Osnovni razlogi za zbiranje domačih vzorcev ajde po Sloveniji so bili predvsem v opuščanju pridelovanja domačih populacij in nadomestitev s tujimi uvoženimi sortami in populacijami, katerih seme je ...bilo na razpolago v zadostni količini pridelovalcem. Drug razlog je bil v nevarnosti, da se domače populacije skrižajo s tujim uvoženim materialom ali z novimi slovenskimi sortami in kot tretji razlog zbran material je nudil veliko variabilnost in možnost izbire ter uporabe kot vir genov za žlahtniteljsko delo. Pogosto so tam, kjer so se oz. se z žlahtnjenjem še ukvarjajo, kot potreba nastajale zbirke posameznih rastlinskih vrst. To so bili glavni razlogi pred 30 in več leti za zbiranje in reševanje gen fonda. Glede na dosedanje opise, bi lahko v grobem razdelili zbrani material v 2 skupini. Vzorce s sivimi semeni, ki imajo drobna siva semena in bele cvetove, le pri nekaterih populacijah se pojavljajo posamične rastline z rahlo roza cvetovi. Prilagojene so nižinskim in gričevnatim talnim in podnebnim razmeram, legam brez pogostih zgodnjih jesenskih slan in megla. V drugi skupini so populacije z nekoliko debelejšimi, temnimi - rjavimi semeni, osnovna barva cvetov je svetlo do temno roza, lahko se pojavijo posamične rastline z rahlo rdečimi cvetovi. Primerne so za višinske, hribovite lege s 7 do 10 dni krajšo rastno dobo. Konec septembra so primerne za žetev, saj jim v ugodnih klimatskih razmerah do takrat že odpade listje. Pri sivih populacijah pa listje odpade šele po prvih jesenskih slanah. Vzorci iz genske banke v preteklosti niso služili samo kot izhodiščni material za žlahtniteljsko delo (slovenske sorte ajde), ampak se je proučevala vitalnost semen, vsebnost polifenolov (tanina) v posameznih delih semen, genetska variabilnost in identifikacija vzorcev na nivoju DNA ter regeneracijska sposobnost v in vitro razmerah. Značilnost te zbirke je, da se je več ne dopolnjuje z domačimi populacijami, ker jih pridelovalci ne pridelujejo in jih na terenu ni možno več nabrati oz. dobiti.
The primary reason for collecting domestic samples of buckwheat in Slovenia is the abandonment of growing domestic populations and their replacement with foreign, imported species and populations, whose seed is available in sufficient quantity to growers. Another reason is the threat that domestic populations will cross with foreign imported material or with new Slovenian varieties. A third reason is that the collected material provides a lot of variability and the possibility of choice and use as a source of genes for breeding work. Breeding has often been accompanied with the collection of selected plant species that were required and used during the breeding process. More than 30 years ago, therefore, a buckwheat gene fund was created. Based on existing descriptions, the collected material can be roughly divided into two groups. Samples with small gray seeds mainly have white flowers, although individual plants with slightly pink flowers emerge in some populations. They are adapted to lowland and hilly soil and climatic conditions, as well positions without frequent or early autumn hoarfrosts and mist. The second group includes populations with slightly thicker dark - brown seeds; the basic color of the flowers is light to dark pink, although individual plants with slightly red flowers may emerge. They are suitable for higher altitude, mountainous locations with a 7 to 10 days shorter growth period. They are suitable for harvest at the end of September because, with good weather conditions, the leaves have already fallen off by then. In the case of grey populations, the leaves do not fall until the first autumn hoarfrosts. Samples from the gene bank have not only served as starting material for breeding work in the past (Slovenian buckwheat varieties) but also for investigation of the seed viability, of the seed content of polyphenols (tannins) in individual parts of seeds, genetic variability, identification of samples on the level of DNA and regeneration capacity under in vitro conditions. A characteristic of this collection is that it is no longer being supplemented by local populations because these are no longer to be found in the natural environment.
Z razvojem molekularne genetike so postale inbridirane linije učinkovito in nepogrešljivo orodje v biomedicinskih raziskavah. Njihova uporabnost sega od raziskovanja osnovnih mehanizmov delovanja ...genov in genskega uravnavanja do razvoja modelov za človeške bolezni, ki so nepogrešljivi pri raziskovanju patofizioloških, biokemičnih in molekularnih mehanizmov nastanka in razvoja bolezni kot tudi pri razvijanju novih terapevtskih sredstev. Zato je povsem razumljivo, da so zahteve po uniformnih in avtentičnih živalih znotraj genetsko standardiziranih linij upravičene in s stališča verodostojnosti rezultatov nujne. Namen prispevka je opozoriti na potencialne vire genetskega razhajanja med linijami in predstaviti metode, ki se lahko uporabljajo pri genetskem nadzoru glodalcev (predvsem laboratorijskih miši in podgan) tako v vzrejnih kot poskusnih enotah.
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