► Alginates can be crosslinked with various cations to modify the characteristics of the resultant matrices for different applications. ► New strategies have been successfully employed to improve the ...gelation mechanism of alginates. ► Innovative wound dressings with haemostatic, absorbent and antimicrobial properties have been developed. ► A number of patents involving alginate formulations for cell transplant have emerged. ► Alginates can be employed for rapid, as well as prolonged delivery of bioactive compounds.
This review outlines the role of alginates in microencapsulation and therapeutic applications. It focuses on the physicochemical properties of alginates (e.g. viscosity, thermo-stability, sol–gel transformation and drug release) to gain better insight into their potential medical applications, particularly for wound care and therapeutics. In order to understand how alginates can be optimized as a useful delivery system for therapeutic applications, various factors that impact drug release from alginate matrices (e.g. types of cations used in cross-linking, porosity of alginate matrices, pH effect, alginate composition, molecular weight of encapsulated drugs and modification of the functional groups in alginates) are also discussed. More specifically, practical applications of the cross-linking mechanism and sol–gel transformation property of alginates are explored to assess their potential to improve the mechanical properties of alginate dressings, to impart anti-microbial properties for treating wound infections and to develop products for tissue repair and wound healing. Innovative processes of developing alginate carriers and delivery systems and their recent applications are also discussed. Strategies employed to improve gelation of alginates commonly target the formulation by the inclusion of non-gelling cations or sequestrants during cross-linking. The application of other strategies, such as hot-made alginate gel method,
in situ gelation method, crystal gun method, acoustic excitation method, and the use of extrusion devices with improved design are reviewed.
Oligosakarida alginat (OSA) adalah produk hasil depolimerisasi polimer alginat yang biasanya terdiri dari 2-25 monomer. Produksi OSA dapat dilakukan melalui proses enzimatis, fisik maupun kimiawi. ...Tujuan penelitian ini adalah mengetahui karakteristik dan bioaktivitas OSA sebagai antioksidan dan inhibitor α-glukosidase. Proses produksi OSA dilakukan secara enzimatis dengan 3 cara penambahan alginat liase yaitu ditambahkan satu kali di awal reaksi (E1), ditambahkan 4 kali dengan interval 2 jam (E2) dan 2 kali interval 4 jam (E3) dengan total volume sama, yaitu 0,15 mL dan aktivitas enzim (1 unit/mL). Total waktu inkubasi adalah 8 jam. Karakterisasi OSA yang dilakukan adalah perhitungan rendemen, analisis profil TLC dan FTIR serta kadar gula pereduksi. Metode DPPH (1,1-diphenyl-2-picrylhydrazyl) digunakan untuk menguji aktivitas antioksidan OSA, sedangkan pengamatan terhadap aktivitas inhibitor α-glukosidase dilakukan dengan melihat aktivitas α-glukosidase dalam mengubah substrat yang diberikan. Hasil penelitian menunjukkan bahwa rendemen OSA dan kadar gula pereduksi tidak berbeda nyata antar perlakuan dengan rendemen OSA berkisar antara 77,29±1,97% hingga 85,46±9,15% dan kadar gula pereduksi OSA berkisar antara 290,32±20,42 µg/mL hingga 312,76±4,74 µg/mL. Aktivitas antioksidan tertinggi diperlihatkan oleh OSA E1 dengan penghambatan terhadap DPPH sebagai radikal bebas sebesar 41,22±2,03% pada konsentrasi 1,2 mg/mL. Aktivitas inhibitor α-glukosidase OSA E1 lebih kecil dibandingkan dengan alginat dengan nilai IC50 masing-masing sebesar 11,23±4,17 ppm dan 5,27±0,29 ppm. Proses depolimerisasi alginat meningkatkan aktivitas alginat sebagai antioksidan namun tidak meningkatkan aktivitasnya sebagai inhibitor α-glukosidase. AbstractAlginate oligosaccharides (AOS) is depolymerization of alginate polymer product that consist of 2-25 monomers. Alginate oligosaccharides can be produced by enzymatic, physical and chemical processes. This research was conducted to find out the characteristics and bioactivity of AOS as an antioxidant and α-glucosidase inhibitor. AOS was produced by enzymatic process with 3 procedures of the addition of the alginate lyase that was added once at the beginning reaction (E1), 4 times every 2 hours (E2) and 2 times every 4 hours (E3) with the same addition of enzyme volume (0.15 mL) and enzyme activity (1 unit/mL). Total incubation times was 8 hours. Alginate oligosaccharides was then characterized their yield, TLC, FTIR profiles and reducing sugar content. DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to determine antioxidant activity of AOS while observation of α-glucosidase activity in changing the substrate was used to determine inhibitor α-glucosidase activity of AOS. The results showed that yields and reducing sugar level of AOS were not significantly different between treatments. The AOS yields ranged from 77.29±1.97% to 85.46±9.15% and the reducing sugar levels ranged from 290.32±20.42 µg/mL to 312.76±4.74 µg/mL. The highest antioxidant activity was shown by AOS E1 with free radical (DPPH) inhibition of 41.22±2.03%. AOS E1 α-glucosidase inhibitor activity was lower than that of alginate, with the IC50 values of 5.27±0.29 ppm for alginate and 11.23±4.17 ppm for AOS. Depolymerization process of alginates increased antioxidant activity but did not enhance its activity as α-glucosidase inhibitor.
