Introduction
Spontaneously hyperlipidemic (SHL) mice, a mouse strain derived from an inbred strain of Japanese wild (original)-type mice (KOR; Mus musculus molossinus), show high plasma cholesterol ...concentrations with disruption of the apolipoprotein E (Apoe) gene. However, the details of the Apoe gene of SHL mice have yet to be described.
Material and methods
The DNA sequence of the Apoe gene of SHL mice was compared to that of control KOR mice in genomic DNA and cDNA analyses.
Results
In the DNA analysis, a 4700-bp fragment was found to be inserted into exon 4 of the Apoe gene of SHL mice. The insertion contained two 365-bp repeats at each terminal and was flanked by a 6-bp target duplication at each side. The inserted fragment produced a frameshift of an early stop codon, resulting in a protein product that consisted of 87 amino acids in SHL mice compared to 311 amino acids in control KOR mice.
Conclusions
These findings provide useful information about the molecular basis of SHL mice and related lipid disorders.
Unsettled science on longer-run effects of early education Burchinal, Margaret; Whitaker, Anamarie; Jenkins, Jade ...
Science (American Association for the Advancement of Science),
05/2024, Letnik:
384, Številka:
6695
Journal Article
Recenzirano
More rigorous research is needed on how to design programs.
