Imaging mass spectrometry (IMS) technologies are capable of mapping a wide array of biomolecules in diverse cellular and tissue environments. IMS has emerged as an essential tool for providing ...spatially targeted molecular information due to its high sensitivity, wide molecular coverage, and chemical specificity. One of the major challenges for mapping the complex cellular milieu is the presence of many isomers and isobars in these samples. This challenge is traditionally addressed using orthogonal liquid chromatography (LC)‐based analysis, though, common approaches such as chromatography and electrophoresis are not able to be performed at timescales that are compatible with most imaging applications. Ion mobility offers rapid, gas‐phase separations that are readily integrated with IMS workflows in order to provide additional data dimensionality that can improve signal‐to‐noise, dynamic range, and specificity. Here, we highlight recent examples of ion mobility coupled to IMS and highlight their importance to the field.
L'article met à l'épreuve une approche écopoétique sur un corpus de cinq écrits viatiques rédigés entre 1656 et 1683 par Chapelle et Bachaumont, La Fontaine, Claude Perrault, Dassoucy et Préchac, ces ...écrits étant resserrés sur le périmètre géographique de la France. Le voyage y est envisagé comme moteur d'une attention variable selon les objets extérieurs ciblés et son intensité : l'approche écopoétique mesure ces variations, tout comme la mise en œuvre d'une expérience plaçant l'individu au contact d'un environnement inhabituel. L'engagement du sujet voyageur, le recul qu'impose une écriture et les particularités d'un itinéraire sont analysés comme des opérateurs de signification.
We make use of newly available data that include roughly 5 million linked household and population records from 1850 to 2015 to document long-term trends in intergenerational social mobility in the ...United States. Intergenerational mobility declined substantially over the past 150 y, but more slowly than previously thought. Intergenerational occupational rank–rank correlations increased from less than 0.17 to as high as 0.32, but most of this change occurred to Americans born before 1900. After controlling for the relatively high mobility of persons from farm origins, we find that intergenerational social mobility has been remarkably stable. In contrast with relative stability in rank-based measures of mobility, absolute mobility for the nonfarm population—the fraction of offspring whose occupational ranks are higher than those of their parents—increased for birth cohorts born prior to 1900 and has fallen for those born after 1940.
Mass spectrometry-based biomolecular analyses have become permanent fixtures of academic, industrial, and clinical research settings. The rise in utilization of mass spectrometry has, in turn, ...spurred on a technological arms race, with every major vendor seeking to provide instrumentation that is more sensitive, higher in resolution, or may otherwise offer fundamental advantages during analysis. Enabling higher sensitivity, increased instrumental duty cycle, reduced analysis time and lower sample requirements, gas phase ion separation techniques now provide a fourth dimension of analysis, enabling rapid structural characterization and high throughput -omics profiling in a single run. Presented here is a current review of the latest iterations and applications of high-end ion-mobility enabled instrumentation, the Agilent 6560 IM-QTOF, Waters Cyclic, Bruker timsTOF, and Thermo FAIMS Pro instrument platforms. Describing their engineering developments and analytical successes over the past two decades, we highlight notable advantages and considerations for novice and experienced biomolecular researchers alike.
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•Current state of high-end ion mobility mass spectrometry is assessed and reviewed.•Capacity for structural characterization and omics applications of four major IMS platforms are highlighted.•Latest generation of DTIMS, TWIMS, TIMS, and FAIMS have been reviewed for their instrumental developments and applications.•Current limitations and future perspectives for each IMS regime are discussed.
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