Bioplastics are plastics made from renewable raw materials such as polysaccharides, proteins and lipids. One of the alternative sources of bioplastic raw materials is hydrocolloid from seaweed, which ...is abundantly available in Indonesia, so that this hydrocolloid-based bioplastic is very prospective to be developed, and can increase the added value of seaweed. The physical and mechanical properties of alginate bioplastics can be improved by combining them with other materials into biocomposite materials that have superior properties and meet specifications. This study aims to determine the effect of calcium chloride (CaCl2) on the physical and mechanical properties of the CMC-Glycerol-Alginate composite bioplastic from Sargassum sp. Bioplastics were made by mixing 0.5 g of alginate flour, added CMC (1.5 g), and 100 ml of distilled water, then stirred with a magnetic stirrer for 10 minutes at 90oC. After that, the temperature was lowered to 40oC and 5 ml of glycerol was added and then homogenized again for 15 minutes. The mixture was filtered and then poured into a glass mold and the surface was leveled using a stainless steel cylinder, then dried in an oven at 80oC for 12 hours. After that the bioplastic is released from the glass plate. In the soaking method, the bioplastic sheets were immersed in a 2% CaCl¬2 solution for 5 minutes, then dried and stored in a desiccator. In the mixing method, 1 gram of CaCl¬2 was put directly into the alginate-CMC-glycerol mixture and homogenized with a magnetic stirrer at 90oC for 15 minutes, then printed on a glass plate, then dried at 100oC for 12 hours. CaCl2 treatment by mixing and soaking decreased elongation, tensile strength, biodegradability and transparency, but increased water resistance and thickness of the alginate-CMC-glycerol composite bioplastic, and changed the surface properties of the bioplastic to be rougher. No new functional groups were formed due to the interaction between alginate, CMC, glycerol, distilled water and CaCl2. Bioplastik adalah plastik yang dibuat dari bahan baku terbarukan seperti polisakarida, protein dan lipida. Salah satu alternatif sumber bahan baku bioplastik adalah hidrokoloid dari rumput laut yang tersedia melimpah di Indonesia, sehingga bioplastik berbahan hidrokoloid ini sangat prospektif untuk dikembangkan, serta dapat meningkatkan nilai tambah rumput laut.Sifat fisik dan mekanik bioplastik alginat dapat ditingkatkan dengan cara dikombinasi dengan bahan lain menjadi material biokomposit yang memiliki sifat unggul dan memenuhi spesifikasi.Penelitian ini bertujuan untuk mengetahui pengaruh kalsium klorida (CaCl2) terhadap sifat fisik dan mekanik bioplastik komposit CMC- Gliserol-Alginat dari Sargassum sp.Bioplastik dibuat mdengan mencampurkan tepung alginat sebanyak 0,5 g ditambahkan CMC ( 1,5 g), dan akuades 100 ml, lalu diaduk dengan magnetic stirrer selama 10 menit pada suhu 90oC. Setelah itu, suhu diturunkan sampai 40oC dan ditambahkan gliserol 5 ml lalu dihomogenkan lagi selama 15 menit. Campuran disaring lalu dituang dalam cetakan kaca dan diratakan permukaannya menggunakan silinder stainless steel, kemudian dikeringkan dalam oven pada suhu 80oC selama 12 jam. Setelah itu bioplastik dilepaskan dari pelat kaca. Pada metoda soaking lembaran bioplastik direndam dalam larutan CaCl2 2% selama 5 menit, lalu dikeringkan dan disimpan dalam desikator. Pada metoda mixing, CaCl2 sebanyak 1 gram dimasukkan langsung ke dalam campuran alginat-CMC-gliserol dan dihomogenkan dengan magnetic stirrer pada suhu 90oC selama 15 menit, lalu dicetak dalam pelat kaca, lalu dikeringkan pada suhu 100oC selama 12 jam. Perlakuan CaCl2 dengan cara mixing dan soaking menurunkan elongasi, kuat tarik, biodegradabilitas dan transparansi, tetapi meningkatkan ketahanan air dan ketebalan bioplastik komposit alginat-CMC-gliserol, serta mengubah sifat permukaan bioplastik menjadi lebih kasar. Tidak terdapat gugus fungsi baru yang terbentuk akibat interaksi antara alginat, CMC, gliserol, akuades dan CaCl2.
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•Honey/SA/PVA nanofibers were fabricated via all-aqueous electrospinning.•The effect of honey content on the swelling ratio of membrane was investigated.•Honey enhanced the ...antioxidant and antibacterial activities of nanofibers.•Honey/SA/PVA nanofiber membrane exhibited good biocompatibility.
