Background
High‐altitude pulmonary oedema (HAPE) is a form of noncardiogenic pulmonary oedema. Studies have found that long noncoding RNA (lncRNA) plays an important role in HAPE. ANRIL is ...significant in pulmonary illnesses, which implies that alterations in ANRIL expression levels may be involved in the beginning and development of HAPE. However, the specific mechanism is indistinct. The present study is meant to explore the effect and mechanism of ANRIL on hypoxic‐induced injury of pulmonary microvascular endothelial cells (PMEVCs).
Methods
In the hypoxic model of PMVECs, overexpression of ANRIL or knockdown of miR‐181c‐5p was performed to assess cell proliferation, apoptosis, and migration. Furthermore, the levels of apoptosis‐related proteins, inflammatory factors, and vascular active factors were also measured.
Results
The results showed that, after 24 h of hypoxia, PMVECs proliferation and migration were suppressed in comparison to the control group, along with an increase in apoptosis, a decrease in the expression of ANRIL, and an increase in the expression of miR‐181c‐5p (all p < .05). The damage caused by hypoxia in PMVECs can be lessened by overexpressing ANRIL, which also inhibits the production of TNF‐α, iNOS, and VEGF as well as BAX and cleaved caspase‐3 (all p < .05). Further experimental results showed that overexpression of ANRIL and knockdown of miR‐181c‐5p had the same protection against hypoxic injury in PMVECs (all p < .05).
Conclusions
Our study suggests that ANRIL may prevent hypoxia injury to PMVECs in HAPE through the negative regulation of miR‐181c‐5p.
Our study demonstrated that the proliferation and migration of PMVECs were suppressed after induction of hypoxia compared to the control group. Additionally, there was an increase in apoptosis, a decrease in ANRIL expression, and an increase in miR‐181c‐5p expression observed. Overexpressing ANRIL can alleviate hypoxia‐induced damage in PMVECs, as well as inhibit the production of TNF‐α, iNOS, VEGF, BAX, and cleaved caspase‐3. Furthermore, both overexpressing ANRIL and knocking down miR‐181c‐5p offered similar protection against hypoxic injury in PMVECs.
The ongoing Covid-19 is a contagious disease, and it is characterised by different symptoms such as fever, cough, and shortness of breath. Rising concerns about Covid-19 have severely affected the ...healthcare system in all countries as the Covid-19 outbreak has developed at a rapid rate all around the globe. Intriguing, a clinically used drug, acetazolamide (a specific inhibitor of carbonic anhydrase, CA, EC 4.2.1.1), is used to treat high-altitude pulmonary oedema (HAPE), showing a high degree of clinical similarities with the pulmonary disease caused by Covid-19. In this context, this preliminary study aims to provide insights into some factors affecting the Covid-19 patients, such as hypoxaemia, hypoxia as well as the blood CA activity. We hypothesise that patients with Covid-19 problems could show a dysregulated acid–base status influenced by CA activity. These preliminary results suggest that the use of CA inhibitors as a pharmacological treatment for Covid-19 may be beneficial.
Celotno besedilo
Dostopno za:
DOBA, IZUM, KILJ, NUK, PILJ, PNG, SAZU, UILJ, UKNU, UL, UM, UPUK
High altitude pulmonary oedema (HAPO) is a common emergency seen at high altitude. It can be associated with electrocardiogram (ECG) changes due to pulmonary arterial hypertension in the form of ST ...elevation and T wave inversion in the right precordial leads, which mimic acute coronary syndrome. These changes can lead to confusion in diagnosis and management. ECG changes resolve over a period of time when the patients are de-inducted to low land. So appropriate history and clinical examination, followed by monitoring of patients with cardiac enzymes and ECG, can prevent misdiagnosis and thereafter management.
