Summary
It is now largely admitted that a pro‐inflammatory environment may curtail anti‐tumor immunity and favor cancer initiation and progression. The discovery that small non‐coding regulatory ...RNAs, namely microRNAs (miRNAs), regulate all aspects of cell proliferation, differentiation, and function has shed a new light on regulatory mechanisms linking inflammation and cancer. Thus, miRNAs such as miR‐21, miR‐125b, miR‐155, miR‐196, and miR‐210 that are critical for the immune response or hypoxia are often overexpressed in cancers and leukemias. Given the high number of their target transcripts, their deregulation may have a number of deleterious consequences, depending on the cellular context. In this review, we focus on how the factors encoded by transcripts targeted by these five miRNAs, be they transcription factors, tumor‐suppressors, or regulators of different signaling pathways, can deregulate the immune response and favor pro‐tumor immunity. Furthermore, we expose how the misdirected action of the main regulators of these miRNAs, such as nuclear factor κB (NF‐κB), activator protein‐1 (AP‐1), and signal transduction and activators of transcription (STAT) transcription factors, or AKT and transforming growth factor β (TGFβ) signaling pathways, can contribute to decrease anti‐tumor immunity and enhance cell proliferation and oncogenesis. We conclude by briefly discussing about how these discoveries may possibly lead to the development of new miRNA‐based cancer therapies.
Functional mechanisms of miR‐192 family in cancer Mishan, Mohammad Amir; Tabari, Mohammad Amin Khazeei; Parnian, Javad ...
Genes chromosomes & cancer,
December 2020, Letnik:
59, Številka:
12
Journal Article
Recenzirano
By growing research on the mechanisms and functions of microRNAs (miRNAs, miRs), the role of these noncoding RNAs gained more attention in healthcare. Due to the remarkable regulatory role of miRNAs, ...any dysregulation in their expression causes cellular functional impairment. In recent years, it has become increasingly apparent that these small molecules contribute to development, cell differentiation, proliferation, apoptosis, and tumor growth. In many studies, the miR‐192 family has been suggested as a potential prognostic and diagnostic biomarker and even as a possible therapeutic target for several cancers. However, the mechanistic effects of the miR‐192 family on cancer cells are still controversial. Here, we have reviewed each family member of the miR‐192 including miR‐192, miR‐194, and miR‐215, and discussed their mechanistic roles in various cancers.
Objectives
This study aimed to experimentally validate dysregulated expression of miRNA candidates selected through updated meta‐analysis of most commonly deregulated miRNAs in oral cancer and to ...explore their diagnostic and prognostic potential.
Materials and methods
Five miRNAs (miR‐31‐3p, miR‐135b‐5p, miR‐18a‐5p, miR‐30a‐5p and miR‐139‐5p) from updated meta‐signature were selected for validation by qRT‐PCR method in 35 oral cancer clinical specimens and adjacent non‐cancerous tissue.
Results
Updated meta‐analysis has identified 13 most commonly deregulated miRNAs in oral cancer. Seven miRNAs were consistently up‐regulated (miR‐21‐5p, miR‐31‐3p, miR‐135b‐5p, miR‐31‐5p, miR‐424‐5p, miR‐18a‐5p and miR‐21‐3p), while five were down‐regulated (miR‐139‐5p, miR‐30a‐3p, miR‐375‐3p, miR‐376c‐3p and miR‐30a‐5p). Increased expression of miR‐31‐3p and miR‐135b‐5p, and decreased expression of miR‐139‐5p and miR‐30a‐5p were confirmed in oral cancer compared to adjacent non‐cancerous tissue. A three miRNAs combination (miR‐31‐3p, miR‐139‐5p and miR‐30a‐5p) gave the most promising diagnostic potential for discriminating oral cancer from non‐cancerous tissue (AUC: 0.780 95% CI: 0.673–0.886, p < 0.0005, sensitivity 94.3%, specificity 51.4%). High expression of miR‐135b‐5p, miR‐18a‐5p and miR‐30a‐5p was associated with poor survival (p = 0.003, p = 0.048, p = 0.016 respectively).
Conclusion
miR‐31‐3p, miR‐139‐5p and miR‐30a‐5p panel was confirmed as a potential diagnostic biomarker when distinguishing oral cancer from non‐cancerous tissue. miR‐135b‐5p, miR‐18a‐5p and miR‐30a‐5p might serve as potential biomarkers of poor survival of oral cancer patients.