Honey is an ancient natural wound-healing agent and has been reintroduced to modern clinical wound care as it has various bioactivities. In this study, honey was incorporated into an alginate/PVA-based electrospun nanofibrous membrane to develop an efficient wound dressing material. The morphology and chemical composition of the nanofibrous membrane were observed by scanning electron microscopy and characterized via Fourier transform infrared spectroscopy, respectively, demonstrating that honey was successfully introduced to the nanofibers. The nanofibrous membranes with increasing honey content showed enhanced antioxidant activity, suggesting the ability to control the overproduction of reactive oxygen species. Disc diffusion assay and dynamic contact assay proved the antibacterial activity of the honey loaded nanofibers towards Gram-positive bacterium (Staphylococcus aureus) and Gram-negative bacterium (Escherichia coli). The cytotoxicity assay illustrated the non-cytotoxicity and biocompatibility of the nanofibrous membranes. Therefore, the developed honey/alginate/PVA nanofibrous membranes are promising for wound dressings.
Microstructured calcium alginate (Ca-Alg) hydrogel exhibiting superhydrophilicity and underwater superoleophobicity is prepared for high speed and highly efficient oil/water separation. The ...fabricated mesh works in highly acidic or basic, salty, and high-temperature environments because of the stability of Ca-Alg. Moreover, nonwoven fabric used as a template for Ca-Alg is capable of separation of an oil-in-water emulsion.
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•Dual-physically cross-linked double network endows hydrogel recoverability and fatigue resistance.•Ca2+ ions provide hydrogel excellent strain-sensitive conductivity.•The hydrogel ...can be assembled as wearable strain sensor to monitor human-motions.•The monitor of slight physiological activities like talking and breathing were achieved.
Soft, stretchable, and elastic hydrogels have recently attracted immense interest due to their potential applications in wearable strain sensors. However, most of hydrogel-based sensors exhibit poor mechanical properties. Here, a robust, flexible and strain-sensitive conductive wearable sensor is prepared based on dual physically cross-linked double network hydrogels consisting of core-shell hybrid nanoparticles cross-linked polyacrylamide as first network and Ca2+ cross-linked alginate as second network. Dynamic physical cross-linking as sacrifice bonds can effectively dissipate energy and reconstruct the network structure, endowing hydrogels with high strength, toughness, stretchability and excellent self-recovery properties. Moreover, the hydrogels exhibit outstanding strain sensitive behavior with repeatable, stable, and precise changes in resistance signals. Based on the excellent strain sensitivity, the hydrogel can be assembled as a wearable strain sensor to monitor joint motions such as finger, wrist, elbow, neck, and knee joints, and even the slight motions including breathing and speaking. Thus, the tough, anti-fatigue, self-recovery and conductive hydrogels have promising potential as soft, high-performance and flexible wearable strain sensor for soft robots, biomimetic prostheses, human activity monitoring and health-monitoring systems.
Tough hydrogels, polymeric network structures with excellent mechanical properties (such as high stretchability and toughness), are emerging soft materials. Despite their remarkably mechanical ...features, tough hydrogels exhibit two flaws (freezing around the icing temperatures of water and drying under arid conditions). Inspired by cryoprotectants (CPAs) used in the inhibition of the icing of water in biological samples, a versatile and straightforward method is reported to fabricate extreme anti‐freezing, non‐drying CPA‐based organohydrogels with long‐term stability by partially displacing water molecules within the pre‐fabricated hydrogels. CPA‐based Ca‐alginate/polyacrylamide (PAAm) tough hydrogels were successfully fabricated with glycerol, glycol, and sorbitol. The CPA‐based organohydrogels remain unfrozen and mechanically flexible even up to −70 °C and are stable under ambient conditions or even vacuum.
Anti‐freezing, non‐drying tough organohydrogels are fabricated by a solvent displacement strategy. Water molecules in the hydrogels are partially displaced by cryoprotectants, as inspired by the cryopreservation of biological samples. This method opens a way to rationally tune the freezing and drying tolerance for many water‐based hydrogels.
This work represents a contribution to the design, preparation, and characterization of nanocomposite materials based on biocompatible components. The effects of composition, filler geometry, and ...polymer charge were highlighted, and their role on the final properties of the nanocomposites was revealed. We combined some biopolymers (methylcellulose, alginate, chitosan) with two nanoclays (kaolinite sheets and halloysite nanotubes) to prepare nanocomposites by means of the casting method from water. The thermal stability, the surface wettability, and the mechanical properties of the obtained films were studied. SEM micrographs highlighted the surface morphology of the biocomposite materials. X-ray data allowed us to correlate the mesoscopic structure to the properties of these nanocomposites.
A smart valve is created by 4D printing of hydrogels that are both mechanically robust and thermally actuating. The printed hydrogels are made up of an interpenetrating network of alginate and ...poly(N‐isopropylacrylamide). 4D structures are created by printing the “dynamic” hydrogel ink alongside other static materials.
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