•Interstitial lung water accumulation at altitude might precede high-altitude pulmonary oedema development.•We studied independent effects of hypobaria on interstitial lung water accumulation ...following hypoxic exercise in prematurely born adults.•Short, moderate-intensity exercise provokes a significant increase in the interstitial lung water accumulation after 8 h of exposure to terrestrial but not simulated altitude.
We aimed to gauge the interstitial lung water accumulation following moderate-intensity exercise under normobaric and hypobaric hypoxic conditions in a group of preterm born but otherwise healthy young adults.
Sixteen pre-term-born individuals (age = 21±2yrs.; gestational age = 29±3wk.; birth weight = 1160±273 g) underwent two 8 -h hypoxic/altitude exposures in a cross-over manner: 1) Normobaric hypoxic exposure (NH; FIO2 = 0.142±0.001; PIO2 = 90.6±0.9 mmHg) 2) Hypobaric hypoxic exposure (HH; terrestrial high-altitude 3840 m; PIO2 = 90.2±0.5 mmHg). Interstitial lung water was assessed via quantification of B-Lines (using lung ultrasound) before (normoxia) and after 4-h and 8-h of respective exposures. At each time point, B-Lines were quantified before (Pre) and immediately after (Post) a 6-min moderate-intensity exercise.
The baseline B-lines count were comparable between both conditions (P = 0.191). A higher B-lines count was noted at Pre-H4 in HH versus NH (P = 0.0420). At Post-H8 B-lines score was significantly higher in HH (4.6 ± 1.6) than in NH (3.1 ± 1.4; P = 0.0073). Furthermore, at this time point, a significantly higher number of individuals with B-line scores ≥5 was observed in HH (n = 7) than in NH (n = 3; P = 0.0420).
These findings suggest that short moderate-intensity exercise provokes a significant increase in the interstitial lung water accumulation after 8 h of exposure to terrestrial but not simulated altitude (≈3840 m) in prematurely born adults. Further work is needed to elucidate the exact mechanisms of (moderate-intensity) exercise-induced interstitial lung water accumulation in this population and directly compare the obtained data to full-term born adults.
Background and Purpose
High‐altitude pulmonary oedema (HAPE) experienced under high‐altitude conditions is attributed to mitochondrial redox distress. Hence, hypobaric hypoxia (HH)‐induced alteration ...in expression of mitochondrial biogenesis and dynamics genes was determined in rat lung. Further, such alteration was correlated with expression of mitochondrial DNA (mtDNA)‐encoded oxidative phosphorylation (mtOXPHOS) genes. The prophylactic effect of dexamethasone (DEX) in counteracting the HH‐induced mitochondrial distress was used as control to understand adaptation to high‐altitude exposure.
Experimental Approach
Rats pretreated with DEX were exposed to normobaric normoxia (NN) or HH. HH‐induced injury was assessed as an increase in lung water content, tissue damage and oxidant generation. Mitochondrial number, mtDNA content and mtOXPHOS activities were measured to determine mitochondrial function. The expression of mitochondrial biogenesis, dynamics and mtOXPHOS genes was studied.
Key Results
HH‐induced lung injury was associated with decreased mitochondrial number, mtDNA content and mtOXPHOS activities. HH exposure decreased the nuclear gene oestrogen‐related receptor‐α (ERRα), which interacts with PPAR‐γ coactivator‐1α (PGC‐1α) in controlling mitochondrial metabolism. Consequently, mtOXPHOS transcripts are repressed under HH. Further, HH modulated mitochondrial dynamics by decreasing mitofusin 2 (Mfn2) and augmenting fission 1 (Fis1) and dynamin‐related protein 1 (Drp1) expression. Nevertheless, DEX treatment under NN (i.e. adaptation to HH) did not affect mitochondrial biogenesis and dynamics, but increased mtOXPHOS transcripts. Further, mtOXPHOS activities increased together with reduced oxidant generation. Also, DEX pretreatment normalized ERRα along with mitochondrial dynamics genes and increased mtOXPHOS transcripts to elicit the mitochondrial function under HH.