Induced osteogenesis includes a program of microRNAs (miRs) to repress the translation of genes that act as inhibitors of bone formation. How expression of bone-related miRs is regulated remains a ...compelling question. Here we report that Runx2, a transcription factor essential for osteoblastogenesis, negatively regulates expression of the miR cluster 23a~27a~24-2. Overexpression, reporter, and chromatin immunoprecipitation assays established the presence of a functional Runx binding element that represses expression of these miRs. Consistent with this finding, exogenous expression of each of the miRs suppressed osteoblast differentiation, whereas antagomirs increased bone marker expression. The biological significance of Runx2 repression of this miR cluster is that each miR directly targets the 3' UTR of SATB2, which is known to synergize with Runx2 to facilitate bone formation. The findings suggest Runx2-negative regulation of multiple miRs by a feed-forward mechanism to cause derepression of SATB2 to promote differentiation. We find also that miR-23a represses Runx2 in the terminally differentiated osteocyte, representing a feedback mechanism to attenuate osteoblast maturation. We provide direct evidence for an interdependent relationship among transcriptional inhibition of the miR cluster by Runx2, translational repression of Runx2 and of SATB2 by the cluster miRs during progression of osteoblast differentiation. Furthermore, miR cluster gain of function (i.e., inhibition of osteogenesis) is rescued by the exogenous expression of SATB2. Taken together, we have established a regulatory network with a central role for the miR cluster 23a~27a~24-2 in both progression and maintenance of the osteocyte phenotype.
Background
Ethanol (EtOH) and nicotine are often co‐abused. However, their combined effects on fetal neural development, particularly on fetal neural stem cells (NSCs), which generate most neurons of ...the adult brain during the second trimester of pregnancy, are poorly understood. We previously showed that EtOH influenced NSC maturation in part, by suppressing the expression of specific microRNAs (miRNAs). Here, we tested in fetal NSCs the extent to which EtOH and nicotine coregulated known EtOH‐sensitive (miR‐9, miR‐21, miR‐153, and miR‐335), a nicotine‐sensitive miRNA (miR‐140‐3p), and mRNAs for nicotinic acetylcholine receptor (nAChR) subunits. Additionally, we tested the extent to which these effects were nAChR dependent.
Methods
Gestational day 12.5 mouse fetal murine cerebral cortical–derived neurosphere cultures were exposed to EtOH, nicotine, and mecamylamine, a noncompetitive nAChR antagonist, individually or in combination, for short (24 hour) and long (5 day) periods, to mimic exposure during the in vivo period of neurogenesis. Levels of miRNAs, miRNA‐regulated transcripts, and nAChR subunit mRNAs were assessed by quantitative reverse transcription polymerase chain reaction.
Results
EtOH suppressed the expression of known EtOH‐sensitive miRNAs and miR‐140‐3p, while nicotine at concentrations attained by cigarette smokers induced a dose‐related increase in these miRNAs. Nicotine's effect was blocked by EtOH and by mecamylamine. Finally, EtOH decreased the expression of nAChR subunit mRNAs and, like mecamylamine, prevented the nicotine‐associated increase in α4 and β2 nAChR transcripts.
Conclusions
EtOH and nicotine exert mutually antagonistic, nAChR‐mediated effects on teratogen‐sensitive miRNAs in fetal NSCs. These data suggest that concurrent exposure to EtOH and nicotine disrupts miRNA regulatory networks that are important for NSC maturation.
Beneficial microbes induce resistance in plants (MIR), imposing both lethal and sublethal effects on herbivorous insects. We argue that herbivores surviving MIR carry metabolic and immunological ...imprints of MIR with cascading effects across food webs. We propose that incorporating such cascading effects will strongly enhance the current MIR research framework.
Beneficial microbes induce resistance in plants (MIR), imposing both lethal and sublethal effects on herbivorous insects. We argue that herbivores surviving MIR carry metabolic and immunological imprints of MIR with cascading effects across food webs. We propose that incorporating such cascading effects will strongly enhance the current MIR research framework.
Right ventricular (RV) failure is the primary cause of death in pulmonary arterial hypertension (PAH). We hypothesized that heart‐relevant microRNAs, that is myomiRs (miR‐1, miR‐133a, miR‐208, ...miR‐499) and miR‐214, can have a role in the right ventricle in the development of PAH. To mimic PAH, male Wistar rats were injected with monocrotaline (MCT, 60 mg/kg, s.c.); control group received vehicle. MCT rats were divided into two groups, based on the clinical presentation: MCT group terminated 4 weeks after MCT administration and prematurely terminated group (ptMCT) displaying signs of terminal disease. Myocardial damage genes and candidate microRNAs expressions were determined by RT‐qPCR. Reduced blood oxygen saturation, breathing disturbances, RV enlargement as well as elevated levels of markers of myocardial damage confirmed PH in MCT animals and were more pronounced in ptMCT. MyomiRs (miR‐1/miR‐133a/miR‐208a/miR‐499) were decreased and the expression of miR‐214 was increased only in ptMCT group (P < 0.05). The myomiRs negatively correlated with Fulton index as a measure of RV hypertrophy in MCT group (P < 0.05), whereas miR‐214 showed a positive correlation (P < 0.05). We conclude that the expression of determined microRNAs mirrored the disease severity and targeting their pathways might represent potential future therapeutic approach in PAH.
