encode three small hypothetical proteins (15-20 kDa) without conserved domains or known biological functions. Sequence alignments and phylogenetic analysis suggested that PoDFV1 is a member of a new species in the genus
Deltaflexivirus
(family
Deltaflexiviridae
, order
Tymovirales
). To our knowledge, this is the first report of a deltaflexivirus infecting
P. ostreatus
.
Cultivation of speciality mushrooms on lignocellulosic wastes represents one of the most economically and cost-effective organic recycling processes. Three species of Pleurotus, namely P. columbinus, ...P. sajor-caju and P. ostreatus were experimentally evaluated on untreated organic wastes including chopped office papers, cardboard, sawdust and plant fibres. Production studies were carried out in polyethylene bags of about 1kg wet weight with 5% spawning rates of substrate fresh weight in a custom-made growth room especially designed for spawn run and cropping. The conversion percentage from dry substrate weight to fresh mushroom weight (biological efficiency) was determined. The highest biological efficiency was noted with P. columbinuson cardboard (134.5%) and paper (100.8%), whereas P. ostreatus produced maximum yield on cardboard (117.5%) followed by paper (112.4%). The overall yield of P. sajor-cajuwas comparatively low (range 47-78.4%). The average number of sporophore flushings ranged between 5 and 6 times. The findings that P. columbinus and P. ostreatus are superior to P. sajor-caju are consistent with previous reports elsewhere. Further evaluation of P. columbinus alone on different bagging systems containing partially pasteurized office papers as a growing substrate revealed that polyethylene bags resulted in 109.4% biological efficiency in contrast to pottery (86%), plastic trays (72%) or polyester net (56%). The above findings reveal an opportunity for commercial implication of oyster mushroom especially P. columbinus for utilization of different feasible and cheap recyclable residues.PUBLICATION ABSTRACT
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•RS was pretreated with three different fungal strains (PC, PO and GL).•Lignocellulosic degradation showed the change in structural and morphological features after ...pretreatment.•Enhanced methane yield was observed (1.64–2.22-fold).•MGM explained the results more effectively in comparison to LGM and TFM.
The influence of three different fungal strains—namely, Pleurotus ostreatus (PO), Phanerochaete chrysosposrium (PC), and Ganoderma lucidum (GL)—on pretreatment of rice straw, followed by biochemical methane potential assay was evaluated on the basis of structural (Field Emission Scanning Electron Microscopy, X-ray diffraction etc.) and quantitative (soluble chemical oxygen demand, volatile fatty acids, etc.) analysis. Maximum lignocellulosic degradation was obtained with PC pretreated rice straw (36% more than an untreated sample), followed by PO. Enhancement in the methane yield after 5 weeks of inoculation time was obtained after pretreatment, which was 269.99, 295.91, and 339.31 mL/g VSadded, for PO, GL, and PC, respectively, 1.64–2.22-fold higher than the untreated one. Kinetic modelling of cumulative methane yield showed that modified gompertz model showed the best fit among all analysed models. This study demonstrated the usefulness of fungal species in enhancing the methane yield.
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