Conclusions and Implications
HH stress (HAPE)‐mediated mitochondrial dysfunction is due to repressed ERRα and mtOXPHOS transcripts. Thus, ERRα‐mediated protection of mitochondrial bioenergetics might be the likely candidate required for lung adaptation to HH.
Endothelin 1 (EDN1) encodes a potent endogenous vasoconstrictor, ET1, to maintain vascular homeostasis and redistribution of tissue blood flow during exercise. One of the EDN1 missense polymorphisms, ...rs5370 G/T, has strongly been associated with cardiopulmonary diseases. This study investigated the impact of rs5370 polymorphism in high-altitude pulmonary oedema (HAPE) disorder or maladaptation and adaptation physiology in a well-characterized case–control study of high-altitude and low-altitude populations comprising 310 samples each of HAPE-patients, HAPE-free controls and native highlanders. The rs5370 polymorphism was genotyped, and the gene expression and plasma level of EDN1 were evaluated. The functional relevance of each allele was investigated in the human embryonic kidney 293 cell line after exposure to hypoxia and computationally. The T allele was significantly more prevalent in HAPE-p compared to HAPE-f and HLs. The EDN1 gene expression and ET1 bio-level were significantly elevated in HAPE-p compared to controls. Compared to the G allele, the T allele was significantly associated with elevated levels of ET-1 in all three study groups and cells exposed to hypoxia. The in silico studies further confirmed the stabilizing effect of the T allele on the structural integrity and function of ET1 protein. The ET1 rs5370 T allele is associated with an increased concentration of ET-1 in vivo and in vitro, establishing it as a potent marker in the adaptation/maladaptation physiology under the high-altitude environment. This could also be pertinent in endurance exercises at high altitudes.
ABSTRACT
Background and objective: The role of β2‐adrenergic receptor (ADRB2) in pulmonary oxygenation has been ascertained during altitude acclimatization, physical performance and lung fluid ...clearance, but little is known about its association with high‐altitude pulmonary oedema (HAPE), a non‐cardiogenic pulmonary oedema.
Methods: In a case–control study, 110 unrelated HAPE patients (HAPE‐p) and 143 unrelated HAPE‐resistant (HAPE‐r) controls matched on age and ethnicity were used to examine the association between eight single nucleotide polymorphisms (SNP) and disease. The eight SNP including three tag‐SNP were genotyped from promoter and exonic regions of ADRB2. Robust methods for predicting geneotype–phenotype interactions, for example, multidimensional reduction (MDR) and moving‐window haplotype analysis were applied.
Results: The haplotypes from 46A/G and 79C/G SNP of ADRB2 were associated with HAPE. The MDR model depicting disease association through genotype–genotype and genotype–phenotype interaction included SNP 46A/G, 79C/G and 523C/A. Its haplotype 46G_79C_523C was significantly overrepresented in HAPE‐r (P = 0.0001; χ2 = 14.95; OR = 4.52; 95% CI: 1.98–10.3). The global haplotype test showed significant association with HAPE (LRχ2 = 86.69, P < 0.0001). A moving‐window analysis revealed that haplotype –367C/T_46A/G_79C/G differed significantly between HAPE‐p and HAPE‐r (LRχ2 = 22.5, P = 0.002). The MDR model depicted SNP 46A/G, 79C/G and 523C/A as the best combination predicting disease.
Conclusions: The haplotypes of ADRB2 consisting of the SNP, 46A/G and 79C/G, have a greater power for predicting HAPE.
This case–control study examined the association between eight important single nucleotide polymorphisms (SNP) of ADRB2 and high‐altitude pulmonary oedema (HAPE). Methods for predicting geneotype–phenotype interactions such as multidimensional reduction and moving‐window haplotype analysis were applied. The haplotypes consisting of common SNP, 46A/G and 79C/G were associated with HAPE.
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