Myeloid‐derived suppressor cells (MDSCs) play a pivotal role in mediating the formation of an immunosuppressive environment and assisting tumors in evading the host immune response. However, the ...mechanism through which tumors manipulate the differentiation and function of MDSCs remains unclear. Here, we report that hypoxia‐induced glioma cells can stimulate the differentiation of functional MDSCs by transferring exosomal miR‐29a and miR‐92a to MDSCs. Our results showed that glioma‐derived exosomes (GEXs) can enhance the differentiation of functional MDSCs both in vitro and in vivo, and hypoxia‐induced GEXs (H‐GEXs) demonstrated a stronger MDSCs induction ability than did normoxia‐induced GEXs (N‐GEXs). A subsequent miRNA sequencing analysis of N‐GEXs and H‐GEXs revealed that hypoxia‐induced exosomal miR‐29a and miR‐92a expression induced the propagation of MDSCs. miR‐29a and miR‐92a activated the proliferation and function of MDSCs by targeting high‐mobility group box transcription factor 1 (Hbp1) and protein kinase cAMP‐dependent type I regulatory subunit alpha (Prkar1a), respectively. Altogether, the results of our study provide new insights into the role of glioma exosomal miRNAs in mediating the formation of immunosuppressive microenvironments in tumors and elucidate the underlying exosomal miR‐29a/miR‐92a‐based regulatory mechanism responsible for the modulation of functional MDSC induction.
What's new?
Myeloid derived suppressor cells (MDSC) suppress the immune system, reducing the effectiveness of immunotherapy in various cancers. These authors investigated how hypoxia promotes the immunosuppressive activity of MDSCs. Glioma cells, they found, use exosomes to transport miRNAs to MDSC progenitor cells, stimulating their differentiation. Hypoxia‐induced exosomes exerted a more powerful effect on MDSC proliferation than normoxia‐induced exosomes. The miRNAs induced MDSCs by targeting two genes, Hbp1 and Prkar1a. miRNA silencing of Hbp1 induced the cell cycle progression of MDSCs. This is the first study to demonstrate a mechanism by which glioma cells stimulate MDSC expansion.
Tumorigenic environments, especially aberrantly overexpressed oncogenic microRNAs, play a critical role in various activities of tumor progression. However, developing strategies to effectively ...utilize and manipulate these oncogenic microRNAs for tumor therapy is still a challenge. To address this challenge, spherical nucleic acids (SNAs) consisting of gold nanoparticles in the core and antisense oligonucleotides as the shell are fabricated. Hybridized to the oligonucleotide shell is a DNA sequence to which doxorubicin is conjugated (DNA‐DOX). The oligonucleotides shell is designed to capture overexpressed miR‐21/miR‐155 and inhibit the expression of these oncogenic miRNAs in tumor cells after tumor accumulation to manipulate genetic environment for accurate gene therapy. This process further induces the aggregation of these SNAs, which not only generates photothermal agents to achieve on‐demand photothermal therapy in situ, but also enlarges the size of SNAs to enhance the retention time in the tumor for sustained therapy. The capture of the relevant miRNAs simultaneously triggers the intracellular release of the DNA‐DOX from the SNAs to deliver tumor‐specific chemotherapy. Both in vivo and in vitro results indicate that this combination strategy has excellent tumor inhibition properties with high survival rate of tumor‐bearing mice, and can thus be a promising candidate for effective tumor treatment.
Multiple functional spherical nucleic acids are developed to utilize and manipulate the tumorigenic genetic environment, and are able to perform tumor‐specific chain activities including oncogenic miRNAs (miR‐21 and miR‐155) inhibition, miR‐21‐dependent photothermal therapy, and miR‐155‐triggered chemotherapy for effective, accurate, and sustained tumor treatment.
One of the most prevalent neurodegenerative disorders is Alzheimer's disease (AD). Despite the pervasiveness of AD being considerable, the rates of both diagnosis and therapy are comparatively less ...and still lacking. For the treatment of AD, acetylcholinesterase inhibitors and NMDA receptor antagonists (Memantine) have received clinical approval. The approved drugs are only capable of mitigating the symptoms; however, halting the progression of the disease remains a matter of substantial concern. MicroRNAs (miRs) are a subclass of non-coding single-stranded RNA molecules that target mRNAs to control the expression of genes in certain tissues. Dysregulation in the expression and function of miRs contributes to a neurodegeneration-like pathogenesis seen in Alzheimer's disease (AD), featuring hallmark characteristics such as Aβ aggregation, hyper-phosphorylation of Tau proteins, mitochondrial dysfunction, neuroinflammation, and apoptosis. These factors collectively underpin the cognitive deterioration and learning disabilities associated with AD. According to the research, numerous miRs have considerably different expression patterns in AD patients compared to healthy people. Due to these attributes, miRs prove to be effective diagnostic and therapeutic agents for AD. This review will examine clinical and preclinical data concerning the potential of miRs as diagnostic and therapeutic agents, utilizing various techniques (such as miR antagonists or inhibitors, miR agonists or mimics, miR sponges, and miR antisense oligonucleotides) to target specific pathogenic mechanisms in AD.
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